Prof. Dr. Rer. Nat. Petra Schwille
The progress of miniaturisation towards the nanoscopic scale in science and technology has also influenced the biosciences. This is particularly important, since proteins, as the smallest functional...
Supercritical angle fluorescence correlation spectroscopy (2007)
Ries, Jonas, Ruckstuhl, Thomas, Verdes, Dorinel, Schwille, Petra
We explore the potential of a supercritical angle (SA) objective for fluorescence correlation spectroscopy (FCS). This novel microscope objective combines tight focusing by an aspheric lens with...
Becker, Christian F. W., Seidel, Ralf, Jahnz, Michael, Bacia, Kirsten, Niederhausen, Thomas, Alexandrov, Kirill, ...
Poolman, Bert, Bacia, Kirsten, Scherfeld, Dag, Kahya, Nicoletta, Schwille, Petra
Confocal fluorescence microscopy and fluorescence correlation spectroscopy (FCS) have been employed to investigate the lipid spatial and dynamic organization in giant unilamellar vesicles (GUVs)...
Zugl.: Braunschweig, Techn. Univ., Diss., 1996.
Korlach, Jonas, Schwille, Petra, Webb, Watt W., Feigenson, Gerald W.
We report the application of confocal imaging and fluorescence correlation spectroscopy (FCS) to characterize chemically well-defined lipid bilayer models for biomembranes. Giant unilamellar vesicles...
Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy
Kettling, Ulrich, Koltermann, Andre, Schwille, Petra, Eigen, Manfred
A method for sensitively monitoring enzyme kinetics and activities by using dual-color fluorescence cross-correlation spectroscopy is described. This universal method enables the development of...
Walter, Nils G., Schwille, Petra, Eigen, Manfred
A sensitive, labor-saving, and easily automatable nonradioactive procedure named APEX-FCS (amplified probe extension detected by fluorescence correlation spectroscopy) has been established to detect...
Oehlenschläger, Frank, Schwille, Petra, Eigen, Manfred
Nucleic acid sequence-based amplification (NASBA) has proved to be an ultrasensitive method for HIV-1 diagnosis in plasma even in the primary HIV infection stage. This technique was combined with...
Haupts, Ulrich, Maiti, Sudipta, Schwille, Petra, Webb, Watt W.
We have investigated the pH dependence of the dynamics of conformational fluctuations of green fluorescent protein mutants EGFP (F64L/S65T) and GFP-S65T in small ensembles of molecules in solution by...
Schwille, Petra, Kummer, Susanne, Heikal, Ahmed A., Moerner, W. E., Webb, Watt W.
Fast excitation-driven fluctuations in the fluorescence emission of yellow-shifted green fluorescent protein mutants T203Y and T203F, with S65G/S72A, are discovered in the 10−6–10−3-s time...
Heinze, Katrin G., Koltermann, Andre, Schwille, Petra
Confocal fluorescence correlation spectroscopy as a time-averaging fluctuation analysis combining maximum sensitivity with high statistical confidence has proved to be a very versatile and powerful...
Kohl, Tobias, Heinze, Katrin G., Kuhlemann, Rene, Koltermann, Andre, Schwille, Petra
GFP and the red fluorescent protein, DsRed, have been combined to design a protease assay that allows not only for fluorescence resonance energy transfer (FRET) studies but also for dual-color...
Intracellular calmodulin availability accessed with two-photon cross-correlation
Kim, Sally A., Heinze, Katrin G., Waxham, M. Neal, Schwille, Petra
The availability and interactions of signaling proteins are tightly regulated in time and space to produce specific and localized effects. For calmodulin (CaM), a key transducer of intracellular Ca2+...
Bacia, Kirsten, Schwille, Petra, Kurzchalia, Teymuras
The existence of lipid rafts in biological membranes in vivo is still debated. In contrast, the formation of domains in model systems has been well documented. In giant unilamellar vesicles (GUVs)...
Jahnz, Michael, Schwille, Petra
Site-specific exchange of genetic information is mediated by DNA recombinases, such as FLP or Cre, and has become a valuable tool in modern molecular biology. The so far low number of suitable...
Bacia, Kirsten, Majoul, Irina V, Schwille, Petra
Fluorescence (auto)correlation spectroscopy (FCS) has developed into a widely used method for investigating molecular dynamics and mobility of molecules in vitro and in vivo. Dual-color...
Two-photon fluorescence coincidence analysis: rapid measurements of enzyme kinetics.
Heinze, Katrin G, Rarbach, Markus, Jahnz, Michael, Schwille, Petra
Dual-color fluorescence cross-correlation analysis is a powerful tool for probing interactions of different fluorescently labeled molecules in aqueous solution. The concept is the selective...
Scherfeld, Dag, Kahya, Nicoletta, Schwille, Petra
In recent years, the implication of sphingomyelin in lipid raft formation has intensified the long sustained interest in this membrane lipid. Accumulating evidences show that cholesterol...
