Richard Bonnet

New TEM Variant (TEM-92) Produced by Proteus mirabilis and Providencia stuartii Isolates

De Champs, Christophe, Monne, Claire, Bonnet, Richard, Sougakoff, Wladimir, Sirot, Danielle, Chanal, Catherine, ...

The sequences of the blaTEM genes encoding TEM-92 in Proteus mirabilis and Providencia stuartii isolates were determined and were found to be identical. Except for positions 218 (Lys-6) and 512...

Prospective Survey of β-Lactamases Produced by Ceftazidime- Resistant Pseudomonas aeruginosa Isolated in a French Hospital in 2000

De Champs, Christophe, Poirel, Laurent, Bonnet, Richard, Sirot, Danielle, Chanal, Catherine, Sirot, Jacques, ...

In 2000, at the Université d'Auvergne teaching hospital in Clermont-Ferrand, France, 44 (6.2%) strains of Pseudomonas aeruginosa were found to be resistant to ceftazidime. After genotyping, 34...

Inducible AmpC β-Lactamase of a New Member Enterobacteriaceae

Bonnet, Richard, Chanal, Catherine, Ageron, Elisabeth, Sirot, Danielle, De Champs, Christophe, Grimont, Patrick, ...

Extensive biochemical testing and 16S rRNA and rpoB sequence analysis revealed that clinical strain CF01Ent1, initially identified as Buttiauxella agrestis by the use of Api 32 biochemical strips, is...

Infection with an Extended-Spectrum β-Lactamase-Producing Strain of Serratia marcescens following Tongue Reconstruction

Dhawan, Benu, Bonnet, Richard, Shukla, N. K., Mathur, Purva, Das, Bimal K., Kapil, Arti

We report a case of postsurgical wound infection of polymicrobial etiology caused by Serratia marcescens and Pseudomonas aeruginosa following the use of a radial forearm free flap for oncological...

Structure-Based Optimization of a Non-β-lactam Lead Results in Inhibitors That Do Not Up-Regulate β-Lactamase Expression in Cell Culture

Tondi, Donatella, Morandi, Federica, Bonnet, Richard, Costi*, M. Paola, Shoichet*, Brian K.

Bacterial expression of β-lactamases is the most widespread resistance mechanism to β-lactam antibiotics, such as penicillins and cephalosporins. There is a pressing need for novel, non-β-lactam...

Atomic Resolution Structures of CTX-M β-Lactamases: Extended Spectrum Activities from Increased Mobility and Decreased Stability

Chen, Yu, Delmas, Julien, Sirot, Jacques, Shoichet, Brian, Bonnet, Richard

Extended spectrum β-lactamases (ESBLs) confer bacterial resistance to third-generation cephalosporins, such as cefotaxime and ceftazidime, increasing hospital mortality rates. Whereas these...

Structure, Function, and Inhibition along the Reaction Coordinate of CTX-M β-Lactamases

Chen, Yu, Shoichet, Brian, Bonnet, Richard

CTX-M enzymes are an emerging group of extended spectrum β-lactamases (ESBLs) that hydrolyze not only the penicillins but also the first-, second-, and third-generation cephalosporins. Although they...

CMT-Type β-Lactamase TEM-125, an Emerging Problem for Extended-Spectrum β-Lactamase Detection†

Robin, Frédéric, Delmas, Julien, Archambaud, Maryse, Schweitzer, Cédric, Chanal, Catherine, Bonnet, Richard

The clinical strain Escherichia coli TO799 was resistant to penicillin-clavulanate combinations and ceftazidime and was not reproducibly detected as an extended-spectrum β-lactamase (ESBL) according...

New TEM Variant (TEM-92) Produced by Proteus mirabilis and Providencia stuartii Isolates

De Champs, Christophe, Monne, Claire, Bonnet, Richard, Sougakoff, Wladimir, Sirot, Danielle, Chanal, Catherine, ...

The sequences of the blaTEM genes encoding TEM-92 in Proteus mirabilis and Providencia stuartii isolates were determined and were found to be identical. Except for positions 218 (Lys-6) and 512...

Prospective Survey of β-Lactamases Produced by Ceftazidime- Resistant Pseudomonas aeruginosa Isolated in a French Hospital in 2000

De Champs, Christophe, Poirel, Laurent, Bonnet, Richard, Sirot, Danielle, Chanal, Catherine, Sirot, Jacques, ...

In 2000, at the Université d'Auvergne teaching hospital in Clermont-Ferrand, France, 44 (6.2%) strains of Pseudomonas aeruginosa were found to be resistant to ceftazidime. After genotyping, 34...

Inducible AmpC β-Lactamase of a New Member Enterobacteriaceae

Bonnet, Richard, Chanal, Catherine, Ageron, Elisabeth, Sirot, Danielle, De Champs, Christophe, Grimont, Patrick, ...

