Victoria J. Broughton-Head

Kinetic studies on the formation of DNA triplexes containing the nucleoside analogue 2 '-O-(2-aminoethyl)-5-(3-amino-1-propynyl)uridine (2008)

Rusling, David A., Broughton-Head, Victoria J., Tuck, Alex, Khairallah, Hannah, Osborne, Sadie D., Brown, Tom, ...

We have examined the kinetics of triple helix formation of oligonucleotides that contain the nucleotide analogue 2-O-(2-aminoethyl)-5-(3-amino-1-propynyl)uridine (bis-amino-U, BAU), which forms very...

Photoinduced crosslinking of double-helical DNA by psoralen covalently linked to a triple helix-forming oligonucleotide under near-physiological conditions (2007)

Li, Hong, Broughton-Head, Victoria J., Fox, Keith R., Brown, Tom

Stable triplexes have been generated under near-physiological conditions by the introduction of the C and T base analogues 3-methyl-2-aminopyridine-2 '-deoxyriboside and...

Footprinting: A method for determining the sequence selectivity, affinity and kinetics of DNA-binding ligands. [In: Methods Related to DNA Sequence Recognition] (2007)

Hampshire, Andrew J., Rusling, David A., Broughton-Head, Victoria J., Fox, Keith R.

Footprinting is a simple method for assessing the sequence selectivity of DNA-binding ligands. The method is based on the ability of the ligand to protect DNA from cleavage at its binding site. This...

Triplex staples: DNA double-strand cross-linking at internal and terminal sites using psoralen-containing triplex-forming oligonucleotides (2006)

Li, Hong, Broughton-Head, Victoria J., Peng, Guomei, Powers, Vicki E.C., Ovens, Matthew J., Fox, Keith R.., ...

A method has been developed to attach 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen to the 5 position of thymine bases during solid-phase oligonucleotide synthesis. UV irradiation of triplex-forming...

Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH (2005)

Rusling, David A., Le Strat, Loic, Powers, Vicki E.C., Broughton-Head, Victoria J., Booth, James, Lack, Oliver, ...

We have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair oligopurine oligopyrimidine sequence, using oligonucleotides that contain combinations of...

Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH (2005)

Rusling, David A., Le Strat, Loic, Powers, Vicki E.C., Broughton-Head, Victoria J., Booth, James, Lack, Oliver, ...

We have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair oligopurine · oligopyrimidine sequence, using oligonucleotides that contain combinations of...