Scientific Commons: Zbigniew Dominski

Two Xenopus Proteins That Bind the 3′ End of Histone mRNA: Implications for Translational Control of Histone Synthesis during Oogenesis

Wang, Zeng-Feng, Ingledue, Thomas C., Dominski, Zbigniew, Sanchez, Ricardo, Marzluff, William F.

Translationally inactive histone mRNA is stored in frog oocytes, and translation is activated at oocyte maturation. The replication-dependent histone mRNAs are not polyadenylated and end in a...

Stem-Loop Binding Protein Facilitates 3′-End Formation by Stabilizing U7 snRNP Binding to Histone Pre-mRNA

Dominski, Zbigniew, Zheng, Lian-Xing, Sanchez, Ricardo, Marzluff, William F.

The 3′ end of histone mRNA is formed by an endonucleolytic cleavage of the primary transcript after a conserved stem-loop sequence. The cleavage reaction requires at least two trans-acting factors:...

Mutations in the RNA Binding Domain of Stem-Loop Binding Protein Define Separable Requirements for RNA Binding and for Histone Pre-mRNA Processing

Dominski, Zbigniew, Erkmann, Judith A., Greenland, John A., Marzluff, William F.

Expression of replication-dependent histone genes at the posttranscriptional level is controlled by stem-loop binding protein (SLBP). One function of SLBP is to bind the stem-loop structure in the...

3′ End Processing of Drosophila melanogaster Histone Pre-mRNAs: Requirement for Phosphorylated Drosophila Stem-Loop Binding Protein and Coevolution of the Histone Pre-mRNA Processing System

Dominski, Zbigniew, Yang, Xiao-cui, Raska, Christy S., Santiago, Carlos, Borchers, Christoph H., Duronio, Robert J., ...

Synthetic pre-mRNAs containing the processing signals encoded by Drosophila melanogaster histone genes undergo efficient and faithful endonucleolytic cleavage in nuclear extracts prepared from...

Phosphorylation of Stem-Loop Binding Protein (SLBP) on Two Threonines Triggers Degradation of SLBP, the Sole Cell Cycle-Regulated Factor Required for Regulation of Histone mRNA Processing, at the End of S Phase

Zheng, Lianxing, Dominski, Zbigniew, Yang, Xiao-Cui, Elms, Phillip, Raska, Christy S., Borchers, Christoph H., ...

The replication-dependent histone mRNAs, the only eukaryotic mRNAs that do not have poly(A) tails, are present only in S-phase cells. Coordinate posttranscriptional regulation of histone mRNAs is...

Cloning and characterization of the Drosophila U7 small nuclear RNA

Dominski, Zbigniew, Yang, Xiao-cui, Purdy, Matthew, Marzluff, William F.

Base pairing between the 5′ end of U7 small nuclear RNA (snRNA) and the histone downstream element (HDE) in replication-dependent histone pre-mRNAs is the key event in 3′-end processing that...

Drosophila Stem-Loop Binding Protein Intracellular Localization Is Mediated by Phosphorylation and Is Required for Cell Cycle-regulated Histone mRNA Expression

Lanzotti, David J., Kupsco, Jeremy M., Yang, Xiao-Cui, Dominski, Zbigniew, Marzluff, William F., Duronio, Robert J.

Stem-loop binding protein (SLBP) is an essential component of the histone pre-mRNA processing machinery. SLBP protein expression was examined during Drosophila development by using transgenes...

The sea urchin stem–loop-binding protein: a maternally expressed protein that probably functions in expression of multiple classes of histone mRNA

Robertson, Anthony J., Howard, Jason T., Dominski, Zbigniew, Schnackenberg, Bradley J., Sumerel, Jan L., McCarthy, John J., ...

Following the completion of oogenesis and oocyte maturation, histone mRNAs are synthesized and stored in the sea urchin egg pronucleus. Histone mRNAs are the only mRNAs that are not polyadenylated...

SLBP is associated with histone mRNA on polyribosomes as a component of the histone mRNP

Whitfield, Michael L., Kaygun, Handan, Erkmann, Judith A., Townley-Tilson, W. H. Davin, Dominski, Zbigniew, Marzluff, William F.

The stem–loop binding protein (SLBP) binds the 3′ end of histone mRNA and is present both in nucleus, and in the cytoplasm on the polyribosomes. SLBP participates in the processing of the histone...

A CPSF-73 Homologue Is Required for Cell Cycle Progression but Not Cell Growth and Interacts with a Protein Having Features of CPSF-100

Dominski, Zbigniew, Yang, Xiao-cui, Purdy, Matthew, Wagner, Eric J., Marzluff, William F.

Formation of the mature 3′ ends of the vast majority of cellular mRNAs occurs through cleavage and polyadenylation and requires a cleavage and polyadenylation specificity factor (CPSF) containing,...

Drosophila stem loop binding protein coordinates accumulation of mature histone mRNA with cell cycle progression

Sullivan, Eileen, Santiago, Carlos, Parker, Emily D., Dominski, Zbigniew, Yang, Xiaocui, Lanzotti, David J., ...

Replication-associated histone genes encode the only metazoan mRNAs that lack polyA tails, ending instead in a conserved 26-nt sequence that forms a stem–loop. Most of the regulation of mammalian...

A novel zinc finger protein is associated with U7 snRNP and interacts with the stem–loop binding protein in the histone pre-mRNP to stimulate 3′-end processing

Dominski, Zbigniew, Erkmann, Judith A., Yang, Xiaocui, Sànchez, Ricardo, Marzluff, William F.

The stem–loop binding protein (SLBP) is the posttranscriptional regulator of histone mRNA in metazoan cells. SLBP binds histone pre-mRNAs and facilitates 3′-end processing by promoting stable...

Differences and similarities between Drosophila and mammalian 3′ end processing of histone pre-mRNAs

DOMINSKI, ZBIGNIEW, YANG, XIAO-CUI, PURDY, MATHEW, MARZLUFF, WILLIAM F.

We used nuclear extracts from Drosophila Kc cells to characterize 3′ end processing of Drosophila histone pre-mRNAs. Drosophila SLBP plays a critical role in recruiting the U7 snRNP to the pre-mRNA...

Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem–loop-binding proteins that contributes to high-affinity RNA binding

Borchers, Christoph H., Thapar, Roopa, Petrotchenko, Evgeniy V., Torres, Matthew P., Speir, J. Paul, Easterling, Michael, ...

The stem–loop-binding protein (SLBP) is involved in multiple aspects of histone mRNA metabolism. To characterize the modification status and sites of SLBP, we combined mass spectrometric bottom-up...

U7 snRNA mutations in Drosophila block histone pre-mRNA processing and disrupt oogenesis

GODFREY, ASHLEY C., KUPSCO, JEREMY M., BURCH, BRANDON D., ZIMMERMAN, RYAN M., DOMINSKI, ZBIGNIEW, MARZLUFF, WILLIAM F., ...

Metazoan replication-dependent histone mRNAs are not polyadenylated, and instead terminate in a conserved stem–loop structure generated by an endonucleolytic cleavage involving the U7 snRNP, which...