Boyington, Jeffrey C., Gladyshev, Vadim, Khangulov, Sergei V., Stadtman, Thressa C., Sun, Peter D.
Formate dehydrogenase H from Escherichia coli contains selenocysteine (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD) cofactors, and an Fe4S4 cluster at the active site and...
Selenoproteins—Tracing the Role of a Trace Element in Protein Function (2005)
Selenium is an important component of several enzymes, replacing sulfur in cysteine residues. Its discovery and significance are described in this primer.
Gladyshev, Vadim, Stadtman, Thressa C., Hatfield, Dolph L., Jeang, Kuan-Teh
It has been observed previously that plasma selenium and glutathione levels are subnormal in HIV-infected individuals, and plasma glutathione peroxidase activity is decreased. Under these conditions...
Gladyshev, Vadim, Jeang, Kuan-Teh, Stadtman, Thressa C.
The possible relationship of selenium to immunological function which has been suggested for decades was investigated in studies on selenuim metabolism in human T cells. One of the major 75Se-labeled...
Characterization of Crystalline Formate Dehydrogenase H from Escherichia coli (1996)
Gladyshev, Vadim N., Boyington, Jeffrey C., Khangulov, Sergei V., Grahame, David, Stadtman, Thressa C., Sun, Peter D.
The selenocysteine-containing formate dehydrogenase H (FDH) is an 80-kDa component of the Escherichia coli formate-hydrogen lyase complex. The molybdenumcoordinated selenocysteine is essential for...
Gladyshev, Vadim, Khangulov, Sergei V., Stadtman, Thressa C.
Nicotinic acid hydroxylase from Clostidum barkeri contains selenium in an unidentified form that is dissociated as a low molecular weight compound upon denaturation of the enzyme. Other cofactors of...
Lacourciere, Gerard M., Stadtman, Thressa C.
The selD gene from Haemophilus influenzae has been overexpressed in Escherichia coli. The expressed protein was purified to homogeneity in a four-step procedure and then carboxymethylated by reaction...
Gladyshev, Vadim N., Stadtman, Thressa C., Hatfield, Dolph L., Jeang, Kuan-Teh
It has been observed previously that plasma selenium and glutathione levels are subnormal in HIV-infected individuals, and plasma glutathione peroxidase activity is decreased. Under these conditions...
Lee, Seung-Rock, Bar-Noy, Shoshana, Kwon, Jaeyul, Levine, Rodney L., Stadtman, Thressa C., Rhee, Sue Goo
Mammalian cytosolic thioredoxin reductase (TrxR) has a redox center, consisting of Cys59/Cys64 adjacent to the flavin ring of FAD and another center consisting of Cys497/selenocysteine (SeCys)498...
Self, William T., Stadtman, Thressa C.
During purification of the selenium-dependent xanthine dehydrogenase (XDH) from Clostridium purinolyticum, another hydroxylase was uncovered that also contained selenium and exhibited similar...
Synthesis and characterization of selenotrisulfide-derivatives of lipoic acid and lipoamide
Self, William T., Tsai, Lin, Stadtman, Thressa C.
Thiol-containing compounds, such as glutathione and cysteine, react with selenite under specific conditions to form selenotrisulfides. Previous studies have focused on isolation and characterization...
Isotope exchange studies on the Escherichia coli selenophosphate synthetase mechanism
Walker, Heidi, Ferretti, James A., Stadtman, Thressa C.
Selenophosphate synthetase, the Escherichia coli selD gene product, is a 37-kDa protein that catalyzes the synthesis of selenophosphate from ATP and selenide. In the absence of selenide, ATP is...
Liu, Song-Yuan, Stadtman, Thressa C.
Mammalian selenocysteine-containing thioredoxin reductase (TR) isolated from HeLa cells and from human lung adenocarcinoma cells was separated into two major enzyme species by heparin-agarose...
Gorlatov, Sergey N., Stadtman, Thressa C.
Human thioredoxin reductase (TR) contains selenocysteine (Secys) in a redox center [cysteine (Cys)-497,Secys-498] near the C-terminus. The essential role of Secys in TR isolated from HeLa cells was...
Kim, Ick Young, Stadtman, Thressa C.
NF-κB is a major transcription factor consisting of 50(p50)- and 65(p65)-kDa proteins that controls the expression of various genes, among which are those encoding cytokines, cell adhesion...
Ogasawara, Yuki, Lacourciere, Gerard, Stadtman, Thressa C.
Selenophosphate is the active selenium-donor compound required by bacteria and mammals for the specific synthesis of Secys-tRNA, the precursor of selenocysteine in selenoenzymes. Although free...
