Darzacq, Xavier, Tucker, Karen E., Matera, A.Gregory, Bertrand, Edouard, Kiss, Tam S
Biogenesis of functional spliceosomal small nuclear RNAs (snRNAs) includes the post-transcriptional covalent modification of numerous internal nucleotides. We have recently demonstrated that...
Nucleolar Factors Direct the 2′-O-Ribose Methylation and Pseudouridylation of U6 Spliceosomal RNA
Ganot, Philippe, Jády, Beáta E., Bortolin, Marie-Line, Darzacq, Xavier, Kiss, Tamás
The nucleolus has long been known as a functionally highly specialized subnuclear compartment where synthesis, posttranscriptional modification, and processing of cytoplasmic rRNAs take place. In...
Processing of Intron-Encoded Box C/D Small Nucleolar RNAs Lacking a 5′,3′-Terminal Stem Structure
The C and D box-containing (box C/D) small nucleolar RNAs (snoRNAs) function in the nucleolytic processing and 2′-O-methylation of precursor rRNA. In vertebrates, most box C/D snoRNAs are processed...
Darzacq, Xavier, Jády, Beáta E., Verheggen, Céline, Kiss, Arnold M., Bertrand, Edouard, Kiss, Tamás
Cajal (coiled) bodies are conserved subnuclear organelles that are present in the nucleoplasm of both animal and plant cells. Although Cajal bodies were first described nearly 100 years ago, their...
A Cajal body-specific pseudouridylation guide RNA is composed of two box H/ACA snoRNA-like domains
Kiss, Arnold M., Jády, Beáta E., Darzacq, Xavier, Verheggen, Céline, Bertrand, Edouard, Kiss, Tamás
Site-specific post-transcriptional conversion of uridines to pseudouridine in ribosomal RNAs and small nuclear RNAs (snRNAs) is directed by guide RNAs which possess the conserved box H and ACA...
Jády, Beáta E., Darzacq, Xavier, Tucker, Karen E., Matera, A.Gregory, Bertrand, Edouard, Kiss, Tamás
Biogenesis of functional spliceosomal small nuclear RNAs (snRNAs) includes the post-transcriptional covalent modification of numerous internal nucleotides. We have recently demonstrated that...
A common sequence motif determines the Cajal body-specific localization of box H/ACA scaRNAs
Richard, Patricia, Darzacq, Xavier, Bertrand, Edouard, Jády, Beáta E., Verheggen, Céline, Kiss, Tamás
Post-transcriptional synthesis of 2′-O-methylated nucleotides and pseudouridines in Sm spliceosomal small nuclear RNAs takes place in the nucleoplasmic Cajal bodies and it is directed by guide RNAs...
Dynamic Sorting of Nuclear Components into Distinct Nucleolar Caps during Transcriptional Inhibition
Shav-Tal, Yaron, Blechman, Janna, Darzacq, Xavier, Montagna, Cristina, Dye, Billy T., Patton, James G., ...
Nucleolar segregation is observed under some physiological conditions of transcriptional arrest. This process can be mimicked by transcriptional arrest after actinomycin D treatment leading to the...
Richard, Patricia, Kiss, Arnold M., Darzacq, Xavier, Kiss, Tamás
Processing from pre-mRNA introns is a widespread mechanism to generate human box C/D and H/ACA snoRNAs. Recent studies revealed that an optimal position relative to the 3′ splice site is important...
Nucleolar Factors Direct the 2′-O-Ribose Methylation and Pseudouridylation of U6 Spliceosomal RNA
Ganot, Philippe, Jády, Beáta E., Bortolin, Marie-Line, Darzacq, Xavier, Kiss, Tamás
The nucleolus has long been known as a functionally highly specialized subnuclear compartment where synthesis, posttranscriptional modification, and processing of cytoplasmic rRNAs take place. In...
Processing of Intron-Encoded Box C/D Small Nucleolar RNAs Lacking a 5′,3′-Terminal Stem Structure
The C and D box-containing (box C/D) small nucleolar RNAs (snoRNAs) function in the nucleolytic processing and 2′-O-methylation of precursor rRNA. In vertebrates, most box C/D snoRNAs are processed...
Darzacq, Xavier, Jády, Beáta E., Verheggen, Céline, Kiss, Arnold M., Bertrand, Edouard, Kiss, Tamás
Cajal (coiled) bodies are conserved subnuclear organelles that are present in the nucleoplasm of both animal and plant cells. Although Cajal bodies were first described nearly 100 years ago, their...
A Cajal body-specific pseudouridylation guide RNA is composed of two box H/ACA snoRNA-like domains
Kiss, Arnold M., Jády, Beáta E., Darzacq, Xavier, Verheggen, Céline, Bertrand, Edouard, Kiss, Tamás
Site-specific post-transcriptional conversion of uridines to pseudouridine in ribosomal RNAs and small nuclear RNAs (snRNAs) is directed by guide RNAs which possess the conserved box H and ACA...
Jády, Beáta E., Darzacq, Xavier, Tucker, Karen E., Matera, A.Gregory, Bertrand, Edouard, Kiss, Tamás
Biogenesis of functional spliceosomal small nuclear RNAs (snRNAs) includes the post-transcriptional covalent modification of numerous internal nucleotides. We have recently demonstrated that...
A common sequence motif determines the Cajal body-specific localization of box H/ACA scaRNAs
Richard, Patricia, Darzacq, Xavier, Bertrand, Edouard, Jády, Beáta E., Verheggen, Céline, Kiss, Tamás
Post-transcriptional synthesis of 2′-O-methylated nucleotides and pseudouridines in Sm spliceosomal small nuclear RNAs takes place in the nucleoplasmic Cajal bodies and it is directed by guide RNAs...
Dynamic Sorting of Nuclear Components into Distinct Nucleolar Caps during Transcriptional Inhibition
Shav-Tal, Yaron, Blechman, Janna, Darzacq, Xavier, Montagna, Cristina, Dye, Billy T., Patton, James G., ...
Nucleolar segregation is observed under some physiological conditions of transcriptional arrest. This process can be mimicked by transcriptional arrest after actinomycin D treatment leading to the...
Richard, Patricia, Kiss, Arnold M., Darzacq, Xavier, Kiss, Tamás
Processing from pre-mRNA introns is a widespread mechanism to generate human box C/D and H/ACA snoRNAs. Recent studies revealed that an optimal position relative to the 3′ splice site is important...
Dynamic association and localization of human H/ACA RNP proteins
Kittur, Nupur, Darzacq, Xavier, Roy, Sujayita, Singer, Robert H., Meier, U. Thomas
Mammalian H/ACA RNPs are essential for ribosome biogenesis, pre-mRNA splicing, and telomere maintenance. To form mature RNA–protein complexes, one H/ACA RNA associates with four core proteins. In...
Gene expression within a dynamic nuclear landscape
Shav-Tal, Yaron, Darzacq, Xavier, Singer, Robert H
Molecular imaging in living cells or organisms now allows us to observe macromolecular assemblies with a time resolution sufficient to address cause-and-effect relationships on specific molecules....
Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells
Darzacq, Xavier, Kittur, Nupur, Roy, Sujayita, Shav-Tal, Yaron, Singer, Robert H., Meier, U. Thomas
Mammalian H/ACA RNPs are essential for ribosome biogenesis, premessenger RNA splicing, and telomere maintenance. These RNPs consist of four core proteins and one RNA, but it is not known how they...