Extensive biochemical testing and 16S rRNA and rpoB sequence analysis revealed that clinical strain CF01Ent1, initially identified as Buttiauxella agrestis by the use of Api 32 biochemical strips, is...

Infection with an Extended-Spectrum β-Lactamase-Producing Strain of Serratia marcescens following Tongue Reconstruction

Dhawan, Benu, Bonnet, Richard, Shukla, N. K., Mathur, Purva, Das, Bimal K., Kapil, Arti

We report a case of postsurgical wound infection of polymicrobial etiology caused by Serratia marcescens and Pseudomonas aeruginosa following the use of a radial forearm free flap for oncological...

CMT-Type β-Lactamase TEM-125, an Emerging Problem for Extended-Spectrum β-Lactamase Detection†

Robin, Frédéric, Delmas, Julien, Archambaud, Maryse, Schweitzer, Cédric, Chanal, Catherine, Bonnet, Richard

The clinical strain Escherichia coli TO799 was resistant to penicillin-clavulanate combinations and ceftazidime and was not reproducibly detected as an extended-spectrum β-lactamase (ESBL) according...

qnrA in CTX-M-Producing Escherichia coli Isolates from France▿

Lavigne, Jean-Philippe, Marchandin, Hélène, Delmas, Julien, Bouziges, Nicole, Lecaillon, Evelyne, Cavalie, Laurent, ...

By PCR, we screened for qnr genes 112 clinical isolates of extended-spectrum β-lactamase-producing Escherichia coli collected from hospitals in France during 2004. For the first time, 7.7% of...

CTX-M β-Lactamase-Producing Escherichia coli in French Hospitals: Prevalence, Molecular Epidemiology, and Risk Factors▿

Lavigne, Jean-Philippe, Marchandin, Hélène, Delmas, Julien, Moreau, Jérôme, Bouziges, Nicole, Lecaillon, Evelyne, ...

In 2004, 65 CTX-M-producing Escherichia coli isolates were collected from infected patients in four French hospitals. The blaCTX-M-15 genes were predominant. Pulsed-field gel electrophoresis...

Evolution of TEM-Type Enzymes: Biochemical and Genetic Characterization of Two New Complex Mutant TEM Enzymes, TEM-151 and TEM-152, from a Single Patient▿ †

Robin, Frédéric, Delmas, Julien, Schweitzer, Cédric, Tournilhac, Olivier, Lesens, Olivier, Chanal, Catherine, ...

Two clinical isolates of Escherichia coli, CF1179 and CF1295, were isolated from a patient hospitalized in the hematology unit of the University Hospital of Clermont-Ferrand, Clermont-Ferrand,...

Evaluation of a New Chromogenic Medium, chromID VRE, for Detection of Vancomycin-Resistant Enterococci in Stool Samples and Rectal Swabs▿

Delmas, Julien, Robin, Frédéric, Schweitzer, Cédric, Lesens, Olivier, Bonnet, Richard

We compared ChromID VRE medium with Enterococcosel containing vancomycin for the detection of vancomycin-resistant Enterococcus in 1,007 specimens. ChromID VRE in combination with Gram straining...

TEM-158 (CMT-9), a New Member of the CMT-Type Extended-Spectrum β-Lactamases▿

Robin, Frédéric, Delmas, Julien, Brebion, Amélie, Dubois, Damien, Constantin, Jean-Michel, Bonnet, Richard

TEM-158 was found to include the substitutions previously observed for TEM-12 and TEM-35. This enzyme presented hydrolytic activity against ceftazidime and a high level of resistance against...

First Occurrence of an IMP Metallo-β-Lactamase in Aeromonas caviae: IMP-19 in an Isolate from France▿

Neuwirth, Catherine, Siebor, Eliane, Robin, Frederic, Bonnet, Richard

We describe the first IMP metallo-β-lactamase in Aeromonas caviae: IMP-19, which differed from IMP-2 by a single amino acid change (Arg to Ala at position 38). blaIMP-19 was found within a class 1...

Evaluation of the Vitek 2 System with a Variety of Staphylococcus Species▿

Delmas, Julien, Chacornac, Jean Paul, Robin, Frédéric, Giammarinaro, Philippe, Talon, Régine, Bonnet, Richard

The Vitek 2 gram-positive (GP) card was compared with an oligonucleotide array approach for the identification of 190 Staphylococcus strains, including 35 species, isolated from clinical and...

The Lys234Arg Substitution in the Enzyme SHV-72 Is a Determinant for Resistance to Clavulanic Acid Inhibition▿

Mendonça, Nuno, Manageiro, Vera, Robin, Frédéric, Salgado, M. José, Ferreira, Eugénia, Caniça, Manuela, ...

The new β-lactamase SHV-72 was isolated from clinical Klebsiella pneumoniae INSRA1229, which exhibited the unusual association of resistance to the amoxicillin-clavulanic acid combination (MIC, 64...