Lacourciere, Gerard M., Levine, Rodney L., Stadtman, Thressa C.
Selenium can be metabolized for protein synthesis by two major pathways in vivo. In a specific pathway it can be inserted into polypeptide chains as the amino acid selenocysteine, as directed by the...
Mihara, Hisaaki, Kato, Shin-ichiro, Lacourciere, Gerard M., Stadtman, Thressa C., Kurihara, Tatsuo, ...
Three NifS-like proteins, IscS, CSD, and CsdB, from Escherichia coli catalyze the removal of sulfur and selenium from l-cysteine and l-selenocysteine, respectively, to form l-alanine. These enzymes...
Stadtman, E. R., Stadtman, Thressa C., Pastan, Ira, Smith, Louis Ds.
Clostridium barkeri sp. n. has been described, and its relationship to other clostridia is discussed.
Schwartz, Arnold C., Stadtman, Thressa C.
Several strains of Clostridium sticklandii, isolated from small colonies arising after treatment with 1-methyl-3-nitro-1-nitrosoguanidine, exhibited markedly depressed activities of certain catabolic...
Hardman, John K., Stadtman, Thressa C.
Hardman, John K. (National Heart Institute, National Institutes of Health, Bethesda, Md.) and Thressa C. Stadtman. Metabolism of ω-amino acids. V. Energetics of the γ-aminobutyrate fermentation by...
Ching, Wei-Mei, Wittwer, Arthur J., Tsai, Lin, Stadtman, Thressa C.
Naturally occurring seleno-tRNAs from Methanococcus vannielii account for 13-20% of the total tRNA population. Two different selenium-modified nucleosides were detected in these seleno-tRNAs. Of the...
Decomposition of Tartrates by the Coliform Bacteria
Vaughn, Reese H., Marsh, George L., Stadtman, Thressa C., Cantino, Betty C.
Tamura, Takashi, Yamamoto, Shinpei, Takahata, Muneaki, Sakaguchi, Hiromich, Tanaka, Hidehiko, Stadtman, Thressa C., ...
A labile selenium donor compound monoselenophosphate is synthesized from selenide and ATP by selenophosphate synthetase (SPS). In the present study, Sps1 and Sps2 were cloned from a cDNA library...
Characterization of potential selenium-binding proteins in the selenophosphate synthetase system
Ogasawara, Yuki, Lacourciere, Gerard M., Ishii, Kazuyuki, Stadtman, Thressa C.
Selenophosphate, an activated form of selenium that can serve as a selenium donor, is generated by the selD gene product, selenophosphate synthetase (SPS). Selenophosphate is required by several...
Patteson, Kemberly G., Trivedi, Neel, Stadtman, Thressa C.
A selenium-binding protein (SeBP) from Methanococcus vannielii was recently identified, and its gene was isolated and overexpressed in Escherichia coli [Self, W. T., Pierce, R. & Stadtman, T. C....
Selenoproteins—Tracing the Role of a Trace Element in Protein Function
Selenium is an important component of several enzymes, replacing sulfur in cysteine residues. Its discovery and significance are described in this primer.
Lacourciere, Gerard M., Stadtman, Thressa C.
The selD gene from Haemophilus influenzae has been overexpressed in Escherichia coli. The expressed protein was purified to homogeneity in a four-step procedure and then carboxymethylated by reaction...
Gladyshev, Vadim N., Stadtman, Thressa C., Hatfield, Dolph L., Jeang, Kuan-Teh
It has been observed previously that plasma selenium and glutathione levels are subnormal in HIV-infected individuals, and plasma glutathione peroxidase activity is decreased. Under these conditions...
Lee, Seung-Rock, Bar-Noy, Shoshana, Kwon, Jaeyul, Levine, Rodney L., Stadtman, Thressa C., Rhee, Sue Goo
Mammalian cytosolic thioredoxin reductase (TrxR) has a redox center, consisting of Cys59/Cys64 adjacent to the flavin ring of FAD and another center consisting of Cys497/selenocysteine (SeCys)498...
Self, William T., Stadtman, Thressa C.
During purification of the selenium-dependent xanthine dehydrogenase (XDH) from Clostridium purinolyticum, another hydroxylase was uncovered that also contained selenium and exhibited similar...
Synthesis and characterization of selenotrisulfide-derivatives of lipoic acid and lipoamide
Self, William T., Tsai, Lin, Stadtman, Thressa C.
