Volker Sievert

X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1) (2005)

Manjasetty, Babu A, Niesen, Frank H, Scheich, Christoph, Roske, Yvette, Goetz, Frank, Behlke, Joachim, ...

Abstract Background Human Aortic Preferentially Expressed Protein-1 (APEG-1) is a novel specific smooth muscle differentiation marker thought to play a role in the growth and differentiation of...

Structural genomics of human proteins – target selection and generation of a public catalogue of expression clones (2005)

Büssow, Konrad, Scheich, Christoph, Sievert, Volker, Harttig, Ulrich, Schultz, Jörg, Simon, Bernd, ...

Abstract Background The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural...

Structural genomics of human proteins – target selection and generation of a public catalogue of expression clones (2005)

Büssow, Konrad, Scheich, Christoph, Sievert, Volker, Harttig, Ulrich, Schultz, Jörg, Simon, Bernd, ...

Background The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural genomics...

X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1). (2005)

Manjasetty, Babu A, Niesen, Frank H, Scheich, Christoph, Roske, Yvette, Goetz, Frank, Behlke, Joachim, ...

BACKGROUND: Human Aortic Preferentially Expressed Protein-1 (APEG-1) is a novel specific smooth muscle differentiation marker thought to play a role in the growth and differentiation of arterial...

The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L (2004)

Soukenik,Michael, Diehl,Anne, Leidert,Martina, Sievert,Volker, Büssow,Konrad, Leitner,Dietmar, ...

The solution structure of the human p47 SEP domain in a construct comprising residues G1-S2-p47(171–270) was determined by NMR spectroscopy. A structure-derived hypothesis about the domains'...

X-ray structure of Fumarylacetoacetate Hydrolase(FAH) family member Homo sapiens FLJ36880 (2004)

Manjasetty,Babu A., Niesen,Frank H., Delbrueck,Heinrich, Goetz,Frank, Sievert,Volker, Buessow,Konrad, ...

The human protein FLJ36880 belongs to the fumarylacetoacetate hydrolase family. The X-ray structure of FLJ36880 has been determined to 2.2 Å resolution employing the semi-automated high-throughput...

A catalog of human cDNA expression clones and its application to structural genomics (2004)

Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, ...

Abstract We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli . A cDNA expression library...

A catalog of human cDNA expression clones and its application to structural genomics (2004)

Büssow,Konrad, Quedenau,Claudia, Sievert,Volker, Tischer,Janett, Scheich,Christoph, Seitz,Harald, ...

We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825...

Fast identification of folded human protein domains expressed in E. colisuitable for structural analysis (2004)

Scheich, Christoph, Leitner, Dietmar, Sievert, Volker, Leidert, Martina, Schlegel, Brigitte, Simon, Bernd, ...

Abstract Background High-throughput protein structure analysis of individual protein domains requires analysis of large numbers of expression clones to identify suitable constructs for structure...

Fast identification of folded human protein domains expressed in E. coli suitable for structural analysis (2004)

Scheich,Christoph, Leitner,Dietmar, Sievert,Volker, Leidert,Martina, Schlegel,Brigitte, Simon,Bernd, ...

Background High-throughput protein structure analysis of individual protein domains requires analysis of large numbers of expression clones to identify suitable constructs for structure...

The solution structure of the SODD BAG domain reveals additional electrostatic interactions in the HSP70 complexes of SODD subfamily BAG domains (2004)

Brockmann,Christoph, Leitner,Dietmar, Labudde,Dirk, Diehl,Annette, Sievert,Volker, Buessow,Konrad, ...

The solution structure of an N-terminally extended construct of the SODD BAG domain was determined by nuclear magnetic resonance spectroscopy. A homology model of the SODD-BAG/HSP70 complex reveals...

The solution structure of the SODD BAG domain reveals additional electrostatic interactions in the HSP70 complexes of SODD subfamily BAG domains (2004)

Brockmann, Christoph, Leitner, Dietmar, Labudde, Dirk, Diehl, Annette, Sievert, Volker, Buessow, Konrad, ...

The solution structure of an N-terminally extended construct of the SODD BAG domain was determined by nuclear magnetic resonance spectroscopy. A homology model of the SODD-BAG/HSP70 complex reveals...

A catalog of human cDNA expression clones and its application to structural genomics (2004)

Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, ...

We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825...

X-ray structure of Fumarylacetoacetate Hydrolase(FAH) family member Homo sapiens FLJ36880 (2004)

Manjasetty, Babu A., Niesen, Frank H., Delbrueck, Heinrich, Goetz, Frank, Sievert, Volker, Buessow, Konrad, ...

The human protein FLJ36880 belongs to the fumarylacetoacetate hydrolase family. The X-ray structure of FLJ36880 has been determined to 2.2 Å resolution employing the semi-automated high-throughput...

Fast identification of folded human protein domains expressed in E. coli suitable for structural analysis (2004)

Scheich, Christoph, Leitner, Dietmar, Sievert, Volker, Leidert, Martina, Schlegel, Brigitte, Simon, Bernd, ...

Background High-throughput protein structure analysis of individual protein domains requires analysis of large numbers of expression clones to identify suitable constructs for structure...

The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L (2004)

Soukenik, Michael, Diehl, Anne, Leidert, Martina, Sievert, Volker, Büssow, Konrad, Leitner, Dietmar, ...

The solution structure of the human p47 SEP domain in a construct comprising residues G1-S2-p47(171–270) was determined by NMR spectroscopy. A structure-derived hypothesis about the domains'...

An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography (2003)

Scheich, Christoph, Sievert, Volker, Büssow, Konrad

Abstract Background Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods...

BioMed Central (2003)

Bmc Bioinformatics, Konrad Bssow, Steve Hoffmann, Volker Sievert

Background: Functional genomics involves the parallel experimentation with large sets of proteins. This requires management of large sets of open reading frames as a prerequisite of the cloning and...

An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography (2003)

Scheich,Christoph, Sievert,Volker, Buessow,Konrad

Background Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express...

An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography (2003)

Scheich, Christoph, Sievert, Volker, Buessow, Konrad

Background Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express...

ORFer – retrieval of protein sequences and open reading frames from GenBank and storage into relational databases or text files (2002)

Büssow, Konrad, Hoffmann, Steve, Sievert, Volker

Abstract Background Functional genomics involves the parallel experimentation with large sets of proteins. This requires management of large sets of open reading frames as a prerequisite of the...

ORFer - retrieval of protein sequences and open reading frames from GenBank and storage into relational databases or text files (2002)

Buessow,Konrad, Hoffmann,Steve, Sievert,Volker

Background Functional genomics involves the parallel experimentation with large sets of proteins. This requires management of large sets of open reading frames as a prerequisite of the cloning and...

ORFer - retrieval of protein sequences and open reading frames from GenBank and storage into relational databases or text files (2002)

Buessow, Konrad, Hoffmann, Steve, Sievert, Volker

Background Functional genomics involves the parallel experimentation with large sets of proteins. This requires management of large sets of open reading frames as a prerequisite of the cloning and...

A catalog of human cDNA expression clones and its application to structural genomics

Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, ...

A systematic approach for identifying human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli is described.

A catalog of human cDNA expression clones and its application to structural genomics

Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, ...

A systematic approach for identifying human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli is described.