Hepatitis C Virus p7 Protein Is Crucial for Assembly and Release of Infectious Virions (2007)
Eike Steinmann, Francois Penin, Stephanie Kallis, Arvind H. Patel, Ralf Bartenschlager, Thomas Pietschmann
Hepatitis C virus (HCV) infection is associated with chronic liver disease and currently affects about 3% of the world population. Although much has been learned about the function of individual...
Kronke, Jan, Kittler, Ralf, Buchholz, Frank, Windisch, Marc P, Pietschmann, Thomas, Bartenschlager, Ralf, ...
Persistent infection with hepatitis C virus (HCV) is a leading cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. It has recently been shown that HCV RNA replication is...
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Foamy Virus Capsids Require the Cognate Envelope Protein for Particle Export
Pietschmann, Thomas, Heinkelein, Martin, Heldmann, Martina, Zentgraf, Hanswalter, Rethwilm, Axel, Lindemann, Dirk
Unlike other subclasses of the Retroviridae the Spumavirinae, its prototype member being the so-called human foamy virus (HFV), require the expression of the envelope (Env) glycoprotein for viral...
Pietschmann, Thomas, Zentgraf, Hanswalter, Rethwilm, Axel, Lindemann, Dirk
Foamy viruses (FVs) are highly fusogenic, and their replication induces massive syncytium formation in infected cell cultures which is believed to be mediated by expression of the envelope (Env)...
Characterization of Cell Lines Carrying Self-Replicating Hepatitis C Virus RNAs
Pietschmann, Thomas, Lohmann, Volker, Rutter, Gabriel, Kurpanek, Katharina, Bartenschlager, Ralf
Subgenomic selectable RNAs of the hepatitis C virus (HCV) have recently been shown to self-replicate to high levels in the human hepatoma cell line Huh-7 (V. Lohmann, F. Körner, J. O. Koch, U....
A Particle-Associated Glycoprotein Signal Peptide Essential for Virus Maturation and Infectivity
Lindemann, Dirk, Pietschmann, Thomas, Picard-Maureau, Marcus, Berg, Angelika, Heinkelein, Martin, Thurow, Jana, ...
Signal peptides (SP) are key determinants for targeting glycoproteins to the secretory pathway. Here we describe the involvement in particle maturation as an additional function of a viral...
Persistent and Transient Replication of Full-Length Hepatitis C Virus Genomes in Cell Culture
Pietschmann, Thomas, Lohmann, Volker, Kaul, Artur, Krieger, Nicole, Rinck, Gabriele, Rutter, Gabriel, ...
The recently developed subgenomic hepatitis C virus (HCV) replicons were limited by the fact that the sequence encoding the structural proteins was missing. Therefore, important information about a...
Bukh, Jens, Pietschmann, Thomas, Lohmann, Volker, Krieger, Nicole, Faulk, Kristina, Engle, Ronald E., ...
The development of a subgenomic replicon derived from the hepatitis C virus (HCV) strain Con1 enabled the study of viral RNA replication in Huh-7 cells. The level of replication of replicons, as well...
Replication-Competent Hybrids between Murine Leukemia Virus and Foamy Virus
Shikova-Lekova, Evelina, Lindemann, Dirk, Pietschmann, Thomas, Juretzek, Thomas, Rudolph, Wolfram, Herchenröder, Ottmar, ...
Replication-competent chimeric retroviruses constructed of members of the two subfamilies of Retroviridae, orthoretroviruses and spumaretroviruses, specifically murine leukemia viruses (MuLV) bearing...
Efficient intracellular retrotransposition of an exogenous primate retrovirus genome
Heinkelein, Martin, Pietschmann, Thomas, Jármy, Gergely, Dressler, Marco, Imrich, Horst, Thurow, Jana, ...
The foamy virus (FV) subgroup of Retroviridae reverse transcribe their RNA (pre-)genome late in the replication cycle before leaving an infected cell. We studied whether a marker gene-transducing FV...
Krönke, Jan, Kittler, Ralf, Buchholz, Frank, Windisch, Marc P., Pietschmann, Thomas, Bartenschlager, Ralf, ...
Persistent infection with hepatitis C virus (HCV) is a leading cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. It has recently been shown that HCV RNA replication is...
Schwer, Björn, Ren, Shaotang, Pietschmann, Thomas, Kartenbeck, Jürgen, Kaehlcke, Katrin, Bartenschlager, Ralf, ...
