Rapid and efficient DNA strand cross-linking by click chemistry (2008)
Kocalka, Petr, El-Sagheer, Afaf H., Brown, Tom
Click chemistry has been used to covalently cross-link complementary DNA strands between bases to form very stable duplexes. Several alkyne- and azide-modified uracil monomers were used to evaluate...
Sandin, Peter, Borjesson, Karl, Li, Hong, Martensson, Jerker, Brown, Tom, Wilhelmsson, L. Marcus, ...
This article presents the first evidence that the DNA base analogue 1,3-diaza-2-oxophenoxazine, tCO, is highly fluorescent, both as free nucleoside and incorporated in an arbitrary DNA structure. tCO...
A very stable cyclic DNA miniduplex with just two base pairs (2008)
El-Sagheer, Afaf. H., Kumar, Ravindra, Findlow, Stuart, Werner, Joern . M., Lane, Andrew. N., Brown, Tom
Rusling, David A., Broughton-Head, Victoria J., Tuck, Alex, Khairallah, Hannah, Osborne, Sadie D., Brown, Tom, ...
We have examined the kinetics of triple helix formation of oligonucleotides that contain the nucleotide analogue 2-O-(2-aminoethyl)-5-(3-amino-1-propynyl)uridine (bis-amino-U, BAU), which forms very...
One-to-one technology-enhanced learning: an opportunity for global research collaboration (2007)
Chan, Tak-Wai, Roschelle, Jeremy, Hsi, Sherry, Kinshuk, Kinshuk, Sharples, Mike, Brown, Tom, ...
Over the next 10 years, we anticipate that personal, portable, wirelessly-networked technologies will become ubiquitous in the lives of learners - indeed, in many countries, this is already a...
One-to-one technology-enhanced learning: an opportunity for global research collaboration (2007)
Chan, Tak-Wai, Roschelle, Jeremy, Hsi, Sherry, Kinshuk, Kinshuk, Sharples, Mike, Brown, Tom, ...
Over the next 10 years, we anticipate that personal, portable, wirelessly-networked technologies will become ubiquitous in the lives of learners - indeed, in many countries, this is already a...
Brennan, Lavinia, Peng, Guomei, Srinivasan, Natarajan, Fox, Keith R., Brown, Tom
The nucleoside analogues 2'-O-dimethylaminoethoxy uridine and 5-dimethylaminopropargyl deoxyuridine have been synthesised and incorporated into oligonucleotides. Their triplex-stabilising properties...
Gerrard, Simon R., Srinivasan, Natarajan, Fox, Keith R., Brown, Tom
Triplex-mediated recognition of Py.Pu base pairs in DNA is a greater challenge than for Pu.Py base pairs as fewer hydrogen bonds are presented for binding in the major groove. Initial studies on...
Li, Hong, Broughton-Head, Victoria J., Fox, Keith R., Brown, Tom
Stable triplexes have been generated under near-physiological conditions by the introduction of the C and T base analogues 3-methyl-2-aminopyridine-2 '-deoxyriboside and...
Synthesis of anthraquinone oligonucleotides for triplex stabilization (2007)
Zhao, Zhengyun, Peng, Guomei, Michels, Jan, Fox, Keith R., Brown, Tom
The synthesis of two anthraquinone phosphoramidites is described. In both cases the anthraquinone moiety is attached via a linker to the 5-position of a uracil base, allowing incorporation at any...
Synthesis and properties of oligonucleotides containing a cholesterol thymidine monomer (2007)
Highly selective base-pair recognition makes DNA a suitable building block for orderly self-assembled structures. For some applications in nanotechnology DNA complexes need to be fixed onto surfaces....
Xiao, Qiang, Ranasinghe, Rohan T., Tang, Adrian M.P., Brown, Tom
Fluorescent thymidine analogues functionalised in the 5-position with the moieties naphthalenylethynyl (NeT), anthracenylethynyl (AeT) and anthracenylbuta-1,3-diynyl (AeeT) have been incorporated...
Sequence effects of single base loops in intramolecular quadruplex DNA (2007)
Rachwal, Phillip A., Brown, Tom, Fox, Keith R.
We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1′,2′-dideoxyribose in all three...
Effect of G-tract length on the topology and stability of intramolecular DNA quadruplexes (2007)
Rachwal, Philip A., Brown, Tom, Fox, Keith R.
G-Rich sequences are known to form four-stranded structures that are based on stacks of G-quartets, and sequences with the potential to adopt these structures are common in eukaryotic genomes....
Olaf Zawacki-Richter, Tom Brown, Rhena Delport
This paper reports on a survey investigating the role that differences in expectations and perceptions of mobile learning and mobile devices play in establishing mobile learning at an educational...
Kumar, Ravindra, El-Sagheer, Afaf, Tumpane, John, Lincoln, Per, Wilhelmsson, L. Marcus, Brown, Tom
The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide,...
