Volker A. Erdmann

Selection of RNA Aptamers to the Alzheimer's Disease Amyloid Peptide (2002)

Ylera,Francisco, Lurz,Rudi, Erdmann,Volker A., Fürste,Jens Peter

Alzheimer's disease is correlated with the deposition of amyloid peptides in the brain of the patients. The amyloid is thus a major target in the search for novel diagnostic and therapeutic...

Selection of RNA Aptamers to the Alzheimer's Disease Amyloid Peptide (2002)

Ylera, Francisco, Lurz, Rudi, Erdmann, Volker A., Fürste, Jens Peter

Alzheimer's disease is correlated with the deposition of amyloid peptides in the brain of the patients. The amyloid is thus a major target in the search for novel diagnostic and therapeutic...

Collection of mRNA-like non-coding RNAs (2001)

Volker A. Erdmann, Maciej Szymanski, Abraham Hochberg, Nathan De Groot

In last few years much data has accumulated which shows that in different cells various RNA transcripts are synthesized. They lack protein coding capacity and do not produce mature protein. It seems...

S rRNA Data Bank (1997)

Maciej Szymanski, Thomas Specht, Miroslawa Z. Barciszewska, Jan Barciszewski, Volker A. Erdmann

In this paper we present the updated version of the compilation of 5S rRNA and 5S rDNA nucleotide sequences. It contains 1622 primary structures of 5S rRNAs and 5S rRNA genes from 888 species. These...

The non-coding RNAs as riboregulators

Erdmann, Volker A., Barciszewska, Miroslawa Z., Szymanski, Maciej, Hochberg, Abraham, Groot, Nathan De, Barciszewski, Jan

The non-coding RNAs database (http://biobases.ibch.poznan.pl/ncRNA/) contains currently available data on RNAs, which do not have long open reading frames and act as riboregulators. Non-coding RNAs...

5S Ribosomal RNA Database

Szymanski, Maciej, Barciszewska, Miroslawa Z., Erdmann, Volker A., Barciszewski, Jan

Ribosomal 5S RNA (5S rRNA) is an integral component of the large ribosomal subunit in all known organisms with the exception only of mitochondrial ribosomes of fungi and animals. It is thought to...

Non-coding, mRNA-like RNAs database Y2K

Erdmann, Volker A., Szymanski, Maciej, Hochberg, Abraham, Groot, Nathan De, Barciszewski, Jan

In last few years much data has accumulated on various non-translatable RNA transcripts that are synthesised in different cells. They are lacking in protein coding capacity and it seems that they...

5S ribosomal RNA database Y2K

Szymanski, Maciej, Barciszewska, Miroslawa Z., Barciszewski, Jan, Erdmann, Volker A.

This paper presents the updated version (Y2K) of the database of ribosomal 5S ribonucleic acids (5S rRNA) and their genes (5S rDNA), http://rose.man/poznan. pl/5SData/index.html . This edition of the...

Design of antisense oligonucleotides stabilized by locked nucleic acids

Kurreck, Jens, Wyszko, Eliza, Gillen, Clemens, Erdmann, Volker A.

The design of antisense oligonucleotides containing locked nucleic acids (LNA) was optimized and compared to intensively studied DNA oligonucleotides, phosphorothioates and 2′-O-methyl gapmers. In...

Comparison of different antisense strategies in mammalian cells using locked nucleic acids, 2′-O-methyl RNA, phosphorothioates and small interfering RNA

Grünweller, Arnold, Wyszko, Eliza, Bieber, Birgit, Jahnel, Ricarda, Erdmann, Volker A., Kurreck, Jens

Locked nucleic acids (LNAs) and double-stranded small interfering RNAs (siRNAs) are rather new promising antisense molecules for cell culture and in vivo applications. Here, we compare...

Noncoding regulatory RNAs database

Szymański, Maciej, Erdmann, Volker A., Barciszewski, Jan

The noncoding RNAs database is a collection of currently available sequence data on RNAs, which have no protein-coding capacity and have been implicated in regulation of cellular processes. The RNAs...

