Scientific Commons: T. G. Parslow

NMR structure of a biologically active peptide containing the RNA-binding domain of human immunodeficiency virus type 1 Tat.

Mujeeb, A, Bishop, K, Peterlin, B M, Turck, C, Parslow, T G, James, T L

The Tat protein of human immunodeficiency virus type 1 enhances transcription by binding to a specific RNA element on nascent viral transcripts. Binding is mediated by a 10-amino acid basic domain...

Oligomerization and RNA binding domains of the type 1 human immunodeficiency virus Rev protein: a dual function for an arginine-rich binding motif.

Zapp, M L, Hope, T J, Parslow, T G, Green, M R

The Rev protein of human immunodeficiency virus type 1 is a sequence-specific RNA binding protein that is essential for viral replication. Here we present evidence that Rev is a stable oligomer both...

Steroid-receptor fusion of the human immunodeficiency virus type 1 Rev transactivator: mapping cryptic functions of the arginine-rich motif.

Hope, T J, Huang, X J, McDonald, D, Parslow, T G

The human immunodeficiency virus type 1 (HIV-1) transactivator Rev is a nuclear protein that regulates expression of certain HIV-1 transcripts by binding to an RNA target element (the RRE) present in...

In vitro selection of RNAs that bind to the human immunodeficiency virus type-1 gag polyprotein.

Lochrie, M A, Waugh, S, Pratt, D G, Clever, J, Parslow, T G, Polisky, B

RNA ligands that bind to the human immunodeficiency virus type-1 (HIV-1) gag polyprotein with 10(-9) M affinity were isolated from a complex pool of RNAs using an in vitro selection method. The...

RNA secondary structure and binding sites for gag gene products in the 5' packaging signal of human immunodeficiency virus type 1.

Clever, J, Sassetti, C, Parslow, T G

The selective encapsidation of retroviral RNA requires sequences in the Gag protein, as well as a cis-acting RNA packaging signal (psi site) near the 5' end of the genomic transcript. Gag protein of...

Requirements for kissing-loop-mediated dimerization of human immunodeficiency virus RNA.

Clever, J L, Wong, M L, Parslow, T G

Sequences from the 5' end of type 1 human immunodeficiency virus RNA dimerize spontaneously in vitro in a reaction thought to mimic the initial step of genomic dimerization in vivo. Dimer initiation...

Mutant human immunodeficiency virus type 1 genomes with defects in RNA dimerization or encapsidation.

Clever, J L, Parslow, T G

Retrovirus particles each contain two copies of the viral genome in the form of a noncovalently linked RNA dimer. Earlier studies have mapped a cis-acting region near the 5' end of the human...

Functional dissection of the human Bc12 protein: sequence requirements for inhibition of apoptosis.

Hunter, J J, Bond, B L, Parslow, T G

Overexpression of the cytoplasmic oncoprotein Bc12 blocks programmed cell death (apoptosis) in many cellular systems. To map the sequences in Bc12 that are necessary for its activity, we created a...

Posttranscriptional effector domains in the Rev proteins of feline immunodeficiency virus and equine infectious anemia virus.

Mancuso, V A, Hope, T J, Zhu, L, Derse, D, Phillips, T, Parslow, T G

By systematically dissecting the Rev proteins of feline immunodeficiency virus (FIV) and equine infectious anemia virus (EIAV), we have identified within each a short peptide that is functionally...

Functional analysis of interactions between Tat and the trans-activation response element of human immunodeficiency virus type 1 in cells.

Luo, Y, Madore, S J, Parslow, T G, Cullen, B R, Peterlin, B M

Transcriptional trans-activation of the human immunodeficiency virus type 1 long terminal repeat requires that the virally encoded Tat effector interacts with its target trans-activation response...

Minimal Rev-response element for type 1 human immunodeficiency virus.

Huang, X J, Hope, T J, Bond, B L, McDonald, D, Grahl, K, Parslow, T G

Rev protein regulates nuclear export of viral mRNAs that contain a 240-base RNA sequence termed the Rev-response element (RRE). We demonstrate that an 88-base truncated RRE encompassing a known Rev...

Posttranscriptional regulation by the human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex proteins through a heterologous RNA binding site.

McDonald, D, Hope, T J, Parslow, T G

The human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex proteins induce cytoplasmic expression of incompletely spliced viral mRNAs by binding to these mRNAs in the...

Mutational analysis of the human immunodeficiency virus type 1 Rev transactivator: essential residues near the amino terminus.

Hope, T J, McDonald, D, Huang, X J, Low, J, Parslow, T G

The expression of certain mRNAs from human immunodeficiency virus type 1 (HIV-1) is controlled by the viral transactivator Rev, a nucleolar protein that binds a cis-acting element in these mRNAs. Rev...

Effector domains of human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex are functionally interchangeable and share an essential peptide motif.

Hope, T J, Bond, B L, McDonald, D, Klein, N P, Parslow, T G

The human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex transactivators are posttranscriptional regulatory proteins that promote retroviral gene expression by...

trans-dominant inhibition of human immunodeficiency virus type 1 Rev occurs through formation of inactive protein complexes.

Hope, T J, Klein, N P, Elder, M E, Parslow, T G

The human immunodeficiency virus type 1 Rev protein controls expression of certain viral RNAs by binding to these RNAs in the nucleus. To investigate how dominant negative Rev mutants inhibit Rev...

Structure of a nuclease-sensitive region inside the immunoglobin kappa gene: evidence for a role in gene regulation.

Parslow, T G, Granner, D K

A discrete chromatin region inside the active immunoglobulin kappa gene is preferentially accessible to cleavage by nucleolytic enzymes. This region comprises 200-250 bp of DNA, and is situated...

Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.

Parslow, T G, Blair, D L, Murphy, W J, Granner, D K

Recent investigations have suggested that tissue-specific regulatory factors are required for immunoglobulin gene transcription. Cells of the mouse lymphocytoid pre-B-cell line 70Z/3 contain a...

Transgenes expressing the Wnt-1 and int-2 proto-oncogenes cooperate during mammary carcinogenesis in doubly transgenic mice.

Kwan, H, Pecenka, V, Tsukamoto, A, Parslow, T G, Guzman, R, Lin, T P, ...

The Wnt-1 and int-2 proto-oncogenes are transcriptionally activated by mouse mammary tumor virus insertion mutations in virus-induced tumors and encode secretory glycoproteins. To determine whether...