R. Scott Hawley

The Inhibition of Polo Kinase by Matrimony Maintains G2 Arrest in the Meiotic Cell Cycle (2007)

Youbin Xiang, Satomi Takeo, Laurence Florens, Stacie E. Hughes, Li-Jun Huo, William D. Gilliland, ...

Many meiotic systems in female animals include a lengthy arrest in G2 that separates the end of pachytene from nuclear envelope breakdown (NEB). However, the mechanisms by which a meiotic cell can...

The Multiple Roles of Mps1 in Drosophila Female Meiosis (2007)

William D. Gilliland, Stacie E. Hughes, Jeffrey L. Cotitta, Satomi Takeo, Youbin Xiang, R. Scott Hawley

The Drosophila gene ald encodes the fly ortholog of mps1, a conserved kinetochore-associated protein kinase required for the meiotic and mitotic spindle assembly checkpoints. Using live imaging, we...

The Multiple Roles of Mps1 in Drosophila Female Meiosis (2007)

William Dean Gilliland, Stacie E. Hughes, Jeffrey L. Cotitta, Satomi Takeo, Youbin Xiang, R. Scott Hawley

The Drosophila gene ald encodes the fly ortholog of mps1, a conserved kinetochore-associated protein kinase required for the meiotic and mitotic spindle assembly checkpoints. Loss of this checkpoint...

Drosophila nod protein binds preferentially to the plus ends of microtubules and promotes microtubule polymerization in vitro (2005)

Hawley, R Scott, Gilbert, SP, Matthies, HJG, Gustafson, SM, Sproul, LR, Cui, W

Nod, a nonmotile kinesinlike protein, plays a critical role in segregating achiasmate chromosomes during female meiosis. In addition to localizing to oocyte chromosomes, we show that functional...

All Paired Up with No Place to Go: Pairing, Synapsis, and DSB Formation in a Balancer Heterozygote (2005)

Wei J. Gong, Kim S. McKim, R. Scott Hawley

The multiply inverted X chromosome balancer FM7 strongly suppresses, or eliminates, the occurrence of crossing over when heterozygous with a normal sequence homolog. We have utilized the LacI-GFP:...

All Paired-Up with No Place To Go: Pairing, Synapsis, and DSB Formation in A Balancer Heterozygote (2005)

Wei J. Gong, Kim McKim, R. Scott Hawley

The multiply inverted X chromosome balancer FM7 strongly suppresses, or eliminates, the occurrence of crossing over when heterozygous with a normal sequence homolog. We have utilized the LacI-GFP:...

Juxtaposition of C(2)M and the transverse filament protein C(3)G within the central region of Drosophila synaptonemal complex (2005)

Anderson, Lorinda K., Royer, Suzanne M., Page, Scott L., McKim, Kim S., Lai, Ann, Lilly, Mary A., ...

The synaptonemal complex (SC) is intimately involved in the process of meiotic recombination in most organisms, but its exact role remains enigmatic. One reason for this uncertainty is that the...

Juxtaposition of C(2)M and the transverse filament protein C(3)G within the central region of Drosophila synaptonemal complex (2005)

Anderson, Lorinda K., Royer, Suzanne M., Page, Scott L., McKim, Kim S., Lai, Ann, Lilly, Mary A., ...

The synaptonemal complex (SC) is intimately involved in the process of meiotic recombination in most organisms, but its exact role remains enigmatic. One reason for this uncertainty is that the...

Chromosome choreography: The meiotic ballet (2003)

Page, Scott L., Hawley, R. Scott

The separation of homologous chromosomes during meiosis in eukaryotes is the physical basis of Mendelian inheritance. The core of the meiotic process is a specialized nuclear division (meiosis I) in...

Chromosome choreography : the meiotic ballet (2003)

Page, Scott L., Hawley, R. Scott

The separation of homologous chromosomes during meiosis in eukaryotes is the physical basis of Mendelian inheritance. The core of the meiotic process is a specialized nuclear division (meiosis I) in...

Orphan Kinesin NOD Lacks Motile Properties But Does Possess a Microtubule-stimulated ATPase Activity

Matthies, Heinrich J.G., Baskin, Ronald J., Hawley, R. Scott

NOD is a Drosophila chromosome-associated kinesin-like protein that does not fall into the chromokinesin subfamily. Although NOD lacks residues known to be critical for kinesin function, we show that...

mus304 encodes a novel DNA damage checkpoint protein required during Drosophila development

Brodsky, Michael H., Sekelsky, Jeff J., Tsang, Garson, Hawley, R. Scott, Rubin, Gerald M.

