Generation of medaka gene knockout models by target-selected mutagenesis (2006)
Taniguchi, Yoshihito, Takeda, Shunichi, Furutani-Seiki, Makoto, Kamei, Yasuhiro, Todo, Takeshi, Sasado, Takao, ...
Abstract We have established a reverse genetics approach for the routine generation of medaka ( Oryzias latipes ) gene knockouts. A cryopreserved library of N -ethyl- N -nitrosourea (ENU) mutagenized...
Summary Cryptochromes are photoreceptors that regulate entrainment by light of the circadian clock in plants and animals. They also act as integral parts of the central circadian oscillator in animal...
Todo, Takeshi, Roark, Margaret, Raghavan, K. Vijay, Mayeda, Carol, Meyerowitz, Elliot
The Sgs-3 gene of Drosophila melanogaster exhibits a tightly regulated pattern of expression governed by two functionally equivalent elements within 1 kb of the gene, each of which is sufficient to...
Differential regulation of mammalian Period genes and circadian rhythmicity by cryptochromes 1 and 2
Vitaterna, Martha Hotz, Selby, Christopher P., Todo, Takeshi, Niwa, Hitoshi, Thompson, Carol, Fruechte, Ethan M., ...
Cryptochromes regulate the circadian clock in animals and plants. Humans and mice have two cryptochrome (Cry) genes. A previous study showed that mice lacking the Cry2 gene had reduced sensitivity to...
Hitomi, Kenichi, Okamoto, Kazuhisa, Daiyasu, Hiromi, Miyashita, Hiroshi, Iwai, Shigenori, Toh, Hiroyuki, ...
Photolyase is a DNA repair enzyme that reverses UV-induced photoproducts in DNA in a light-dependent manner. Recently, photolyase homologs were identified in higher eukaryotes. These homologs, termed...
Weber, Stefan, Kay, Christopher W. M., Mögling, Heike, Möbius, Klaus, Hitomi, Kenichi, Todo, Takeshi
The light-induced electron transfer reaction of flavin cofactor photoactivation in Xenopus laevis (6–4) photolyase has been studied by continuous-wave and time-resolved electron paramagnetic...
New role of zCRY and zPER2 as regulators of sub-cellular distributions of zCLOCK and zBMAL proteins
Hirayama, Jun, Fukuda, Itsuki, Ishikawa, Tomoko, Kobayashi, Yuri, Todo, Takeshi
The core oscillator that generates circadian rhythm in eukaryotes consists of transcription/translation-based autoregulatory feedback loops by which clock gene products negatively regulate their own...
Sakoyama, Yasuhiko, Todo, Takeshi, Ishiwa-Chigusa, Sadao, Honjo, Tasuku, Kondo, Sohei
Several DNA sequences with homology to the complete 2.9-kilobase (kb) P element from a P strain in the United States were isolated and characterized from two Drosophila melanogaster strains collected...
Cryptochromes are photoreceptors that regulate entrainment of the circadian clock by light in plants and animals. They are related to DNA photolyases and have similar three-dimensional structures,...
Masuki, Shizue, Todo, Takeshi, Nakano, Yasushi, Okamura, Hitoshi, Nose, Hiroshi
To reveal the role of clock genes in generating the circadian rhythm of baroreflexes, we continuously measured mean arterial pressure and baroreflex sensitivity in free-moving normal wild-type mice,...
Yamamoto, Junpei, Hitomi, Kenichi, Todo, Takeshi, Iwai, Shigenori
The pyrimidine(6–4)pyrimidone photoproduct, a major UV lesion formed between adjacent pyrimidine bases, is transformed to its Dewar valence isomer upon exposure to UVA/UVB light. We have...
Differential regulation of mammalian Period genes and circadian rhythmicity by cryptochromes 1 and 2
Vitaterna, Martha Hotz, Selby, Christopher P., Todo, Takeshi, Niwa, Hitoshi, Thompson, Carol, Fruechte, Ethan M., ...
Cryptochromes regulate the circadian clock in animals and plants. Humans and mice have two cryptochrome (Cry) genes. A previous study showed that mice lacking the Cry2 gene had reduced sensitivity to...
Hitomi, Kenichi, Okamoto, Kazuhisa, Daiyasu, Hiromi, Miyashita, Hiroshi, Iwai, Shigenori, Toh, Hiroyuki, ...
Photolyase is a DNA repair enzyme that reverses UV-induced photoproducts in DNA in a light-dependent manner. Recently, photolyase homologs were identified in higher eukaryotes. These homologs, termed...
Weber, Stefan, Kay, Christopher W. M., Mögling, Heike, Möbius, Klaus, Hitomi, Kenichi, Todo, Takeshi
The light-induced electron transfer reaction of flavin cofactor photoactivation in Xenopus laevis (6–4) photolyase has been studied by continuous-wave and time-resolved electron paramagnetic...
New role of zCRY and zPER2 as regulators of sub-cellular distributions of zCLOCK and zBMAL proteins
Hirayama, Jun, Fukuda, Itsuki, Ishikawa, Tomoko, Kobayashi, Yuri, Todo, Takeshi
The core oscillator that generates circadian rhythm in eukaryotes consists of transcription/translation-based autoregulatory feedback loops by which clock gene products negatively regulate their own...
Sakoyama, Yasuhiko, Todo, Takeshi, Ishiwa-Chigusa, Sadao, Honjo, Tasuku, Kondo, Sohei
Several DNA sequences with homology to the complete 2.9-kilobase (kb) P element from a P strain in the United States were isolated and characterized from two Drosophila melanogaster strains collected...
Cryptochromes are photoreceptors that regulate entrainment of the circadian clock by light in plants and animals. They are related to DNA photolyases and have similar three-dimensional structures,...
Masuki, Shizue, Todo, Takeshi, Nakano, Yasushi, Okamura, Hitoshi, Nose, Hiroshi
To reveal the role of clock genes in generating the circadian rhythm of baroreflexes, we continuously measured mean arterial pressure and baroreflex sensitivity in free-moving normal wild-type mice,...
Yamamoto, Junpei, Hitomi, Kenichi, Todo, Takeshi, Iwai, Shigenori
The pyrimidine(6–4)pyrimidone photoproduct, a major UV lesion formed between adjacent pyrimidine bases, is transformed to its Dewar valence isomer upon exposure to UVA/UVB light. We have...
Generation of medaka gene knockout models by target-selected mutagenesis
Taniguchi, Yoshihito, Takeda, Shunichi, Furutani-Seiki, Makoto, Kamei, Yasuhiro, Todo, Takeshi, Sasado, Takao, ...
A reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts is described and applied to create a cryopreserved resource containing knockouts for most medaka...