Thierry Lonhienne

Enzyme activity determination on macromolecular substrates by isothermal titration calorimetry: application to mesophilic and psychrophilic chitinases (2001)

Lonhienne, Thierry, Baise, Etienne, Feller, Georges, Bouriotis, Vassilis, Gerday, Charles

Isothermal titration calorimetry has been applied to the determination of the kinetic parameters of chitinases (EC 3.2.1.14) by monitoring the heat released during the hydrolysis of chitin glycosidic...

Modular structure, local flexibility and cold-activity of a novel chitobiase from a psychrophilic Antarctic bacterium (2001)

Lonhienne, Thierry, Zoidakis, Jérôme, Vorgias, Constantinos E., Feller, Georges, Gerday, Charles, Bouriotis, Vassilis

The gene archb encoding for the cell-bound chitobiase from the Antarctic Gram-positive bacterium Arthrobacter sp. TAD20 was cloned and expressed in Escherichia coli in a soluble form. The mature...

Probing the role of oligomerization in the high thermal stability of Pyrococcus furiosus ornithine carbamoyltransferase by site-specific mutants (2001)

Clantin, Bernard, Tricot, Catherine, Lonhienne, Thierry, Stalon, Victor, Villeret, Vincent

The Pyrococcus furiosus ornithine carbamoyltransferase (OTCase) is extremely heat stable and maintains 50% of its catalytic activity after 60 min at 100 degreesC. The enzyme has an unusual quaternary...

Cloning, sequences, and characterization of two chitinase genes from the antarctic Arthrobacter sp strain TAD20: Isolation and partial characterization of the enzymes (2001)

Lonhienne, Thierry, Mavromatis, Konstantinos, Vorgias, Constantin E., Buchon, Laurent, Gerday, Charles, Bouriotis, Vassilis

Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA...

Cloning, Sequences, and Characterization of Two Chitinase Genes from the Antarctic Arthrobacter sp. Strain TAD20: Isolation and Partial Characterization of the Enzymes

Lonhienne, Thierry, Mavromatis, Konstantinos, Vorgias, Constantin E., Buchon, Laurent, Gerday, Charles, Bouriotis, Vassilis

Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA...

Cloning, Sequences, and Characterization of Two Chitinase Genes from the Antarctic Arthrobacter sp. Strain TAD20: Isolation and Partial Characterization of the Enzymes

Lonhienne, Thierry, Mavromatis, Konstantinos, Vorgias, Constantin E., Buchon, Laurent, Gerday, Charles, Bouriotis, Vassilis

Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA...