Partners of Rpb8p, a Small Subunit Shared by Yeast RNA Polymerases I, II, and III
Briand, Jean-François, Navarro, Francisco, Rematier, Peggy, Boschiero, Claire, Labarre, Sylvie, Werner, Michel, ...
Rpb8p, a subunit common to the three yeast RNA polymerases, is conserved among eukaryotes and absent from noneukaryotes. Defective mutants were found at an invariant GGLLM motif and at two other...
Cross Talk between tRNA and rRNA Synthesis in Saccharomyces cerevisiae
Briand, Jean-François, Navarro, Francisco, Gadal, Olivier, Thuriaux, Pierre
Temperature-sensitive RNA polymerase III (rpc160-112 and rpc160-270) mutants were analyzed for the synthesis of tRNAs and rRNAs in vivo, using a double-isotopic-labeling technique in which cells are...
τ91, an Essential Subunit of Yeast Transcription Factor IIIC, Cooperates with τ138 in DNA Binding
Arrebola, Rosalía, Manaud, Nathalie, Rozenfeld, Sophie, Marsolier, Marie-Claude, Lefebvre, Olivier, Carles, Christophe, ...
Transcription factor IIIC (TFIIIC) (or τ) is a large multisubunit and multifunctional factor required for transcription of all class III genes in Saccharomyces cerevisiae. It is responsible for...
Hmo1, an HMG-box protein, belongs to the yeast ribosomal DNA transcription system
Gadal, Olivier, Labarre, Sylvie, Boschiero, Claire, Thuriaux, Pierre
Hmo1 is one of seven HMG-box proteins of Saccharo myces cerevisiae. Null mutants have a limited effect on growth. Hmo1 overexpression suppresses rpa49-Δ mutants lacking Rpa49, a non-essential but...
An Rpb4/Rpb7-Like Complex in Yeast RNA Polymerase III Contains the Orthologue of Mammalian CGRP-RCP
Siaut, Magali, Zaros, Cécile, Levivier, Emilie, Ferri, Maria-Laura, Court, Magali, Werner, Michel, ...
The essential C17 subunit of yeast RNA polymerase (Pol) III interacts with Brf1, a component of TFIIIB, suggesting a role for C17 in the initiation step of transcription. The protein sequence of C17...
Members of the SAGA and Mediator complexes are partners of the transcription elongation factor TFIIS
Wery, Maxime, Shematorova, Elena, Van Driessche, Benoît, Vandenhaute, Jean, Thuriaux, Pierre, Van Mullem, Vincent
TFIIS, an elongation factor encoded by DST1 in Saccharomyces cerevisiae, stimulates transcript cleavage in arrested RNA polymerase II. Two components of the RNA polymerase II machinery, Med13 (Srb9)...
A genetic look at the active site of RNA polymerase III
Rozenfeld, Sophie, Thuriaux, Pierre
rpc160-112, a mutant of the RNA polymerase III active site, is corrected in vivo by six second-site mutants obtained by random mutagenesis. These mutants introduce single-site amino acid replacements...
Ancient origin, functional conservation and fast evolution of DNA-dependent RNA polymerase III
Proshkina, Galina M., Shematorova, Elena K., Proshkin, Sergey A., Zaros, Cécile, Thuriaux, Pierre, Shpakovski, George V.
RNA polymerase III contains seventeen subunits in yeasts (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and in human cells. Twelve of them are akin to the core RNA polymerase I or II. The...
Kohli, Jürg, Hottinger, Herbert, Munz, Peter, Strauss, Andre, Thuriaux, Pierre
The genetic maps of the fission yeast Schizosaccharomyces pombe were extended through the use of haploidization (spontaneous or induced by m-fluorophenylalanine), as well as by tetrad, random spore...
Regulatory Genes Controlling Mitosis in the Fission Yeast SCHIZOSACCHAROMYCES POMBE
Fifty-two wee mutants that undergo mitosis and cell division at a reduced size compared with wild type have been genetically analyzed. The mutants define two genes, wee1 and cdc2, which control the...
Kalogeropoulos, Angelos, Thuriaux, Pierre
A hybrid DNA (hDNA) model of recombination has been algebraically formulated, which allows the prediction of frequencies of postmeiotic segregation and conversion of a given allele and their...
Partners of Rpb8p, a Small Subunit Shared by Yeast RNA Polymerases I, II, and III
Briand, Jean-François, Navarro, Francisco, Rematier, Peggy, Boschiero, Claire, Labarre, Sylvie, Werner, Michel, ...
Rpb8p, a subunit common to the three yeast RNA polymerases, is conserved among eukaryotes and absent from noneukaryotes. Defective mutants were found at an invariant GGLLM motif and at two other...
