Wieland B. Huttner

Live Imaging at the Onset of Cortical Neurogenesis Reveals Differential Appearance of the Neuronal Phenotype in Apical versus Basal Progenitor Progeny (2008)

Attardo, Alessio, Calegari, Federico, Haubensak, Wulf, Wilsch-Bräuninger, Michaela, Huttner, Wieland B.

The neurons of the mammalian brain are generated by progenitors dividing either at the apical surface of the ventricular zone (neuroepithelial and radial glial cells, collectively referred to as...

On the origin of neurons (2007)

Pulvers, Jeremy N, Schenk, Judith, Arai, Yoko, Fei, Ji-Feng, Saito, Kanako, Huttner, Wieland B

A report on the conference 'Neurogenesis 2007', Tokyo, Japan, 15-16 May 2007.

Focus on Molecules: Prominin-1 (CD133) (2007)

Jaszai, Jozsef, Fargeas, Christine A., Florek, Mareike, Huttner, Wieland B., Corbeil, Denis

Differential expression of Prominin-1 (CD133) and Prominin-2 in major cephalic exocrine glands of adult mice (2007)

Jaszai, Jozsef, Janich, Peggy, Farkas, Lilla M., Fargeas, Christine A., Huttner, Wieland B., Corbeil, Denis

The major cephalic exocrine glands share many morphological and functional features and so can be simultaneously affected in certain autoimmune- and inherited disorders leading to glandular...

Neuronal and glial cell biology (2007)

Craig, Ann Marie, Huttner, Wieland B.

Cell polarity from cell division (2007)

Knust, Elisabeth, Huttner, Wieland B.

Apical-basal polarity of epithelial cells is critical for their symmetric versus asymmetric division and commonly thought to be established in interphase. In a novel type of cell division termed...

Neuronale Stamm- und Vorlaeuferzellen (2007)

Wilsch-Braeuninger, Michaela, Huttner, Wieland B.

Das zentrale Nervensystem von Wirbeltieren entsteht in der Embryonalentwicklung aus einem einschichtigen Epithel, dem Neuroepithel. Waehrend der Neurogenese bildet sich aus diesem einfachen Epithel...

On the origin of neurons (2007)

Pulvers, Jeremy N., Schenk, Judith, Arai, Yoko, Fei, Ji-Feng, Saito, Kanako, Huttner, Wieland B.

Nomenclature of prominin-1 (CD133) splice variants - an update (2007)

Fargeas, Christine A., Huttner, Wieland B., Corbeil, Denis

Prominin-1 (CD133), a pentaspan membrane glycoprotein that constitutes an important cell surface marker of various, either normal or cancerous, stem cell populations is widely used to isolate or...

Midbody and primary cilium of neural progenitors release extracellular membrane particles enriched in the stem cell marker prominin-1 (2007)

Dubreuil, Veronique, Marzesco, Anne-Marie, Corbeil, Denis, Huttner, Wieland B., Wilsch-Brauninger, Michaela

Expansion of the neocortex requires symmetric divisions of neuroepithelial cells, the primary progenitor cells of the developing mammalian central nervous system. Symmetrically dividing...

Prominin-2 is a cholesterol-binding protein associated with apical and basolateral plasmalemmal protrusions in polarized epithelial cells and released into urine (2007)

Florek, Mareike, Bauer, Nicola, Janich, Peggy, Wilsch-Brauninger, Michaela, Fargeas, Christine, Marzesco, Anne-Marie, ...

Prominin-2 is a pentaspan membrane glycoprotein structurally related to the cholesterol-binding protein prominin-1, which is expressed in epithelial and non-epithelial cells. Although prominin-1...

Prominin-1 (CD133): from progenitor cells to human diseases (2006)

Fargeas, Christine A., Fonseca, Ana-Violeta, Huttner, Wieland B., Corbeil, Denis

The Rho-GTPase cdc42 regulates neural progenitor fate at the apical surface (2006)

Cappello, Silvia, Attardo, Alessio, Wu, Xunwei, Iwasato, Takuji, Itohara, Shigeyoshi, Wilsch-Brauninger, Michaela, ...

