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Nucleolar Factors Direct the 2′-O-Ribose Methylation and Pseudouridylation of U6 Spliceosomal RNA
Ganot, Philippe, Jády, Beáta E., Bortolin, Marie-Line, Darzacq, Xavier, Kiss, Tamás
The nucleolus has long been known as a functionally highly specialized subnuclear compartment where synthesis, posttranscriptional modification, and processing of cytoplasmic rRNAs take place. In...
Processing of Intron-Encoded Box C/D Small Nucleolar RNAs Lacking a 5′,3′-Terminal Stem Structure
The C and D box-containing (box C/D) small nucleolar RNAs (snoRNAs) function in the nucleolytic processing and 2′-O-methylation of precursor rRNA. In vertebrates, most box C/D snoRNAs are processed...
Cell Cycle-dependent Recruitment of Telomerase RNA and Cajal Bodies to Human Telomeres
Jády, Beáta E., Richard, Patricia, Bertrand, Edouard, Kiss, Tamás
Telomerase is a ribonucleoprotein enzyme that counteracts replicative telomere erosion by adding telomeric sequence repeats onto chromosome ends. Despite its well-established role in telomere...
Analysis of the structure of human telomerase RNA in vivo
Antal, Mária, Boros, Éva, Solymosy, Ferenc, Kiss, Tamás
Telomerase is a ribonucleoprotein reverse transcriptase that synthesises telomeric DNA. The RNA component of telomerase acts as a template for telomere synthesis and binds the reverse transcriptase....
In eukaryotic cells, the site-specific 2′-O-ribose methy-lation of ribosomal RNAs (rRNAs) and the U6 spliceosomal small nuclear RNA (snRNA) is directed by small nucleolar RNAs (snoRNAs). The C and...
Darzacq, Xavier, Jády, Beáta E., Verheggen, Céline, Kiss, Arnold M., Bertrand, Edouard, Kiss, Tamás
Cajal (coiled) bodies are conserved subnuclear organelles that are present in the nucleoplasm of both animal and plant cells. Although Cajal bodies were first described nearly 100 years ago, their...
In eukaryotes, two distinct classes of small nucleolar RNAs (snoRNAs), namely the fibrillarin-associated box C/D snoRNAs and the Gar1p-associated box H/ACA snoRNAs, direct the site-specific...
A Cajal body-specific pseudouridylation guide RNA is composed of two box H/ACA snoRNA-like domains
Kiss, Arnold M., Jády, Beáta E., Darzacq, Xavier, Verheggen, Céline, Bertrand, Edouard, Kiss, Tamás
Site-specific post-transcriptional conversion of uridines to pseudouridine in ribosomal RNAs and small nuclear RNAs (snRNAs) is directed by guide RNAs which possess the conserved box H and ACA...
Jády, Beáta E., Darzacq, Xavier, Tucker, Karen E., Matera, A.Gregory, Bertrand, Edouard, Kiss, Tamás
Biogenesis of functional spliceosomal small nuclear RNAs (snRNAs) includes the post-transcriptional covalent modification of numerous internal nucleotides. We have recently demonstrated that...
A common sequence motif determines the Cajal body-specific localization of box H/ACA scaRNAs
Richard, Patricia, Darzacq, Xavier, Bertrand, Edouard, Jády, Beáta E., Verheggen, Céline, Kiss, Tamás
Post-transcriptional synthesis of 2′-O-methylated nucleotides and pseudouridines in Sm spliceosomal small nuclear RNAs takes place in the nucleoplasmic Cajal bodies and it is directed by guide RNAs...
Atzorn, Vera, Fragapane, Paola, Kiss, Tamás
Saccharomyces cerevisiae snR30 is an essential box H/ACA small nucleolar RNA (snoRNA) required for the processing of 18S rRNA. Here, we show that the previously characterized human, reptilian,...
Human Box H/ACA Pseudouridylation Guide RNA Machinery†
Kiss, Arnold M., Jády, Beáta E., Bertrand, Edouard, Kiss, Tamás
Pseudouridine, the most abundant modified nucleoside in RNA, is synthesized by posttranscriptional isomerization of uridines. In eukaryotic RNAs, site-specific synthesis of pseudouridines is directed...
Egloff, Sylvain, Van Herreweghe, Elodie, Kiss, Tamás
The positive transcription elongation factor b (P-TEFb), a complex of Cdk9 and cyclin T1/T2, stimulates transcription by phosphorylating RNA polymerase II. The 7SK small nuclear RNA, in cooperation...
Richard, Patricia, Kiss, Arnold M., Darzacq, Xavier, Kiss, Tamás
Processing from pre-mRNA introns is a widespread mechanism to generate human box C/D and H/ACA snoRNAs. Recent studies revealed that an optimal position relative to the 3′ splice site is important...
Nucleolar Factors Direct the 2′-O-Ribose Methylation and Pseudouridylation of U6 Spliceosomal RNA
Ganot, Philippe, Jády, Beáta E., Bortolin, Marie-Line, Darzacq, Xavier, Kiss, Tamás
The nucleolus has long been known as a functionally highly specialized subnuclear compartment where synthesis, posttranscriptional modification, and processing of cytoplasmic rRNAs take place. In...
Processing of Intron-Encoded Box C/D Small Nucleolar RNAs Lacking a 5′,3′-Terminal Stem Structure
The C and D box-containing (box C/D) small nucleolar RNAs (snoRNAs) function in the nucleolytic processing and 2′-O-methylation of precursor rRNA. In vertebrates, most box C/D snoRNAs are processed...
