Umeda, Mamoru, Yasuda, Tatsuji
We have already developed the liposome immune lysis assay (LILA) for the determination of C-reactive protein (CRP) by employing an inhibition method and a sandwich method. We herein report a new LILA...
Zhang, Daxian, Yasuda, Tatsuji, Yu, Yingyan, Okada, Shigeru
A carboxyfluorescein (CF)-enveloping soybean phosphatidylcholine liposome was used as a model of physicochemical damage of biomembranes. The liposomes were exposed to a metal-chelate complex [2 mM of...
Yonei, Taiji, Watarai, Shinobu, Okada, Yoshio, Yasuda, Tatsuji, Tsuji, Takao
Monoclonal antibodies were raised against urine proteins from diabetic patients. An antibody, YO-2, stained three protein bands with apparent molecular weights of 66, 49, and 36 kDa. These bands were...
Zhao, Dan-Dan, Watarai, Shinobu, Lee, Jin-tae, Kouchi, Shuuichi, Ohmori, Hitishi, Yasuda, Tatsuji
We compared the transfection efficiency of four types of positively charged liposomes composed of (i) N-(α-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), dilauroylphosphatidylcholine...
Availability of Liposomes as Drug Carriers to the Brain (2007)
Kobayashi, Kazuko, Han, Mei, Watarai, Shinobu, Yasuda, Tatsuji
Phospholipid vesicles, also known as liposomes, were examined for their ability to act as a drug carrier to the brain. 9-Amino-1,2,3,4-tetrahydroacridine (THA), a centrally acting...
Sensitive detection of ganglioside GD3 on the cell surface using liposome immune lysis assay. (2007)
Kobayashi, Kazuko, Watarai, Shinobu, Yasuda, Tatsuji
We developed a sensitive method for detection of glycosphingolipid (GSL) antigen(s) on the cell surface. As a model of GSL antigen, ganglioside GD3 was used. An IgM monoclonal antibody (DSG-1)...
ARIX and PHOX2B polymorphisms in patients with congenital superior oblique muscle palsy. (2006)
Jiang, Yan, Matsuo, Toshihiko, Fujiwara, Hirotake, Hasebe, Satoshi, Ohtsuki, Hiroshi, Yasuda, Tatsuji
To identify ARIX gene and PHOX2B gene polymorphisms in patients with congenital superior oblique muscle palsy, 3 exons of the ARIX gene and PHOX2B gene were sequenced by genomic DNA amplification...
Akane Kondo, Junko Inagaki, Kazuko Kobayashi, Hideo Tsukamoto, Daisuke Yamamoto, Mikiya Nakatsuka, ...
Laminin-1 is a structural glycoprotein that forms an integral part of the scaffolding of basement membranes, and plays an important role during embryonic development. We have recently demonstrated a...
Phosphatidylinositol 4,5-bisphosphate stimulates vesicle formation from liposomes by brain cytosol
Kinuta, Masahiro, Yamada, Hiroshi, Abe, Tadashi, Watanabe, Masami, Li, Shun-Ai, Kamitani, Akihiro, ...
As a step toward the elucidation of mechanisms in vesicle budding, a cell-free assay that measures cytosol-induced vesicle generation from liposomes was established. This assay then was used to...
The stimulatory action of amphiphysin on dynamin function is dependent on lipid bilayer curvature
Yoshida, Yumi, Kinuta, Masahiro, Abe, Tadashi, Liang, Shuang, Araki, Kenta, Cremona, Ottavio, ...
Amphiphysin is a major dynamin-binding partner at the synapse; however, its function in fission is unclear. Incubation of large unilamellar liposomes with mice brain cytosol led to massive formation...
Phosphatidylinositol 4,5-bisphosphate stimulates vesicle formation from liposomes by brain cytosol
Kinuta, Masahiro, Yamada, Hiroshi, Abe, Tadashi, Watanabe, Masami, Li, Shun-Ai, Kamitani, Akihiro, ...
As a step toward the elucidation of mechanisms in vesicle budding, a cell-free assay that measures cytosol-induced vesicle generation from liposomes was established. This assay then was used to...
The stimulatory action of amphiphysin on dynamin function is dependent on lipid bilayer curvature
Yoshida, Yumi, Kinuta, Masahiro, Abe, Tadashi, Liang, Shuang, Araki, Kenta, Cremona, Ottavio, ...
Amphiphysin is a major dynamin-binding partner at the synapse; however, its function in fission is unclear. Incubation of large unilamellar liposomes with mice brain cytosol led to massive formation...
Kondo, Akane, Inagaki, Junko, Kobayashi, Kazuko, Tsukamoto, Hideo, Yamamoto, Daisuke, Nakatsuka, Mikiya, ...
Laminin-1 is a structural glycoprotein that forms an integral part of the scaffolding of basement membranes, and plays an important role during embryonic development. We have recently demonstrated a...