Richard G. F. Visser

Sucrose Metabolism in Plastids1

Gerrits, Nathalie, Hulleman, Stephan H.D., Visser, Richard G.F., Weisbeek, Peter J., ...

The question whether sucrose (Suc) is present inside plastids has been long debated. Low Suc levels were reported to be present inside isolated chloroplasts, but these were argued to be artifacts of...

Discrete Forms of Amylose Are Synthesized by Isoforms of GBSSI in PeaW⃞

Edwards, Anne, Vincken, Jean-Paul, Visser, Richard G. F., Zeeman, Sam, Smith, Alison, ...

Amyloses with distinct molecular masses are found in the starch of pea embryos compared with the starch of pea leaves. In pea embryos, a granule-bound starch synthase protein (GBSSIa) is required for...

A new and versatile method for the successful conversion of AFLP markers into simple single locus markers

Brugmans, Bart, Visser, Richard G. F., Lindhout, Pim, Van Eck, Herman J.

Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required. However, the conversion of...

Expression Analysis of a Family of nsLTP Genes Tissue Specifically Expressed throughout the Plant and during Potato Tuber Life Cycle1

Horvath, Beatrix M., Bachem, Christian W.B., Trindade, Luisa M., Oortwijn, Marian E.P., Visser, Richard G.F.

Non-specific lipid-transfer proteins (nsLTPs) are capable of binding lipid compounds in plant tissues and are coded by the nsLTP genes. Here, we present the analysis of expression of a family of...

Isolation of a Gene Encoding a Copper Chaperone for the Copper/Zinc Superoxide Dismutase and Characterization of Its Promoter in Potato

Trindade, Luisa M., Horvath, Beatrix M., Bergervoet, Marjan J.E., Visser, Richard G.F.

Gene expression during the potato (Solanum tuberosum) tuber lifecycle was monitored by cDNA-amplified fragment-length polymorphism, and several differentially expressed transcript-derived fragments...

Identification of Granule-Bound Starch Synthase in Potato Tubers 1

Vos-Scheperkeuter, Greetje H., De Boer, Wietse, Visser, Richard G. F., Feenstra, Will J., Witholt, Bernard

Starch granules isolated from potato (Solanum tuberosum L.) tubers were extracted with sodium dodecyl sulfate and the extract was analyzed. A major protein with a molecular weight of 60,000 daltons...

Construction of a 10,000-Marker Ultradense Genetic Recombination Map of Potato: Providing a Framework for Accelerated Gene Isolation and a Genomewide Physical Map

Van Os, Hans, Andrzejewski, Sandra, Bakker, Erin, Barrena, Imanol, Bryan, Glenn J., Caromel, Bernard, ...

An ultradense genetic linkage map with >10,000 AFLP loci was constructed from a heterozygous diploid potato population. To our knowledge, this is the densest meiotic recombination map ever...

Sucrose Metabolism in Plastids1

Gerrits, Nathalie, Hulleman, Stephan H.D., Visser, Richard G.F., Weisbeek, Peter J., ...

The question whether sucrose (Suc) is present inside plastids has been long debated. Low Suc levels were reported to be present inside isolated chloroplasts, but these were argued to be artifacts of...

Discrete Forms of Amylose Are Synthesized by Isoforms of GBSSI in PeaW⃞

Edwards, Anne, Vincken, Jean-Paul, Visser, Richard G. F., Zeeman, Sam, Smith, Alison, ...

Amyloses with distinct molecular masses are found in the starch of pea embryos compared with the starch of pea leaves. In pea embryos, a granule-bound starch synthase protein (GBSSIa) is required for...

A new and versatile method for the successful conversion of AFLP markers into simple single locus markers

Brugmans, Bart, Visser, Richard G. F., Lindhout, Pim, Van Eck, Herman J.

Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required. However, the conversion of...

Expression Analysis of a Family of nsLTP Genes Tissue Specifically Expressed throughout the Plant and during Potato Tuber Life Cycle1

Horvath, Beatrix M., Bachem, Christian W.B., Trindade, Luisa M., Oortwijn, Marian E.P., Visser, Richard G.F.

Non-specific lipid-transfer proteins (nsLTPs) are capable of binding lipid compounds in plant tissues and are coded by the nsLTP genes. Here, we present the analysis of expression of a family of...