Heinze, Katrin G., Jahnz, Michael, Schwille, Petra
Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system....
Fluorescence Correlation Spectroscopy Relates Rafts in Model and Native Membranes
Bacia, Kirsten, Scherfeld, Dag, Kahya, Nicoletta, Schwille, Petra
The lipid raft model has evoked a new perspective on membrane biology. Understanding the structure and dynamics of lipid domains could be a key to many crucial membrane-associated processes in cells....
Böse, Guido, Schwille, Petra, Lamparter, Tilman
Fluorescence correlation spectroscopy (FCS) is a versatile tool for investigating the mobilities of fluorescent molecules in cells. In this article, we show that it is possible to distinguish between...
Gordon, Sean Patrick, Berezhna, Svitlana, Scherfeld, Dag, Kahya, Nicoletta, Schwille, Petra
Complexes formed by cationic liposomes and single-strand oligodeoxynucleotides (CL-ODN) are promising delivery systems for antisense therapy. ODN release from the complexes is an essential step for...
In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5
Ohrt, Thomas, Merkle, Dennis, Birkenfeld, Karin, Echeverri, Christophe J., Schwille, Petra
Two types of short double-stranded RNA molecules, namely microRNAs (miRNAs) and short interfering RNAs (siRNAs), have emerged recently as important regulators of gene expression. Although these...
Two-Photon Cross-Correlation Analysis of Intracellular Reactions with Variable Stoichiometry
Kim, Sally A., Heinze, Katrin G., Bacia, Kirsten, Waxham, M. Neal, Schwille, Petra
We successfully demonstrate the effectiveness of two-photon fluorescence cross-correlation spectroscopy (TPCCS) to study the complex binding stoichiometry of calmodulin (CaM) and Ca2+/CaM-dependent...
Determining Protease Activity In Vivo by Fluorescence Cross-Correlation Analysis
Kohl, Tobias, Haustein, Elke, Schwille, Petra
To date, most biochemical approaches to unravel protein function have focused on purified proteins in vitro. Whereas they analyze enzyme performance under assay conditions, they do not necessarily...
Korlach, Jonas, Schwille, Petra, Webb, Watt W., Feigenson, Gerald W.
We report the application of confocal imaging and fluorescence correlation spectroscopy (FCS) to characterize chemically well-defined lipid bilayer models for biomembranes. Giant unilamellar vesicles...
Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy
Kettling, Ulrich, Koltermann, Andre, Schwille, Petra, Eigen, Manfred
A method for sensitively monitoring enzyme kinetics and activities by using dual-color fluorescence cross-correlation spectroscopy is described. This universal method enables the development of...
Walter, Nils G., Schwille, Petra, Eigen, Manfred
A sensitive, labor-saving, and easily automatable nonradioactive procedure named APEX-FCS (amplified probe extension detected by fluorescence correlation spectroscopy) has been established to detect...
Oehlenschläger, Frank, Schwille, Petra, Eigen, Manfred
Nucleic acid sequence-based amplification (NASBA) has proved to be an ultrasensitive method for HIV-1 diagnosis in plasma even in the primary HIV infection stage. This technique was combined with...
Haupts, Ulrich, Maiti, Sudipta, Schwille, Petra, Webb, Watt W.
We have investigated the pH dependence of the dynamics of conformational fluctuations of green fluorescent protein mutants EGFP (F64L/S65T) and GFP-S65T in small ensembles of molecules in solution by...
Schwille, Petra, Kummer, Susanne, Heikal, Ahmed A., Moerner, W. E., Webb, Watt W.
Fast excitation-driven fluctuations in the fluorescence emission of yellow-shifted green fluorescent protein mutants T203Y and T203F, with S65G/S72A, are discovered in the 10−6–10−3-s time...
Heinze, Katrin G., Koltermann, Andre, Schwille, Petra
Confocal fluorescence correlation spectroscopy as a time-averaging fluctuation analysis combining maximum sensitivity with high statistical confidence has proved to be a very versatile and powerful...
Kohl, Tobias, Heinze, Katrin G., Kuhlemann, Rene, Koltermann, Andre, Schwille, Petra
GFP and the red fluorescent protein, DsRed, have been combined to design a protease assay that allows not only for fluorescence resonance energy transfer (FRET) studies but also for dual-color...
Intracellular calmodulin availability accessed with two-photon cross-correlation
Kim, Sally A., Heinze, Katrin G., Waxham, M. Neal, Schwille, Petra
The availability and interactions of signaling proteins are tightly regulated in time and space to produce specific and localized effects. For calmodulin (CaM), a key transducer of intracellular Ca2+...
Bacia, Kirsten, Schwille, Petra, Kurzchalia, Teymuras
The existence of lipid rafts in biological membranes in vivo is still debated. In contrast, the formation of domains in model systems has been well documented. In giant unilamellar vesicles (GUVs)...