Analysis of the structure of human telomerase RNA in vivo
Antal, Mária, Boros, Éva, Solymosy, Ferenc, Kiss, Tamás
Telomerase is a ribonucleoprotein reverse transcriptase that synthesises telomeric DNA. The RNA component of telomerase acts as a template for telomere synthesis and binds the reverse transcriptase....
In eukaryotic cells, the site-specific 2′-O-ribose methy-lation of ribosomal RNAs (rRNAs) and the U6 spliceosomal small nuclear RNA (snRNA) is directed by small nucleolar RNAs (snoRNAs). The C and...
Darzacq, Xavier, Jády, Beáta E., Verheggen, Céline, Kiss, Arnold M., Bertrand, Edouard, Kiss, Tamás
Cajal (coiled) bodies are conserved subnuclear organelles that are present in the nucleoplasm of both animal and plant cells. Although Cajal bodies were first described nearly 100 years ago, their...
In eukaryotes, two distinct classes of small nucleolar RNAs (snoRNAs), namely the fibrillarin-associated box C/D snoRNAs and the Gar1p-associated box H/ACA snoRNAs, direct the site-specific...
A Cajal body-specific pseudouridylation guide RNA is composed of two box H/ACA snoRNA-like domains
Kiss, Arnold M., Jády, Beáta E., Darzacq, Xavier, Verheggen, Céline, Bertrand, Edouard, Kiss, Tamás
Site-specific post-transcriptional conversion of uridines to pseudouridine in ribosomal RNAs and small nuclear RNAs (snRNAs) is directed by guide RNAs which possess the conserved box H and ACA...
Jády, Beáta E., Darzacq, Xavier, Tucker, Karen E., Matera, A.Gregory, Bertrand, Edouard, Kiss, Tamás
Biogenesis of functional spliceosomal small nuclear RNAs (snRNAs) includes the post-transcriptional covalent modification of numerous internal nucleotides. We have recently demonstrated that...
A common sequence motif determines the Cajal body-specific localization of box H/ACA scaRNAs
Richard, Patricia, Darzacq, Xavier, Bertrand, Edouard, Jády, Beáta E., Verheggen, Céline, Kiss, Tamás
Post-transcriptional synthesis of 2′-O-methylated nucleotides and pseudouridines in Sm spliceosomal small nuclear RNAs takes place in the nucleoplasmic Cajal bodies and it is directed by guide RNAs...
Atzorn, Vera, Fragapane, Paola, Kiss, Tamás
Saccharomyces cerevisiae snR30 is an essential box H/ACA small nucleolar RNA (snoRNA) required for the processing of 18S rRNA. Here, we show that the previously characterized human, reptilian,...
Human Box H/ACA Pseudouridylation Guide RNA Machinery†
Kiss, Arnold M., Jády, Beáta E., Bertrand, Edouard, Kiss, Tamás
Pseudouridine, the most abundant modified nucleoside in RNA, is synthesized by posttranscriptional isomerization of uridines. In eukaryotic RNAs, site-specific synthesis of pseudouridines is directed...
Egloff, Sylvain, Van Herreweghe, Elodie, Kiss, Tamás
The positive transcription elongation factor b (P-TEFb), a complex of Cdk9 and cyclin T1/T2, stimulates transcription by phosphorylating RNA polymerase II. The 7SK small nuclear RNA, in cooperation...
Cell Cycle-dependent Recruitment of Telomerase RNA and Cajal Bodies to Human Telomeres
Jády, Beáta E., Richard, Patricia, Bertrand, Edouard, Kiss, Tamás
Telomerase is a ribonucleoprotein enzyme that counteracts replicative telomere erosion by adding telomeric sequence repeats onto chromosome ends. Despite its well-established role in telomere...
Richard, Patricia, Kiss, Arnold M., Darzacq, Xavier, Kiss, Tamás
Processing from pre-mRNA introns is a widespread mechanism to generate human box C/D and H/ACA snoRNAs. Recent studies revealed that an optimal position relative to the 3′ splice site is important...
Human telomerase RNA and box H/ACA scaRNAs share a common Cajal body–specific localization signal
Jády, Beáta E., Bertrand, Edouard, Kiss, Tamás
Telomerase is a ribonucleoprotein reverse transcriptase that uses its RNA component as a template for synthesis of telomeric DNA repeats at the ends of linear eukaryotic chromosomes. Here,...
Complex Formation Between Ca(II), Mg(II), Al(III) Ions and Salicylglycine
Kilyén, Melinda, Labádi, Lmre, Tombácz, Etelka, Kiss, Tamás
For modelling the interactions of proteins/peptides with hard metal ions the complex formation of salicylglycine (SalGly) with Ca(II), Mg(ll) and AI(III) ions was studied in aqueous solution using...
Dynamic remodelling of human 7SK snRNP controls the nuclear level of active P-TEFb
Van Herreweghe, Elodie, Egloff, Sylvain, Goiffon, Isabelle, Jády, Beáta E, Froment, Carine, Monsarrat, Bernard, ...
The 7SK small nuclear RNA (snRNA) regulates RNA polymerase II transcription elongation by controlling the protein kinase activity of the positive transcription elongation factor b (P-TEFb). In...
The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery
Boulon, Séverine, Marmier-Gourrier, Nathalie, Pradet-Balade, Bérengère, Wurth, Laurence, Verheggen, Céline, Jády, Beáta E., ...
RNA-binding proteins of the L7Ae family are at the heart of many essential ribonucleoproteins (RNPs), including box C/D and H/ACA small nucleolar RNPs, U4 small nuclear RNP, telomerase, and messenger...