Li, Hong, Broughton-Head, Victoria J., Peng, Guomei, Powers, Vicki E.C., Ovens, Matthew J., Fox, Keith R.., ...
A method has been developed to attach 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen to the 5 position of thymine bases during solid-phase oligonucleotide synthesis. UV irradiation of triplex-forming...
DNA triple-helix formation at target sites containing duplex mismatches (2006)
Rusling, David A., Brown, Tom, Fox, Keith R.
We have studied the formation of DNA triple helices at target sites that contain mismatches in the duplex target. Fluorescence melting studies were used to examine a series of parallel triple helices...
Hampshire, Andrew J., Khairallah, Hannah, Khalaf, Abedawn I., Ebrahimabadi, Abdolrasoul H., Waigh, Roger D., Suckling, Colin J., ...
We have used DNA footprinting and fluorescence melting experiments to study the sequence specific binding of an imidazole-containing isopropyl-substituted thiazole polyamide (thiazotropsin B) to DNA....
Rusling, David A., Le Strat, Loic, Powers, Vicki E.C., Broughton-Head, Victoria J., Booth, James, Lack, Oliver, ...
We have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair oligopurine · oligopyrimidine sequence, using oligonucleotides that contain combinations of...
An extra dimension in nucleic acid sequence recognition (2005)
Introduction 312 Triple helices in DNA 312 Chemically modified TFOs 313 Further development 316 Recognition of GC base pairs 316 Recognition of TA base pairs 316 Recognition of AT base pairs 317...
Quenching of CdSe quantum dot emission, a new approach for biosensing (2005)
Melvin, Tracy, Dyadyusha, Larysa, Yin, Huabing, Jaiswal, Supriya, Baumberg, Jeremy, Booy, Frank, ...
The emission of CdSe quantum dots linked to the 5-end of a DNA sequence is efficiently quenched by hybridisation with a complementary DNA strand with a gold nanoparticle attached at the 3-end;...
Quenching of CdSe quantum dot emission, a new approach for biosensing (2005)
Melvin, Tracy, Dyadyusha, Larysa, Yin, Huabing, Jaiswal, Supriya, Baumberg, Jeremy, Booy, Frank, ...
The emission of CdSe quantum dots linked to the 5-end of a DNA sequence is efficiently quenched by hybridisation with a complementary DNA strand with a gold nanoparticle attached at the 3-end;...
Wang, Yang, Rusling, David A., Powers, Vicki E.C., Lack, Oliver, Osborne, Sadie D., Fox, Keith R., ...
We have prepared the 2'-aminoethoxy derivative of the S nucleoside (2AES) and incorporated it into triplex-forming oligonucleotides for recognition of TA interruptions within a target oligopurine...
Four base recognition by triplex-forming oligonucleotides at physiological pH (2005)
Rusling, David.A., Powers, Vicki .E.C., Ranasinghe, Rohan .T., Wang, Yang, Osborne, Sadie .D., Brown, Tom., ...
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for...
DNA sequence recognition by an isopropyl substituted thiazole polyamide (2004)
James, Peter L., Merkina, Elena E., Khalaf, Abedawn I., Suckling, Colin J., Waigh, Roger D., Brown, Tom, ...
We have used DNA footprinting and fluorescence melting experiments to study the sequence-specific binding of a novel minor groove binding ligand (thiazotropsin A), containing an isopropyl substituted...
Darby, Richard A.J., Sollogoub, Matthieu, McKeen, Catherine, Brown, Lynda, Risitano, Antonina, Brown, Nicholas, ...
We have used oligonucleotides containing molecular beacons to determine melting profiles for intramolecular DNA duplexes, triplexes and quadruplexes (tetraplexes). The synthetic oligonucleotides used...
A proposed model of bisexual identity development. / (2000)
Thesis (M.S.)--Pacific University, 2000.
Thesis (Ph. D.)--City University, 2000.
Thesis (Ph. D.).
Berners-Price, Sue, Parkinson, John A., Chen, Yu., Murdoch, Piedad. Del Socorro, Guo, Zijian., Brown, Tom., ...
Retaining minority students at UW-Green Bay / (1993)
Includes bibliographical references (leaves 61-63).
Sandin, Peter, Börjesson, Karl, Li, Hong, Mårtensson, Jerker, Brown, Tom, Wilhelmsson, L. Marcus, ...
This article presents the first evidence that the DNA base analogue 1,3-diaza-2-oxophenoxazine, tCO, is highly fluorescent, both as free nucleoside and incorporated in an arbitrary DNA structure. tCO...
The construction of the credible: Epistolary transformations and the origins of the business letter
The letter is the formal mechanism for communication in the business community. Even contemporary advances in electronic mail have not yet displaced the legitimacy invested in this form of...
UNDERSTANDING MARKETS IN AFGHANISTAN: A CASE STUDY OF THE RAISIN MARKET
Lister, Sarah, Brown, Tom, Karaev, Zainiddin
Industrial Organization,