Scientific Commons: Phong Trang

Effective inhibition of human cytomegalovirus gene expression and replication by a ribozyme derived from the catalytic RNA subunit of RNase P from Escherichia coli

Trang, Phong, Lee, Manfred, Nepomuceno, Edward, Kim, Joe, Zhu, Hua, Liu, Fenyong

A sequence-specific ribozyme (M1GS RNA) derived from the catalytic RNA subunit of RNase P from Escherichia coli was used to target the overlapping exon 3 region of the mRNAs encoding the major...

RNase P-mediated inhibition of cytomegalovirus protease expression and viral DNA encapsidation by oligonucleotide external guide sequences

Dunn, Walter, Trang, Phong, Khan, Umair, Zhu, Jiaming, Liu, Fenyong

External guide sequences (EGSs) are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA. In this...

Effective inhibition of herpes simplex virus 1 gene expression and growth by engineered RNase P ribozyme

Trang, Phong, Lee, Jarone, Kilani, Ahmed F., Kim, Joe, Liu, Fenyong

Using an in vitro selection procedure, we have previously isolated ribonuclease P (RNase P) ribozyme variants that efficiently cleave an mRNA sequence in vitro. In this study, an M1GS RNA variant was...

Effective inhibition of Rta expression and lytic replication of Kaposi's sarcoma-associated herpesvirus by human RNase P

Zhu, Jiaming, Trang, Phong, Kim, Kihoon, Zhou, Tianhong, Deng, Hongyu, Liu, Fenyong

Ribonuclease P (RNase P) complexed with external guide sequence (EGS) represents a nucleic acid-based gene interference approach to knock-down gene expression. Unlike other strategies, such as...

RNase P ribozyme inhibits cytomegalovirus replication by blocking the expression of viral capsid proteins

Kim, Kihoon, Trang, Phong, Umamoto, Sean, Hai, Rong, Liu, Fenyong

By linking a guide sequence to the catalytic RNA subunit of RNase P (M1 RNA), we constructed a functional ribozyme (M1GS RNA) that targets the overlapping mRNA region of two human cytomegalovirus...

Dissecting human cytomegalovirus gene function and capsid maturation by ribozyme targeting and electron cryomicroscopy

Yu, Xuekui, Trang, Phong, Shah, Sanket, Atanasov, Ivo, Kim, Yong-Hwan, Bai, Yong, ...

Human CMV (HCMV) is the leading viral cause of birth defects and causes one of the most common opportunistic infections among transplant recipients and AIDS patients. Cleavage of internal scaffolding...

Intracellular expression of engineered RNase P ribozymes effectively blocks gene expression and replication of human cytomegalovirus

KIM, KIHOON, UMAMOTO, SEAN, TRANG, PHONG, HAI, RONG, LIU, FENYONG

A ribozyme (M1GS RNA) constructed from the catalytic RNA subunit of RNase P from Escherichia coli was used to target the overlapping region of two human cytomegalovirus (HCMV) mRNAs, which encode for...

Effective inhibition of human cytomegalovirus gene expression and growth by intracellular expression of external guide sequence RNA

LI, HONGJIAN, TRANG, PHONG, KIM, KIHOON, ZHOU, TIANHONG, UMAMOTO, SEAN, LIU, FENYONG

RNase P complexed with external guide sequence (EGS) represents a novel nucleic-acid-based gene interference approach to modulate gene expression. In this study, a functional EGS RNA was constructed...