RNA cleaving ‘10-23’ DNAzymes with enhanced stability and activity

Schubert, Steffen, Gül, Deniz C., Grunert, Hans-Peter, Zeichhardt, Heinz, Erdmann, Volker A., Kurreck, Jens

‘10-23’ DNAzymes can be used to cleave any target RNA in a sequence-specific manner. For applications in vivo, they have to be stabilised against nucleolytic attack by the introduction of...

Terminal heterogeneity and corrections of the nucleotide sequence of 5S rRNA from an extreme thermophile, Thermus thermophilus HB8

Komiya, Hiroyuki, Kawakami, Makoto, Takemura, Shosuke, Kumagai, Izumi, Erdmann, Volker A.

The nucleotide sequence of 5S rRNA from an extreme thermophile Thermusthermophilus HB8 has been re-examined and determined as [Formula: see text] The present study could resolve previously ambiguous...

The nucleotide sequence of 5S rRNA from an extreme thermophile, Thermus thermophilus HB8

Kumagai, Izumi, Digweed, Martin, Erdmann, Volker A., Watanabe, Kimitsuna, Oshima, Tairo

Using 3′- and 5′-end labelling sequencing techniques, the following primary structure for Thermusthermophilus HB8 5S RNA could be determined: pAA (U)...

Compilation of 5S rRNA and 5S rRNA gene sequences

Specht, Thomas, Wolters, Jörn, Erdmann, Volker A.

This is an update for the 5S rRNA sequences of the BERLIN RNA DATABANK last published in 1990 (1). The new entry consists of 25 eubacterial and 2 eukaryotic 5S rRNA sequences and 10 plant 5S rRNA...

Compilation of 5S rRNA and 5S rRNA gene sequences

Specht, Thomas, Wolters, Jörn, Erdmann, Volker A.

The BERLIN RNA DATABANK as of Dezember 31, 1989, contains a total of 667 sequences of 5S rRNAs or their genes, which is an increase of 114 new sequence entries over the last compilation (1). It...

A New Transfer RNA Fragment Reaction: TpΨpCpGp Bound to a Ribosome-Messenger RNA Complex Induces the Synthesis of Guanosine Tetra- and Pentaphosphates

Richter, Dietmar, Erdmann, Volker A., Sprinzl, Mathias

The tetranucleotide TpΨpCpGp, when bound to Escherichia coli 50S ribosomal subunits, can replace intact tRNA in the stringent factor-directed synthesis of guanosine tetra- and pentaphosphates. The...

ATPase and GTPase Activities Associated with a Specific 5S RNA-Protein Complex*

Horne, Janet R., Erdmann, Volker A.

An RNA-protein complex consisting of 5S RNA and two ribosomal proteins, B-L5 and B-L22, was isolated from Bacillus stearothermophilus ribosomes and found to be active in GTP hydrolysis. This activity...

Identification of Chloramphenicol-Binding Protein in Escherichia coli Ribosomes by Affinity Labeling*

Pongs, Olaf, Bald, Rolf, Erdmann, Volker A.

Monoiodoamphenicol, a synthetic analogue of chloramphenicol, has been shown by competition experiments with chloramphenicol and lincomycin to bind at the same site of 70S ribosomes as...

Application of locked nucleic acids to improve aptamer in vivo stability and targeting function

Schmidt, Kathrin S., Borkowski, Sandra, Kurreck, Jens, Stephens, Andrew W., Bald, Rolf, Hecht, Maren, ...

Aptamers are powerful candidates for molecular imaging applications due to a number of attractive features, including rapid blood clearance and tumor penetration. We carried out structure–activity...

5 S rRNA: structure and interactions.

Szymański, Maciej, Barciszewska, Mirosława Z, Erdmann, Volker A, Barciszewski, Jan

5 S rRNA is an integral component of the large ribosomal subunit in all known organisms. Despite many years of intensive study, the function of 5 S rRNA in the ribosome remains unknown. Advances in...