Thiol-containing compounds, such as glutathione and cysteine, react with selenite under specific conditions to form selenotrisulfides. Previous studies have focused on isolation and characterization...
Isotope exchange studies on the Escherichia coli selenophosphate synthetase mechanism
Walker, Heidi, Ferretti, James A., Stadtman, Thressa C.
Selenophosphate synthetase, the Escherichia coli selD gene product, is a 37-kDa protein that catalyzes the synthesis of selenophosphate from ATP and selenide. In the absence of selenide, ATP is...
Liu, Song-Yuan, Stadtman, Thressa C.
Mammalian selenocysteine-containing thioredoxin reductase (TR) isolated from HeLa cells and from human lung adenocarcinoma cells was separated into two major enzyme species by heparin-agarose...
Gorlatov, Sergey N., Stadtman, Thressa C.
Human thioredoxin reductase (TR) contains selenocysteine (Secys) in a redox center [cysteine (Cys)-497,Secys-498] near the C-terminus. The essential role of Secys in TR isolated from HeLa cells was...
Kim, Ick Young, Stadtman, Thressa C.
NF-κB is a major transcription factor consisting of 50(p50)- and 65(p65)-kDa proteins that controls the expression of various genes, among which are those encoding cytokines, cell adhesion...
Ogasawara, Yuki, Lacourciere, Gerard, Stadtman, Thressa C.
Selenophosphate is the active selenium-donor compound required by bacteria and mammals for the specific synthesis of Secys-tRNA, the precursor of selenocysteine in selenoenzymes. Although free...
Lacourciere, Gerard M., Levine, Rodney L., Stadtman, Thressa C.
Selenium can be metabolized for protein synthesis by two major pathways in vivo. In a specific pathway it can be inserted into polypeptide chains as the amino acid selenocysteine, as directed by the...
Mihara, Hisaaki, Kato, Shin-ichiro, Lacourciere, Gerard M., Stadtman, Thressa C., Kurihara, Tatsuo, ...
Three NifS-like proteins, IscS, CSD, and CsdB, from Escherichia coli catalyze the removal of sulfur and selenium from l-cysteine and l-selenocysteine, respectively, to form l-alanine. These enzymes...
Stadtman, E. R., Stadtman, Thressa C., Pastan, Ira, Smith, Louis Ds.
Clostridium barkeri sp. n. has been described, and its relationship to other clostridia is discussed.
Schwartz, Arnold C., Stadtman, Thressa C.
Several strains of Clostridium sticklandii, isolated from small colonies arising after treatment with 1-methyl-3-nitro-1-nitrosoguanidine, exhibited markedly depressed activities of certain catabolic...
Hardman, John K., Stadtman, Thressa C.
Hardman, John K. (National Heart Institute, National Institutes of Health, Bethesda, Md.) and Thressa C. Stadtman. Metabolism of ω-amino acids. V. Energetics of the γ-aminobutyrate fermentation by...
Ching, Wei-Mei, Wittwer, Arthur J., Tsai, Lin, Stadtman, Thressa C.
Naturally occurring seleno-tRNAs from Methanococcus vannielii account for 13-20% of the total tRNA population. Two different selenium-modified nucleosides were detected in these seleno-tRNAs. Of the...
Decomposition of Tartrates by the Coliform Bacteria
Vaughn, Reese H., Marsh, George L., Stadtman, Thressa C., Cantino, Betty C.
Tamura, Takashi, Yamamoto, Shinpei, Takahata, Muneaki, Sakaguchi, Hiromich, Tanaka, Hidehiko, Stadtman, Thressa C., ...
A labile selenium donor compound monoselenophosphate is synthesized from selenide and ATP by selenophosphate synthetase (SPS). In the present study, Sps1 and Sps2 were cloned from a cDNA library...
Characterization of potential selenium-binding proteins in the selenophosphate synthetase system
Ogasawara, Yuki, Lacourciere, Gerard M., Ishii, Kazuyuki, Stadtman, Thressa C.
Selenophosphate, an activated form of selenium that can serve as a selenium donor, is generated by the selD gene product, selenophosphate synthetase (SPS). Selenophosphate is required by several...
Patteson, Kemberly G., Trivedi, Neel, Stadtman, Thressa C.
A selenium-binding protein (SeBP) from Methanococcus vannielii was recently identified, and its gene was isolated and overexpressed in Escherichia coli [Self, W. T., Pierce, R. & Stadtman, T. C....
Selenoproteins—Tracing the Role of a Trace Element in Protein Function
Selenium is an important component of several enzymes, replacing sulfur in cysteine residues. Its discovery and significance are described in this primer.