Cross Talk between tRNA and rRNA Synthesis in Saccharomyces cerevisiae
Briand, Jean-François, Navarro, Francisco, Gadal, Olivier, Thuriaux, Pierre
Temperature-sensitive RNA polymerase III (rpc160-112 and rpc160-270) mutants were analyzed for the synthesis of tRNAs and rRNAs in vivo, using a double-isotopic-labeling technique in which cells are...
τ91, an Essential Subunit of Yeast Transcription Factor IIIC, Cooperates with τ138 in DNA Binding
Arrebola, Rosalía, Manaud, Nathalie, Rozenfeld, Sophie, Marsolier, Marie-Claude, Lefebvre, Olivier, Carles, Christophe, ...
Transcription factor IIIC (TFIIIC) (or τ) is a large multisubunit and multifunctional factor required for transcription of all class III genes in Saccharomyces cerevisiae. It is responsible for...
Hmo1, an HMG-box protein, belongs to the yeast ribosomal DNA transcription system
Gadal, Olivier, Labarre, Sylvie, Boschiero, Claire, Thuriaux, Pierre
Hmo1 is one of seven HMG-box proteins of Saccharo myces cerevisiae. Null mutants have a limited effect on growth. Hmo1 overexpression suppresses rpa49-Δ mutants lacking Rpa49, a non-essential but...
An Rpb4/Rpb7-Like Complex in Yeast RNA Polymerase III Contains the Orthologue of Mammalian CGRP-RCP
Siaut, Magali, Zaros, Cécile, Levivier, Emilie, Ferri, Maria-Laura, Court, Magali, Werner, Michel, ...
The essential C17 subunit of yeast RNA polymerase (Pol) III interacts with Brf1, a component of TFIIIB, suggesting a role for C17 in the initiation step of transcription. The protein sequence of C17...
Members of the SAGA and Mediator complexes are partners of the transcription elongation factor TFIIS
Wery, Maxime, Shematorova, Elena, Van Driessche, Benoît, Vandenhaute, Jean, Thuriaux, Pierre, Van Mullem, Vincent
TFIIS, an elongation factor encoded by DST1 in Saccharomyces cerevisiae, stimulates transcript cleavage in arrested RNA polymerase II. Two components of the RNA polymerase II machinery, Med13 (Srb9)...
A genetic look at the active site of RNA polymerase III
Rozenfeld, Sophie, Thuriaux, Pierre
rpc160-112, a mutant of the RNA polymerase III active site, is corrected in vivo by six second-site mutants obtained by random mutagenesis. These mutants introduce single-site amino acid replacements...
Kohli, Jürg, Hottinger, Herbert, Munz, Peter, Strauss, Andre, Thuriaux, Pierre
The genetic maps of the fission yeast Schizosaccharomyces pombe were extended through the use of haploidization (spontaneous or induced by m-fluorophenylalanine), as well as by tetrad, random spore...
Regulatory Genes Controlling Mitosis in the Fission Yeast SCHIZOSACCHAROMYCES POMBE
Fifty-two wee mutants that undergo mitosis and cell division at a reduced size compared with wild type have been genetically analyzed. The mutants define two genes, wee1 and cdc2, which control the...
Kalogeropoulos, Angelos, Thuriaux, Pierre
A hybrid DNA (hDNA) model of recombination has been algebraically formulated, which allows the prediction of frequencies of postmeiotic segregation and conversion of a given allele and their...
Ancient origin, functional conservation and fast evolution of DNA-dependent RNA polymerase III
Proshkina, Galina M., Shematorova, Elena K., Proshkin, Sergey A., Zaros, Cécile, Thuriaux, Pierre, Shpakovski, George V.
RNA polymerase III contains seventeen subunits in yeasts (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and in human cells. Twelve of them are akin to the core RNA polymerase I or II. The...
Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases
Zaros, Cécile, Briand, Jean-François, Boulard, Yves, Labarre-Mariotte, Sylvie, Garcia-Lopez, M. Carmen, Thuriaux, Pierre, ...
Rpb5, a subunit shared by the three yeast RNA polymerases, combines a eukaryotic N-terminal module with a globular C-end conserved in all non-bacterial enzymes. Conditional and lethal mutants of the...
Two RNA Polymerase I Subunits Control the Binding and Release of Rrn3 during Transcription▿ †
Beckouet, Frédéric, Labarre-Mariotte, Sylvie, Albert, Benjamin, Imazawa, Yukiko, Werner, Michel, Gadal, Olivier, ...
Rpa34 and Rpa49 are nonessential subunits of RNA polymerase I, conserved in species from Saccharomyces cerevisiae and Schizosaccharomyces pombe to humans. Rpa34 bound an N-terminal region of Rpa49 in...