William J. Britt

Accumulation of Virion Tegument and Envelope Proteins in a Stable Cytoplasmic Compartment during Human Cytomegalovirus Replication: Characterization of a Potential Site of Virus Assembly

Sanchez, Veronica, Greis, Kenneth D., Sztul, Elizabeth, Britt, William J.

The assembly of human cytomegalovirus (HCMV) is thought to be similar to that which has been proposed for alphaherpesviruses and involve envelopment of tegumented subviral particles at the nuclear...

Human Cytomegalovirus pp28 (UL99) Localizes to a Cytoplasmic Compartment Which Overlaps the Endoplasmic Reticulum-Golgi-Intermediate Compartment

Sanchez, Veronica, Sztul, Elizabeth, Britt, William J.

Although the assembly of herpesviruses has remained an active area of investigation, considerable controversy continues to surround the cellular location of tegument and envelope acquisition. This...

Human Cytomegalovirus Infection of Caco-2 Cells Occurs at the Basolateral Membrane and Is Differentiation State Dependent

Jarvis, Michael A., Wang, C. Edward, Meyers, Heather L., Smith, Patricia P., Corless, Christopher L., Henderson, Gavin J., ...

Epithelial cells are known to be a major target for human cytomegalovirus (HCMV) infection; however, the analysis of virus-cell interactions has been difficult to approach due to the lack of in vitro...

Experimental Coinfection of Rhesus Macaques with Rhesus Cytomegalovirus and Simian Immunodeficiency Virus: Pathogenesis

Sequar, Getachew, Britt, William J., Lakeman, Fred D., Lockridge, Kristen M., Tarara, Ross P., Canfield, Don R., ...

Human cytomegalovirus (HCMV) possesses low pathogenic potential in an immunocompetent host. In the immunosuppressed host, however, a wide spectrum of infection outcomes, ranging from asymptomatic to...

The Interaction between the Major Capsid Protein and the Smallest Capsid Protein of Human Cytomegalovirus Is Dependent on Two Linear Sequences in the Smallest Capsid Protein

Lai, Lilin, Britt, William J.

The assembly of human cytomegalovirus (HCMV) with recombinant systems has not been accomplished. An understanding of specific interactions between individual capsid proteins could point to unique...

Rapid Genetic Engineering of Human Cytomegalovirus by Using a Lambda Phage Linear Recombination System: Demonstration that pp28 (UL99) Is Essential for Production of Infectious Virus

Britt, William J., Jarvis, Michael, Seo, Jun-Young, Drummond, Derek, Nelson, Jay

A highly efficient lambda phage recombination system previously utilized for studies of bacterial artificial chromosome (BAC)-maintained mouse chromosomal DNA was adapted for the study of the role of...

Phosphorylation of Human Cytomegalovirus Glycoprotein B (gB) at the Acidic Cluster Casein Kinase 2 Site (Ser900) Is Required for Localization of gB to the trans-Golgi Network and Efficient Virus Replication

Jarvis, Michael A., Jones, Thomas R., Drummond, Derek D., Smith, Patsy P., Britt, William J., Nelson, Jay A., ...

Human cytomegalovirus (HCMV) glycoprotein B (gB), encoded by the UL55 open reading frame, is an essential envelope glycoprotein involved in cell attachment and entry. Previously, we identified...

Recombinant Modified Vaccinia Virus Ankara Expressing a Soluble Form of Glycoprotein B Causes Durable Immunity and Neutralizing Antibodies against Multiple Strains of Human Cytomegalovirus

Wang, Zhongde, La Rosa, Corinna, Maas, Rebecca, Ly, Heang, Brewer, John, Mekhoubad, Shahram, ...

Human cytomegalovirus (CMV) is a viral pathogen that infects both genders, who remain asymptomatic unless they receive immunosuppressive drugs or acquire infections that cause reactivation of latent...

Postattachment Events Associated with Viral Entry Are Necessary for Induction of Interferon-Stimulated Genes by Human Cytomegalovirus

Netterwald, James R., Jones, Thomas R., Britt, William J., Yang, Shao-Jun, McCrone, Ian P., Zhu, Hua

Utilizing a human cytomegalovirus-specific fusion inhibitor and an antiglycoprotein H antibody, we studied the role of virion fusion in interferon-stimulated gene (ISG) induction. Our results...

Three-Dimensional Localization of the Smallest Capsid Protein in the Human Cytomegalovirus Capsid

Yu, Xuekui, Shah, Sanket, Atanasov, Ivo, Lo, Pierrette, Liu, Fenyong, Britt, William J., ...

The smallest capsid proteins (SCPs) of the human herpesviruses differ substantially in size and sequence and are thought to impart some unique aspects of infection to their respective viruses. We...

Antigenic Domain 1 Is Required for Oligomerization of Human Cytomegalovirus Glycoprotein B

Britt, William J., Jarvis, Michael A., Drummond, Derek D., Mach, Michael

Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic...

