Udo Häcker

piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila

Häcker, Udo, Nystedt, Sverker, Barmchi, Mojgan Padash, Horn, Carsten, Wimmer, Ernst A.

P element-mediated mutagenesis has been used to disrupt an estimated 25% of genes essential for Drosophila adult viability. Mutation of all genes in the fly genome, however, poses a problem, because...

slalom encodes an adenosine 3′-phosphate 5′-phosphosulfate transporter essential for development in Drosophila

Lüders, Florian, Segawa, Hiroaki, Stein, David, Selva, Erica M., Perrimon, Norbert, Turco, Salvatore J., ...

Sulfation of all macromolecules entering the secretory pathway in higher organisms occurs in the Golgi and requires the high-energy sulfate donor adenosine 3′-phosphate 5′-phosphosulfate. Here we...

DRhoGEF2 encodes a member of the Dbl family of oncogenes and controls cell shape changes during gastrulation in Drosophila

Häcker, Udo, Perrimon, Norbert

We have identified a gene, DRhoGEF2, which encodes a putative guanine nucleotide exchange factor belonging to the Dbl family of oncogenes. DRhoGEF2 function is essential for the coordination of cell...

piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics.

Horn, Carsten, Offen, Nils, Nystedt, Sverker, Häcker, Udo, Wimmer, Ernst A

Transposon mutagenesis provides a fundamental tool for functional genomics. Here we present a non-species-specific, combined enhancer detection and binary expression system based on the transposable...

piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila

Häcker, Udo, Nystedt, Sverker, Barmchi, Mojgan Padash, Horn, Carsten, Wimmer, Ernst A.

P element-mediated mutagenesis has been used to disrupt an estimated 25% of genes essential for Drosophila adult viability. Mutation of all genes in the fly genome, however, poses a problem, because...

slalom encodes an adenosine 3′-phosphate 5′-phosphosulfate transporter essential for development in Drosophila

Lüders, Florian, Segawa, Hiroaki, Stein, David, Selva, Erica M., Perrimon, Norbert, Turco, Salvatore J., ...

Sulfation of all macromolecules entering the secretory pathway in higher organisms occurs in the Golgi and requires the high-energy sulfate donor adenosine 3′-phosphate 5′-phosphosulfate. Here we...

DRhoGEF2 encodes a member of the Dbl family of oncogenes and controls cell shape changes during gastrulation in Drosophila

Häcker, Udo, Perrimon, Norbert

We have identified a gene, DRhoGEF2, which encodes a putative guanine nucleotide exchange factor belonging to the Dbl family of oncogenes. DRhoGEF2 function is essential for the coordination of cell...

piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics.

Horn, Carsten, Offen, Nils, Nystedt, Sverker, Häcker, Udo, Wimmer, Ernst A

Transposon mutagenesis provides a fundamental tool for functional genomics. Here we present a non-species-specific, combined enhancer detection and binary expression system based on the transposable...

DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo

Padash Barmchi, Mojgan, Rogers, Stephen, Häcker, Udo

Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls...

DRhoGEF2 and Diaphanous Regulate Contractile Force during Segmental Groove Morphogenesis in the Drosophila Embryo

Mulinari, Shai, Barmchi, Mojgan Padash, Häcker, Udo

Morphogenesis of the Drosophila embryo is associated with dynamic rearrangement of the actin cytoskeleton mediated by small GTPases of the Rho family. These GTPases act as molecular switches that are...