Viviane BOULO

Adaptation of the sea-bass (Dicentrarchus Labrax) to fresh water: Role of aquaporins and Na+/K+-ATPases (2008)

GIFFARD MENA, I, LORIN NEBEL, C, CHARMANTIER, G, CASTILLE, R, BOULO, Viviane

Sea-bass (Dicentrarchus labrax) grow under different salinity regimes, from the open sea to lagoons and even rivers, but some mortality has been recorded in juvenile stages when exposed to low...

Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes (2007)

SAMAIN, Jean-Francois, DEGREMONT, Lionel, SOLETCHNIK, Patrick, HAURE, Joel, BEDIER, Edouard, ROPERT, Michel, ...

Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and...

Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis (2007)

TIRAPE, Ana, BACQUE, Coralie, BRIZARD, Raphael, VANDENBULCKE, Franck, BOULO, Viviane

The work presented here reports the expression of immune-related genes during ontogenesis in the oyster Crassostrea gigar. Expression patterns of 18 selected genes showed that RNAs detected in...

Recent advances in EST sequencing in Crassostrea gigas: towards the sequencing of the Pacific oyster genome (2007)

BOUDRY, Pierre, HUVET, Arnaud, LAPEGUE, Sylvie, SAUVAGE, Christopher, TANGUY, A., MORAGA, D., ...

Rationale for EST and genome sequencing of the Pacific oyster: • its membership of the Lophotrochozoa, an little studied branch of the Eukaryotes, • its worldwide aquacultural importance (4.2...

First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway (2007)

MONTAGNANI, Caroline, AVARRE, Jean-Christophe, DE LORGERIL, Julien, QUIQUAND, M, BOULO, Viviane, ESCOUBAS, Jean-Michel

In a previous work, we characterized a Crassostrea gigas cPNA (Cg-timp) encoding a protein which presents all the features of vertebrate tissue inhibitor of metalloproteinase (TIMP). The expression...

Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32 (2006)

LABREUCHE, Yannick, LAMBERT, Christophe, SOUDANT, Philippe, BOULO, Viviane, HUVET, Arnaud, NICOLAS, Jean-Louis

The strategies used by bacterial pathogens to circumvent host defense mechanisms remain largely undefined in bivalve molluscs. In this study, we investigated experimentally the interactions between...

The Na+/K+/2Cl(-) cotransporter in the sea bass Dicentrarchus labrax during ontogeny: involvement in osmoregulation (2006)

LORIN, N, BOULO, Viviane, BODINIER, C, CHARMANTIER, G

This study combines a cellular and molecular analysis of the Na+/K+/2Cl(-) cotransporter (NKCC) to determine the osmoregulatory role of this protein in different tissues during the ontogeny of the...

Monoclonal antibody against a gill Rickettsiales-like organism of Pecten maxiumus (Bivalvia): application to indirect immunofluorescence diagnosis (1992)

LE GALL, Gyslaine, MOURTON, Chantal, BOULO, Viviane, PAOLUCCI, Francis, PAU, Bernard, MIALHE, Eric

Using hybridoma technology, monoclonal antibodies (MABs) were prepared against a Rickettsiales-like organism (RLO) from the gills of the Saint-Jacques scallop Pecten maximus. The 5D7 MAB was then...

A direct monoclonal antibody sandwich immunoassay for detection of Bonamia ostreae (Ascetospora) in hemolymph samples of the flat oyster Ostrea edulis (Mollusca : Bivalvia) (1992)

COCHENNEC, Nathalie, HERVIO, Dominique, PANATIER, Bernard, BOULO, Viviane, MIALHE, Eric, ROGIER, Hervé, ...

Currently recognition of molluscan diseases relies heavily upon light microscopic examination of stained histological sections of molluscan tissues Such histopathological methods are time consuming...

Monoclonal antibodies against Bonamia ostreae (Protozoa: Ascetospera), an intrahaemocytic parasite of flat oyster Ostrea edulis (Mollusca : Bivlavia) (1991)

ROGIER, Hervé, HERVIO, Dominique, BOULO, Viviane, CLAVIES, Christine, HERVAUD, Eliane, BACHERE, Evelyne, ...

The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with...

Preparation and characterization of monoclonal antibodies agains neoplastic hemocytes of Mytilus edulis (Bivalvia) (1991)

NOEL, Danielle, BOULO, Viviane, CHAGOT, Dominique, MIALHE, Eric, PAOLUCCI, Francis, CLAVIES, Christine, ...

Mytilus edulis develop progressive and fatal hemocytic neoplasia. We have generated monoclonal antibodies using neoplastic hemocytes from highly infected individuals collected from Puget Sound in...

In vitro culture of mollusc hemocytes. Functional study of burst respiratory activity and analysis of interactions with protozoan and procaryotic pathogens (1991)

BOULO, Viviane, HERVIO, Dominique, MORVAN, Annie, BACHERE, Evelyne, MIALHE, Eric

The chief importance of hemocytes as immune effectors in molluscs has led to develop researches for in vitro culture. The chemiluminescence technique has been used to estimate the phagocytosis...

Serological and analysis of Bonamia in Ostrea edulis and Tiostrea lutaria using polyclonal and monoclonal antibodies (1988)

MIALHE, Eric, BOULO, Viviane, ELSTON, Ralph, HILL, Barry, HINE, Michel, MONTES, Jaime, ...

Murine moncolonal and polyclonal antibodies to antigesn of Bonamia ostreae were assessed for serological comparaison of parasites associated with different geographical originns of flat oyster,...

Bonamiasis : a model study of diseases in marine molluscs (1988)

GRIZEL, Henri, MIALHE, Eric, CHAGOT, Dominique, BOULO, Viviane, BACHERE, Evelyne

Research on bonamiasis, an epizootic disease of the edible oyster Ostrea edu/is caused by the protozoan Bonamia ostreae, is discussed in relation to oyster fanning, research technology,...

Monoclonal antibodies : a tool for molluscan pathology (1988)

MIALHE, Eric, BOULO, Viviane, GRIZEL, Henri, ROGIER, Hervé, PAOLUCCI, Francis

Hybridoma technology is reviewed, and the characteristics of monoclonal antibodies are compared with those of polyclonal antibodies. The contribution of monoclonal antibodies to molluscan pathology...

Bivale mollusc cell culture (1988)

MIALHE, Eric, BOULO, Viviane, GRIZEL, Henri

The historical background of bivalve cell culture and its potential uses in molluscan pathology are reviewed. Then, primary cultures for the study of some host-pathogen interactions at the cellular...

An infection of Ruditapes decussatus (Bivalvia) by Rickettsia (1987)

MIALHE, Eric, CHAGOT, Dominique, BOULO, Viviane, COMPS, Michel, RUANO, Francisco, GRIZEL, Henri

The authors report the presence of a Rickettsia in Ruditapes decussatus from natural populations of the Algarve (Portugal). Light microscopic shows infections of varying importance in gill tissues....

Histological study of a cellular reaction in Ruditpaes decussatus infected by a protozoan (1987)

CHAGOT, Dominique, COMPS, Michel, BOULO, Viviane, RUANO, Francisco, GRIZEL, Henri

An infection caused by a Perkinsus -like parasite has been observed in Ruditapes decussatus from Portugal (Comps and Chagot, 1987). Following a pathological study carried out on clams affected by...