Tadashi Wada

Drosophila blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway (2008)

Agawa, Yasuo, Sarhan, Moustafa, Kageyama, Yuji, Akagi, Kazutaka, Takai, Masayoshi, Hashiyama, Kazuya, ...

Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-fl is expressed in a temporally regulated manner following pulses of ecdysteroid...

Tat-SF1 Protein Associates with RAP30 and Human SPT5 Proteins

Kim, Jae B., Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi, Sharp, Phillip A.

The potent transactivator Tat recognizes the transactivation response RNA element (TAR) of human immunodeficiency virus type 1 and stimulates the processivity of elongation of RNA polymerase (Pol) II...

Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression

Kakugawa, Yoichiro, Wada, Tadashi, Yamaguchi, Kazunori, Yamanami, Hideaki, Ouchi, Kiyoaki, Sato, Ikuro, ...

Human plasma membrane-associated sialidase (Neu3) is unique in specifically hydrolyzing gangliosides, thought to participate in cell differentiation and transmembrane signaling, thereby playing...

Evidence that Negative Elongation Factor Represses Transcription Elongation through Binding to a DRB Sensitivity-Inducing Factor/RNA Polymerase II Complex and RNA

Yamaguchi, Yuki, Inukai, Naoto, Narita, Takashi, Wada, Tadashi, Handa, Hiroshi

Negative elongation factor (NELF) is a human transcription factor complex that cooperates with DRB sensitivity-inducing factor (DSIF)/hSpt4-hSpt5 to repress elongation by RNA polymerase II (RNAPII)....

Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex

Narita, Takashi, Yamaguchi, Yuki, Yano, Keiichi, Sugimoto, Seiji, Chanarat, Sittinan, Wada, Tadashi, ...

The multisubunit transcription elongation factor NELF (for negative elongation factor) acts together with DRB (5,6-dichloro-1-β-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF)/human...

DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs

Wada, Tadashi, Takagi, Toshiyuki, Yamaguchi, Yuki, Ferdous, Anwarul, Imai, Takeshi, Hirose, Susumu, ...

We report the identification of a transcription elongation factor from HeLa cell nuclear extracts that causes pausing of RNA polymerase II (Pol II) in conjunction with the transcription inhibitor...

Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae

Hartzog, Grant A., Wada, Tadashi, Handa, Hiroshi, Winston, Fred

Previous characterization of the Saccharomyces cerevisiae Spt4, Spt5, and Spt6 proteins suggested that these proteins act as transcription factors that modify chromatin structure. In this work, we...

Human Spt6 Stimulates Transcription Elongation by RNA Polymerase II In Vitro

Endoh, Masaki, Zhu, Wenyan, Hasegawa, Jun, Watanabe, Hajime, Kim, Dong-Ki, Aida, Masatoshi, ...

Recent studies have suggested that Spt6 participates in the regulation of transcription by RNA polymerase II (RNAPII). However, its underlying mechanism remains largely unknown. One possibility,...

Bromodomain Factor 1 (Bdf1) Is Phosphorylated by Protein Kinase CK2

Sawa, Chika, Nedea, Eduard, Krogan, Nevan, Wada, Tadashi, Handa, Hiroshi, Greenblatt, Jack, ...

Bromodomain factor 1 (Bdf1) associates with Saccharomyces cerevisiae TFIID and corresponds to the C-terminal half of higher eukaryotic TAF1. It also associates with the SWR-C complex, which is...

Functional interactions of RNA-capping enzyme with factors that positively and negatively regulate promoter escape by RNA polymerase II

Mandal, Subhrangsu S., Chu, Chun, Wada, Tadashi, Handa, Hiroshi, Shatkin, Aaron J., Reinberg, Danny

Capping of the 5′ ends of nascent RNA polymerase II transcripts is the first pre-mRNA processing event in all eukaryotic cells. Capping enzyme (CE) is recruited to transcription complexes soon...

NF-Y Is Essential for the Recruitment of RNA Polymerase II and Inducible Transcription of Several CCAAT Box-Containing Genes

Kabe, Yasuaki, Yamada, Joe, Uga, Hitoshi, Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi

Osteoclast differentiation factor (ODF)/receptor activator of NF-κB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the...