Checkpoints block cell cycle progression in eukaryotic cells exposed to DNA damaging agents. We show that several Drosophila homologs of checkpoint genes, mei-41, grapes, and 14-3-3ε, regulate a DNA...

Juxtaposition of C(2)M and the transverse filament protein C(3)G within the central region of Drosophila synaptonemal complex

Anderson, Lorinda K., Royer, Suzanne M., Page, Scott L., McKim, Kim S., Lai, Ann, Lilly, Mary A., ...

The synaptonemal complex (SC) is intimately involved in the process of meiotic recombination in most organisms, but its exact role remains enigmatic. One reason for this uncertainty is that the...

All Paired Up with No Place to Go: Pairing, Synapsis, and DSB Formation in a Balancer Heterozygote

Gong, Wei J, McKim, Kim S, Hawley, R. Scott

The multiply inverted X chromosome balancer FM7 strongly suppresses, or eliminates, the occurrence of crossing over when heterozygous with a normal sequence homolog. We have utilized the LacI-GFP:...

c(3)G encodes a Drosophila synaptonemal complex protein

Page, Scott L., Hawley, R. Scott

The meiotic mutant c(3)G (crossover suppressor on 3 of Gowen) abolishes both synaptonemal complex (SC) formation and meiotic recombination, whereas mutations in the mei-W68 and mei-P22 genes prevent...

The Effect of mei-41 on Rdna Redundancy in DROSOPHILA MELANOGASTER

Hawley, R. Scott, Tartof, Kenneth D.

The recombination and repair defective mutant, mei-41, exhibits three rather striking effects on the genetic properties and chromosomal stability of rDNA in Drosophila. First, mei-41 inhibits rDNA...

A Two-Stage Model for the Control of rDNA Magnification

Hawley, R. Scott, Tartof, Kenneth D.

Males of the genotype bb/Ybb- have been shown to produce both magnified (bbm+) and, less frequently, reduced (bbrl) X chromosomes. An analysis of the progeny of single magnifying bb/Ybb- males...

Autosomal Modifiers of the Bobbed Phenotype Are a Major Component of the Rdna Magnification Paradox in DROSOPHILA MELANOGASTER

Marcus, Craig H., Zitron, Anne E., Wright, David A., Hawley, R. Scott

rDNA magnification in Drosophila melanogaster is defined experimentally as the ability of bb/Ybb- males to produce exceptional progeny that are wild type with respect to rDNA associated phenotypes....

Chromosomal Sites Necessary for Normal Levels of Meiotic Recombination in DROSOPHILA MELANOGASTER. I. Evidence for and Mapping of the Sites

Hawley, R. Scott

Meiotic exchange was measured in females heterozygous for a normal sequence X chromosome and for each of eleven T(1;4)s and each of sixteen T(1;Y)s. The results indicate that the X chromosome can be...

The Drosophila Meiotic Mutant mei-352 Is an Allele of klp3A and Reveals a Role for a Kinesin-like Protein in Crossover Distribution

Page, Scott L., Hawley, R. Scott

The semisterile meiotic mutant mei-352 alters the distribution of meiotic exchanges without greatly affecting their total frequency. We show that the mei-352 mutation is an allele of the klp3A gene,...

The HhH(2)/NDD Domain of the Drosophila Nod Chromokinesin-like Protein Is Required for Binding to Chromosomes in the Oocyte Nucleus

Cui, Wei, Hawley, R. Scott

Nod is a chromokinesin-like protein that plays a critical role in segregating achiasmate chromosomes during female meiosis. The C-terminal half of the Nod protein contains two putative DNA-binding...

Phenotypic analysis of separation-of-function alleles of MEI-41, Drosophila ATM/ATR.

Laurençon, Anne, Purdy, Amanda, Sekelsky, Jeff, Hawley, R Scott, Su, Tin Tin

ATM/ATR kinases act as signal transducers in eukaryotic DNA damage and replication checkpoints. Mutations in ATM/ATR homologs have pleiotropic effects that range from sterility to increased killing...

A deficiency screen of the major autosomes identifies a gene (matrimony) that is haplo-insufficient for achiasmate segregation in Drosophila oocytes.

Harris, David, Orme, Charisse, Kramer, Joseph, Namba, Luria, Champion, Mia, Palladino, Michael J, ...

