Yamagata, Kazutsune, Kato, Jun-ichi, Shimamoto, Akira, Goto, Makoto, Furuichi, Yasuhiro, Ikeda, Hideo
Bloom’s syndrome (BS) and Werner’s syndrome (WS) are genetic disorders in which an increased rate of chromosomal aberration is detected. The genes responsible for these diseases, BLM and WRN,...
Niida, Hiroyuki, Shinkai, Yoichi, Hande, M. Prakash, Matsumoto, Takehisa, Takehara, Shoko, Tachibana, Makoto, ...
Telomere dynamics, chromosomal instability, and cellular viability were studied in serial passages of mouse embryonic stem (ES) cells in which the telomerase RNA (mTER) gene was deleted. These cells...
Shimamoto, Akira, Nishikawa, Kaori, Kitao, Saori, Furuichi, Yasuhiro
The RecQ helicase superfamily has been implicated in DNA repair and recombination. At least five human RecQ-related genes exist: RecQ1, BLM, WRN, RecQ4 and RecQ5. Mutations in BLM, WRN and RecQ4 are...
Sp1-Mediated Transcription of the Werner Helicase Gene Is Modulated by Rb and p53
Yamabe, Yukako, Shimamoto, Akira, Goto, Makoto, Yokota, Jun, Sugawara, Minoru, Furuichi, Yasuhiro
The regulation of Werner’s syndrome gene (WRN) expression was studied by characterizing the cis-regulatory elements in the promoter region and the trans-activating factors that bind to them. First,...
Ohsugi, Itaru, Tokutake, Yoshiki, Suzuki, Noriyuki, Ide, Toshinori, Sugimoto, Masanobu, Furuichi, Yasuhiro
We describe the unique structural features of a large telomere repeat DNA complex (TRDC) of >20 kb generated by a simple PCR using (TTAGGG)4 and (CCCTAA)4 as both primers and templates. Although...
Incorrect Use of “Immortalization” for B-Lymphoblastoid Cell Lines Transformed by Epstein-Barr Virus
Sugimoto, Masanobu, Furuichi, Yasuhiro, Ide, Toshinori, Goto, Makoto
Kuchino, Yoshiyuki, Nishimura, Susumu, Smith, Robert E., Furuichi, Yasuhiro
The 3′-terminal regions (20 to 32 residues) of the genome double-stranded RNA (dsRNA) segments of cytoplasmic polyhedrosis virus were sequenced. The dsRNAs, which were labeled at their 3′ termini...
Chemical Methylation of RNA and DNA Viral Genomes as a Probe of In Situ Structure
Yamakawa, Minoru, Shatkin, Aaron J., Furuichi, Yasuhiro
We used [methyl-3H] dimethyl sulfate to probe the genome structures of several RNA and DNA viruses. We compared sites of modification in nucleic acids that were methylated chemically before and after...
Regulation of CDK4 activity by a novel CDK4-binding protein, p34SEI-1
Sugimoto, Masataka, Nakamura, Takeshi, Ohtani, Naoko, Hampson, Lynne, Hampson, Ian N., Shimamoto, Akira, ...
The p16INK4a tumor suppressor inhibits cyclin-dependent kinases (CDK4 and CDK6). Here we report the isolation of a novel gene, SEI-1, whose product (p34SEI-1) appears to antagonize the function of...
S-adenosyl-L-methionine (SAM) activated the virus-associated RNA polymerase of cytoplasmic polyhedrosis virus in vitro. Synthesis of single-stranded viral RNA (mRNA) proceeded depending on the...
“Pretranscriptional capping” in the biosynthesis of cytoplasmic polyhedrosis virus mRNA
The in vitro synthesis of cytoplasmic polyhedrosis virus (CPV) mRNA was previously shown to be dependent upon the presence of the methyl donor S-adenosylmethionine (AdoMet). We now find that the...
Exportin-5 orthologues are functionally divergent among species
Shibata, Satoshi, Sasaki, Mitsuho, Miki, Takashi, Shimamoto, Akira, Furuichi, Yasuhiro, Katahira, Jun, ...
Exportin-5, an evolutionarily conserved nuclear export factor belonging to the importin-β family of proteins, is known to play a role in the nuclear export of small noncoding RNAs such as precursors...
Yamagata, Kazutsune, Kato, Jun-ichi, Shimamoto, Akira, Goto, Makoto, Furuichi, Yasuhiro, Ikeda, Hideo
Bloom’s syndrome (BS) and Werner’s syndrome (WS) are genetic disorders in which an increased rate of chromosomal aberration is detected. The genes responsible for these diseases, BLM and WRN,...