Evidence for mitochondrial localization of a novel human sialidase (NEU4)

Yamaguchi, Kazunori, Hata, Keiko, Koseki, Koichi, Shiozaki, Kazuhiro, Akita, Hirotoshi, Wada, Tadashi, ...

Based on the human cDNA sequence predicted to represent the NEU4 sialidase gene in public databases, a cDNA covering the entire coding sequence was isolated from human brain and expressed in...

Infertility with Defective Spermiogenesis in Mice Lacking AF5q31, the Target of Chromosomal Translocation in Human Infant Leukemia

Urano, Atsushi, Endoh, Masaki, Wada, Tadashi, Morikawa, Yoshihiro, Itoh, Miyuki, Kataoka, Yuki, ...

AF5q31 (also called MCEF) was identified by its involvement in chromosomal translocation with the gene MLL (mixed lineage leukemia), which is associated with infant acute lymphoblastic leukemia....

Transcriptional Pausing Caused by NELF Plays a Dual Role in Regulating Immediate-Early Expression of the junB Gene

Aida, Masatoshi, Chen, Yexi, Nakajima, Koichi, Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi

Human 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) negatively regulate transcription elongation by RNA polymerase II...

Tat-SF1 Protein Associates with RAP30 and Human SPT5 Proteins

Kim, Jae B., Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi, Sharp, Phillip A.

The potent transactivator Tat recognizes the transactivation response RNA element (TAR) of human immunodeficiency virus type 1 and stimulates the processivity of elongation of RNA polymerase (Pol) II...

Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression

Kakugawa, Yoichiro, Wada, Tadashi, Yamaguchi, Kazunori, Yamanami, Hideaki, Ouchi, Kiyoaki, Sato, Ikuro, ...

Human plasma membrane-associated sialidase (Neu3) is unique in specifically hydrolyzing gangliosides, thought to participate in cell differentiation and transmembrane signaling, thereby playing...

Evidence that Negative Elongation Factor Represses Transcription Elongation through Binding to a DRB Sensitivity-Inducing Factor/RNA Polymerase II Complex and RNA

Yamaguchi, Yuki, Inukai, Naoto, Narita, Takashi, Wada, Tadashi, Handa, Hiroshi

Negative elongation factor (NELF) is a human transcription factor complex that cooperates with DRB sensitivity-inducing factor (DSIF)/hSpt4-hSpt5 to repress elongation by RNA polymerase II (RNAPII)....

Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex

Narita, Takashi, Yamaguchi, Yuki, Yano, Keiichi, Sugimoto, Seiji, Chanarat, Sittinan, Wada, Tadashi, ...

The multisubunit transcription elongation factor NELF (for negative elongation factor) acts together with DRB (5,6-dichloro-1-β-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF)/human...

DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs

Wada, Tadashi, Takagi, Toshiyuki, Yamaguchi, Yuki, Ferdous, Anwarul, Imai, Takeshi, Hirose, Susumu, ...

We report the identification of a transcription elongation factor from HeLa cell nuclear extracts that causes pausing of RNA polymerase II (Pol II) in conjunction with the transcription inhibitor...

Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae

Hartzog, Grant A., Wada, Tadashi, Handa, Hiroshi, Winston, Fred

Previous characterization of the Saccharomyces cerevisiae Spt4, Spt5, and Spt6 proteins suggested that these proteins act as transcription factors that modify chromatin structure. In this work, we...

Human Spt6 Stimulates Transcription Elongation by RNA Polymerase II In Vitro

Endoh, Masaki, Zhu, Wenyan, Hasegawa, Jun, Watanabe, Hajime, Kim, Dong-Ki, Aida, Masatoshi, ...

Recent studies have suggested that Spt6 participates in the regulation of transcription by RNA polymerase II (RNAPII). However, its underlying mechanism remains largely unknown. One possibility,...

Bromodomain Factor 1 (Bdf1) Is Phosphorylated by Protein Kinase CK2

Sawa, Chika, Nedea, Eduard, Krogan, Nevan, Wada, Tadashi, Handa, Hiroshi, Greenblatt, Jack, ...

Bromodomain factor 1 (Bdf1) associates with Saccharomyces cerevisiae TFIID and corresponds to the C-terminal half of higher eukaryotic TAF1. It also associates with the SWR-C complex, which is...