Jahnz, Michael, Schwille, Petra
Site-specific exchange of genetic information is mediated by DNA recombinases, such as FLP or Cre, and has become a valuable tool in modern molecular biology. The so far low number of suitable...
Bacia, Kirsten, Majoul, Irina V, Schwille, Petra
Fluorescence (auto)correlation spectroscopy (FCS) has developed into a widely used method for investigating molecular dynamics and mobility of molecules in vitro and in vivo. Dual-color...
Two-photon fluorescence coincidence analysis: rapid measurements of enzyme kinetics.
Heinze, Katrin G, Rarbach, Markus, Jahnz, Michael, Schwille, Petra
Dual-color fluorescence cross-correlation analysis is a powerful tool for probing interactions of different fluorescently labeled molecules in aqueous solution. The concept is the selective...
Scherfeld, Dag, Kahya, Nicoletta, Schwille, Petra
In recent years, the implication of sphingomyelin in lipid raft formation has intensified the long sustained interest in this membrane lipid. Accumulating evidences show that cholesterol...
Heinze, Katrin G., Jahnz, Michael, Schwille, Petra
Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system....
Fluorescence Correlation Spectroscopy Relates Rafts in Model and Native Membranes
Bacia, Kirsten, Scherfeld, Dag, Kahya, Nicoletta, Schwille, Petra
The lipid raft model has evoked a new perspective on membrane biology. Understanding the structure and dynamics of lipid domains could be a key to many crucial membrane-associated processes in cells....
Böse, Guido, Schwille, Petra, Lamparter, Tilman
Fluorescence correlation spectroscopy (FCS) is a versatile tool for investigating the mobilities of fluorescent molecules in cells. In this article, we show that it is possible to distinguish between...
Gordon, Sean Patrick, Berezhna, Svitlana, Scherfeld, Dag, Kahya, Nicoletta, Schwille, Petra
Complexes formed by cationic liposomes and single-strand oligodeoxynucleotides (CL-ODN) are promising delivery systems for antisense therapy. ODN release from the complexes is an essential step for...
Two-Photon Cross-Correlation Analysis of Intracellular Reactions with Variable Stoichiometry
Kim, Sally A., Heinze, Katrin G., Bacia, Kirsten, Waxham, M. Neal, Schwille, Petra
We successfully demonstrate the effectiveness of two-photon fluorescence cross-correlation spectroscopy (TPCCS) to study the complex binding stoichiometry of calmodulin (CaM) and Ca2+/CaM-dependent...
Determining Protease Activity In Vivo by Fluorescence Cross-Correlation Analysis
Kohl, Tobias, Haustein, Elke, Schwille, Petra
To date, most biochemical approaches to unravel protein function have focused on purified proteins in vitro. Whereas they analyze enzyme performance under assay conditions, they do not necessarily...
In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5
Ohrt, Thomas, Merkle, Dennis, Birkenfeld, Karin, Echeverri, Christophe J., Schwille, Petra
Two types of short double-stranded RNA molecules, namely microRNAs (miRNAs) and short interfering RNAs (siRNAs), have emerged recently as important regulators of gene expression. Although these...
Effects of Ceramide on Liquid-Ordered Domains Investigated by Simultaneous AFM and FCS
Chiantia, Salvatore, Kahya, Nicoletta, Ries, Jonas, Schwille, Petra
The sphingolipid ceramides are known to influence lipid lateral organization in biological membranes. In particular, ceramide-induced alterations of microdomains can be involved in several cell...
Studying Slow Membrane Dynamics with Continuous Wave Scanning Fluorescence Correlation Spectroscopy
Here we discuss the application of scanning fluorescence correlation spectroscopy (SFCS) using continuous wave excitation to analyze membrane dynamics. The high count rate per molecule enables the...
Phosphatidylethanolamine critically supports internalization of cell-penetrating protein C inhibitor
Baumgärtner, Petra, Geiger, Margarethe, Zieseniss, Susanne, Malleier, Julia, Huntington, James A., Hochrainer, Karin, ...
Although their contribution remains unclear, lipids may facilitate noncanonical routes of protein internalization into cells such as those used by cell-penetrating proteins. We show that protein C...
Pore Formation by a Bax-Derived Peptide: Effect on the Line Tension of the Membrane Probed by AFM
García-Sáez, Ana J., Chiantia, Salvatore, Salgado, Jesús, Schwille, Petra
Bax is a critical regulator of physiological cell death that increases the permeability of the outer mitochondrial membrane and facilitates the release of the so-called apoptotic factors during...
Ohrt, Thomas, Mütze, Jörg, Staroske, Wolfgang, Weinmann, Lasse, Höck, Julia, Crell, Karin, ...
Studies of RNA interference (RNAi) provide evidence that in addition to the well-characterized cytoplasmic mechanisms, nuclear mechanisms also exist. The mechanism by which the nuclear RNA-induced...