Scientific Commons: Tristan Piolot

The last 59 amino acids of Smoothened cytoplasmic tail directly bind the protein kinase Fused and negatively regulate the Hedgehog pathway. (2007)

Malpel, Sébastien, Claret, Sandra, Sanial, Matthieu, Brigui, Amira, Piolot, Tristan, Daviet, Laurent, ...

The Hedgehog (HH) signaling pathway is crucial for the development of many organisms and its inappropriate activation is involved in numerous cancers. HH signal controls the traffic and activity of...

The last 59 amino acids of Smoothened cytoplasmic tail directly bind the protein kinase Fused and negatively regulate the Hedgehog pathway. (2007)

Malpel, Sébastien, Claret, Sandra, Sanial, Matthieu, Brigui, Amira, Piolot, Tristan, Daviet, Laurent, ...

The Hedgehog (HH) signaling pathway is crucial for the development of many organisms and its inappropriate activation is involved in numerous cancers. HH signal controls the traffic and activity of...

Homo- and Hetero-oligomerization of {beta}-Arrestins in Living Cells (2005)

Storez, Hélène, G. H. Scott, Mark, Issafras, Hassan, Burtey, Anne, Benmerah, Alexandre, Muntaner, Olivier, ...

Arrestins are important proteins, which regulate the function of serpentine heptahelical receptors and contribute to multiple signaling pathways downstream of receptors. The ubiquitous arrestins are...

A new insertion in the HIV-1 reverse transcriptase gene inducing major resistance to non-nucleoside reverse transcriptase inhibitors (2005)

Amiel, Corinne, Desire, Nathalie, Schneider, Véronique, Delphin, Nathalie, Race, Ester, Clavel, Françoise, ...

We identified an HIV-1 isolate with a 3 base pairs insertion in the 100105 region of the reverse transcriptase gene (RT) along with a G190E and a V75A mutation. Virus carrying the insertion alone...

Photoconversion of YFP into a CFP-like species during acceptor photobleaching FRET experiments (2005)

Valentin, Guillaume, Verheggen, Céline, Piolot, Tristan, Neel, Henry, Coppey-Moisan, Maïté, Bertrand, Edouard

Mapping EBNA-1 Domains Involved in Binding to Metaphase Chromosomes

Marechal, Vincent, Dehee, Axelle, Chikhi-Brachet, Roxane, Piolot, Tristan, Coppey-Moisan, Maité, Nicolas, Jean-Claude

The Epstein-Barr virus (EBV) genome can persist in dividing human B cells as multicopy circular episomes. Viral episomes replicate in synchrony with host cell DNA and are maintained at a relatively...

Close but Distinct Regions of Human Herpesvirus 8 Latency-Associated Nuclear Antigen 1 Are Responsible for Nuclear Targeting and Binding to Human Mitotic Chromosomes

Piolot, Tristan, Tramier, Marc, Coppey, Maité, Nicolas, Jean-Claude, Marechal, Vincent

Human herpesvirus 8 is associated with all forms of Kaposi's sarcoma, AIDS-associated body cavity-based lymphomas, and some forms of multicentric Castleman's disease. Herpesvirus 8, like other...

Picosecond-hetero-FRET microscopy to probe protein-protein interactions in live cells.

Tramier, Marc, Gautier, Isabelle, Piolot, Tristan, Ravalet, Sylvie, Kemnitz, Klaus, Coppey, Jacques, ...

By using a novel time- and space-correlated single-photon counting detector, we show that fluorescence resonance energy transfer (FRET) between cyan fluorescent protein (CFP) and yellow fluorescent...