Takatsugu Kobayashi

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells

Sakamoto, Kensaku, Hayashi, Akiko, Sakamoto, Ayako, Kiga, Daisuke, Nakayama, Hiroshi, Soma, Akiko, ...

A suppressor tRNATyr and mutant tyrosyl-tRNA synthetase (TyrRS) pair was developed to incorporate 3-iodo-l-tyrosine into proteins in mammalian cells. First, the Escherichia coli suppressor tRNATyr...

The Escherichia coli argU10(Ts) Phenotype Is Caused by a Reduction in the Cellular Level of the argU tRNA for the Rare Codons AGA and AGG

Sakamoto, Kensaku, Ishimaru, Satoshi, Kobayashi, Takatsugu, Walker, James R., Yokoyama, Shigeyuki

The Escherichia coli argU10(Ts) mutation in the argU gene, encoding the minor tRNAArg species for the rare codons AGA and AGG, causes pleiotropic defects, including growth inhibition at high...

Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion

Kobayashi, Takatsugu, Sakamoto, Kensaku, Takimura, Tetsuo, Sekine, Ryo, Vincent, Kelly, Kamata, Kenji, ...

The genetic code in a eukaryotic system has been expanded by the engineering of Escherichia coli tyrosyl-tRNA synthetase (TyrRS) with the Y37V and Q195C mutations (37V195C), which specifically...

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells

Sakamoto, Kensaku, Hayashi, Akiko, Sakamoto, Ayako, Kiga, Daisuke, Nakayama, Hiroshi, Soma, Akiko, ...

A suppressor tRNATyr and mutant tyrosyl-tRNA synthetase (TyrRS) pair was developed to incorporate 3-iodo-l-tyrosine into proteins in mammalian cells. First, the Escherichia coli suppressor tRNATyr...

The Escherichia coli argU10(Ts) Phenotype Is Caused by a Reduction in the Cellular Level of the argU tRNA for the Rare Codons AGA and AGG

Sakamoto, Kensaku, Ishimaru, Satoshi, Kobayashi, Takatsugu, Walker, James R., Yokoyama, Shigeyuki

The Escherichia coli argU10(Ts) mutation in the argU gene, encoding the minor tRNAArg species for the rare codons AGA and AGG, causes pleiotropic defects, including growth inhibition at high...

Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion

Kobayashi, Takatsugu, Sakamoto, Kensaku, Takimura, Tetsuo, Sekine, Ryo, Vincent, Kelly, Kamata, Kenji, ...

The genetic code in a eukaryotic system has been expanded by the engineering of Escherichia coli tyrosyl-tRNA synthetase (TyrRS) with the Y37V and Q195C mutations (37V195C), which specifically...