Isolation of a Gene Encoding a Copper Chaperone for the Copper/Zinc Superoxide Dismutase and Characterization of Its Promoter in Potato

Trindade, Luisa M., Horvath, Beatrix M., Bergervoet, Marjan J.E., Visser, Richard G.F.

Gene expression during the potato (Solanum tuberosum) tuber lifecycle was monitored by cDNA-amplified fragment-length polymorphism, and several differentially expressed transcript-derived fragments...

Identification of Granule-Bound Starch Synthase in Potato Tubers 1

Vos-Scheperkeuter, Greetje H., De Boer, Wietse, Visser, Richard G. F., Feenstra, Will J., Witholt, Bernard

Starch granules isolated from potato (Solanum tuberosum L.) tubers were extracted with sodium dodecyl sulfate and the extract was analyzed. A major protein with a molecular weight of 60,000 daltons...

Construction of a 10,000-Marker Ultradense Genetic Recombination Map of Potato: Providing a Framework for Accelerated Gene Isolation and a Genomewide Physical Map

Van Os, Hans, Andrzejewski, Sandra, Bakker, Erin, Barrena, Imanol, Bryan, Glenn J., Caromel, Bernard, ...

An ultradense genetic linkage map with >10,000 AFLP loci was constructed from a heterozygous diploid potato population. To our knowledge, this is the densest meiotic recombination map ever...

Unravelling enzymatic discoloration in potato through a combined approach of candidate genes, QTL, and expression analysis

Werij, Jeroen S., Kloosterman, Bjorn, Celis-Gamboa, Carolina, America, Twan, Visser, Richard G.F., ...

Enzymatic discoloration (ED) of potato tubers was investigated in an attempt to unravel the underlying genetic factors. Both enzyme and substrate concentration have been reported to influence the...

Expression of alternansucrase in potato plants

Kok-Jacon, Géraldine A., Vincken, Jean-Paul, Wang, Denong, Liu, Shaoyi, Visser, Richard G.F.

Alternan, which consists of alternating α-(1→3)/α-(1→6)-linked glucosyl residues, was produced in potato tubers by expressing a mature alternansucrase (Asr) gene from Leuconostoc mesenteroides...

Genetic Positioning of Centromeres Using Half-Tetrad Analysis in a 4x–2x Cross Population of Potato

Park, Tae-Ho, Kim, Jong-Bo, Hutten, Ronald C. B., Van Eck, Herman J., Jacobsen, Evert, Visser, Richard G. F.

From biological and genetic standpoints, centromeres play an important role in the delivery of the chromosome complement to the daughter cells at cell division. The positions of the centromeres of...

EBP1 regulates organ size through cell growth and proliferation in plants

Horváth, Beatrix M, Magyar, Zoltán, Zhang, Yuexing, Hamburger, Anne W, Bakó, László, Visser, Richard G F, ...

Plant organ size shows remarkable uniformity within species indicating strong endogenous control. We have identified a plant growth regulatory gene, functionally and structurally homologous to human...

Promiscuous, non-catalytic, tandem carbohydrate-binding modules modulate the cell-wall structure and development of transgenic tobacco (Nicotiana tabacum) plants

Obembe, Olawole O., Jacobsen, Evert, Timmers, Jaap, Gilbert, Harry, Blake, Anthony W., Knox, J. Paul, ...

We have compared heterologous expression of two types of carbohydrate binding module (CBM) in tobacco cell walls. These are the promiscuous CBM29 modules (a tandem CBM29-1-2 and its single derivative...

Mapping and characterization of novel parthenocarpy QTLs in tomato

Gorguet, Benoit, Eggink, Pieter Martijn, Ocaña, Juan, Tiwari, Aparna, Schipper, Danny, Finkers, Richard, ...

Parthenocarpy is the development of the fruit in absence of pollination and/or fertilization. In tomato, parthenocarpy is considered as an attractive trait to solve the problems of fruit setting...

Effector Genomics Accelerates Discovery and Functional Profiling of Potato Disease Resistance and Phytophthora Infestans Avirulence Genes

Rietman, Hendrik, Krenek, Pavel, Champouret, Nicolas, Young, Carolyn, Oh, Sang-Keun, ...

Potato is the world's fourth largest food crop yet it continues to endure late blight, a devastating disease caused by the Irish famine pathogen Phytophthora infestans. Breeding broad-spectrum...