Lubelski, Jacek, Merkerk, Ronald Van, Konings, Wil N., Driessen, Arnold J.M.
LmrCD is a lactococcal, heterodimeric multidrug transporter, which belongs to the ABC superfamily. It consists of two half-transporters, LmrC and LmrD, that are necessary and sufficient for drug...
Plantinga, Titia H., Does, Chris Van Der, Tomkiewicz, Danuta, Keulen, Geertje Van, Konings, Wil N., Driessen, Arnold J.M.
Binding-protein-dependent secondary transporters make up a unique transport protein family. They use a solute-binding protein in proton-motive-force-driven transport. Only a few systems have been...
Poolman, Bert, Doeven, Mark K., Geertsma, Eric R., Biemans‐Oldehinkel, Esther, Konings, Wil N., Rees, Douglas C.
ATP‐binding cassette (ABC) transporters are vital to any living system and are involved in the translocation of a wide variety of substances, from ions and nutrients to high molecular weight...
Two homologous oligopeptide binding protein genes (oppA) in Lactococcus lactis MG1363 (2004)
Konings, Wil N., Kok, Jan, Hellendoorn, Michiel, Lanfermeijer, Frank C., Sanz, Yolanda, Poolman, Bert
In previous studies, it has been shown that inactivation of opp or even oppA abolishes the capacity of Lactococcus lactis to utilize oligopeptides. We now show that the opp operon has been duplicated...
Mazurkiewicz, Piotr, Driessen, Arnold J.M., Konings, Wil N.
LmrP, a proton/multidrug antiporter of Lactococcus lactis, transports a variety of cationic substrates. Previously, two membrane-embedded acidic residues, Asp142 and Glu327, have been reported to be...
Lubelski, Jacek, Mazurkiewicz, Piotr, Merkerk, Ronald Van, Konings, Wil N., Driessen, Arnold J.M.
Multidrug resistance (MDR)-type transporters mediate the active extrusion of structurally and functionally dissimilar compounds from the cells, thereby rendering cells resistant to a range of drugs....
Facilitated Drug Influx by an Energy-uncoupled Secondary Multidrug Transporter (2004)
Mazurkiewicz, Piotr, Poelarends, Gerrit J., Driessen, Arnold J.M., Konings, Wil N.
The majority of bacterial multidrug resistance transporters belong to the class of secondary transporters. LmrP is a proton/drug antiporter of Lactococcus lactis that extrudes positively charged...
Functional characterization of the Escherichia coli K-12 yiaMNO transport protein genes (2004)
Plantinga, Titia H., Does, Chris Van Der, Badia, Josefa, Aguilar, Juan, Konings, Wil N., Driessen, Arnold J.M.
The yiaMNO genes of Escherichia coli K-12 encode a binding protein-dependent secondary, or tri-partite ATP-independent periplasmic (TRAP), transporter. Since only a few members of this family have...
Insights Into ABC Transport in Archaea (2004)
Albers, Sonja-Verena, Koning, Sonja M., Konings, Wil N., Driessen, Arnold J.M.
In archaea, ATP-binding cassette (ABC) transporters play a crucial role in substrate uptake, export, and osmoregulation. Archaeal substrate-binding-protein-dependent ABC transporters are equipped...
Transporters involved in uptake of di- and tricarboxylates in Bacillus subtilis (2003)
Krom, Bastiaan P., Warner, Jessica B., Konings, Wil N., Lolkema, Juke S.
Di- and tricarboxylates found as intermediates in the tricarboxylic acid cycle can be utilized by many bacteria and serve as carbon and energy source under aerobic and anaerobic conditions. A...
Kamp, Mart Van De, Berg, Marco Van Den, Sjollema, Klaas, Bovenberg, Roel A.L., Veenhuis, Marten, ...
Penicillin biosynthesis by Penicillium chrysogenum is a compartmentalized process. The first catalytic step is mediated by δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACV synthetase), a...
Trip, Hein, Evers, Melchior E., Konings, Wil N., Driessen, Arnold J.M.
The gene encoding the amino acid permease ArlP (Aromatic and leucine Permease) was isolated from the filamentous fungus Penicillium chrysogenum after PCR using degenerated oligonucleotides based on...
Assessment of the microbody luminal pH in the filamentous fungus Penicillium chrysogenum (2002)
Breeuwer, Pieter, Abee, Tjakko, Konings, Wil N., Driessen, Arnold J.M.
The enzymes of the penicillin biosynthetic pathway in Penicillium chrysogenum are located in different subcellular compartments. Consequently, penicillin pathway precursors and the biologically...
Sugar transport in (hyper)thermophilic archaea (2002)
Koning, Sonja M., Albers, Sonja-Verena, Konings, Wil N., Driessen, Arnold J.M.
Hyperthermophilic archaea show important metabolic adaptations for growth on carbohydrates under hostile conditions. For carbohydrate uptake so far only ABC-type transporters have been described that...
Konings, Wil N., Albers, Sonja-Verena, Koning, Sonja, Driessen, Arnold J.M.
The cytoplasmic membrane of bacteria and archaea determine to a large extent the composition of the cytoplasm. Since the ion and in particular the proton and/or the sodium ion electrochemical...
Regulation of maltose transport in Saccharomyces cerevisiae (2001)
Konings, Wil N., Brondijk, T. Harma C., Poolman, Bert
Solute transport in Saccharomyces cerevisiae can be regulated through mechanisms such as trans-inhibition and/or catabolite inactivation by nitrogen or carbon sources. Studies in hybrid membranes of...
Konings, Wil N., Bolotin, Alexander, Renault, Pierre, Lanfermeijer, Frank C., Sanz, Yolanda, Poolman, Bert
The genes encoding a binding-protein-dependent ABC transporter for dipeptides (Dpp) were identified in Lactococcus lactis subsp. cremoris MG1363. Two (dppA and dppP) of the six ORFs (dppAdppPBCDF)...
Elferink, Marieke G.L., Albers, Sonja-V., Konings, Wil N., Driessen, Arnold J.M.
The extreme thermoacidophilic archaeon Sulfolobus solfataricus grows optimally at 80°C and pH 3 and uses a variety of sugars as sole carbon and energy source. Glucose transport in this organism is...
Koning, Sonja M., Elferink, Marieke G.L., Konings, Wil N., Driessen, Arnold J.M.
The hyperthermophilic archaeon Pyrococcus furiosus can utilize different β-glucosides, like cellobiose and laminarin. Cellobiose uptake occurs with high affinity (Km = 175 nM) and involves an...
Bioenergetics and solute uptake under extreme conditions (2001)
Albers, Sonja-V., Driessen, Arnold J.M., Konings, Wil N.
The ion and particularly the proton and sodium ion permeabilities of cytoplasmic membranes play crucial roles in the bioenergetics of microorganisms. The proton and sodium permeabilities of membranes...
Glutamate transporters combine transporter- and channel-like features (2001)
Slotboom, Dirk Jan, Konings, Wil N., Lolkema, Juke S.
Glutamate transporters in the mammalian central nervous system have a unique position among secondary transport proteins as they exhibit glutamate-gated chloride-channel activity in addition to...
The structure of glutamate transporters shows channel-like features (2001)
Slotboom, Dirk Jan, Konings, Wil N., Lolkema, Juke S.
Neuronal and glial glutamate transporters remove the excitatory neurotransmitter glutamate from the synaptic cleft and thus prevent neurotoxicity. The proteins belong to a large family of secondary...
Slotboom, Dirk Jan, Konings, Wil N., Lolkema, Juke S.
The carboxyl-terminal membrane-spanning segment 8 of the glutamate transporter GltT of Bacillus stearothermophilus was studied by cysteine-scanning mutagenesis. 21 single cysteine mutants were...
Konings, Wil N., Lanfermeijer, Frank C., Sanz, Yolanda, Poolman, Bert
The gene (dppA) encoding the binding protein of the di-tripeptide ABC transporter of Lactococcus lactis (DppA) was cloned under the control of the nisin promoter. Amplified expression (≈200-fold...
Lactic acid bacteria: the bugs of the new millennium (2000)
Kuipers, Oscar P., Kok, Jan, Konings, Wil N., Poolman, Bert
Lactic acid bacteria (LABs) are widely used in the manufacturing of fermented food and are among the best-studied microorganisms. Detailed knowledge of a number of physiological traits has opened new...
Konings, Wil N., Detmers, Frank J.M., Lanfermeijer, Frank C., Poolman, Bert
Lactococcus lactis degrades exogenous proteins such as β-casein to peptides of 4–30 amino acids, and uses these as nitrogen sources. The binding protein or receptor (OppALl) of the oligopeptide...
