Teruo Ogawa

Single particle analysis of thylakoid proteins from Thermosynechococcus elongatus and Synechocystis 6803: Localization of the CupA subunit of NDH-1 (2008)

Folea, I. Mihaela, Zhang, Pengpeng, Nowaczyk, Marc M., Ogawa, Teruo, Aro, Eva-Mari, Boekema, Egbert J.

The larger protein complexes of the cyanobacterial photosynthetic membrane of Thermosynechoccus elongatus and Synechocystis 6803 were studied by single particle electron microscopy after detergent...

Structural characterization of NDH-1 complexes of Thermosynechococcus elongatus by single particle electron microscopy (2006)

Boekema, Egbert J., Aro, Eva-Mari, Ogawa, Teruo, Battchikova, Natalia, Zhang, Pengpeng, Arteni, Ana A.

The structure of the multifunctional NAD(P)H dehydrogenase type 1 (NDH-1) complexes from cyanobacteria was investigated by growing the wild type and specific ndh His-tag mutants of...

The role of an alternative sigma factor in motility and pilus formation in the cyanobacterium Synechocystis sp. strain PCC6803

Bhaya, Devaki, Watanabe, Natsu, Ogawa, Teruo, Grossman, Arthur R.

Disruption of a gene for an alternative sigma factor, designated sigF, in the freshwater, unicellular cyanobacterium Synechocystis sp. strain PCC6803 resulted in a pleiotropic phenotype. Most...

Identification of an ATP-binding cassette transporter involved in bicarbonate uptake in the cyanobacterium Synechococcus sp. strain PCC 7942

Omata, Tatsuo, Price, G. Dean, Badger, Murray R., Okamura, Masato, Gohta, Satoshi, Ogawa, Teruo

Exposure of cells of cyanobacteria (blue–green algae) grown under high-CO2 conditions to inorganic C-limitation induces transcription of particular genes and expression of high-affinity CO2 and...

Distinct constitutive and low-CO2-induced CO2 uptake systems in cyanobacteria: Genes involved and their phylogenetic relationship with homologous genes in other organisms

Shibata, Mari, Ohkawa, Hiroshi, Kaneko, Takakazu, Fukuzawa, Hideya, Tabata, Satoshi, Kaplan, Aaron, ...

Cyanobacteria possess a CO2-concentating mechanism that involves active CO2 uptake and HCO\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts}...

A Gene of Synechocystis sp. Strain PCC 6803 Encoding a Novel Iron Transporter

Katoh, Hirokazu, Grossman, Arthur R., Hagino, Natsu, Ogawa, Teruo

A mutant of Synechocystis sp. strain PCC 6803 disrupted for sll1878 exhibited greatly reduced Fe3+ transport activity. The Km value of sll1878-dependent Fe3+ transport in cells grown in iron-replete...

Genes Essential to Iron Transport in the Cyanobacterium Synechocystis sp. Strain PCC 6803

Katoh, Hirokazu, Hagino, Natsu, Grossman, Arthur R., Ogawa, Teruo

Genes encoding polypeptides of an ATP binding cassette (ABC)-type ferric iron transporter that plays a major role in iron acquisition in Synechocystis sp. strain PCC 6803 were identified. These genes...

Localization of NAD(P)H Dehydrogenase in the Cyanobacterium Synechocystis sp. Strain PCC 6803

Ohkawa, Hiroshi, Sonoda, Masatoshi, Shibata, Mari, Ogawa, Teruo

Antibodies raised against NdhH and NdhB detected these proteins in the thylakoid membrane of Synechocystis sp. strain PCC 6803, but not in a purified cytoplasmic membrane. We conclude that NAD(P)H...

Photosynthetic Electron Transport Involved in PxcA-Dependent Proton Extrusion in Synechocystis sp. Strain PCC6803: Effect of pxcA Inactivation on CO2, HCO3−, and NO3− Uptake

Sonoda, Masatoshi, Katoh, Hirokazu, Vermaas, Wim, Schmetterer, George, Ogawa, Teruo

The product of pxcA (formerly known as cotA) is involved in light-induced Na+-dependent proton extrusion. In the presence of 2,5-dimethyl-p-benzoquinone, net proton extrusion by Synechocystis sp....

