Pietro Ghezzi

Redox regulation of chemokine receptor expression

Saccani, Alessandra, Saccani, Simona, Orlando, Simone, Sironi, Marina, Bernasconi, Sergio, Ghezzi, Pietro, ...

Cytokines and reactive oxygen intermediates (ROI) are frequent companions at sites of acute inflammation. We have shown previously that in human monocytes, bacterial lipopolysaccharide, IL-1, and...

Hyperresponsive febrile reactions to interleukin (IL) 1α and IL-1β, and altered brain cytokine mRNA and serum cytokine levels, in IL-1β-deficient mice

Alheim, Katarina, Chai, Zhen, Fantuzzi, Giamila, Hasanvan, Homa, Malinowsky, David, Di Santo, Elena, ...

IL-1β is an endogenous pyrogen that is induced during systemic lipopolysaccharide (LPS)- or IL-1-induced fever. We have examined the fever and cytokine responses following i.p. injection of IL-1...

Erythropoietin crosses the blood–brain barrier to protect against experimental brain injury

Brines, Michael L., Ghezzi, Pietro, Keenan, Sonja, Agnello, Davide, De Lanerolle, Nihal C., Cerami, Carla, ...

Erythropoietin (EPO), recognized for its central role in erythropoiesis, also mediates neuroprotection when the recombinant form (r-Hu-EPO) is directly injected into ischemic rodent brain. We...

Chronic elevation of plasma thioredoxin: Inhibition of chemotaxis and curtailment of life expectancy in AIDS

Nakamura, Hajime, De Rosa, Stephen C., Yodoi, Junji, Holmgren, Arne, Ghezzi, Pietro, Herzenberg, Leonard A., ...

Thioredoxin (Trx) is an intracellular redox protein with extracellular cytokine-like and chemokine-like activities. We show here that, although plasma Trx levels are unrelated to survival of...

Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress

Sirén, Anna-Leena, Fratelli, Maddalena, Brines, Michael, Goemans, Christoph, Casagrande, Simona, Lewczuk, Piotr, ...

Erythropoietin (EPO) promotes neuronal survival after hypoxia and other metabolic insults by largely unknown mechanisms. Apoptosis and necrosis have been proposed as mechanisms of cellular demise,...

Identification by redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes

Fratelli, Maddalena, Demol, Hans, Puype, Magda, Casagrande, Simona, Eberini, Ivano, Salmona, Mario, ...

Formation of mixed disulfides between glutathione and the cysteines of some proteins (glutathionylation) has been suggested as a mechanism through which protein functions can be regulated by the...

Glutathionylation of human thioredoxin: A possible crosstalk between the glutathione and thioredoxin systems

Casagrande, Simona, Bonetto, Valentina, Fratelli, Maddalena, Gianazza, Elisabetta, Eberini, Ivano, Massignan, Tania, ...

To identify proteins undergoing glutathionylation (formation of protein-glutathione mixed disulfides) in human T cell blasts, we radiolabeled the glutathione pool with 35S, exposed cells to the...

Recombinant human erythropoietin protects the myocardium from ischemia-reperfusion injury and promotes beneficial remodeling

Calvillo, Laura, Latini, Roberto, Kajstura, Jan, Leri, Annarosa, Anversa, Piero, Ghezzi, Pietro, ...

Erythropoietin (EPO), originally identified for its critical hormonal role in promoting erythrocyte survival and differentiation, is a member of the large and diverse cytokine superfamily. Recent...

Asialoerythropoietin is a nonerythropoietic cytokine with broad neuroprotective activity in vivo

Erbayraktar, Serhat, Grasso, Giovanni, Sfacteria, Alessandra, Xie, Qiao-wen, Coleman, Thomas, Kreilgaard, Mads, ...

Erythropoietin (EPO) is a tissue-protective cytokine preventing vascular spasm, apoptosis, and inflammatory responses. Although best known for its role in hematopoietic lineages, EPO also affects...

Redox regulation of surface protein thiols: Identification of integrin α-4 as a molecular target by using redox proteomics

Laragione, Teresa, Bonetto, Valentina, Casoni, Filippo, Massignan, Tania, Bianchi, Giancarlo, Gianazza, Elisabetta, ...

Thiols affect a variety of cell functions, an effect known as redox regulation. We show here that treatment (1–2 h) of cells with 0.1–5 mM N-acetyl-l-cysteine (NAC) increases surface protein...

Erythropoietin both protects from and reverses experimental diabetic neuropathy

Bianchi, Roberto, Buyukakilli, Belgin, Brines, Michael, Savino, Costanza, Cavaletti, Guido, Oggioni, Norberto, ...