Niida, Hiroyuki, Shinkai, Yoichi, Hande, M. Prakash, Matsumoto, Takehisa, Takehara, Shoko, Tachibana, Makoto, ...
Telomere dynamics, chromosomal instability, and cellular viability were studied in serial passages of mouse embryonic stem (ES) cells in which the telomerase RNA (mTER) gene was deleted. These cells...
Shimamoto, Akira, Nishikawa, Kaori, Kitao, Saori, Furuichi, Yasuhiro
The RecQ helicase superfamily has been implicated in DNA repair and recombination. At least five human RecQ-related genes exist: RecQ1, BLM, WRN, RecQ4 and RecQ5. Mutations in BLM, WRN and RecQ4 are...
Sp1-Mediated Transcription of the Werner Helicase Gene Is Modulated by Rb and p53
Yamabe, Yukako, Shimamoto, Akira, Goto, Makoto, Yokota, Jun, Sugawara, Minoru, Furuichi, Yasuhiro
The regulation of Werner’s syndrome gene (WRN) expression was studied by characterizing the cis-regulatory elements in the promoter region and the trans-activating factors that bind to them. First,...
Ohsugi, Itaru, Tokutake, Yoshiki, Suzuki, Noriyuki, Ide, Toshinori, Sugimoto, Masanobu, Furuichi, Yasuhiro
We describe the unique structural features of a large telomere repeat DNA complex (TRDC) of >20 kb generated by a simple PCR using (TTAGGG)4 and (CCCTAA)4 as both primers and templates. Although...
Incorrect Use of “Immortalization” for B-Lymphoblastoid Cell Lines Transformed by Epstein-Barr Virus
Sugimoto, Masanobu, Furuichi, Yasuhiro, Ide, Toshinori, Goto, Makoto
Kuchino, Yoshiyuki, Nishimura, Susumu, Smith, Robert E., Furuichi, Yasuhiro
The 3′-terminal regions (20 to 32 residues) of the genome double-stranded RNA (dsRNA) segments of cytoplasmic polyhedrosis virus were sequenced. The dsRNAs, which were labeled at their 3′ termini...
Chemical Methylation of RNA and DNA Viral Genomes as a Probe of In Situ Structure
Yamakawa, Minoru, Shatkin, Aaron J., Furuichi, Yasuhiro
We used [methyl-3H] dimethyl sulfate to probe the genome structures of several RNA and DNA viruses. We compared sites of modification in nucleic acids that were methylated chemically before and after...
Regulation of CDK4 activity by a novel CDK4-binding protein, p34SEI-1
Sugimoto, Masataka, Nakamura, Takeshi, Ohtani, Naoko, Hampson, Lynne, Hampson, Ian N., Shimamoto, Akira, ...
The p16INK4a tumor suppressor inhibits cyclin-dependent kinases (CDK4 and CDK6). Here we report the isolation of a novel gene, SEI-1, whose product (p34SEI-1) appears to antagonize the function of...
S-adenosyl-L-methionine (SAM) activated the virus-associated RNA polymerase of cytoplasmic polyhedrosis virus in vitro. Synthesis of single-stranded viral RNA (mRNA) proceeded depending on the...
“Pretranscriptional capping” in the biosynthesis of cytoplasmic polyhedrosis virus mRNA
The in vitro synthesis of cytoplasmic polyhedrosis virus (CPV) mRNA was previously shown to be dependent upon the presence of the methyl donor S-adenosylmethionine (AdoMet). We now find that the...
Exportin-5 orthologues are functionally divergent among species
Shibata, Satoshi, Sasaki, Mitsuho, Miki, Takashi, Shimamoto, Akira, Furuichi, Yasuhiro, Katahira, Jun, ...
Exportin-5, an evolutionarily conserved nuclear export factor belonging to the importin-β family of proteins, is known to play a role in the nuclear export of small noncoding RNAs such as precursors...
Selvakumar, Dakshnamurthy, Miyamoto, Masahiko, Furuichi, Yasuhiro, Komiyama, Tadazumi
Single-chain variable-fragment (scFv) anti-idiotypic antibodies of an HM-1 killer toxin (HM-1) from the yeast Williopsis saturnus var. mrakii IFO 0895 have been produced by recombinant DNA technology...
Shiratori, Miwa, Sakamoto, Sakae, Suzuki, Noriyuki, Tokutake, Yoshiki, Kawabe, Yoichi, Enomoto, Takemi, ...
We prepared several monoclonal antibodies (mAbs) specific for the NH2- and COOH-terminal regions of the DNA helicase (WRN helicase) responsible for Werner's syndrome known as a premature aging...