Rines, Daniel R, Gomez-Ferreria, Maria, Zhou, Yingyao, DeJesus, Paul, Grob, Seanna, Batalov, Serge, ...
Abstract Background The mitotic spindle is a complex mechanical apparatus required for accurate segregation of sister chromosomes during mitosis. We designed a genetic screen using automated...
Fuqu Yu, Josephine N. Harada, Helen J. Brown, Hongyu Deng, Moon Jung Song, Ting-Ting Wu, ...
The herpesvirus life cycle has two distinct phases: latency and lytic replication. The balance between these two phases is critical for viral pathogenesis. It is believed that cellular signals...
Minimizing the risk of reporting false positives in large-scale RNAi screens (2006)
Echeverri, Christophe J, Beachy, Philip A, Baum, Buzz, Boutros, Michael, Buchholz, Frank, Chanda, Sumit K, ...
Large-scale RNA interference (RNAi)-based analyses, very much as other 'omic' approaches, have inherent rates of false positives and negatives. The variability in the standards of care applied to...
Chao, Sheng-Hao, Walker, John R., Chanda, Sumit K., Gray, Nathanael S., Caldwell, Jeremy S.
Cyclin-dependent kinase inhibitors (CDKIs) have been shown to block human immunodeficiency virus and herpes simplex virus. It is hypothesized that CDKIs block viral replication by inhibiting...
Genome-scale functional profiling of the mammalian AP-1 signaling pathway
Chanda, Sumit K., White, Suhaila, Orth, Anthony P., Reisdorph, Richard, Miraglia, Loren, Thomas, Russell S., ...
Large-scale functional genomics approaches are fundamental to the characterization of mammalian transcriptomes annotated by genome sequencing projects. Although current high-throughput strategies...
Hoxa9 and Meis1 Are Key Targets for MLL-ENL-Mediated Cellular Immortalization
Zeisig, Bernd B., Milne, Tom, García-Cuéllar, María-Paz, Schreiner, Silke, Martin, Mary-Ellen, Fuchs, Uta, ...
MLL fusion proteins are oncogenic transcription factors that are associated with aggressive lymphoid and myeloid leukemias. We constructed an inducible MLL fusion, MLL-ENL-ERtm, that rendered the...
Identification of p53 regulators by genome-wide functional analysis
Huang, Qihong, Raya, Angel, DeJesus, Paul, Chao, Sheng-Hao, Quon, Kim C., Caldwell, Jeremy S., ...
The p53 tumor-suppressor protein is a critical mediator of cellular growth arrest and the induction of apoptosis. To identify proteins involved in the modulation of p53 transcriptional activity, a...
Liu, Jun, Bang, Anne G., Kintner, Chris, Orth, Anthony P., Chanda, Sumit K., Ding, Sheng, ...
The Wnt signaling pathway acts ubiquitously in metazoans to control various aspects of embryonic development. Wnt ligands bind their receptors Frizzled and low-density lipoprotein receptor-related...
Harada, Josephine N., Bower, Kristen E., Orth, Anthony P., Callaway, Scott, Nelson, Christian G., Laris, Casey, ...
Functional profiling technologies using arrayed collections of genome-scale siRNA and cDNA arrayed libraries enable the comprehensive global analysis of gene function. However, the current repertoire...
Chao, Sheng-Hao, Walker, John R., Chanda, Sumit K., Gray, Nathanael S., Caldwell, Jeremy S.
Cyclin-dependent kinase inhibitors (CDKIs) have been shown to block human immunodeficiency virus and herpes simplex virus. It is hypothesized that CDKIs block viral replication by inhibiting...
Genome-scale functional profiling of the mammalian AP-1 signaling pathway
Chanda, Sumit K., White, Suhaila, Orth, Anthony P., Reisdorph, Richard, Miraglia, Loren, Thomas, Russell S., ...
Large-scale functional genomics approaches are fundamental to the characterization of mammalian transcriptomes annotated by genome sequencing projects. Although current high-throughput strategies...
Hoxa9 and Meis1 Are Key Targets for MLL-ENL-Mediated Cellular Immortalization
Zeisig, Bernd B., Milne, Tom, García-Cuéllar, María-Paz, Schreiner, Silke, Martin, Mary-Ellen, Fuchs, Uta, ...
MLL fusion proteins are oncogenic transcription factors that are associated with aggressive lymphoid and myeloid leukemias. We constructed an inducible MLL fusion, MLL-ENL-ERtm, that rendered the...
Identification of p53 regulators by genome-wide functional analysis
Huang, Qihong, Raya, Angel, DeJesus, Paul, Chao, Sheng-Hao, Quon, Kim C., Caldwell, Jeremy S., ...
The p53 tumor-suppressor protein is a critical mediator of cellular growth arrest and the induction of apoptosis. To identify proteins involved in the modulation of p53 transcriptional activity, a...
Liu, Jun, Bang, Anne G., Kintner, Chris, Orth, Anthony P., Chanda, Sumit K., Ding, Sheng, ...
The Wnt signaling pathway acts ubiquitously in metazoans to control various aspects of embryonic development. Wnt ligands bind their receptors Frizzled and low-density lipoprotein receptor-related...
Harada, Josephine N., Bower, Kristen E., Orth, Anthony P., Callaway, Scott, Nelson, Christian G., Laris, Casey, ...
Functional profiling technologies using arrayed collections of genome-scale siRNA and cDNA arrayed libraries enable the comprehensive global analysis of gene function. However, the current repertoire...
Systematic Identification of Cellular Signals Reactivating Kaposi Sarcoma–Associated Herpesvirus
Yu, Fuqu, Harada, Josephine N, Brown, Helen J, Deng, Hongyu, Song, Moon Jung, Wu, Ting-Ting, ...
The herpesvirus life cycle has two distinct phases: latency and lytic replication. The balance between these two phases is critical for viral pathogenesis. It is believed that cellular signals...
Genome-wide functional analysis of human cell-cycle regulators
Mukherji, Mridul, Bell, Russell, Supekova, Lubica, Wang, Yan, Orth, Anthony P., Batalov, Serge, ...
Human cells have evolved complex signaling networks to coordinate the cell cycle. A detailed understanding of the global regulation of this fundamental process requires comprehensive identification...
Agarwal, Sumit, Harada, Josephine, Schreifels, Jeffrey, Lech, Patrycja, Nikolai, Bryan, Yamaguchi, Tomoyuki, ...
By using a genetic screen, we have isolated a mammalian cell line that is resistant to infection by retroviruses that are derived from the murine leukemia virus, human immunodeficiency virus type 1,...
Li, Wei, Bengtson, Mario H., Ulbrich, Axel, Matsuda, Akio, Reddy, Venkateshwar A., Orth, Anthony, ...
Specificity of protein ubiquitylation is conferred by E3 ubiquitin (Ub) ligases. We have annotated ∼617 putative E3s and substrate-recognition subunits of E3 complexes encoded in the human genome....
A role for IκB kinase 2 in bipolar spindle assembly
Irelan, Jeffrey T., Murphy, Thomas J., DeJesus, Paul D., Teo, Hsiangling, Xu, DingYue, Gomez-Ferreria, Maria A., ...
IκB kinase 2 (IKK2 or IKKβ) is a component of the IKK complex that coordinates the cellular response to a diverse set of extracellular stimuli, including cytokines, microbial infection, and stress....
Rines, Daniel R, Gomez-Ferreria, Maria Ana, Zhou, Yingyao, DeJesus, Paul, Grob, Seanna, Batalov, Serge, ...
A loss-of-function screen for siRNAs that arrest human cells in metaphase reveals genes involved in mitotic spindle integrity.