Mutation of ndh Genes Leads to Inhibition of CO2 Uptake Rather than HCO3− Uptake in Synechocystis sp. Strain PCC 6803

Ohkawa, Hiroshi, Price, G. Dean, Badger, Murray R., Ogawa, Teruo

Six mutants (B1 to B6) that grew poorly in air on BG11 agar plates buffered at pH 8.0 were rescued after mutations were introduced into ndhB of wild-type (WT) Synechocystis sp. strain PCC 6803. In...

PARTIALLY PURIFIED PHOTOSYNTHETIC REACTION CENTERS FROM PLANT TISSUES*

Vernon, Leo P., Yamamoto, Harry Y., Ogawa, Teruo

The prior removal of carotenoids from photosynthetic membranes of plants by means of organic extraction allows Triton X-100 to remove and solubilize small particles that are greatly enriched in...

Genetically engineered mutant of the cyanobacterium Synechococcus PCC 7942 defective in nitrate transport

Omata, Tatsuo, Ohmori, Masayuki, Arai, Nobuyuki, Ogawa, Teruo

Nitrate-grown cells of Synechococcus PCC 7942 (Anacystis nidulans R2) contain a 45-kDa protein as a major protein in the cytoplasmic membrane but ammonium-grown cells lack it. A mutant (M45) was...

Kinetic properties of the blue-light response of stomata

Iino, Moritoshi, Ogawa, Teruo, Zeiger, Eduardo

The stomatal response to blue light was analyzed with gas-exchange techniques in Commelina communis L. leaves by using high-fluence-rate short pulses. Pulses of blue light were given under a...

Effects of Cations and Abscisic Acid on Chlorophyll a Fluorescence in Guard Cells of Vicia faba1

Ogawa, Teruo, Grantz, David, Boyer, John

The effects of cations and abscisic acid on chloroplast activity in guard cells of Vicia faba were investigated by analysis of the transient of chlorophyll a fluorescence. When epidermal strips...

Expression and Functional Roles of the Two Distinct NDH-1 Complexes and the Carbon Acquisition Complex NdhD3/NdhF3/CupA/Sll1735 in Synechocystis sp PCC 6803

Zhang, Pengpeng, Battchikova, Natalia, Jansen, Tove, Appel, Jens, Ogawa, Teruo, Aro, Eva-Mari

To investigate the (co)expression, interaction, and membrane location of multifunctional NAD(P)H dehydrogenase type 1 (NDH-1) complexes and their involvement in carbon acquisition, cyclic photosystem...

Carbonyl Sulfide: An Inhibitor of Inorganic Carbon Transport in Cyanobacteria 1

Ogawa, Teruo, Togasaki, Robert K.

Cells of a high CO2-requiring mutant (E1) and wild type of Synechococcus PCC7942 were incubated with COS in the light, then suspended in COS-free medium and their CO2 exchange was measured using an...

The Stoichiometry between CO2 and H+ Fluxes Involved in the Transport of Inorganic Carbon in Cyanobacteria 1

Ogawa, Teruo, Kaplan, Aaron

The pH of the medium during CO2 uptake into the intracellular inorganic carbon (Ci) pool of a high CO2-requiring mutant (E1) and wild type of Anacystis nidulans R2 was measured. Experiments were...

Adaptation to Low CO2 Level in a Mutant of Anacystis nidulans R2 which Requires High CO2 for Growth 1

Omata, Tatsuo, Ogawa, Teruo, Marcus, Yehouda, Friedberg, Devorah, Kaplan, Aaron

The mutant E1 of Anacystis nidulans R2 requires high CO2 concentration for growth but was able to adapt to low CO2 concentration. This was exhibited by the increased ability to accumulate inorganic...

Energization and Activation of Inorganic Carbon Uptake by Light in Cyanobacteria 1

Kaplan, Aaron, Zenvirth, Drora, Marcus, Yehouda, Omata, Tatsuo, Ogawa, Teruo

The requirement of the inorganic carbon (Ci) transport system for light in cyanobacteria was investigated in Anabaena variabilis by the filtering centrifugation technique and in a mutant (E1)...

A Mutant of Synechococcus PCC7942 Incapable of Adapting to Low CO2 Concentration 1

Ogawa, Teruo, Kaneda, Takashi, Omata, Tatsuo

Some properties of a mutant (RK1) of Synechococcus PCC7942, which requires high CO2 for growth, are described. The photosynthetic affinity for inorganic carbon (Ci) in RK1 was about 40 times lower...