Poolman, Bert, Konings, Wil N., Fang, Gang
The peptide transport protein DtpT of Lactococcus lactis was purified and reconstituted into detergent-destabilized liposomes. The kinetics and substrate specificity of the transporter in the...
Lanfermeijer, Frank C., Kunji, Edmund R.S., Picon, Antonia, Konings, Wil N., Poolman, Bert
The kinetic properties of wild-type and mutant oligopeptide binding proteins of Lactococcus lactis were determined. To observe the properties of the mutant proteins in vivo, the oppA gene was deleted...
Tampé, Robert, Jack, Ralph W., Abele, Rupert, Lanfermeijer, Frank C., Detmers, Frank J.M., Konings, Wil N., ...
The oligopeptide transport system (Opp) of Lactococcus lactis has the unique capacity to mediate the transport of peptides from 4 up to at least 18 residues. The substrate specificity of this binding...
Lolkema, Juke S., Konings, Wil N., Brisson, Alain, Boekema, Egbert J., Ubbink-Kok, Trees
The V-type Na+-ATPase of the thermophilic, anaerobic bacterium Caloramator fervidus was purified to homogeneity. The subunit compositions of the catalytic V1 and membrane-embedded V0 parts were...
Moll, Gert N., Brul, Stanley, Konings, Wil N., Driessen, Arnold J.M.
Ac-MB21-NH2 (Ac-FASLLGKALKALAKQ-NH2) and dermaseptin S3(1-16)-NH2 (ALWKNMLKGIGKLAGK-NH2) are cationic amphipathic peptides with antimicrobial activity against a broad spectrum of microorganisms...
Diversity of transport mechanisms: common structural principles (2000)
Driessen, Arnold J.M., Rosen, Barry P., Konings, Wil N.
Traditionally, prokaryotic solute transport systems are classified into major groups based on the energetic requirement of the transport process. These include the secondary transporters that are...
Sulfur Regulation of the Sulfate Transporter Genes sutA and sutB in Penicillium chrysogenum (2000)
Kamp, Mart Van De, Schuurs, Theo A., Vos, Arnold, Konings, Wil N., Driessen, Arnold J.M.
Penicillium chrysogenum uses sulfate as a source of sulfur for the biosynthesis of penicillin. Sulfate uptake and the mRNA levels of the sulfate transporter-encoding sutB and sutA genes are all...
Krom, Bastiaan P., Warner, Jessica B., Konings, Wil N., Lolkema, Juke S.
Citrate uptake in Bacillus subtilis is stimulated by a wide range of divalent metal ions. The metal ions were separated into two groups based on the expression pattern of the uptake system. The two...
Catabolite Repression and Induction of the Mg2+-Citrate Transporter CitM of Bacillus subtilis (2000)
Warner, Jessica B., Krom, Bastiaan P., Magni, Christian, Konings, Wil N., Lolkema, Juke S.
In Bacillus subtilis the citM gene encodes the Mg2+-citrate transporter. A target site for carbon catabolite repression (cre site) is located upstream of citM. Fusions of the citM promoter region,...
Konings, Wil N., Poolman, Bert, Rest, Michel Van Der, Fristedt, Ulrika, Persson, Bengt L.
The proton-coupled Pho84 phosphate permease of Saccharomyces cerevisiae, overexpressed as a histidine-tagged chimera in Escherichia coli, was detergent-solubilized, purified, and reconstituted into...
Konings, Wil N., Picon, Antonia, Lanfermeijer, Frank C., Poolman, Bert
The oligopeptide transport system (Opp) of Lactococcus lactis belongs to the class of binding protein-dependent ABC-transporters. This system has the unique capacity to mediate the uptake of peptides...
Poolman, Bert, Veen, Hendrik W. Van, Putman, Monique, Konings, Wil N.
The histidine-tagged secondary multidrug transporter LmrP was overexpressed in Lactococcus lactis, using a novel protein expression system for cytotoxic proteins based on the tightly regulated,...
Poolman, Bert, Hagting, Anja, Lanfermeijer, Frank, Friesen, Robert, Fang, Gang, Konings, Wil N.
The di-tripeptide transport system (DtpT) of Lactococcus lactis was purified to apparent homogeneity by pre-extraction of crude membrane vesicles with octaethylene glycol monodecyl ether (C10E8),...
Driessen, Arnold J.M., Costa, Milton S. Da, Konings, Wil N.
