Chromosome 20q Amplification Regulates in Vitro Response to Kinesin-5 Inhibitor (2008)
Aimee L. Jackson, Mao Mao, Sumire Kobayashi, Teresa Ward, Matthew Biery, Hongyue Dai, ...
We identified gene expression signatures predicting responsiveness to a Kinesin-5 (KIF11) inhibitor (Kinesin-5i) in cultured colon tumor cell lines. Genes predicting resistance to Kinesin-5i were...
Chromosome 20q Amplification Regulates in Vitro Response to Kinesin-5 Inhibitor (2008)
Aimee L. Jackson, Mao Mao, Sumire Kobayashi, Teresa Ward, Matthew Biery, Hongyue Dai, ...
We identified gene expression signatures predicting responsiveness to a Kinesin-5 (KIF11) inhibitor (Kinesin-5i) in cultured colon tumor cell lines. Genes predicting resistance to Kinesin-5i were...
Xudong Dai, Hongyue Dai, David L. Lewis, Chang-kyu Lee, Andy G. Spencer, ...
Uncovering pathways underlying drug-induced toxicity is a fundamental objective in the field of toxicogenomics. Developing mechanism-based toxicity biomarkers requires the identification of such...
Xudong Dai, Hongyue Dai, David L. Lewis, Chang-kyu Lee, Andy G. Spencer, ...
Uncovering pathways underlying drug-induced toxicity is a fundamental objective in the field of toxicogenomics. Developing mechanism-based toxicity biomarkers requires the identification of such...
Designing siRNA That Distinguish between Genes That Differ by a Single Nucleotide (2006)
Dianne S. Schwarz, Hongliu Ding, Lori Kennington, Jessica T. Moore, Janell Schelter, Julja Burchard, ...
Small interfering RNAs (siRNAs), the guides that direct RNA interference (RNAi), provide a powerful tool to reduce the expression of a single gene in human cells. Ideally, dominant, gain-of-function...
Designing siRNA that distinguish between genes that differ by a single nucleotide (2006)
Dianne S Schwarz, Hongliu D Ding, Lori Kennington, Jessica T Moore, Janell Schelter, Julja Burchard, ...
Small interfering RNAs (siRNAs), the guides that direct RNA interference (RNAi), provide a powerful tool to reduce the expression of a single gene in human cells. Ideally, dominant, gain-of-function...
Minimizing the risk of reporting false positives in large-scale RNAi screens (2006)
Echeverri, Christophe J, Beachy, Philip A, Baum, Buzz, Boutros, Michael, Buchholz, Frank, Chanda, Sumit K, ...
Large-scale RNA interference (RNAi)-based analyses, very much as other 'omic' approaches, have inherent rates of false positives and negatives. The variability in the standards of care applied to...
Role of the CD28 receptor in T-cell activation (1990)
June, Carl H., Ledbetter, Jeffrey A., Linsley, Peter S., Thompson, Craig B.
Antigen-specific T-cell activation is initiated through the T-cell receptor. Recent evidence has shown that a number of additional T-cell surface receptors serve to regulate the responses of...
Villegas, Eric N., Wille, Ulrike, Craig, Linden, Linsley, Peter S., Rennick, Donna M., Peach, Robert, ...
Interleukin-10 (IL-10) is associated with inhibition of cell-mediated immunity and downregulation of the expression of costimulatory molecules required for T-cell activation. When IL-10-deficient...
Modulation of TCR-induced transcriptional profiles by ligation of CD28, ICOS, and CTLA-4 receptors
Riley, James L., Mao, Mao, Kobayashi, Sumire, Biery, Matt, Burchard, Julja, Cavet, Guy, ...
Signals generated by T cell receptor (TCR) and CD28 engagement are required for optimal T cell activation, but how these signals integrate within the cell is still largely unknown. We have used near...
CTLA4Ig-mediated blockade of T-cell costimulation in patients with psoriasis vulgaris
Abrams, Judith R., Lebwohl, Mark G., Guzzo, Cynthia A., Jegasothy, Brian V., Goldfarb, Michael T., Goffe, Bernard S., ...
Engagement of the B7 family of molecules on antigen-presenting cells with their T cell–associated ligands, CD28 and CD152 (cytotoxic T lymphocyte–associated antigen-4 [CTLA-4]), provides a...
CTLA-4 and PD-1 Receptors Inhibit T-Cell Activation by Distinct Mechanisms†
Parry, Richard V., Chemnitz, Jens M., Frauwirth, Kenneth A., Lanfranco, Anthony R., Braunstein, Inbal, Kobayashi, Sumire V., ...
CTLA-4 and PD-1 are receptors that negatively regulate T-cell activation. Ligation of both CTLA-4 and PD-1 blocked CD3/CD28-mediated upregulation of glucose metabolism and Akt activity, but each...
Gene expression changes associated with progression and response in chronic myeloid leukemia
Radich, Jerald P., Dai, Hongyue, Mao, Mao, Oehler, Vivian, Schelter, Jan, Druker, Brian, ...
Chronic myeloid leukemia (CML) is a hematopoietic stem cell disease with distinct biological and clinical features. The biologic basis of the stereotypical progression from chronic phase through...