Stem cell persistence into adulthood requires self-renewal from early developmental stages. In the developing mouse brain, only apical progenitors located at the ventricle are self-renewing, whereas...

Aspm specifically maintains symmetric proliferative divisions of neuroepithelial cells (2006)

Fish, Jennifer L, Kosodo, Yoichi, Enard, Wolfgang, Paabo, Svante, Huttner, Wieland B

The ASPM (abnormal spindle-like microcephaly-associated) protein has previously been implicated in the determination of human cerebral cortical size, but the cell biological basis of this regulation...

Aspm specifically maintains symmetric proliferative divisions of neuroepithelial cells (2006)

Fish, Jennifer L., Kosodo, Yoichi, Enard, Wolfgang, Pääbo, Svante, Huttner, Wieland B.

Neurogenesis in the Central Nervous System (2006)

Dubreuil, Veronique, Farkas, Lilla, Calegari, Federico, Kosodo, Yoichi, Huttner, Wieland B.

Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis (2004)

Haubensak,Wulf, Attardo,Alessio, Denk,Winfried, Huttner,Wieland B.

Neurons of the mammalian CNS are thought to originate from progenitors dividing at the apical surface of the neuroepithelium. Here we use mouse embryos expressing GFP from the Tis21 locus, a gene...

Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis (2004)

Haubensak, Wulf, Attardo, Alessio, Denk, Winfried, Huttner, Wieland B.

Neurons of the mammalian CNS are thought to originate from progenitors dividing at the apical surface of the neuroepithelium. Here we use mouse embryos expressing GFP from the Tis21 locus, a gene...

Asymmetric distribution of the apical plasma membrane during neurogenic divisions of mammalian neuroepithelial cells. (2004)

Kosodo, Yoichi, Roper, Katja, Haubensak, Wulf, Marzesco, Anne-Marie, Corbeil, Denis, Huttner, Wieland B

At the onset of neurogenesis in the mammalian central nervous system, neuroepithelial cells switch from symmetric, proliferative to asymmetric, neurogenic divisions. In analogy to the asymmetric...

Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis. (2004)

Haubensak, Wulf, Attardo, Alessio, Denk, Winfried, Huttner, Wieland B

Neurons of the mammalian CNS are thought to originate from progenitors dividing at the apical surface of the neuroepithelium. Here we use mouse embryos expressing GFP from the Tis21 locus, a gene...

Tyrosine Sulfation (2004)

Corbeil, Denis, Huttner, Wieland B.

The O-sulfation of tyrosine residues of membrane and secretory proteins that transit through the secretory pathway of eukaryotic cells is a ubiquitous posttranslational modification conserved in all...

Identification of novel Prominin-1/CD133 splice variants with alternative C-termini and their expression in epididymis and testis. (2004)

Fargeas, Christine A, Joester, Angret, Missol-Kolka, Ewa, Hellwig, Andrea, Huttner, Wieland B, Corbeil, Denis

Prominin-1/CD133 is a five-membrane-span glycoprotein that is thought to act as an organizer of plasma-membrane protrusions. Here, we report the molecular and cell-biological characterization of four...

Tissue-specific RNA interference in post-implantation mouse embryos using directional electroporation and whole embryo culture. (2004)

Calegari, Federico, Marzesco, Anne-Marie, Kittler, Ralf, Buchholz, Frank, Huttner, Wieland B

Abstract In mammals, embryonic development is more difficult to analyze than in non-mammalian species because this development occurs in utero. Interestingly, whole embryo culture allows the normal...

Prominin-1/CD133, a neural and hematopoietic stem cell marker, is expressed in adult human differentiated cells and certain types of kidney cancer. (2004)

Florek, Mareike, Haase, Michael, Marzesco, Anne-Marie, Freund, Daniel, Ehninger, Gerhard, Huttner, Wieland B, ...

Human prominin-1/CD133 has been reported to be expressed in neural and hematopoietic stem/progenitor cells and in embryonic, but not adult, epithelia. This lack of detection of human prominin-1, as...