Bacillus subtilis was grown at its growth temperature limits and at various temperatures in between the lower and upper growth temperature boundary. Liposomes were made of the extracted membrane...
A unique short signal sequence in membrane-anchored proteins of Archaea (1999)
Albers, Sonja-Verena, Konings, Wil N., Driessen, Arnold J.M.
Kamp, Mart Van De, Pizzinini, Enrica, Vos, Arnold, Schuurs, Theo A., Newbert, Roger W., ...
In industrial fermentations, Penicillium chrysogenum uses sulfate as the source of sulfur for the biosynthesis of penicillin. By a PCR-based approach, two genes, sutA and sutB, whose encoded products...
Moll, Gert N., Akker, Emile Van Den, Hauge, Håvard H., Nissen-Meyer, Jon, Nes, Ingolf F., Konings, Wil N., ...
Plantaricin EF and JK are both two-peptide bacteriocins produced by Lactobacillus plantarum C11. The mechanism of plantaricin EF and JK action was studied on L. plantarum 965 cells. Both plantaricins...
Albers, Sonja-V., Elferink, Marieke G.L., Charlebois, Robert L., Sensen, Christoph W., Driessen, Arnold J.M., Konings, Wil N.
The archaeon Sulfolobus solfataricus grows optimally at 80°C and pH 2.5 to 3.5 on carbon sources such as yeast extracts, tryptone, and various sugars. Cells rapidly accumulate glucose. This...
Driessen, Arnold J.M., Grant, William D., Konings, Wil N.
The influence of pH and the salt concentration on the proton and sodium ion permeability of liposomes formed from lipids of the halophile Halobacterium salinarum and the haloalkaliphile Halorubrum...
Bacteriocins: mechanism of membrane insertion and pore formation (1999)
Moll, Gert N., Konings, Wil N., Driessen, Arnold J.M.
Lactic acid bacteria produce several types of pore forming peptides. Class I bacteriocins are lantibiotics that contain (methyl)lanthionine residues that may form intramolecular thioether rings....
Compartmentalization and transport in β-lactam antibiotic biosynthesis by filamentous fungi (1999)
Kamp, Mart Van De, Driessen, Arnold J.M., Konings, Wil N.
A proper description of the biosynthesis of fungal β-lactam antibiotics requires detailed knowledge of the cell biology of the producing organisms. This involves a delineation of the...
Slotboom, Dirk Jan, Sobczak, Iwona, Konings, Wil N., Lolkema, Juke S.
Neuronal and glial glutamate transporters remove the excitatory neurotransmitter glutamate from the synaptic cleft. The proteins belong to a large family of secondary transporters, which includes...
Magni, Christian, Mendoza, Diego De, Konings, Wil N., Lolkema, Juke S.
Measurement of the flux through the citrate fermentation pathway in resting cells of Lactococcus lactis CRL264 grown in a pH-controlled fermentor at different pH values showed that the pathway was...
Lanfermeijer, Frank C., Kunji, Edmund R.S., Detmers, Frank J.M., Poolman, Bert, Konings, Wil N.
To obtain amino acids for growth, Lactococcus lactis uses a proteolytic system to degrade exogenous proteins such as caseins. The extracellular cell wall-attached proteinase PrtP and the oligopeptide...
Bacterial solute uptake and efflux systems (1998)
Poolman, Bert, Lolkema, Juke S., Konings, Wil N.
The recent discovery of binding protein dependent secondary transporters and the ever-growing family of membrane potential generating secondary transporters emphasize the diversity of transport...
Amino acid transport in Lactobacillus helveticus (1998)
Poolman, Bert, Kunji, Edmund R.S., Nakajima, Hadjime, Konings, Wil N.
Amino acid transport in Lactobacillus helveticus was analyzed. Strain specificity of amino acid transport was speculated between L. helveticus NCDO2712 and SBT2171. Glucose energized L. helveticus...
Physiological Response of Lactobacillus plantarum to Salt and Nonelectrolyte Stress (1998)
Steeg, Pieter F. Ter, Tjan, Frans S.B., Glaasker, Erwin, Konings, Wil N., Poolman, Bert
In this report, we compared the effects on the growth of Lactobacillus plantarum of raising the medium molarity by high concentrations of KCl or NaCl and iso-osmotic concentrations of nonionic...
Konings, Wil N., Glaasker, Erwin, Poolman, Bert
The accumulation of quaternary ammonium compounds in Lactobacillus plantarum is mediated via a single transport system with a high affinity for glycine betaine (apparent Km of 18 μM) and carnitine...
