Brett, Wolfgang, Mandinova, Anna, Aebi, Ueli, Zerkowski, Hans-Reinhard
No abstract available.
Fahrenkrog, Birthe, Hübner, Wolfgang, Mandinova, Anna, Panté, Nelly, Keller, Walter, Aebi, Ueli
The bidirectional nucleocytoplasmic transport of macromolecules is mediated by the nuclear pore complex (NPC) which, in yeast, is composed of ∼30 different proteins (nucleoporins). Pre-embedding...
An autonomous folding unit mediates the assembly of two-stranded coiled coils
Kammerer, Richard A., Schulthess, Therese, Landwehr, Ruth, Lustig, Ariel, Engel, Jürgen, Aebi, Ueli, ...
Subunit oligomerization of many proteins is mediated by coiled-coil domains. Although the basic features contributing to the thermodynamic stability of coiled coils are well understood, the...
Brault, Véronique, Sauder, Ursula, Reedy, Mary C., Aebi, Ueli, Schoenenberger, Cora-Ann
We have tested the impact of tags on the structure and function of indirect flight muscle (IFM)-specific Act88F actin by transforming mutant Drosophila melanogaster, which do not express endogenous...
Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins
Lutzmann, Malik, Kunze, Ruth, Buerer, Andrea, Aebi, Ueli, Hurt, Ed
Now that it is likely that all yeast nucleoporins are known, one of the ultimate goals is the in vitro assembly of the entire nuclear pore complex from its ∼30 individual components. Here, we...
Strelkov, Sergei V., Herrmann, Harald, Geisler, Norbert, Wedig, Tatjana, Zimbelmann, Ralf, Aebi, Ueli, ...
Intermediate filaments (IFs) are key components of the cytoskeleton in higher eukaryotic cells. The elementary IF ‘building block’ is an elongated coiled-coil dimer consisting of four consecutive...
Sac3 Is an mRNA Export Factor That Localizes to Cytoplasmic Fibrils of Nuclear Pore Complex
Lei, Elissa P., Stern, Charlene A., Fahrenkrog, Birthe, Krebber, Heike, Moy, Terence I., Aebi, Ueli, ...
In eukaryotes, mRNAs are transcribed in the nucleus and exported to the cytoplasm for translation to occur. Messenger RNAs complexed with proteins referred to as ribonucleoparticles are recognized...
Lamin A/C–dependent Localization of Nesprin-2, a Giant Scaffolder at the Nuclear Envelope
Libotte, Thorsten, Zaim, Hafida, Abraham, Sabu, Padmakumar, V. C., Schneider, Maria, Lu, Wenshu, ...
The vertebrate proteins Nesprin-1 and Nesprin-2 (also referred to as Enaptin and NUANCE) together with ANC-1 of Caenorhabditis elegans and MSP-300 of Drosophila melanogaster belong to a novel family...
Nuclear Actin Extends, with No Contraction in Sight
Within the past two years, actin has been implicated in eukaryotic gene transcription by all three classes of RNA polymerase. Moreover, within just the past year, actin has been identified as a...
Stolz, Martin, Raiteri, Roberto, Daniels, A. U., VanLandingham, Mark R., Baschong, Werner, Aebi, Ueli
Cartilage stiffness was measured ex vivo at the micrometer and nanometer scales to explore structure-mechanical property relationships at smaller scales than has been done previously. A method was...
Bär, Harald, Mücke, Norbert, Kostareva, Anna, Sjöberg, Gunnar, Aebi, Ueli, Herrmann, Harald
Desmin is the major intermediate filament (IF) protein of muscle. Recently, mutations of the desmin gene have been reported to cause familial or sporadic forms of human skeletal, as well as cardiac,...
Flexible phenylalanine-glycine nucleoporins as entropic barriers to nucleocytoplasmic transport
Lim, Roderick Y. H., Huang, Ning-Ping, Köser, Joachim, Deng, Jie, Lau, K. H. Aaron, Schwarz-Herion, Kyrill, ...
Natively unfolded phenylalanine-glycine (FG)-repeat domains are alleged to form the physical constituents of the selective barrier-gate in nuclear pore complexes during nucleocytoplasmic transport....
Fahrenkrog, Birthe, Hübner, Wolfgang, Mandinova, Anna, Panté, Nelly, Keller, Walter, Aebi, Ueli
The bidirectional nucleocytoplasmic transport of macromolecules is mediated by the nuclear pore complex (NPC) which, in yeast, is composed of ∼30 different proteins (nucleoporins). Pre-embedding...
An autonomous folding unit mediates the assembly of two-stranded coiled coils
Kammerer, Richard A., Schulthess, Therese, Landwehr, Ruth, Lustig, Ariel, Engel, Jürgen, Aebi, Ueli, ...
Subunit oligomerization of many proteins is mediated by coiled-coil domains. Although the basic features contributing to the thermodynamic stability of coiled coils are well understood, the...
Brault, Véronique, Sauder, Ursula, Reedy, Mary C., Aebi, Ueli, Schoenenberger, Cora-Ann
We have tested the impact of tags on the structure and function of indirect flight muscle (IFM)-specific Act88F actin by transforming mutant Drosophila melanogaster, which do not express endogenous...
Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins
Lutzmann, Malik, Kunze, Ruth, Buerer, Andrea, Aebi, Ueli, Hurt, Ed
Now that it is likely that all yeast nucleoporins are known, one of the ultimate goals is the in vitro assembly of the entire nuclear pore complex from its ∼30 individual components. Here, we...
Strelkov, Sergei V., Herrmann, Harald, Geisler, Norbert, Wedig, Tatjana, Zimbelmann, Ralf, Aebi, Ueli, ...
