Yuri Motorin

Pseudouridine Mapping in the Saccharomyces cerevisiae Spliceosomal U Small Nuclear RNAs (snRNAs) Reveals that Pseudouridine Synthase Pus1p Exhibits a Dual Substrate Specificity for U2 snRNA and tRNA

Massenet, Séverine, Motorin, Yuri, Lafontaine, Denis L. J., Hurt, Eduard C., Grosjean, Henri, Branlant, Christiane

Pseudouridine (Ψ) residues were localized in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (UsnRNAs) by using the chemical mapping method. In contrast to vertebrate UsnRNAs, S....

Cloning and characterization of the Schizosaccharomyces pombe tRNA:pseudouridine synthase Pus1p

Hellmuth, Klaus, Grosjean, Henri, Motorin, Yuri, Deinert, Karina, Hurt, Ed, Simos, George

Saccharomyces cerevisiae cells that carry deletions in both the LOS1 (a tRNA export receptor) and the PUS1 (a tRNA:pseudouridine synthase) genes exhibit a thermosensitive growth defect. A...

The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite–multisubstrate RNA:Ψ-synthase also acting on tRNAs

BEHM-ANSMANT, ISABELLE, URBAN, ALAN, MA, XIAOJU, YU, YI-TAO, MOTORIN, YURI, BRANLANT, CHRISTIANE

The Saccharomyces cerevisiae Pus7 protein was recently characterized as a novel RNA:pseudouridine (Ψ)-synthase acting at position 35 in U2 snRNA. However, U2 snRNA was the only potential substrate...

Pseudouridine Mapping in the Saccharomyces cerevisiae Spliceosomal U Small Nuclear RNAs (snRNAs) Reveals that Pseudouridine Synthase Pus1p Exhibits a Dual Substrate Specificity for U2 snRNA and tRNA

Massenet, Séverine, Motorin, Yuri, Lafontaine, Denis L. J., Hurt, Eduard C., Grosjean, Henri, Branlant, Christiane

Pseudouridine (Ψ) residues were localized in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (UsnRNAs) by using the chemical mapping method. In contrast to vertebrate UsnRNAs, S....

Cloning and characterization of the Schizosaccharomyces pombe tRNA:pseudouridine synthase Pus1p

Hellmuth, Klaus, Grosjean, Henri, Motorin, Yuri, Deinert, Karina, Hurt, Ed, Simos, George

Saccharomyces cerevisiae cells that carry deletions in both the LOS1 (a tRNA export receptor) and the PUS1 (a tRNA:pseudouridine synthase) genes exhibit a thermosensitive growth defect. A...

The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite–multisubstrate RNA:Ψ-synthase also acting on tRNAs

BEHM-ANSMANT, ISABELLE, URBAN, ALAN, MA, XIAOJU, YU, YI-TAO, MOTORIN, YURI, BRANLANT, CHRISTIANE

The Saccharomyces cerevisiae Pus7 protein was recently characterized as a novel RNA:pseudouridine (Ψ)-synthase acting at position 35 in U2 snRNA. However, U2 snRNA was the only potential substrate...

A previously unidentified activity of yeast and mouse RNA:pseudouridine synthases 1 (Pus1p) on tRNAs

Behm-Ansmant, Isabelle, Massenet, Séverine, Immel, FranÇoise, Patton, Jeffrey R., Motorin, Yuri, Branlant, Christiane

Mouse pseudouridine synthase 1 (mPus1p) was the first vertebrate RNA:pseudouridine synthase that was cloned and characterized biochemically. The mPus1p was previously found to catalyze Ψ formation...

The Saccharomyces cerevisiae Pus2 protein encoded by YGL063w ORF is a mitochondrial tRNA:Ψ27/28-synthase

Behm-Ansmant, Isabelle, Branlant, Christiane, Motorin, Yuri

The RNA:pseudouridine (Ψ)-synthase family is one of the most complex families of RNA modification enzymes. Ten genes encoding putative RNA:Ψ-synthases have been identified in S. cerevisiae. Most of...