Human Cytomegalovirus UL97 Kinase Is Required for the Normal Intranuclear Distribution of pp65 and Virion Morphogenesis

Prichard, Mark N., Britt, William J., Daily, Shannon L., Hartline, Caroll B., Kern, Earl R.

Recombinant human cytomegaloviruses that do not express UL97 kinase activity exhibit a distinctive plaque morphology characterized by the formation of highly refractile bodies late in infection....

Human Cytomegalovirus Infection Elicits a Glycoprotein M (gM)/gN-Specific Virus-Neutralizing Antibody Response

Shimamura, Masako, Mach, Michael, Britt, William J.

Human cytomegalovirus (HCMV) is a ubiquitous human pathogen that infects 40 to 90% of adult human populations. HCMV infections are often asymptomatic in healthy individuals but can cause severe organ...

Sequence Requirements for Localization of Human Cytomegalovirus Tegument Protein pp28 to the Virus Assembly Compartment and for Assembly of Infectious Virus

Seo, Jun-Young, Britt, William J.

The human cytomegalovirus UL99 open reading frame encodes a 190-amino-acid (aa) tegument protein, pp28, that is myristoylated and phosphorylated. pp28 is essential for assembly of infectious virus,...

Human Cytomegalovirus Attenuates Interleukin-1β and Tumor Necrosis Factor Alpha Proinflammatory Signaling by Inhibition of NF-κB Activation

Jarvis, Michael A., Borton, Jamie A., Keech, Amy M., Wong, John, Britt, William J., Magun, Bruce E., ...

Viral infection is associated with a vigorous inflammatory response characterized by cellular infiltration and release of the proinflammatory cytokines interleukin-1 (IL-1) and tumor necrosis factor...

Accumulation of Virion Tegument and Envelope Proteins in a Stable Cytoplasmic Compartment during Human Cytomegalovirus Replication: Characterization of a Potential Site of Virus Assembly

Sanchez, Veronica, Greis, Kenneth D., Sztul, Elizabeth, Britt, William J.

The assembly of human cytomegalovirus (HCMV) is thought to be similar to that which has been proposed for alphaherpesviruses and involve envelopment of tegumented subviral particles at the nuclear...

Human Cytomegalovirus pp28 (UL99) Localizes to a Cytoplasmic Compartment Which Overlaps the Endoplasmic Reticulum-Golgi-Intermediate Compartment

Sanchez, Veronica, Sztul, Elizabeth, Britt, William J.

Although the assembly of herpesviruses has remained an active area of investigation, considerable controversy continues to surround the cellular location of tegument and envelope acquisition. This...

Human Cytomegalovirus Infection of Caco-2 Cells Occurs at the Basolateral Membrane and Is Differentiation State Dependent

Jarvis, Michael A., Wang, C. Edward, Meyers, Heather L., Smith, Patricia P., Corless, Christopher L., Henderson, Gavin J., ...

Epithelial cells are known to be a major target for human cytomegalovirus (HCMV) infection; however, the analysis of virus-cell interactions has been difficult to approach due to the lack of in vitro...

Experimental Coinfection of Rhesus Macaques with Rhesus Cytomegalovirus and Simian Immunodeficiency Virus: Pathogenesis

Sequar, Getachew, Britt, William J., Lakeman, Fred D., Lockridge, Kristen M., Tarara, Ross P., Canfield, Don R., ...

Human cytomegalovirus (HCMV) possesses low pathogenic potential in an immunocompetent host. In the immunosuppressed host, however, a wide spectrum of infection outcomes, ranging from asymptomatic to...

The Interaction between the Major Capsid Protein and the Smallest Capsid Protein of Human Cytomegalovirus Is Dependent on Two Linear Sequences in the Smallest Capsid Protein

Lai, Lilin, Britt, William J.

The assembly of human cytomegalovirus (HCMV) with recombinant systems has not been accomplished. An understanding of specific interactions between individual capsid proteins could point to unique...

Rapid Genetic Engineering of Human Cytomegalovirus by Using a Lambda Phage Linear Recombination System: Demonstration that pp28 (UL99) Is Essential for Production of Infectious Virus

Britt, William J., Jarvis, Michael, Seo, Jun-Young, Drummond, Derek, Nelson, Jay

A highly efficient lambda phage recombination system previously utilized for studies of bacterial artificial chromosome (BAC)-maintained mouse chromosomal DNA was adapted for the study of the role of...

Phosphorylation of Human Cytomegalovirus Glycoprotein B (gB) at the Acidic Cluster Casein Kinase 2 Site (Ser900) Is Required for Localization of gB to the trans-Golgi Network and Efficient Virus Replication

Jarvis, Michael A., Jones, Thomas R., Drummond, Derek D., Smith, Patsy P., Britt, William J., Nelson, Jay A., ...

Human cytomegalovirus (HCMV) glycoprotein B (gB), encoded by the UL55 open reading frame, is an essential envelope glycoprotein involved in cell attachment and entry. Previously, we identified...