Sequencing and Modification of the Gene Encoding the 42-Kilodalton Protein in the Cytoplasmic Membrane of Synechococcus PCC 7942

Omata, Tatsuo, Carlson, Thomas J., Ogawa, Teruo, Pierce, John

A 42-kilodalton cytoplasmic membrane protein is synthesized when high CO2-grown cells of Synechococcus PCC 7942 (Anacystis nidulans R2) are exposed to low CO2. The structural gene for this protein...

Biosynthesis of a 42-kD Polypeptide in the Cytoplasmic Membrane of the Cyanobacterium Anacystis nidulans Strain R2 during Adaptation to Low CO2 Concentration 1

Omata, Tatsuo, Ogawa, Teruo

When cells of Anacystis nidulans strain R2 grown under high CO2 conditions (3%) were transferred to low CO2 conditions (0.05%), their ability to accumulate inorganic carbon (Ci) increased up to 8...

Mutants of Synechocystis PCC6803 Defective in Inorganic Carbon Transport 1

Ogawa, Teruo

Eighty mutants of Synechocystis PCC6803 that require high CO2 for growth were examined with a mass spectrometer for their ability to take up CO2 in the light. Two of these mutants (type A) did not...

Identification and Characterization of the ictA/ndhL Gene Product Essential to Inorganic Carbon Transport of Synechocystis PCC6803 1

Ogawa, Teruo

The ictA gene, renamed ndhL in this paper, essential to inorganic carbon transport of Synechocystis PCC6803, was expressed in Eschericia coli as a fusion protein with glutathione S-transferase. An...

Cloning and Inactivation of a Gene Essential to Inorganic Carbon Transport of Synechocystis PCC6803 1

Ogawa, Teruo

A clone (HP-1) which transforms the high CO2-requiring mutant (RKb) of Synechocystis PCC6803 defective in inorganic carbon transport to the wild-type (WT) phenotype was isolated from a WT genomic...

Isolation, subunit composition and interaction of the NDH-1 complexes from Thermosynechococcus elongatus BP-1

Zhang, Pengpeng, Battchikova, Natalia, Paakkarinen, Virpi, Katoh, Hirokazu, Iwai, Masako, Ikeuchi, Masahiko, ...

NDH (NADH-quinone oxidoreductase)-1 complexes in cyanobacteria have specific functions in respiration and cyclic electron flow as well as in active CO2 uptake. In order to isolate NDH-1 complexes and...

The role of an alternative sigma factor in motility and pilus formation in the cyanobacterium Synechocystis sp. strain PCC6803

Bhaya, Devaki, Watanabe, Natsu, Ogawa, Teruo, Grossman, Arthur R.

Disruption of a gene for an alternative sigma factor, designated sigF, in the freshwater, unicellular cyanobacterium Synechocystis sp. strain PCC6803 resulted in a pleiotropic phenotype. Most...

Identification of an ATP-binding cassette transporter involved in bicarbonate uptake in the cyanobacterium Synechococcus sp. strain PCC 7942

Omata, Tatsuo, Price, G. Dean, Badger, Murray R., Okamura, Masato, Gohta, Satoshi, Ogawa, Teruo

Exposure of cells of cyanobacteria (blue–green algae) grown under high-CO2 conditions to inorganic C-limitation induces transcription of particular genes and expression of high-affinity CO2 and...

Distinct constitutive and low-CO2-induced CO2 uptake systems in cyanobacteria: Genes involved and their phylogenetic relationship with homologous genes in other organisms

Shibata, Mari, Ohkawa, Hiroshi, Kaneko, Takakazu, Fukuzawa, Hideya, Tabata, Satoshi, Kaplan, Aaron, ...

Cyanobacteria possess a CO2-concentating mechanism that involves active CO2 uptake and HCO\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts}...

A Gene of Synechocystis sp. Strain PCC 6803 Encoding a Novel Iron Transporter

Katoh, Hirokazu, Grossman, Arthur R., Hagino, Natsu, Ogawa, Teruo

A mutant of Synechocystis sp. strain PCC 6803 disrupted for sll1878 exhibited greatly reduced Fe3+ transport activity. The Km value of sll1878-dependent Fe3+ transport in cells grown in iron-replete...