Stingl, Kerstin, Vries, Yne De, Pluim, Dick, Brondijk, T. Harma C., Poolman, Bert, ...
The maltose transporter of Saccharomyces cerevisiae is subject to rapid, irreversible inactivation in the presence of glucose. Loss of transport function was paralleled by a decrease in amount of...
Reconstruction of the proteolytic pathway for use of β-casein by Lactococcus lactis (1998)
Bruins, Andries P., Jeronimus-Stratingh, C. Margot, Fang, Gang, Kunji, Edmund R.S., Poolman, Bert, Konings, Wil N.
Amino acid auxotrophous bacteria such as Lactococcus lactis use proteins as a source of amino acids. For this process, they possess a complex proteolytic system to degrade the protein(s) and to...
Hauge, Håvard Hildeng, Mantzilas, Dimitris, Moll, Gert N., Konings, Wil N., Driessen, Arnold J.M., Eijsink, Vincent G.H., ...
Production of bacteriocins by lactic acid bacteria is in some cases regulated by a quorum sensing mechanism that involves a secreted bacteriocin-like peptide pheromone. In the case of Lactobacillus...
Mechanistic Properties of the Two-Component Bacteriocin Lactococcin G (1998)
Moll, Gert, Hildeng-Hauge, Håvard, Nissen-Meyer, Jon, Nes, Ingolf F., Konings, Wil N., Driessen, Arnold J.M.
Lactococcin G is a bacteriocin whose activity depends on the complementary action of two peptides, termed α and β. Biologically active, synthetic lactococcin G was used to study the mode of action...
The Lantibiotic Nisin Induces Transmembrane Movement of a Fluorescent Phospholipid (1998)
Moll, Gert N., Konings, Wil N., Driessen, Arnold J.M.
Nisin is a pore-forming antimicrobial peptide. The capacity of nisin to induce transmembrane movement of a fluorescent phospholipid in lipid vesicles was investigated. Unilamellar phospholipid...
Driessen, Arnold J.M., Zillig, Wolfram, Konings, Wil N.
Picrophilus oshimae is an extremely acidophilic, thermophilic archae on that grows optimally at 60°C and at pH 0.7. It is an obligatory acidophile that does not grow at pH values above 4.0. The...
The essence of being extremophilic: the role of the unique archaeal membrane lipids (1998)
Driessen, Arnold J.M., Konings, Wil N.
In extreme environments, mainly Archaea are encountered. The archaeal cytoplasmic membrane contains unique ether lipids that cannot easily be degraded, are temperature- and mechanically resistant,...
Kunji, Edmund R.S., Hagting, Anja, Nakajima, Hajime, Poolman, Bert, Konings, Wil N.
The gene encoding the di- and tripeptide transport protein (DtpT) of Lactobacillus helveticus (DtpTLH) was cloned with the aid of the inverse PCR technique and used to complement the dipeptide...
Membrane Topology of the Di- and Tripeptide Transport Protein of Lactococcus lactis (1997)
Poolman, Bert, Velde, Joeke V.d., Hagting, Anja, Konings, Wil N.
Transport of hydrophilic di- and tripeptides into Lactococcus lactis is mediated by a proton motive force-driven peptide transport protein (DtpT) that shares similarity with eukaryotic peptide...
Adaptation of Microorganisms and Their Transport Systems to High Temperatures (1997)
Konings, Wil N., Poolman, Bert, Tolner, Berend
Growth of Bacteria and Archaea has been observed at temperatures up to 95 and 110°C, respectively. These thermophiles are adapted to environments of high temperature by changes in the membrane lipid...
Fang, Gang, Streutker, Marion R., Hasemeier, Britta, Knol, Jan, Hagting, Anja, Poolman, Bert, ...
Transport of hydrophilic dipeptides and tripeptides into Lactococcus lactis is mediated by a proton-motive-force-driven peptide-transport protein (DtpT) that shares similarity to eukaryotic peptide...
Mechanisms of multidrug transporters (1997)
Konings, Wil N., Driessen, Arnold J.M., Poolman, Bert, Veen, Hendrik W. Van, Bolhuis, Henk
Drug resistance, mediated by various mechanisms, plays a crucial role in the failure of the drug-based treatment of various infectious diseases. As a result, these infectious diseases re-emerge...