In Drosophila oocytes, euchromatic homolog-homolog associations are released at the end of pachytene, while heterochromatic pairings persist until metaphase I. A screen of 123 autosomal deficiencies...

A large-scale screen for mutagen-sensitive loci in Drosophila.

Laurencon, Anne, Orme, Charisse M, Peters, Heather K, Boulton, Christina L, Vladar, Eszter K, Langley, Sasha A, ...

In a screen for new DNA repair mutants, we tested 6275 Drosophila strains bearing homozygous mutagenized autosomes (obtained from C. Zuker) for hypersensitivity to methyl methanesulfonate (MMS) and...

The Mechanism of Secondary Nondisjunction in Drosophila melanogaster Females

Xiang, Youbin, Hawley, R. Scott

Bridges (1916) observed that X chromosome nondisjunction was much more frequent in XXY females than it was in genetically normal XX females. In addition, virtually all cases of X nondisjunction in...

Sometimes the Result Is Not the Answer: The Truths and the Lies That Come From Using the Complementation Test

Hawley, R. Scott, Gilliland, William D.

It is standard genetic practice to determine whether or not two independently obtained mutants define the same or different genes by performing the complementation test. While the complementation...

Orphan Kinesin NOD Lacks Motile Properties But Does Possess a Microtubule-stimulated ATPase Activity

Matthies, Heinrich J.G., Baskin, Ronald J., Hawley, R. Scott

NOD is a Drosophila chromosome-associated kinesin-like protein that does not fall into the chromokinesin subfamily. Although NOD lacks residues known to be critical for kinesin function, we show that...

c(3)G encodes a Drosophila synaptonemal complex protein

Page, Scott L., Hawley, R. Scott

The meiotic mutant c(3)G (crossover suppressor on 3 of Gowen) abolishes both synaptonemal complex (SC) formation and meiotic recombination, whereas mutations in the mei-W68 and mei-P22 genes prevent...

mus304 encodes a novel DNA damage checkpoint protein required during Drosophila development

Brodsky, Michael H., Sekelsky, Jeff J., Tsang, Garson, Hawley, R. Scott, Rubin, Gerald M.

Checkpoints block cell cycle progression in eukaryotic cells exposed to DNA damaging agents. We show that several Drosophila homologs of checkpoint genes, mei-41, grapes, and 14-3-3ε, regulate a DNA...

Juxtaposition of C(2)M and the transverse filament protein C(3)G within the central region of Drosophila synaptonemal complex

Anderson, Lorinda K., Royer, Suzanne M., Page, Scott L., McKim, Kim S., Lai, Ann, Lilly, Mary A., ...

The synaptonemal complex (SC) is intimately involved in the process of meiotic recombination in most organisms, but its exact role remains enigmatic. One reason for this uncertainty is that the...

The Effect of mei-41 on Rdna Redundancy in DROSOPHILA MELANOGASTER

Hawley, R. Scott, Tartof, Kenneth D.

The recombination and repair defective mutant, mei-41, exhibits three rather striking effects on the genetic properties and chromosomal stability of rDNA in Drosophila. First, mei-41 inhibits rDNA...

A Two-Stage Model for the Control of rDNA Magnification

Hawley, R. Scott, Tartof, Kenneth D.

Males of the genotype bb/Ybb- have been shown to produce both magnified (bbm+) and, less frequently, reduced (bbrl) X chromosomes. An analysis of the progeny of single magnifying bb/Ybb- males...

Autosomal Modifiers of the Bobbed Phenotype Are a Major Component of the Rdna Magnification Paradox in DROSOPHILA MELANOGASTER

Marcus, Craig H., Zitron, Anne E., Wright, David A., Hawley, R. Scott

rDNA magnification in Drosophila melanogaster is defined experimentally as the ability of bb/Ybb- males to produce exceptional progeny that are wild type with respect to rDNA associated phenotypes....

Chromosomal Sites Necessary for Normal Levels of Meiotic Recombination in DROSOPHILA MELANOGASTER. I. Evidence for and Mapping of the Sites

Hawley, R. Scott

Meiotic exchange was measured in females heterozygous for a normal sequence X chromosome and for each of eleven T(1;4)s and each of sixteen T(1;Y)s. The results indicate that the X chromosome can be...

All Paired Up with No Place to Go: Pairing, Synapsis, and DSB Formation in a Balancer Heterozygote

Gong, Wei J, McKim, Kim S, Hawley, R. Scott

The multiply inverted X chromosome balancer FM7 strongly suppresses, or eliminates, the occurrence of crossing over when heterozygous with a normal sequence homolog. We have utilized the LacI-GFP:...