Genes Essential to Iron Transport in the Cyanobacterium Synechocystis sp. Strain PCC 6803

Katoh, Hirokazu, Hagino, Natsu, Grossman, Arthur R., Ogawa, Teruo

Genes encoding polypeptides of an ATP binding cassette (ABC)-type ferric iron transporter that plays a major role in iron acquisition in Synechocystis sp. strain PCC 6803 were identified. These genes...

Localization of NAD(P)H Dehydrogenase in the Cyanobacterium Synechocystis sp. Strain PCC 6803

Ohkawa, Hiroshi, Sonoda, Masatoshi, Shibata, Mari, Ogawa, Teruo

Antibodies raised against NdhH and NdhB detected these proteins in the thylakoid membrane of Synechocystis sp. strain PCC 6803, but not in a purified cytoplasmic membrane. We conclude that NAD(P)H...

Photosynthetic Electron Transport Involved in PxcA-Dependent Proton Extrusion in Synechocystis sp. Strain PCC6803: Effect of pxcA Inactivation on CO2, HCO3−, and NO3− Uptake

Sonoda, Masatoshi, Katoh, Hirokazu, Vermaas, Wim, Schmetterer, George, Ogawa, Teruo

The product of pxcA (formerly known as cotA) is involved in light-induced Na+-dependent proton extrusion. In the presence of 2,5-dimethyl-p-benzoquinone, net proton extrusion by Synechocystis sp....

Mutation of ndh Genes Leads to Inhibition of CO2 Uptake Rather than HCO3− Uptake in Synechocystis sp. Strain PCC 6803

Ohkawa, Hiroshi, Price, G. Dean, Badger, Murray R., Ogawa, Teruo

Six mutants (B1 to B6) that grew poorly in air on BG11 agar plates buffered at pH 8.0 were rescued after mutations were introduced into ndhB of wild-type (WT) Synechocystis sp. strain PCC 6803. In...

PARTIALLY PURIFIED PHOTOSYNTHETIC REACTION CENTERS FROM PLANT TISSUES*

Vernon, Leo P., Yamamoto, Harry Y., Ogawa, Teruo

The prior removal of carotenoids from photosynthetic membranes of plants by means of organic extraction allows Triton X-100 to remove and solubilize small particles that are greatly enriched in...

Genetically engineered mutant of the cyanobacterium Synechococcus PCC 7942 defective in nitrate transport

Omata, Tatsuo, Ohmori, Masayuki, Arai, Nobuyuki, Ogawa, Teruo

Nitrate-grown cells of Synechococcus PCC 7942 (Anacystis nidulans R2) contain a 45-kDa protein as a major protein in the cytoplasmic membrane but ammonium-grown cells lack it. A mutant (M45) was...

Kinetic properties of the blue-light response of stomata

Iino, Moritoshi, Ogawa, Teruo, Zeiger, Eduardo

The stomatal response to blue light was analyzed with gas-exchange techniques in Commelina communis L. leaves by using high-fluence-rate short pulses. Pulses of blue light were given under a...

Effects of Cations and Abscisic Acid on Chlorophyll a Fluorescence in Guard Cells of Vicia faba1

Ogawa, Teruo, Grantz, David, Boyer, John

The effects of cations and abscisic acid on chloroplast activity in guard cells of Vicia faba were investigated by analysis of the transient of chlorophyll a fluorescence. When epidermal strips...

Expression and Functional Roles of the Two Distinct NDH-1 Complexes and the Carbon Acquisition Complex NdhD3/NdhF3/CupA/Sll1735 in Synechocystis sp PCC 6803

Zhang, Pengpeng, Battchikova, Natalia, Jansen, Tove, Appel, Jens, Ogawa, Teruo, Aro, Eva-Mari

To investigate the (co)expression, interaction, and membrane location of multifunctional NAD(P)H dehydrogenase type 1 (NDH-1) complexes and their involvement in carbon acquisition, cyclic photosystem...

Carbonyl Sulfide: An Inhibitor of Inorganic Carbon Transport in Cyanobacteria 1

Ogawa, Teruo, Togasaki, Robert K.

Cells of a high CO2-requiring mutant (E1) and wild type of Synechococcus PCC7942 were incubated with COS in the light, then suspended in COS-free medium and their CO2 exchange was measured using an...

The Stoichiometry between CO2 and H+ Fluxes Involved in the Transport of Inorganic Carbon in Cyanobacteria 1

Ogawa, Teruo, Kaplan, Aaron

The pH of the medium during CO2 uptake into the intracellular inorganic carbon (Ci) pool of a high CO2-requiring mutant (E1) and wild type of Anacystis nidulans R2 was measured. Experiments were...

Adaptation to Low CO2 Level in a Mutant of Anacystis nidulans R2 which Requires High CO2 for Growth 1

Omata, Tatsuo, Ogawa, Teruo, Marcus, Yehouda, Friedberg, Devorah, Kaplan, Aaron

The mutant E1 of Anacystis nidulans R2 requires high CO2 concentration for growth but was able to adapt to low CO2 concentration. This was exhibited by the increased ability to accumulate inorganic...

Energization and Activation of Inorganic Carbon Uptake by Light in Cyanobacteria 1

Kaplan, Aaron, Zenvirth, Drora, Marcus, Yehouda, Omata, Tatsuo, Ogawa, Teruo

The requirement of the inorganic carbon (Ci) transport system for light in cyanobacteria was investigated in Anabaena variabilis by the filtering centrifugation technique and in a mutant (E1)...

A Mutant of Synechococcus PCC7942 Incapable of Adapting to Low CO2 Concentration 1

Ogawa, Teruo, Kaneda, Takashi, Omata, Tatsuo

Some properties of a mutant (RK1) of Synechococcus PCC7942, which requires high CO2 for growth, are described. The photosynthetic affinity for inorganic carbon (Ci) in RK1 was about 40 times lower...

Sequencing and Modification of the Gene Encoding the 42-Kilodalton Protein in the Cytoplasmic Membrane of Synechococcus PCC 7942

Omata, Tatsuo, Carlson, Thomas J., Ogawa, Teruo, Pierce, John

A 42-kilodalton cytoplasmic membrane protein is synthesized when high CO2-grown cells of Synechococcus PCC 7942 (Anacystis nidulans R2) are exposed to low CO2. The structural gene for this protein...

Biosynthesis of a 42-kD Polypeptide in the Cytoplasmic Membrane of the Cyanobacterium Anacystis nidulans Strain R2 during Adaptation to Low CO2 Concentration 1

Omata, Tatsuo, Ogawa, Teruo

When cells of Anacystis nidulans strain R2 grown under high CO2 conditions (3%) were transferred to low CO2 conditions (0.05%), their ability to accumulate inorganic carbon (Ci) increased up to 8...

Mutants of Synechocystis PCC6803 Defective in Inorganic Carbon Transport 1

Ogawa, Teruo

Eighty mutants of Synechocystis PCC6803 that require high CO2 for growth were examined with a mass spectrometer for their ability to take up CO2 in the light. Two of these mutants (type A) did not...

Identification and Characterization of the ictA/ndhL Gene Product Essential to Inorganic Carbon Transport of Synechocystis PCC6803 1

Ogawa, Teruo

The ictA gene, renamed ndhL in this paper, essential to inorganic carbon transport of Synechocystis PCC6803, was expressed in Eschericia coli as a fusion protein with glutathione S-transferase. An...

Cloning and Inactivation of a Gene Essential to Inorganic Carbon Transport of Synechocystis PCC6803 1

Ogawa, Teruo

A clone (HP-1) which transforms the high CO2-requiring mutant (RKb) of Synechocystis PCC6803 defective in inorganic carbon transport to the wild-type (WT) phenotype was isolated from a WT genomic...

Isolation, subunit composition and interaction of the NDH-1 complexes from Thermosynechococcus elongatus BP-1

Zhang, Pengpeng, Battchikova, Natalia, Paakkarinen, Virpi, Katoh, Hirokazu, Iwai, Masako, Ikeuchi, Masahiko, ...

NDH (NADH-quinone oxidoreductase)-1 complexes in cyanobacteria have specific functions in respiration and cyclic electron flow as well as in active CO2 uptake. In order to isolate NDH-1 complexes and...

The Plant-Like C2 Glycolate Cycle and the Bacterial-Like Glycerate Pathway Cooperate in Phosphoglycolate Metabolism in Cyanobacteria1

Eisenhut, Marion, Kahlon, Shira, Hasse, Dirk, Ewald, Ralph, Lieman-Hurwitz, Judy, Ogawa, Teruo, ...

The occurrence of a photorespiratory 2-phosphoglycolate metabolism in cyanobacteria is not clear. In the genome of the cyanobacterium Synechocystis sp. strain PCC 6803, we have identified open...