Asymmetric cell division during neurogenesis in Drosophila and vertebrates. (2003)

Wodarz, Andreas, Huttner, Wieland B

The majority of cells that build the nervous system of animals are generated early in embryonic development in a process called neurogenesis. Although the vertebrate nervous system is much more...

AC133 antigen, CD133, prominin-1, prominin-2, etc.: prominin family gene products in need of a rational nomenclature. (2003)

Fargeas, Christine A, Corbeil, Denis, Huttner, Wieland B

An inhibition of cyclin-dependent kinases that lengthens, but does not arrest, neuroepithelial cell cycle induces premature neurogenesis. (2003)

Calegari, Federico, Huttner, Wieland B

The G1 phase of the cell cycle of neuroepithelial cells, the progenitors of all neurons of the mammalian central nervous system, has been known to lengthen concomitantly with the onset and...

Essential role of endophilin A in synaptic vesicle budding at the Drosophila neuromuscular junction (2002)

Guichet, Antoine, Wucherpfennig, Tanja, Dudu, Veronica, Etter, Sylvain, Wilsch-Bräuniger, Michaela, Hellwig, Andrea, ...

We characterized Drosophila endophilin A (D-endoA), and generated and analysed D-endoA mutants. Like its mammalian homologue, D-endoA exhibits lysophosphatidic acid acyl transferase activity and...

Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA (2002)

Calegari, Federico, Haubensak, Wulf, Yang, Dun, Huttner, Wieland B., Buchholz, Frank

RNA interference (RNAi) using double-stranded RNA has been used for the systematic analysis of gene function in invertebrate organisms. Here we have explored the use of short interfering RNA (siRNA)...

Expression of the antiproliferative gene TIS21 at the onset of neurogenesis identifies single neuroepithelial cells that switch from proliferative to neuron-generating division

Iacopetti, Paola, Michelini, Monica, Stuckmann, Ingo, Oback, Björn, Aaku-Saraste, Eeva, Huttner, Wieland B.

At the onset of mammalian neurogenesis, neuroepithelial (NE) cells switch from proliferative to neuron-generating divisions. Understanding the molecular basis of this switch requires the ability to...

Existence of distinct tyrosylprotein sulfotransferase genes: Molecular characterization of tyrosylprotein sulfotransferase-2

Beisswanger, Roland, Corbeil, Denis, Vannier, Christian, Thiele, Christoph, Dohrmann, Ulrike, Kellner, Roland, ...

Tyrosylprotein sulfotransferase (TPST) is a 54- to 50-kDa integral membrane glycoprotein of the trans-Golgi network found in essentially all tissues investigated, catalyzing the tyrosine O-sulfation...

Prominin, a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells

Weigmann, Anja, Corbeil, Denis, Hellwig, Andrea, Huttner, Wieland B.

Using a new mAb raised against the mouse neuroepithelium, we have identified and cDNA-cloned prominin, an 858-amino acid-containing, 115-kDa glycoprotein. Prominin is a novel plasma membrane protein...

Two overlapping reading frames in a single exon encode interacting proteins—a novel way of gene usage

Klemke, Martin, Kehlenbach, Ralph H., Huttner, Wieland B.

The >1 kb XL-exon of the rat XLαs/Gαs gene encodes the 37 kDa XL-domain, the N-terminal half of the 78 kDa neuroendocrine-specific G-protein α-subunit XLαs. Here, we describe a novel feature...

Essential role of endophilin A in synaptic vesicle budding at the Drosophila neuromuscular junction

Guichet, Antoine, Wucherpfennig, Tanja, Dudu, Veronica, Etter, Sylvain, Wilsch-Bräuniger, Michaela, Hellwig, Andrea, ...

We characterized Drosophila endophilin A (D-endoA), and generated and analysed D-endoA mutants. Like its mammalian homologue, D-endoA exhibits lysophosphatidic acid acyl transferase activity and...

Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA

Calegari, Federico, Haubensak, Wulf, Yang, Dun, Huttner, Wieland B., Buchholz, Frank

RNA interference (RNAi) using double-stranded RNA has been used for the systematic analysis of gene function in invertebrate organisms. Here we have explored the use of short interfering RNA (siRNA)...

Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: A major site of neurogenesis

Haubensak, Wulf, Attardo, Alessio, Denk, Winfried, Huttner, Wieland B.

Neurons of the mammalian CNS are thought to originate from progenitors dividing at the apical surface of the neuroepithelium. Here we use mouse embryos expressing GFP from the Tis21 locus, a gene...

Asymmetric distribution of the apical plasma membrane during neurogenic divisions of mammalian neuroepithelial cells

Kosodo, Yoichi, Röper, Katja, Haubensak, Wulf, Marzesco, Anne-Marie, Corbeil, Denis, Huttner, Wieland B

At the onset of neurogenesis in the mammalian central nervous system, neuroepithelial cells switch from symmetric, proliferative to asymmetric, neurogenic divisions. In analogy to the asymmetric...

Stimulation by 6-N,2′-O-dibutyryladenosine 3′:5′-cyclic monophosphate and glucocorticoids of phosphoenolpyruvate carboxykinase in the isolated perfused rat liver (Short Communication)

Huttner, Wieland B., Krone, Wilhelm, Seitz, Hans J., Tarnowski, Wolfgang

Dibutyryl cyclic AMP stimulated the activity of phosphoenolpyruvate carboxykinase in perfused livers of rats, fed on a low-protein diet, linearly over a 6h period. The enzyme activity was also...

Expression of the antiproliferative gene TIS21 at the onset of neurogenesis identifies single neuroepithelial cells that switch from proliferative to neuron-generating division

Iacopetti, Paola, Michelini, Monica, Stuckmann, Ingo, Oback, Björn, Aaku-Saraste, Eeva, Huttner, Wieland B.

At the onset of mammalian neurogenesis, neuroepithelial (NE) cells switch from proliferative to neuron-generating divisions. Understanding the molecular basis of this switch requires the ability to...

Existence of distinct tyrosylprotein sulfotransferase genes: Molecular characterization of tyrosylprotein sulfotransferase-2

Beisswanger, Roland, Corbeil, Denis, Vannier, Christian, Thiele, Christoph, Dohrmann, Ulrike, Kellner, Roland, ...

Tyrosylprotein sulfotransferase (TPST) is a 54- to 50-kDa integral membrane glycoprotein of the trans-Golgi network found in essentially all tissues investigated, catalyzing the tyrosine O-sulfation...

Prominin, a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells

Weigmann, Anja, Corbeil, Denis, Hellwig, Andrea, Huttner, Wieland B.

Using a new mAb raised against the mouse neuroepithelium, we have identified and cDNA-cloned prominin, an 858-amino acid-containing, 115-kDa glycoprotein. Prominin is a novel plasma membrane protein...

Two overlapping reading frames in a single exon encode interacting proteins—a novel way of gene usage

Klemke, Martin, Kehlenbach, Ralph H., Huttner, Wieland B.

The >1 kb XL-exon of the rat XLαs/Gαs gene encodes the 37 kDa XL-domain, the N-terminal half of the 78 kDa neuroendocrine-specific G-protein α-subunit XLαs. Here, we describe a novel feature...

Essential role of endophilin A in synaptic vesicle budding at the Drosophila neuromuscular junction

Guichet, Antoine, Wucherpfennig, Tanja, Dudu, Veronica, Etter, Sylvain, Wilsch-Bräuniger, Michaela, Hellwig, Andrea, ...

We characterized Drosophila endophilin A (D-endoA), and generated and analysed D-endoA mutants. Like its mammalian homologue, D-endoA exhibits lysophosphatidic acid acyl transferase activity and...

Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA

Calegari, Federico, Haubensak, Wulf, Yang, Dun, Huttner, Wieland B., Buchholz, Frank

RNA interference (RNAi) using double-stranded RNA has been used for the systematic analysis of gene function in invertebrate organisms. Here we have explored the use of short interfering RNA (siRNA)...

Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: A major site of neurogenesis

Haubensak, Wulf, Attardo, Alessio, Denk, Winfried, Huttner, Wieland B.