The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum (ER)....
Duda, Anja, Stange, Annett, Lüftenegger, Daniel, Stanke, Nicole, Westphal, Dana, Pietschmann, Thomas, ...
Analogous to cellular glycoproteins, viral envelope proteins contain N-terminal signal sequences responsible for targeting them to the secretory pathway. The prototype foamy virus (PFV) envelope...
Appel, Nicole, Pietschmann, Thomas, Bartenschlager, Ralf
Nonstructural protein 5A of the hepatitis C virus (HCV) is a highly phosphorylated molecule implicated in multiple interactions with the host cell and most likely involved in RNA replication. Two...
Koutsoudakis, George, Kaul, Artur, Steinmann, Eike, Kallis, Stephanie, Lohmann, Volker, Pietschmann, Thomas, ...
The lack of an efficient system to produce hepatitis C virus (HCV) particles has impeded the analysis of the HCV life cycle. Recently, we along with others demonstrated that transfection of Huh7...
Pietschmann, Thomas, Kaul, Artur, Koutsoudakis, George, Shavinskaya, Anna, Kallis, Stephanie, Steinmann, Eike, ...
Chronic liver disease caused by infection with hepatitis C virus (HCV) is an important global health problem that currently affects 170 million people. A major impediment in HCV research and drug...
Characterization of the Prototype Foamy Virus Envelope Glycoprotein Receptor-Binding Domain
Duda, Anja, Lüftenegger, Daniel, Pietschmann, Thomas, Lindemann, Dirk
The foamy virus (FV) glycoprotein precursor gp130Env undergoes a highly unusual biosynthesis, resulting in the generation of three particle-associated, mature subunits, leader peptide (LP), surface...
Foamy Virus Capsids Require the Cognate Envelope Protein for Particle Export
Pietschmann, Thomas, Heinkelein, Martin, Heldmann, Martina, Zentgraf, Hanswalter, Rethwilm, Axel, Lindemann, Dirk
Unlike other subclasses of the Retroviridae the Spumavirinae, its prototype member being the so-called human foamy virus (HFV), require the expression of the envelope (Env) glycoprotein for viral...
Pietschmann, Thomas, Zentgraf, Hanswalter, Rethwilm, Axel, Lindemann, Dirk
Foamy viruses (FVs) are highly fusogenic, and their replication induces massive syncytium formation in infected cell cultures which is believed to be mediated by expression of the envelope (Env)...
Characterization of Cell Lines Carrying Self-Replicating Hepatitis C Virus RNAs
Pietschmann, Thomas, Lohmann, Volker, Rutter, Gabriel, Kurpanek, Katharina, Bartenschlager, Ralf
Subgenomic selectable RNAs of the hepatitis C virus (HCV) have recently been shown to self-replicate to high levels in the human hepatoma cell line Huh-7 (V. Lohmann, F. Körner, J. O. Koch, U....
A Particle-Associated Glycoprotein Signal Peptide Essential for Virus Maturation and Infectivity
Lindemann, Dirk, Pietschmann, Thomas, Picard-Maureau, Marcus, Berg, Angelika, Heinkelein, Martin, Thurow, Jana, ...
Signal peptides (SP) are key determinants for targeting glycoproteins to the secretory pathway. Here we describe the involvement in particle maturation as an additional function of a viral...
Persistent and Transient Replication of Full-Length Hepatitis C Virus Genomes in Cell Culture
Pietschmann, Thomas, Lohmann, Volker, Kaul, Artur, Krieger, Nicole, Rinck, Gabriele, Rutter, Gabriel, ...
The recently developed subgenomic hepatitis C virus (HCV) replicons were limited by the fact that the sequence encoding the structural proteins was missing. Therefore, important information about a...
Bukh, Jens, Pietschmann, Thomas, Lohmann, Volker, Krieger, Nicole, Faulk, Kristina, Engle, Ronald E., ...
The development of a subgenomic replicon derived from the hepatitis C virus (HCV) strain Con1 enabled the study of viral RNA replication in Huh-7 cells. The level of replication of replicons, as well...
Replication-Competent Hybrids between Murine Leukemia Virus and Foamy Virus
Shikova-Lekova, Evelina, Lindemann, Dirk, Pietschmann, Thomas, Juretzek, Thomas, Rudolph, Wolfram, Herchenröder, Ottmar, ...