The non-coding RNAs as riboregulators

Erdmann, Volker A., Barciszewska, Miroslawa Z., Szymanski, Maciej, Hochberg, Abraham, Groot, Nathan De, Barciszewski, Jan

The non-coding RNAs database (http://biobases.ibch.poznan.pl/ncRNA/) contains currently available data on RNAs, which do not have long open reading frames and act as riboregulators. Non-coding RNAs...

5S Ribosomal RNA Database

Szymanski, Maciej, Barciszewska, Miroslawa Z., Erdmann, Volker A., Barciszewski, Jan

Ribosomal 5S RNA (5S rRNA) is an integral component of the large ribosomal subunit in all known organisms with the exception only of mitochondrial ribosomes of fungi and animals. It is thought to...

Non-coding, mRNA-like RNAs database Y2K

Erdmann, Volker A., Szymanski, Maciej, Hochberg, Abraham, Groot, Nathan De, Barciszewski, Jan

In last few years much data has accumulated on various non-translatable RNA transcripts that are synthesised in different cells. They are lacking in protein coding capacity and it seems that they...

5S ribosomal RNA database Y2K

Szymanski, Maciej, Barciszewska, Miroslawa Z., Barciszewski, Jan, Erdmann, Volker A.

This paper presents the updated version (Y2K) of the database of ribosomal 5S ribonucleic acids (5S rRNA) and their genes (5S rDNA), http://rose.man/poznan. pl/5SData/index.html . This edition of the...

Design of antisense oligonucleotides stabilized by locked nucleic acids

Kurreck, Jens, Wyszko, Eliza, Gillen, Clemens, Erdmann, Volker A.

The design of antisense oligonucleotides containing locked nucleic acids (LNA) was optimized and compared to intensively studied DNA oligonucleotides, phosphorothioates and 2′-O-methyl gapmers. In...

Comparison of different antisense strategies in mammalian cells using locked nucleic acids, 2′-O-methyl RNA, phosphorothioates and small interfering RNA

Grünweller, Arnold, Wyszko, Eliza, Bieber, Birgit, Jahnel, Ricarda, Erdmann, Volker A., Kurreck, Jens

Locked nucleic acids (LNAs) and double-stranded small interfering RNAs (siRNAs) are rather new promising antisense molecules for cell culture and in vivo applications. Here, we compare...

Noncoding regulatory RNAs database

Szymański, Maciej, Erdmann, Volker A., Barciszewski, Jan

The noncoding RNAs database is a collection of currently available sequence data on RNAs, which have no protein-coding capacity and have been implicated in regulation of cellular processes. The RNAs...

RNA cleaving ‘10-23’ DNAzymes with enhanced stability and activity

Schubert, Steffen, Gül, Deniz C., Grunert, Hans-Peter, Zeichhardt, Heinz, Erdmann, Volker A., Kurreck, Jens

‘10-23’ DNAzymes can be used to cleave any target RNA in a sequence-specific manner. For applications in vivo, they have to be stabilised against nucleolytic attack by the introduction of...

Terminal heterogeneity and corrections of the nucleotide sequence of 5S rRNA from an extreme thermophile, Thermus thermophilus HB8

Komiya, Hiroyuki, Kawakami, Makoto, Takemura, Shosuke, Kumagai, Izumi, Erdmann, Volker A.

The nucleotide sequence of 5S rRNA from an extreme thermophile Thermusthermophilus HB8 has been re-examined and determined as [Formula: see text] The present study could resolve previously ambiguous...

The nucleotide sequence of 5S rRNA from an extreme thermophile, Thermus thermophilus HB8

Kumagai, Izumi, Digweed, Martin, Erdmann, Volker A., Watanabe, Kimitsuna, Oshima, Tairo

Using 3′- and 5′-end labelling sequencing techniques, the following primary structure for Thermusthermophilus HB8 5S RNA could be determined: pAA (U)...

Compilation of 5S rRNA and 5S rRNA gene sequences

Specht, Thomas, Wolters, Jörn, Erdmann, Volker A.

This is an update for the 5S rRNA sequences of the BERLIN RNA DATABANK last published in 1990 (1). The new entry consists of 25 eubacterial and 2 eukaryotic 5S rRNA sequences and 10 plant 5S rRNA...