The Drosophila Meiotic Mutant mei-352 Is an Allele of klp3A and Reveals a Role for a Kinesin-like Protein in Crossover Distribution

Page, Scott L., Hawley, R. Scott

The semisterile meiotic mutant mei-352 alters the distribution of meiotic exchanges without greatly affecting their total frequency. We show that the mei-352 mutation is an allele of the klp3A gene,...

The HhH(2)/NDD Domain of the Drosophila Nod Chromokinesin-like Protein Is Required for Binding to Chromosomes in the Oocyte Nucleus

Cui, Wei, Hawley, R. Scott

Nod is a chromokinesin-like protein that plays a critical role in segregating achiasmate chromosomes during female meiosis. The C-terminal half of the Nod protein contains two putative DNA-binding...

Phenotypic analysis of separation-of-function alleles of MEI-41, Drosophila ATM/ATR.

Laurençon, Anne, Purdy, Amanda, Sekelsky, Jeff, Hawley, R Scott, Su, Tin Tin

ATM/ATR kinases act as signal transducers in eukaryotic DNA damage and replication checkpoints. Mutations in ATM/ATR homologs have pleiotropic effects that range from sterility to increased killing...

A deficiency screen of the major autosomes identifies a gene (matrimony) that is haplo-insufficient for achiasmate segregation in Drosophila oocytes.

Harris, David, Orme, Charisse, Kramer, Joseph, Namba, Luria, Champion, Mia, Palladino, Michael J, ...

In Drosophila oocytes, euchromatic homolog-homolog associations are released at the end of pachytene, while heterochromatic pairings persist until metaphase I. A screen of 123 autosomal deficiencies...

A large-scale screen for mutagen-sensitive loci in Drosophila.

Laurencon, Anne, Orme, Charisse M, Peters, Heather K, Boulton, Christina L, Vladar, Eszter K, Langley, Sasha A, ...

In a screen for new DNA repair mutants, we tested 6275 Drosophila strains bearing homozygous mutagenized autosomes (obtained from C. Zuker) for hypersensitivity to methyl methanesulfonate (MMS) and...

The Mechanism of Secondary Nondisjunction in Drosophila melanogaster Females

Xiang, Youbin, Hawley, R. Scott

Bridges (1916) observed that X chromosome nondisjunction was much more frequent in XXY females than it was in genetically normal XX females. In addition, virtually all cases of X nondisjunction in...

Sometimes the Result Is Not the Answer: The Truths and the Lies That Come From Using the Complementation Test

Hawley, R. Scott, Gilliland, William D.

It is standard genetic practice to determine whether or not two independently obtained mutants define the same or different genes by performing the complementation test. While the complementation...

The Multiple Roles of Mps1 in Drosophila Female Meiosis

Gilliland, William D, Hughes, Stacie E, Cotitta, Jeffrey L, Takeo, Satomi, Xiang, Youbin, Hawley, R. Scott

The Drosophila gene ald encodes the fly ortholog of mps1, a conserved kinetochore-associated protein kinase required for the meiotic and mitotic spindle assembly checkpoints. Using live imaging, we...

A Genetic Analysis of the Drosophila mcm5 Gene Defines a Domain Specifically Required for Meiotic Recombination

Lake, Cathleen M., Teeter, Kathy, Page, Scott L., Nielsen, Rachel, Hawley, R. Scott

Members of the minichromosome maintenance (MCM) family have pivotal roles in many biological processes. Although originally studied for their role in DNA replication, it is becoming increasingly...

The Inhibition of Polo Kinase by Matrimony Maintains G2 Arrest in the Meiotic Cell Cycle

Xiang, Youbin, Takeo, Satomi, Florens, Laurence, Hughes, Stacie E, Huo, Li-Jun, Gilliland, William D, ...

Many meiotic systems in female animals include a lengthy arrest in G2 that separates the end of pachytene from nuclear envelope breakdown (NEB). However, the mechanisms by which a meiotic cell can...

Mutations in the α-Tubulin 67C Gene Specifically Impair Achiasmate Segregation in Drosophila melanogaster

Matthies, Heinrich J.G., Messina, Lisa G., Namba, Ruria, Greer, Kimberly J., Walker, M.Y., Hawley, R. Scott

Drosophila melanogaster oocytes heterozygous for mutations in the α-tubulin 67C gene (αtub67C) display defects in centromere positioning during prometaphase of meiosis I. The centromeres do not...