Erythropoietin (EPO) possesses generalized neuroprotective and neurotrophic actions. We tested the efficacy of recombinant human EPO (rhEPO) in preventing and reversing nerve dysfunction in...

Gene expression profiling reveals a signaling role of glutathione in redox regulation

Fratelli, Maddalena, Goodwin, Leslie O., Ørom, Ulf Andersson, Lombardi, Sarah, Tonelli, Rossella, Mengozzi, Manuela, ...

Proteins can form reversible mixed disulfides with glutathione (GSH). It has been hypothesized that protein glutathionylation may represent a mechanism of redox regulation, in a fashion similar to...

Noncompetitive allosteric inhibitors of the inflammatory chemokine receptors CXCR1 and CXCR2: Prevention of reperfusion injury

Bertini, Riccardo, Allegretti, Marcello, Bizzarri, Cinzia, Moriconi, Alessio, Locati, Massimo, Zampella, Giuseppe, ...

The chemokine CXC ligand 8 (CXCL8)/IL-8 and related agonists recruit and activate polymorphonuclear cells by binding the CXC chemokine receptor 1 (CXCR1) and CXCR2. Here we characterize the unique...

Erythropoietin mediates tissue protection through an erythropoietin and common β-subunit heteroreceptor

Brines, Michael, Grasso, Giovanni, Fiordaliso, Fabio, Sfacteria, Alessandra, Ghezzi, Pietro, Fratelli, Maddalena, ...

The cytokine erythropoietin (Epo) is tissue-protective in preclinical models of ischemic, traumatic, toxic, and inflammatory injuries. We have recently characterized Epo derivatives that do not bind...

A nonerythropoietic derivative of erythropoietin protects the myocardium from ischemia–reperfusion injury

Fiordaliso, Fabio, Chimenti, Stefano, Staszewsky, Lidia, Bai, Antonio, Carlo, Eleonora, Cuccovillo, Ivan, ...

The cytokine erythropoietin (EPO) protects the heart from ischemic injury, in part by preventing apoptosis. However, EPO administration can also raise the hemoglobin concentration, which, by...

Cytoprotective doses of erythropoietin or carbamylated erythropoietin have markedly different procoagulant and vasoactive activities

Coleman, Thomas R., Westenfelder, Christof, Tögel, Florian E., Yang, Ying, Hu, Zhuma, Swenson, LeAnne, ...

Recombinant human erythropoietin (rhEPO) is receiving increasing attention as a potential therapy for prevention of injury and restoration of function in nonhematopoietic tissues. However, the...

Nonhematopoietic Erythropoietin Derivatives Prevent Motoneuron Degeneration In Vitro and In Vivo

Mennini, Tiziana, De Paola, Massimiliano, Bigini, Paolo, Mastrotto, Cristina, Fumagalli, Elena, Barbera, Sara, ...

Chronic treatment with asialo erythropoietin (ASIALO-EPO) or carbamylated erythropoietin (CEPO) improved motor behavior and reduced motoneuron loss and astrocyte and microglia activation in the...

Redox regulation of chemokine receptor expression

Saccani, Alessandra, Saccani, Simona, Orlando, Simone, Sironi, Marina, Bernasconi, Sergio, Ghezzi, Pietro, ...

Cytokines and reactive oxygen intermediates (ROI) are frequent companions at sites of acute inflammation. We have shown previously that in human monocytes, bacterial lipopolysaccharide, IL-1, and...

Hyperresponsive febrile reactions to interleukin (IL) 1α and IL-1β, and altered brain cytokine mRNA and serum cytokine levels, in IL-1β-deficient mice

Alheim, Katarina, Chai, Zhen, Fantuzzi, Giamila, Hasanvan, Homa, Malinowsky, David, Di Santo, Elena, ...

IL-1β is an endogenous pyrogen that is induced during systemic lipopolysaccharide (LPS)- or IL-1-induced fever. We have examined the fever and cytokine responses following i.p. injection of IL-1...

Erythropoietin crosses the blood–brain barrier to protect against experimental brain injury

Brines, Michael L., Ghezzi, Pietro, Keenan, Sonja, Agnello, Davide, De Lanerolle, Nihal C., Cerami, Carla, ...

Erythropoietin (EPO), recognized for its central role in erythropoiesis, also mediates neuroprotection when the recombinant form (r-Hu-EPO) is directly injected into ischemic rodent brain. We...