Functional interactions of RNA-capping enzyme with factors that positively and negatively regulate promoter escape by RNA polymerase II

Mandal, Subhrangsu S., Chu, Chun, Wada, Tadashi, Handa, Hiroshi, Shatkin, Aaron J., Reinberg, Danny

Capping of the 5′ ends of nascent RNA polymerase II transcripts is the first pre-mRNA processing event in all eukaryotic cells. Capping enzyme (CE) is recruited to transcription complexes soon...

NF-Y Is Essential for the Recruitment of RNA Polymerase II and Inducible Transcription of Several CCAAT Box-Containing Genes

Kabe, Yasuaki, Yamada, Joe, Uga, Hitoshi, Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi

Osteoclast differentiation factor (ODF)/receptor activator of NF-κB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the...

Evidence for mitochondrial localization of a novel human sialidase (NEU4)

Yamaguchi, Kazunori, Hata, Keiko, Koseki, Koichi, Shiozaki, Kazuhiro, Akita, Hirotoshi, Wada, Tadashi, ...

Based on the human cDNA sequence predicted to represent the NEU4 sialidase gene in public databases, a cDNA covering the entire coding sequence was isolated from human brain and expressed in...

Infertility with Defective Spermiogenesis in Mice Lacking AF5q31, the Target of Chromosomal Translocation in Human Infant Leukemia

Urano, Atsushi, Endoh, Masaki, Wada, Tadashi, Morikawa, Yoshihiro, Itoh, Miyuki, Kataoka, Yuki, ...

AF5q31 (also called MCEF) was identified by its involvement in chromosomal translocation with the gene MLL (mixed lineage leukemia), which is associated with infant acute lymphoblastic leukemia....

Transcriptional Pausing Caused by NELF Plays a Dual Role in Regulating Immediate-Early Expression of the junB Gene

Aida, Masatoshi, Chen, Yexi, Nakajima, Koichi, Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi

Human 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) negatively regulate transcription elongation by RNA polymerase II...

Knock-down of 25 kDa subunit of cleavage factor Im in Hela cells alters alternative polyadenylation within 3′-UTRs

Kubo, Tomohiro, Wada, Tadashi, Yamaguchi, Yuki, Shimizu, Akira, Handa, Hiroshi

Alternative polyadenylation leads to mRNAs with variable 3′ ends. Since a 3′-untranslated region (3′-UTR) often contains cis elements that impact stability or localization of mRNA or...

Adeno-Associated Virus Site-Specific Integration Is Regulated by TRP-185▿

Yamamoto, Noriaki, Suzuki, Masato, Kawano, Masa-aki, Inoue, Takamasa, Takahashi, Ryou-u, Tsukamoto, Hiroko, ...

Adeno-associated virus (AAV) integrates site specifically into the AAVS1 locus on human chromosome 19. Although recruitment of the AAV nonstructural protein Rep78/68 to the Rep binding site (RBS) on...

DSIF contributes to transcriptional activation by DNA-binding activators by preventing pausing during transcription elongation

Zhu, Wenyan, Wada, Tadashi, Okabe, Sachiko, Taneda, Takuya, Yamaguchi, Yuki, Handa, Hiroshi

The transcription elongation factor 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB) sensitivity-inducing factor (DSIF) regulates RNA polymerase II (RNAPII) processivity by promoting, in concert...

Drosophila Blimp-1 Is a Transient Transcriptional Repressor That Controls Timing of the Ecdysone-Induced Developmental Pathway▿

Agawa, Yasuo, Sarhan, Moustafa, Kageyama, Yuji, Akagi, Kazutaka, Takai, Masayoshi, Hashiyama, Kazuya, ...

Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-f1 is expressed in a temporally regulated manner following pulses of ecdysteroid...

A new APE1/Ref-1-dependent pathway leading to reduction of NF-κB and AP-1, and activation of their DNA-binding activity

Ando, Kozue, Hirao, Satoshi, Kabe, Yasuaki, Ogura, Yuji, Sato, Iwao, Yamaguchi, Yuki, ...

APE1/Ref-1 is thought to be a multifunctional protein involved in reduction–oxidation (redox) regulation and base excision DNA repair, and is required for early embryonic development in mice....