Replication-competent chimeric retroviruses constructed of members of the two subfamilies of Retroviridae, orthoretroviruses and spumaretroviruses, specifically murine leukemia viruses (MuLV) bearing...
Efficient intracellular retrotransposition of an exogenous primate retrovirus genome
Heinkelein, Martin, Pietschmann, Thomas, Jármy, Gergely, Dressler, Marco, Imrich, Horst, Thurow, Jana, ...
The foamy virus (FV) subgroup of Retroviridae reverse transcribe their RNA (pre-)genome late in the replication cycle before leaving an infected cell. We studied whether a marker gene-transducing FV...
Krönke, Jan, Kittler, Ralf, Buchholz, Frank, Windisch, Marc P., Pietschmann, Thomas, Bartenschlager, Ralf, ...
Persistent infection with hepatitis C virus (HCV) is a leading cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. It has recently been shown that HCV RNA replication is...
Schwer, Björn, Ren, Shaotang, Pietschmann, Thomas, Kartenbeck, Jürgen, Kaehlcke, Katrin, Bartenschlager, Ralf, ...
The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum (ER)....
Duda, Anja, Stange, Annett, Lüftenegger, Daniel, Stanke, Nicole, Westphal, Dana, Pietschmann, Thomas, ...
Analogous to cellular glycoproteins, viral envelope proteins contain N-terminal signal sequences responsible for targeting them to the secretory pathway. The prototype foamy virus (PFV) envelope...
Appel, Nicole, Pietschmann, Thomas, Bartenschlager, Ralf
Nonstructural protein 5A of the hepatitis C virus (HCV) is a highly phosphorylated molecule implicated in multiple interactions with the host cell and most likely involved in RNA replication. Two...
Pietschmann, Thomas, Kaul, Artur, Koutsoudakis, George, Shavinskaya, Anna, Kallis, Stephanie, Steinmann, Eike, ...
Chronic liver disease caused by infection with hepatitis C virus (HCV) is an important global health problem that currently affects 170 million people. A major impediment in HCV research and drug...
Koutsoudakis, George, Kaul, Artur, Steinmann, Eike, Kallis, Stephanie, Lohmann, Volker, Pietschmann, Thomas, ...
The lack of an efficient system to produce hepatitis C virus (HCV) particles has impeded the analysis of the HCV life cycle. Recently, we along with others demonstrated that transfection of Huh7...
Characterization of the Prototype Foamy Virus Envelope Glycoprotein Receptor-Binding Domain
Duda, Anja, Lüftenegger, Daniel, Pietschmann, Thomas, Lindemann, Dirk
The foamy virus (FV) glycoprotein precursor gp130Env undergoes a highly unusual biosynthesis, resulting in the generation of three particle-associated, mature subunits, leader peptide (LP), surface...
Koutsoudakis, George, Herrmann, Eva, Kallis, Stephanie, Bartenschlager, Ralf, Pietschmann, Thomas
Recently a cell culture model supporting the complete life cycle of the hepatitis C virus (HCV) was developed. Searching for host cell determinants involved in the HCV replication cycle, we evaluated...
Hepatitis C Virus p7 Protein Is Crucial for Assembly and Release of Infectious Virions
Steinmann, Eike, Penin, Francois, Kallis, Stephanie, Patel, Arvind H, Bartenschlager, Ralf, Pietschmann, Thomas
Hepatitis C virus (HCV) infection is associated with chronic liver disease and currently affects about 3% of the world population. Although much has been learned about the function of individual...
Schaller, Torsten, Appel, Nicole, Koutsoudakis, George, Kallis, Stephanie, Lohmann, Volker, Pietschmann, Thomas, ...
Studies of the complete hepatitis C virus (HCV) life cycle have become possible with the development of an infectious cell culture system using the genotype 2a isolate JFH-1. Taking advantage of this...
Schaller, Torsten, Appel, Nicole, Koutsoudakis, George, Kallis, Stephanie, Lohmann, Volker, Pietschmann, Thomas, ...
Efficient trans-Encapsidation of Hepatitis C Virus RNAs into Infectious Virus-Like Particles ▿
Steinmann, Eike, Brohm, Christiane, Kallis, Stephanie, Bartenschlager, Ralf, Pietschmann, Thomas
Recently, complete replication of hepatitis C virus (HCV) in tissue culture was established using the JFH1 isolate. To analyze determinants of HCV genome packaging and virion assembly, we developed a...