Chronic elevation of plasma thioredoxin: Inhibition of chemotaxis and curtailment of life expectancy in AIDS

Nakamura, Hajime, De Rosa, Stephen C., Yodoi, Junji, Holmgren, Arne, Ghezzi, Pietro, Herzenberg, Leonard A., ...

Thioredoxin (Trx) is an intracellular redox protein with extracellular cytokine-like and chemokine-like activities. We show here that, although plasma Trx levels are unrelated to survival of...

Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress

Sirén, Anna-Leena, Fratelli, Maddalena, Brines, Michael, Goemans, Christoph, Casagrande, Simona, Lewczuk, Piotr, ...

Erythropoietin (EPO) promotes neuronal survival after hypoxia and other metabolic insults by largely unknown mechanisms. Apoptosis and necrosis have been proposed as mechanisms of cellular demise,...

Identification by redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes

Fratelli, Maddalena, Demol, Hans, Puype, Magda, Casagrande, Simona, Eberini, Ivano, Salmona, Mario, ...

Formation of mixed disulfides between glutathione and the cysteines of some proteins (glutathionylation) has been suggested as a mechanism through which protein functions can be regulated by the...

Glutathionylation of human thioredoxin: A possible crosstalk between the glutathione and thioredoxin systems

Casagrande, Simona, Bonetto, Valentina, Fratelli, Maddalena, Gianazza, Elisabetta, Eberini, Ivano, Massignan, Tania, ...

To identify proteins undergoing glutathionylation (formation of protein-glutathione mixed disulfides) in human T cell blasts, we radiolabeled the glutathione pool with 35S, exposed cells to the...

Recombinant human erythropoietin protects the myocardium from ischemia-reperfusion injury and promotes beneficial remodeling

Calvillo, Laura, Latini, Roberto, Kajstura, Jan, Leri, Annarosa, Anversa, Piero, Ghezzi, Pietro, ...

Erythropoietin (EPO), originally identified for its critical hormonal role in promoting erythrocyte survival and differentiation, is a member of the large and diverse cytokine superfamily. Recent...

Asialoerythropoietin is a nonerythropoietic cytokine with broad neuroprotective activity in vivo

Erbayraktar, Serhat, Grasso, Giovanni, Sfacteria, Alessandra, Xie, Qiao-wen, Coleman, Thomas, Kreilgaard, Mads, ...

Erythropoietin (EPO) is a tissue-protective cytokine preventing vascular spasm, apoptosis, and inflammatory responses. Although best known for its role in hematopoietic lineages, EPO also affects...

Redox regulation of surface protein thiols: Identification of integrin α-4 as a molecular target by using redox proteomics

Laragione, Teresa, Bonetto, Valentina, Casoni, Filippo, Massignan, Tania, Bianchi, Giancarlo, Gianazza, Elisabetta, ...

Thiols affect a variety of cell functions, an effect known as redox regulation. We show here that treatment (1–2 h) of cells with 0.1–5 mM N-acetyl-l-cysteine (NAC) increases surface protein...

Erythropoietin both protects from and reverses experimental diabetic neuropathy

Bianchi, Roberto, Buyukakilli, Belgin, Brines, Michael, Savino, Costanza, Cavaletti, Guido, Oggioni, Norberto, ...

Erythropoietin (EPO) possesses generalized neuroprotective and neurotrophic actions. We tested the efficacy of recombinant human EPO (rhEPO) in preventing and reversing nerve dysfunction in...

Noncompetitive allosteric inhibitors of the inflammatory chemokine receptors CXCR1 and CXCR2: Prevention of reperfusion injury

Bertini, Riccardo, Allegretti, Marcello, Bizzarri, Cinzia, Moriconi, Alessio, Locati, Massimo, Zampella, Giuseppe, ...

The chemokine CXC ligand 8 (CXCL8)/IL-8 and related agonists recruit and activate polymorphonuclear cells by binding the CXC chemokine receptor 1 (CXCR1) and CXCR2. Here we characterize the unique...

Erythropoietin mediates tissue protection through an erythropoietin and common β-subunit heteroreceptor

Brines, Michael, Grasso, Giovanni, Fiordaliso, Fabio, Sfacteria, Alessandra, Ghezzi, Pietro, Fratelli, Maddalena, ...

The cytokine erythropoietin (Epo) is tissue-protective in preclinical models of ischemic, traumatic, toxic, and inflammatory injuries. We have recently characterized Epo derivatives that do not bind...

