Scientific Commons: Yves Henry

Stable expression in yeast of the mature form of human telomerase RNA depends on its association with the box H/ACA small nucleolar RNP proteins Cbf5p, Nhp2p and Nop10p

Dez, Christophe, Henras, Anthony, Faucon, Bruno, Lafontaine, Denis L. J., Caizergues-Ferrer, Michèle, Henry, Yves

Telomerase is a ribonucleoprotein (RNP) particle required for the replication of telomeres. The RNA component, termed hTR, of human telomerase contains a domain structurally and functionally related...

Accumulation of H/ACA snoRNPs depends on the integrity of the conserved central domain of the RNA-binding protein Nhp2p

Henras, Anthony, Dez, Christophe, Noaillac-Depeyre, Jacqueline, Henry, Yves, Caizergues-Ferrer, Michèle

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. How box H/ACA snoRNPs are assembled remains unknown. Here we show that...

Processing of the Precursors to Small Nucleolar RNAs and rRNAs Requires Common Components

Petfalski, Elisabeth, Dandekar, Thomas, Henry, Yves, Tollervey, David

The genes encoding the small nucleolar RNA (snoRNA) species snR190 and U14 are located close together in the genome of Saccharomyces cerevisiae. Here we report that these two snoRNAs are synthesized...

Seven Novel Methylation Guide Small Nucleolar RNAs Are Processed from a Common Polycistronic Transcript by Rat1p and RNase III in Yeast

Qu, Liang-Hu, Henras, Anthony, Lu, Yong-Jun, Zhou, Hui, Zhou, Wei-xin, Zhu, Yuan-Qi, ...

Through a computer search of the genome of the yeast Saccharomyces cerevisiae, the coding sequences of seven different box C/D antisense small nucleolar RNAs (snoRNAs) with the structural hallmarks...

Naf1p, an Essential Nucleoplasmic Factor Specifically Required for Accumulation of Box H/ACA Small Nucleolar RNPs†

Dez, Christophe, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Henry, Yves

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. The ways in which these particles are assembled and correctly localized...

Expression Profiling of the Whole Arabidopsis Shaggy-Like Kinase Multigene Family by Real-Time Reverse Transcriptase-Polymerase Chain Reaction1

Charrier, Bénédicte, Champion, Anthony, Henry, Yves, Kreis, Martin

The recent publication of the complete sequence of the Arabidopsis genome allowed us to identify and characterize the last two members of the SHAGGY-like kinase (AtSK) gene family. As a result, the...

The box H+ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase

Lafontaine, Denis L.J., Bousquet-Antonelli, Cécile, Henry, Yves, Caizergues-Ferrer, Michèle, Tollervey, David

Many or all of the sites of pseudouridine (Ψ) formation in eukaryotic rRNA are selected by site-specific base-pairing with members of the box H + ACA class of small nucleolar RNAs (snoRNAs)....

Npa1p, a Component of Very Early Pre-60S Ribosomal Particles, Associates with a Subset of Small Nucleolar RNPs Required for Peptidyl Transferase Center Modification†

Dez, Christophe, Froment, Carine, Noaillac-Depeyre, Jacqueline, Monsarrat, Bernard, Caizergues-Ferrer, Michèle, Henry, Yves

We have identified a novel essential nucleolar factor required for the synthesis of 5.8S and 25S rRNAs termed Npa1p. In the absence of Npa1p, the pre-rRNA processing pathway leading to 5.8S and 25S...

Cotranscriptional Recruitment of the Pseudouridylsynthetase Cbf5p and of the RNA Binding Protein Naf1p during H/ACA snoRNP Assembly

Yang, Pok Kwan, Hoareau, Coralie, Froment, Carine, Monsarrat, Bernard, Henry, Yves, Chanfreau, Guillaume

H/ACA small nucleolar ribonucleoprotein particles (snoRNPs) are essential for the maturation and pseudouridylation of the precursor of rRNAs and other stable RNAs. Although the RNA and protein...

The Splicing ATPase Prp43p Is a Component of Multiple Preribosomal Particles

Lebaron, Simon, Froment, Carine, Fromont-Racine, Micheline, Rain, Jean-Christophe, Monsarrat, Bernard, Caizergues-Ferrer, Michèle, ...

Prp43p is a putative helicase of the DEAH family which is required for the release of the lariat intron from the spliceosome. Prp43p could also play a role in ribosome synthesis, since it accumulates...

Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs

HENRAS, ANTHONY K., CAPEYROU, REGINE, HENRY, YVES, CAIZERGUES-FERRER, MICHELE

Box C/D and box H/ACA small ribonucleoprotein particles (sRNPs) are found from archaea to humans, and some of these play key roles during the biogenesis of ribosomes or components of the splicing...

hNaf1 is required for accumulation of human box H/ACA snoRNPs, scaRNPs, and telomerase

Hoareau-Aveilla, Coralie, Bonoli, Mattia, Caizergues-Ferrer, Michele, Henry, Yves

The human telomerase ribonucleoprotein particle (RNP) shares with box H/ACA small Cajal body (sca)RNPs and small nucleolar (sno)RNPs the proteins dyskerin, hGar1, hNhp2, and hNop10. How dyskerin,...