Tomoko Chiba

Toward a protein–protein interaction map of the budding yeast: A comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins

Ito, Takashi, Tashiro, Kosuke, Muta, Shigeru, Ozawa, Ritsuko, Chiba, Tomoko, Nishizawa, Mayumi, ...

Protein–protein interactions play pivotal roles in various aspects of the structural and functional organization of the cell, and their complete description is indispensable to thorough...

A comprehensive two-hybrid analysis to explore the yeast protein interactome

Ito, Takashi, Chiba, Tomoko, Ozawa, Ritsuko, Yoshida, Mikio, Hattori, Masahira, Sakaki, Yoshiyuki

Protein–protein interactions play crucial roles in the execution of various biological functions. Accordingly, their comprehensive description would contribute considerably to the functional...

Mutagenically Separated PCR Assay for Rapid Detection of M41L and K70R Zidovudine Resistance Mutations in CRF01_AE (Subtype E) Human Immunodeficiency Virus Type 1

Myint, Lay, Ariyoshi, Koya, Yan, Hua, Frater, Alexander J., Auwanit, Wattana, Pathipvanith, Panita, ...

A rapid zidovudine (ZDV) resistance genotypic assay was developed based on the mutagenically separated PCR (MS-PCR) technique to detect two ZDV-resistant mutations, M41L and K70R in CRF01_AE (subtype...

Novel Enzyme-Linked Minisequence Assay for Genotypic Analysis of Human Immunodeficiency Virus Type 1 Drug Resistance

Sugiura, Wataru, Shimada, Kazunori, Matsuda, Masakazu, Chiba, Tomoko, Myint, Lay, Okano, Aiko, ...

We constructed a novel tool for genotypic analysis of human immunodeficiency virus type 1 (HIV-1) drug resistance by using an enzyme-linked minisequence assay (ELMA). ELMA is a combination of...

Gag Non-Cleavage Site Mutations Contribute to Full Recovery of Viral Fitness in Protease Inhibitor-Resistant Human Immunodeficiency Virus Type 1

Myint, Lay, Matsuda, Masakazu, Matsuda, Zene, Yokomaku, Yoshiyuki, Chiba, Tomoko, Okano, Aiko, ...

It is well documented that human immunodeficiency virus type 1 (HIV-1) Gag cleavage site mutations (CSMs) emerge in conjunction with various HIV-1 mutations for protease inhibitor (PI) resistance and...

Barrier Proteins Remodel and Modify Chromatin To Restrict Silenced Domains†

Oki, Masaya, Valenzuela, Lourdes, Chiba, Tomoko, Ito, Takashi, Kamakaka, Rohinton T.

Transcriptionally active and inactive domains are frequently found adjacent to one another in the eukaryotic nucleus. To better understand the underlying mechanisms by which domains maintain opposing...

Transcriptional activators in yeast

Titz, Björn, Thomas, Sindhu, Rajagopala, Seesandra V., Chiba, Tomoko, Ito, Takashi, Uetz, Peter

Eukaryotic transcription activation domains (ADs) are not well defined on the proteome scale. We systematicallly tested ∼6000 yeast proteins for transcriptional activity using a yeast one-hybrid...

Toward a protein–protein interaction map of the budding yeast: A comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins

Ito, Takashi, Tashiro, Kosuke, Muta, Shigeru, Ozawa, Ritsuko, Chiba, Tomoko, Nishizawa, Mayumi, ...

Protein–protein interactions play pivotal roles in various aspects of the structural and functional organization of the cell, and their complete description is indispensable to thorough...

A comprehensive two-hybrid analysis to explore the yeast protein interactome

Ito, Takashi, Chiba, Tomoko, Ozawa, Ritsuko, Yoshida, Mikio, Hattori, Masahira, Sakaki, Yoshiyuki

Protein–protein interactions play crucial roles in the execution of various biological functions. Accordingly, their comprehensive description would contribute considerably to the functional...

Mutagenically Separated PCR Assay for Rapid Detection of M41L and K70R Zidovudine Resistance Mutations in CRF01_AE (Subtype E) Human Immunodeficiency Virus Type 1

Myint, Lay, Ariyoshi, Koya, Yan, Hua, Frater, Alexander J., Auwanit, Wattana, Pathipvanith, Panita, ...

A rapid zidovudine (ZDV) resistance genotypic assay was developed based on the mutagenically separated PCR (MS-PCR) technique to detect two ZDV-resistant mutations, M41L and K70R in CRF01_AE (subtype...

Novel Enzyme-Linked Minisequence Assay for Genotypic Analysis of Human Immunodeficiency Virus Type 1 Drug Resistance

Sugiura, Wataru, Shimada, Kazunori, Matsuda, Masakazu, Chiba, Tomoko, Myint, Lay, Okano, Aiko, ...

We constructed a novel tool for genotypic analysis of human immunodeficiency virus type 1 (HIV-1) drug resistance by using an enzyme-linked minisequence assay (ELMA). ELMA is a combination of...

Gag Non-Cleavage Site Mutations Contribute to Full Recovery of Viral Fitness in Protease Inhibitor-Resistant Human Immunodeficiency Virus Type 1

Myint, Lay, Matsuda, Masakazu, Matsuda, Zene, Yokomaku, Yoshiyuki, Chiba, Tomoko, Okano, Aiko, ...

It is well documented that human immunodeficiency virus type 1 (HIV-1) Gag cleavage site mutations (CSMs) emerge in conjunction with various HIV-1 mutations for protease inhibitor (PI) resistance and...

Barrier Proteins Remodel and Modify Chromatin To Restrict Silenced Domains†

Oki, Masaya, Valenzuela, Lourdes, Chiba, Tomoko, Ito, Takashi, Kamakaka, Rohinton T.

Transcriptionally active and inactive domains are frequently found adjacent to one another in the eukaryotic nucleus. To better understand the underlying mechanisms by which domains maintain opposing...

Transcriptional activators in yeast

Titz, Björn, Thomas, Sindhu, Rajagopala, Seesandra V., Chiba, Tomoko, Ito, Takashi, Uetz, Peter

Eukaryotic transcription activation domains (ADs) are not well defined on the proteome scale. We systematicallly tested ∼6000 yeast proteins for transcriptional activity using a yeast one-hybrid...