Compilation of 5S rRNA and 5S rRNA gene sequences

Specht, Thomas, Wolters, Jörn, Erdmann, Volker A.

The BERLIN RNA DATABANK as of Dezember 31, 1989, contains a total of 667 sequences of 5S rRNAs or their genes, which is an increase of 114 new sequence entries over the last compilation (1). It...

A New Transfer RNA Fragment Reaction: TpΨpCpGp Bound to a Ribosome-Messenger RNA Complex Induces the Synthesis of Guanosine Tetra- and Pentaphosphates

Richter, Dietmar, Erdmann, Volker A., Sprinzl, Mathias

The tetranucleotide TpΨpCpGp, when bound to Escherichia coli 50S ribosomal subunits, can replace intact tRNA in the stringent factor-directed synthesis of guanosine tetra- and pentaphosphates. The...

ATPase and GTPase Activities Associated with a Specific 5S RNA-Protein Complex*

Horne, Janet R., Erdmann, Volker A.

An RNA-protein complex consisting of 5S RNA and two ribosomal proteins, B-L5 and B-L22, was isolated from Bacillus stearothermophilus ribosomes and found to be active in GTP hydrolysis. This activity...

Identification of Chloramphenicol-Binding Protein in Escherichia coli Ribosomes by Affinity Labeling*

Pongs, Olaf, Bald, Rolf, Erdmann, Volker A.

Monoiodoamphenicol, a synthetic analogue of chloramphenicol, has been shown by competition experiments with chloramphenicol and lincomycin to bind at the same site of 70S ribosomes as...

Application of locked nucleic acids to improve aptamer in vivo stability and targeting function

Schmidt, Kathrin S., Borkowski, Sandra, Kurreck, Jens, Stephens, Andrew W., Bald, Rolf, Hecht, Maren, ...

Aptamers are powerful candidates for molecular imaging applications due to a number of attractive features, including rapid blood clearance and tumor penetration. We carried out structure–activity...

5 S rRNA: structure and interactions.

Szymański, Maciej, Barciszewska, Mirosława Z, Erdmann, Volker A, Barciszewski, Jan

5 S rRNA is an integral component of the large ribosomal subunit in all known organisms. Despite many years of intensive study, the function of 5 S rRNA in the ribosome remains unknown. Advances in...

Noncoding RNAs database (ncRNAdb)

Szymanski, Maciej, Erdmann, Volker A., Barciszewski, Jan

The noncoding RNA database (ncRNAdb) was created as a source of information on RNA molecules, which do not possess protein-coding capacity. It is now widely accepted that, in addition to...

Detection of Oncofetal H19 RNA in Rheumatoid Arthritis Synovial Tissue

Stuhlmüller, Bruno, Kunisch, Elke, Franz, Juliane, Martinez-Gamboa, Lorena, Hernandez, Maria M., Pruss, Axel, ...

The expression of oncofetal H19 RNA and its localization/cellular source was analyzed in synovial tissue (ST) and isolated synovial macrophages (Mφ) or synovial fibroblasts (SFBs) by reverse...

Crystallization and preliminary X-ray diffraction analysis of a tRNASer acceptor-stem microhelix

Förster, Charlotte, Krauss, Norbert, Brauer, Arnd B. E., Szkaradkiewicz, Karol, Brode, Svenja, Hennig, Klaus, ...

In order to investigate tRNA identity elements, an elongator tRNASer acceptor-stem helix was crystallized and a data set was collected to 1.8 Å resolution aiming at a comparison with the...

Comparative crystallization and preliminary X-ray diffraction studies of locked nucleic acid and RNA stems of a tenascin C-binding aptamer

Förster, Charlotte, Brauer, Arnd B. E., Brode, Svenja, Schmidt, Kathrin S., Perbandt, Markus, Meyer, Arne, ...

Locked nucleic acid (LNA) nucleotides are RNA analogues with a useful additional conformational constraint; the current investigation will provide the first crystallographic view of an all-LNA duplex.