Thomas Ohrt

In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5

Ohrt, Thomas, Merkle, Dennis, Birkenfeld, Karin, Echeverri, Christophe J., Schwille, Petra

Two types of short double-stranded RNA molecules, namely microRNAs (miRNAs) and short interfering RNAs (siRNAs), have emerged recently as important regulators of gene expression. Although these...

In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5

Ohrt, Thomas, Merkle, Dennis, Birkenfeld, Karin, Echeverri, Christophe J., Schwille, Petra

Two types of short double-stranded RNA molecules, namely microRNAs (miRNAs) and short interfering RNAs (siRNAs), have emerged recently as important regulators of gene expression. Although these...

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells

Ohrt, Thomas, Mütze, Jörg, Staroske, Wolfgang, Weinmann, Lasse, Höck, Julia, Crell, Karin, ...

Studies of RNA interference (RNAi) provide evidence that in addition to the well-characterized cytoplasmic mechanisms, nuclear mechanisms also exist. The mechanism by which the nuclear RNA-induced...