Intermediate filaments (IFs) are key components of the cytoskeleton in higher eukaryotic cells. The elementary IF ‘building block’ is an elongated coiled-coil dimer consisting of four consecutive...
Sac3 Is an mRNA Export Factor That Localizes to Cytoplasmic Fibrils of Nuclear Pore Complex
Lei, Elissa P., Stern, Charlene A., Fahrenkrog, Birthe, Krebber, Heike, Moy, Terence I., Aebi, Ueli, ...
In eukaryotes, mRNAs are transcribed in the nucleus and exported to the cytoplasm for translation to occur. Messenger RNAs complexed with proteins referred to as ribonucleoparticles are recognized...
Lamin A/C–dependent Localization of Nesprin-2, a Giant Scaffolder at the Nuclear Envelope
Libotte, Thorsten, Zaim, Hafida, Abraham, Sabu, Padmakumar, V. C., Schneider, Maria, Lu, Wenshu, ...
The vertebrate proteins Nesprin-1 and Nesprin-2 (also referred to as Enaptin and NUANCE) together with ANC-1 of Caenorhabditis elegans and MSP-300 of Drosophila melanogaster belong to a novel family...
Bär, Harald, Mücke, Norbert, Kostareva, Anna, Sjöberg, Gunnar, Aebi, Ueli, Herrmann, Harald
Desmin is the major intermediate filament (IF) protein of muscle. Recently, mutations of the desmin gene have been reported to cause familial or sporadic forms of human skeletal, as well as cardiac,...
Nuclear Actin Extends, with No Contraction in Sight
Within the past two years, actin has been implicated in eukaryotic gene transcription by all three classes of RNA polymerase. Moreover, within just the past year, actin has been identified as a...
Stolz, Martin, Raiteri, Roberto, Daniels, A. U., VanLandingham, Mark R., Baschong, Werner, Aebi, Ueli
Cartilage stiffness was measured ex vivo at the micrometer and nanometer scales to explore structure-mechanical property relationships at smaller scales than has been done previously. A method was...
Sokolova, Anna V., Kreplak, Laurent, Wedig, Tatjana, Mücke, Norbert, Svergun, Dmitri I., Herrmann, Harald, ...
Intermediate filaments (IFs), along with microtubules, microfilaments, and associated cross-bridging proteins, constitute the cytoskeleton of metazoan cells. While crystallographic data on the dimer...
Boerries, Melanie, Most, Patrick, Gledhill, Jonathan R., Walker, John E., Katus, Hugo A., Koch, Walter J., ...
S100A1, a Ca2+-sensing protein of the EF-hand family that is expressed predominantly in cardiac muscle, plays a pivotal role in cardiac contractility in vitro and in vivo. It has recently been...
Single Hepatitis-B Virus Core Capsid Binding to Individual Nuclear Pore Complexes in HeLa Cells
Lill, Yoriko, Lill, Markus A., Fahrenkrog, Birthe, Schwarz-Herion, Kyrill, Paulillo, Sara, Aebi, Ueli, ...
We investigate the interaction of hepatitis B virus capsids lacking a nuclear localization signal with nuclear pore complexes (NPCs) in permeabilized HeLa cells. Confocal and wide-field optical...
Towards reconciling structure and function in the nuclear pore complex
Lim, Roderick Y.H., Aebi, Ueli, Fahrenkrog, Birthe
The spatial separation between the cytoplasm and the cell nucleus necessitates the continuous exchange of macromolecular cargo across the double-membraned nuclear envelope. Being the only passageway...
A Correlative Analysis of Actin Filament Assembly, Structure, and Dynamics
Steinmetz, Michel O., Goldie, Kenneth N., Aebi, Ueli
The effect of the type of metal ion (i.e., Ca2+, Mg2+, or none) bound to the high-affinity divalent cation binding site (HAS) of actin on filament assembly, structure, and dynamics was investigated...
Molecular Architecture of the Yeast Nuclear Pore Complex: Localization of Nsp1p Subcomplexes
Fahrenkrog, Birthe, Hurt, Eduard C., Aebi, Ueli, Panté, Nelly
The nuclear pore complex (NPC), a supramolecular assembly of ∼100 different proteins (nucleoporins), mediates bidirectional transport of molecules between the cytoplasm and the cell nucleus....
Structure and Assembly of the Nup84p Complex
Siniossoglou, Symeon, Lutzmann, Malik, Santos-Rosa, Helena, Leonard, Kevin, Mueller, Shirley, Aebi, Ueli, ...
The Nup84p complex consists of five nucleoporins (Nup84p, Nup85p, Nup120p, Nup145p-C, and Seh1p) and Sec13p, a bona fide subunit of the COPII coat complex. We show that a pool of green fluorescent...
Laminopathic mutations interfere with the assembly, localization, and dynamics of nuclear lamins
Wiesel, Naama, Mattout, Anna, Melcer, Shai, Melamed-Book, Naomi, Herrmann, Harald, Medalia, Ohad, ...
Lamins are nuclear intermediate filament proteins and the major building blocks of the nuclear lamina. Besides providing nuclear shape and mechanical stability, lamins are required for chromatin...
The highly conserved nuclear lamin Ig-fold binds to PCNA: its role in DNA replication
Shumaker, Dale K., Solimando, Liliana, Sengupta, Kaushik, Shimi, Takeshi, Adam, Stephen A., Grunwald, Antje, ...
This study provides insights into the role of nuclear lamins in DNA replication. Our data demonstrate that the Ig-fold motif located in the lamin C terminus binds directly to proliferating cell...