A nonerythropoietic derivative of erythropoietin protects the myocardium from ischemia–reperfusion injury

Fiordaliso, Fabio, Chimenti, Stefano, Staszewsky, Lidia, Bai, Antonio, Carlo, Eleonora, Cuccovillo, Ivan, ...

The cytokine erythropoietin (EPO) protects the heart from ischemic injury, in part by preventing apoptosis. However, EPO administration can also raise the hemoglobin concentration, which, by...

Gene expression profiling reveals a signaling role of glutathione in redox regulation

Fratelli, Maddalena, Goodwin, Leslie O., Ørom, Ulf Andersson, Lombardi, Sarah, Tonelli, Rossella, Mengozzi, Manuela, ...

Proteins can form reversible mixed disulfides with glutathione (GSH). It has been hypothesized that protein glutathionylation may represent a mechanism of redox regulation, in a fashion similar to...

Cytoprotective doses of erythropoietin or carbamylated erythropoietin have markedly different procoagulant and vasoactive activities

Coleman, Thomas R., Westenfelder, Christof, Tögel, Florian E., Yang, Ying, Hu, Zhuma, Swenson, LeAnne, ...

Recombinant human erythropoietin (rhEPO) is receiving increasing attention as a potential therapy for prevention of injury and restoration of function in nonhematopoietic tissues. However, the...

Nonhematopoietic Erythropoietin Derivatives Prevent Motoneuron Degeneration In Vitro and In Vivo

Mennini, Tiziana, De Paola, Massimiliano, Bigini, Paolo, Mastrotto, Cristina, Fumagalli, Elena, Barbera, Sara, ...

Chronic treatment with asialo erythropoietin (ASIALO-EPO) or carbamylated erythropoietin (CEPO) improved motor behavior and reduced motoneuron loss and astrocyte and microglia activation in the...

The Interleukin-8 (IL-8/CXCL8) Receptor Inhibitor Reparixin Improves Neurological Deficits and Reduces Long-term Inflammation in Permanent and Transient Cerebral Ischemia in Rats

Villa, Pia, Triulzi, Sara, Cavalieri, Barbara, Di Bitondo, Rosa, Bertini, Riccardo, Barbera, Sara, ...

Leukocyte infiltration is viewed as a pharmacological target in cerebral ischemia. We previously reported that reparixin, a CXCL8 receptor blocker that inhibits neutrophil infiltration, and related...

Erythropoietin Selectively Attenuates Cytokine Production and Inflammation in Cerebral Ischemia by Targeting Neuronal Apoptosis

Villa, Pia, Bigini, Paolo, Mennini, Tiziana, Agnello, Davide, Laragione, Teresa, Cagnotto, Alfredo, ...

Ischemic brain injury resulting from stroke arises from primary neuronal losses and by inflammatory responses. Previous studies suggest that erythropoietin (EPO) attenuates both processes. Although...

Thioredoxin, a Redox Enzyme Released in Infection and Inflammation, Is a Unique Chemoattractant for Neutrophils, Monocytes, and T Cells

Bertini, Riccardo, Zack Howard, O.M., Dong, Hui-Fang, Oppenheim, Joost J., Bizzarri, Cinzia, Sergi, Rita, ...

Thioredoxin (Trx) is a ubiquitous intracellular protein disulfide oxidoreductase with a CXXC active site that can be released by various cell types upon activation. We show here that Trx is...

Glucocorticoids as cytokine inhibitors: role in neuroendocrine control and therapy of inflammatory diseases

Fantuzzi, Giamila, Ghezzi, Pietro

Glucocorticoids are potent inhibitors of inflammation and endotoxic shock. This probably occurs through an inhibition of the synthesis of pro-inflammatory cytokines as well as of many of their toxic...

TNF receptor I sensitizes neurons to erythropoietin- and VEGF-mediated neuroprotection after ischemic and excitotoxic injury

Taoufik, Era, Petit, Edwige, Divoux, Didier, Tseveleki, Vivian, Mengozzi, Manuela, Roberts, Michael L., ...

CNS neurons use robust cytoprotective mechanisms to ensure survival and functioning under conditions of injury. These involve pathways induced by endogenous neuroprotective cytokines such as...

Endogenous Erythropoietin as Part of the Cytokine Network in the Pathogenesis of Experimental Autoimmune Encephalomyelitis

Mengozzi, Manuela, Cervellini, Ilaria, Bigini, Paolo, Martone, Sara, Biondi, Antonella, Pedotti, Rosetta, ...

Erythropoietin (EPO) is of great interest as a therapy for many of the central nervous system (CNS) diseases and its administration is protective in experimental autoimmune encephalomyelitis (EAE),...