Scientific Commons: T. J. Hope

Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. (2006)

Zufferey, R, Donello, J E, Trono, D, Hope, T J

The expression of genes delivered by retroviral vectors is often inefficient, a potential obstacle for their widespread use in human gene therapy. Here, we explored the possibility that the...

Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. (2005)

Zufferey, R, Donello, J E, Trono, Didier, Hope, T J

The expression of genes delivered by retroviral vectors is often inefficient, a potential obstacle for their widespread use in human gene therapy. Here, we explored the possibility that the...

Oligomerization and RNA binding domains of the type 1 human immunodeficiency virus Rev protein: a dual function for an arginine-rich binding motif.

Zapp, M L, Hope, T J, Parslow, T G, Green, M R

The Rev protein of human immunodeficiency virus type 1 is a sequence-specific RNA binding protein that is essential for viral replication. Here we present evidence that Rev is a stable oligomer both...

Steroid-receptor fusion of the human immunodeficiency virus type 1 Rev transactivator: mapping cryptic functions of the arginine-rich motif.

Hope, T J, Huang, X J, McDonald, D, Parslow, T G

The human immunodeficiency virus type 1 (HIV-1) transactivator Rev is a nuclear protein that regulates expression of certain HIV-1 transcripts by binding to an RNA target element (the RRE) present in...

The hepatitis B virus post-transcriptional regulatory element contains two conserved RNA stem-loops which are required for function.

Smith, G J, Donello, J E, Lück, R, Steger, G, Hope, T J

Human Hepatitis B Virus (HBV) RNAs contain a cis -acting sequence, the post-transcriptional regulatory element (HPRE), which facilitates the cytoplasmic localization of intronless transcripts. Our...

The hepatitis B virus posttranscriptional regulatory element is composed of two subelements.

Donello, J E, Beeche, A A, Smith, G J, Lucero, G R, Hope, T J

The RNAs of the hepatitis B virus (HBV) contain a cis-acting regulatory element which facilitates the cytoplasmic localization of unspliced transcripts (J. Huang and T. J. Liang, Mol. Cell. Biol....

Characterization of the nuclear export signal of human T-cell lymphotropic virus type 1 Rex reveals that nuclear export is mediated by position-variable hydrophobic interactions.

Kim, F J, Beeche, A A, Hunter, J J, Chin, D J, Hope, T J

We previously determined that amino acids 64 to 120 of human T-cell lymphotropic virus type 1 (HTLV-1) Rex can restore the function of an effector domain mutant of human immunodeficiency virus type 1...

Posttranscriptional effector domains in the Rev proteins of feline immunodeficiency virus and equine infectious anemia virus.

Mancuso, V A, Hope, T J, Zhu, L, Derse, D, Phillips, T, Parslow, T G

By systematically dissecting the Rev proteins of feline immunodeficiency virus (FIV) and equine infectious anemia virus (EIAV), we have identified within each a short peptide that is functionally...

Minimal Rev-response element for type 1 human immunodeficiency virus.

Huang, X J, Hope, T J, Bond, B L, McDonald, D, Grahl, K, Parslow, T G

Rev protein regulates nuclear export of viral mRNAs that contain a 240-base RNA sequence termed the Rev-response element (RRE). We demonstrate that an 88-base truncated RRE encompassing a known Rev...

Posttranscriptional regulation by the human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex proteins through a heterologous RNA binding site.

McDonald, D, Hope, T J, Parslow, T G

The human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex proteins induce cytoplasmic expression of incompletely spliced viral mRNAs by binding to these mRNAs in the...

Mutational analysis of the human immunodeficiency virus type 1 Rev transactivator: essential residues near the amino terminus.

Hope, T J, McDonald, D, Huang, X J, Low, J, Parslow, T G

The expression of certain mRNAs from human immunodeficiency virus type 1 (HIV-1) is controlled by the viral transactivator Rev, a nucleolar protein that binds a cis-acting element in these mRNAs. Rev...

Effector domains of human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex are functionally interchangeable and share an essential peptide motif.

Hope, T J, Bond, B L, McDonald, D, Klein, N P, Parslow, T G

The human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex transactivators are posttranscriptional regulatory proteins that promote retroviral gene expression by...

trans-dominant inhibition of human immunodeficiency virus type 1 Rev occurs through formation of inactive protein complexes.

Hope, T J, Klein, N P, Elder, M E, Parslow, T G

The human immunodeficiency virus type 1 Rev protein controls expression of certain viral RNAs by binding to these RNAs in the nucleus. To investigate how dominant negative Rev mutants inhibit Rev...

Characterization of immunoglobulin enhancer deletions in murine plasmacytomas.

Aguilera, R J, Hope, T J, Sakano, H

We have analyzed enhancer deletions found in murine plasmacytomas by DNA cloning. This analysis revealed that the deletions occurred between the JH region and the switch region, removing the Ig heavy...

A leucine-rich nuclear export signal in the p53 tetramerization domain: regulation of subcellular localization and p53 activity by NES masking.

Stommel, J M, Marchenko, N D, Jimenez, G S, Moll, U M, Hope, T J, Wahl, G M

Appropriate subcellular localization is crucial for regulating p53 function. We show that p53 export is mediated by a highly conserved leucine-rich nuclear export signal (NES) located in its...

Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication.

Jiang, W, McDonald, D, Hope, T J, Hunter, T

The Cdc7-Dbf4 kinase is essential for regulating initiation of DNA replication in Saccharomyces cerevisiae. Previously, we identified a human Cdc7 homolog, HsCdc7. In this study, we report the...

An N-terminal nuclear export signal is required for the nucleocytoplasmic shuttling of IkappaBalpha.

Johnson, C, Van Antwerp, D, Hope, T J

The potent transcriptional activities of Rel/NF-kappaB proteins are regulated in the cytoplasm and nucleus by the inhibitor, IkappaBalpha. The mechanism, by which IkappaBalpha can either sequester...