Recombinant Modified Vaccinia Virus Ankara Expressing a Soluble Form of Glycoprotein B Causes Durable Immunity and Neutralizing Antibodies against Multiple Strains of Human Cytomegalovirus

Wang, Zhongde, La Rosa, Corinna, Maas, Rebecca, Ly, Heang, Brewer, John, Mekhoubad, Shahram, ...

Human cytomegalovirus (CMV) is a viral pathogen that infects both genders, who remain asymptomatic unless they receive immunosuppressive drugs or acquire infections that cause reactivation of latent...

Postattachment Events Associated with Viral Entry Are Necessary for Induction of Interferon-Stimulated Genes by Human Cytomegalovirus

Netterwald, James R., Jones, Thomas R., Britt, William J., Yang, Shao-Jun, McCrone, Ian P., Zhu, Hua

Utilizing a human cytomegalovirus-specific fusion inhibitor and an antiglycoprotein H antibody, we studied the role of virion fusion in interferon-stimulated gene (ISG) induction. Our results...

Three-Dimensional Localization of the Smallest Capsid Protein in the Human Cytomegalovirus Capsid

Yu, Xuekui, Shah, Sanket, Atanasov, Ivo, Lo, Pierrette, Liu, Fenyong, Britt, William J., ...

The smallest capsid proteins (SCPs) of the human herpesviruses differ substantially in size and sequence and are thought to impart some unique aspects of infection to their respective viruses. We...

Antigenic Domain 1 Is Required for Oligomerization of Human Cytomegalovirus Glycoprotein B

Britt, William J., Jarvis, Michael A., Drummond, Derek D., Mach, Michael

Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic...

Human Cytomegalovirus UL97 Kinase Is Required for the Normal Intranuclear Distribution of pp65 and Virion Morphogenesis

Prichard, Mark N., Britt, William J., Daily, Shannon L., Hartline, Caroll B., Kern, Earl R.

Recombinant human cytomegaloviruses that do not express UL97 kinase activity exhibit a distinctive plaque morphology characterized by the formation of highly refractile bodies late in infection....

Human Cytomegalovirus Infection Elicits a Glycoprotein M (gM)/gN-Specific Virus-Neutralizing Antibody Response

Shimamura, Masako, Mach, Michael, Britt, William J.

Human cytomegalovirus (HCMV) is a ubiquitous human pathogen that infects 40 to 90% of adult human populations. HCMV infections are often asymptomatic in healthy individuals but can cause severe organ...

Sequence Requirements for Localization of Human Cytomegalovirus Tegument Protein pp28 to the Virus Assembly Compartment and for Assembly of Infectious Virus

Seo, Jun-Young, Britt, William J.

The human cytomegalovirus UL99 open reading frame encodes a 190-amino-acid (aa) tegument protein, pp28, that is myristoylated and phosphorylated. pp28 is essential for assembly of infectious virus,...

Human Cytomegalovirus Attenuates Interleukin-1β and Tumor Necrosis Factor Alpha Proinflammatory Signaling by Inhibition of NF-κB Activation

Jarvis, Michael A., Borton, Jamie A., Keech, Amy M., Wong, John, Britt, William J., Magun, Bruce E., ...

Viral infection is associated with a vigorous inflammatory response characterized by cellular infiltration and release of the proinflammatory cytokines interleukin-1 (IL-1) and tumor necrosis factor...

Cytoplasmic Envelopment of Human Cytomegalovirus Requires the Postlocalization Function of Tegument Protein pp28 within the Assembly Compartment▿

Seo, Jun-Young, Britt, William J.

The assembly of herpesvirus remains incompletely defined due to the structural complexity of these viruses. Although the assembly of the capsid of these large DNA viruses is well studied and...

The Cytoplasmic Tail of Glycoprotein M (gpUL100) Expresses Trafficking Signals Required for Human Cytomegalovirus Assembly and Replication▿

Krzyzaniak, Magdalena, Mach, Michael, Britt, William J.

The virion envelope of human cytomegalovirus (HCMV) is complex and consists of an incompletely defined number of glycoproteins. The gM/gN protein complex is the most abundant protein component of the...

Altered development of the brain after focal herpesvirus infection of the central nervous system

Koontz, Thad, Bralic, Marina, Tomac, Jelena, Pernjak-Pugel, Ester, Bantug, Glen, Jonjic, Stipan, ...

Human cytomegalovirus infection of the developing central nervous system (CNS) is a major cause of neurological damage in newborn infants and children. To investigate the pathogenesis of this human...

Multimerization of Tegument Protein pp28 within the Assembly Compartment Is Required for Cytoplasmic Envelopment of Human Cytomegalovirus▿

Seo, Jun-Young, Britt, William J.

Human cytomegalovirus (HCMV) UL99-encoded pp28 is an essential tegument protein required for envelopment and production of infectious virus. Nonenveloped virions accumulate in the cytoplasm of cells...