Neurons of the mammalian CNS are thought to originate from progenitors dividing at the apical surface of the neuroepithelium. Here we use mouse embryos expressing GFP from the Tis21 locus, a gene...

Asymmetric distribution of the apical plasma membrane during neurogenic divisions of mammalian neuroepithelial cells

Kosodo, Yoichi, Röper, Katja, Haubensak, Wulf, Marzesco, Anne-Marie, Corbeil, Denis, Huttner, Wieland B

At the onset of neurogenesis in the mammalian central nervous system, neuroepithelial cells switch from symmetric, proliferative to asymmetric, neurogenic divisions. In analogy to the asymmetric...

Stimulation by 6-N,2′-O-dibutyryladenosine 3′:5′-cyclic monophosphate and glucocorticoids of phosphoenolpyruvate carboxykinase in the isolated perfused rat liver (Short Communication)

Huttner, Wieland B., Krone, Wilhelm, Seitz, Hans J., Tarnowski, Wolfgang

Dibutyryl cyclic AMP stimulated the activity of phosphoenolpyruvate carboxykinase in perfused livers of rats, fed on a low-protein diet, linearly over a 6h period. The enzyme activity was also...

Aspm specifically maintains symmetric proliferative divisions of neuroepithelial cells

Fish, Jennifer L., Kosodo, Yoichi, Enard, Wolfgang, Pääbo, Svante, Huttner, Wieland B.

The ASPM (abnormal spindle-like microcephaly-associated) protein has previously been implicated in the determination of human cerebral cortical size, but the cell biological basis of this regulation...

Midbody and primary cilium of neural progenitors release extracellular membrane particles enriched in the stem cell marker prominin-1

Dubreuil, Véronique, Marzesco, Anne-Marie, Corbeil, Denis, Huttner, Wieland B., Wilsch-Bräuninger, Michaela

Expansion of the neocortex requires symmetric divisions of neuroepithelial cells, the primary progenitor cells of the developing mammalian central nervous system. Symmetrically dividing...

On the origin of neurons

Pulvers, Jeremy N, Schenk, Judith, Arai, Yoko, Fei, Ji-Feng, Saito, Kanako, Huttner, Wieland B

A report on the conference 'Neurogenesis 2007', Tokyo, Japan, 15-16 May 2007.

Essential Role of the Disulfide-bonded Loop of Chromogranin B for Sorting to Secretory Granules Is Revealed by Expression of a Deletion Mutant in the Absence of Endogenous Granin Synthesis

Krömer, Andreas, Glombik, Michael M., Huttner, Wieland B., Gerdes, Hans-Hermann

Sorting of regulated secretory proteins in the TGN to immature secretory granules (ISG) is thought to involve at least two steps: their selective aggregation and their interaction with membrane...

Synaptic-like Microvesicles of Neuroendocrine Cells Originate from a Novel Compartment That Is Continuous with the Plasma Membrane and Devoid of Transferrin Receptor

Schmidt, Anne, Hannah, Matthew J., Huttner, Wieland B.

We have characterized the compartment from which synaptic-like microvesicles (SLMVs), the neuroendocrine counterpart of neuronal synaptic vesicles, originate. For this purpose we have exploited the...

Live Imaging at the Onset of Cortical Neurogenesis Reveals Differential Appearance of the Neuronal Phenotype in Apical versus Basal Progenitor Progeny

Attardo, Alessio, Calegari, Federico, Haubensak, Wulf, Wilsch-Bräuninger, Michaela, Huttner, Wieland B.

The neurons of the mammalian brain are generated by progenitors dividing either at the apical surface of the ventricular zone (neuroepithelial and radial glial cells, collectively referred to as...

Mutant prominin 1 found in patients with macular degeneration disrupts photoreceptor disk morphogenesis in mice

Yang, Zhenglin, Chen, Yali, Lillo, Concepcion, Chien, Jeremy, Yu, Zhengya, Michaelides, Michel, ...

Familial macular degeneration is a clinically and genetically heterogeneous group of disorders characterized by progressive central vision loss. Here we show that an R373C missense mutation in the...