Carleton, Michael, Mao, Mao, Biery, Matthew, Warrener, Paul, Kim, Sammy, Buser, Carolyn, ...
KIF14 is a microtubule motor protein whose elevated expression is associated with poor-prognosis breast cancer. Here we demonstrate KIF14 accumulation in mitotic cells, where it associated with...
Designing siRNA That Distinguish between Genes That Differ by a Single Nucleotide
Schwarz, Dianne S, Ding, Hongliu, Kennington, Lori, Moore, Jessica T, Schelter, Janell, Burchard, Julja, ...
Small interfering RNAs (siRNAs), the guides that direct RNA interference (RNAi), provide a powerful tool to reduce the expression of a single gene in human cells. Ideally, dominant, gain-of-function...
Transcriptional and phenotypic comparisons of Ppara knockout and siRNA knockdown mice
De Souza, Angus T., Dai, Xudong, Spencer, Andrew G., Reppen, Tom, Menzie, Ann, Roesch, Paula L., ...
RNA interference (RNAi) has great potential as a tool for studying gene function in mammals. However, the specificity and magnitude of the in vivo response to RNAi remains to be fully characterized....
Villegas, Eric N., Wille, Ulrike, Craig, Linden, Linsley, Peter S., Rennick, Donna M., Peach, Robert, ...
Interleukin-10 (IL-10) is associated with inhibition of cell-mediated immunity and downregulation of the expression of costimulatory molecules required for T-cell activation. When IL-10-deficient...
Modulation of TCR-induced transcriptional profiles by ligation of CD28, ICOS, and CTLA-4 receptors
Riley, James L., Mao, Mao, Kobayashi, Sumire, Biery, Matt, Burchard, Julja, Cavet, Guy, ...
Signals generated by T cell receptor (TCR) and CD28 engagement are required for optimal T cell activation, but how these signals integrate within the cell is still largely unknown. We have used near...
CTLA4Ig-mediated blockade of T-cell costimulation in patients with psoriasis vulgaris
Abrams, Judith R., Lebwohl, Mark G., Guzzo, Cynthia A., Jegasothy, Brian V., Goldfarb, Michael T., Goffe, Bernard S., ...
Engagement of the B7 family of molecules on antigen-presenting cells with their T cell–associated ligands, CD28 and CD152 (cytotoxic T lymphocyte–associated antigen-4 [CTLA-4]), provides a...
CTLA-4 and PD-1 Receptors Inhibit T-Cell Activation by Distinct Mechanisms†
Parry, Richard V., Chemnitz, Jens M., Frauwirth, Kenneth A., Lanfranco, Anthony R., Braunstein, Inbal, Kobayashi, Sumire V., ...
CTLA-4 and PD-1 are receptors that negatively regulate T-cell activation. Ligation of both CTLA-4 and PD-1 blocked CD3/CD28-mediated upregulation of glucose metabolism and Akt activity, but each...
Gene expression changes associated with progression and response in chronic myeloid leukemia
Radich, Jerald P., Dai, Hongyue, Mao, Mao, Oehler, Vivian, Schelter, Jan, Druker, Brian, ...
Chronic myeloid leukemia (CML) is a hematopoietic stem cell disease with distinct biological and clinical features. The biologic basis of the stereotypical progression from chronic phase through...
Carleton, Michael, Mao, Mao, Biery, Matthew, Warrener, Paul, Kim, Sammy, Buser, Carolyn, ...
KIF14 is a microtubule motor protein whose elevated expression is associated with poor-prognosis breast cancer. Here we demonstrate KIF14 accumulation in mitotic cells, where it associated with...
Designing siRNA That Distinguish between Genes That Differ by a Single Nucleotide
Schwarz, Dianne S, Ding, Hongliu, Kennington, Lori, Moore, Jessica T, Schelter, Janell, Burchard, Julja, ...
Small interfering RNAs (siRNAs), the guides that direct RNA interference (RNAi), provide a powerful tool to reduce the expression of a single gene in human cells. Ideally, dominant, gain-of-function...
Transcriptional and phenotypic comparisons of Ppara knockout and siRNA knockdown mice
De Souza, Angus T., Dai, Xudong, Spencer, Andrew G., Reppen, Tom, Menzie, Ann, Roesch, Paula L., ...
RNA interference (RNAi) has great potential as a tool for studying gene function in mammals. However, the specificity and magnitude of the in vivo response to RNAi remains to be fully characterized....
Position-specific chemical modification of siRNAs reduces “off-target” transcript silencing
Jackson, Aimee L., Burchard, Julja, Leake, Devin, Reynolds, Angela, Schelter, Janell, Guo, Jie, ...
Transfected siRNAs regulate numerous transcripts sharing limited complementarity to the RNA duplex. This unintended (“off-target”) silencing can hinder the use of RNAi to define gene function....
Widespread siRNA “off-target” transcript silencing mediated by seed region sequence complementarity
Jackson, Aimee L., Burchard, Julja, Schelter, Janell, Chau, B. Nelson, Cleary, Michele, Lim, Lee, ...
Transfected siRNAs and miRNAs regulate numerous transcripts that have only limited complementarity to the active strand of the RNA duplex. This process reflects natural target regulation by miRNAs,...
Bartz, Steven R., Zhang, Zhan, Burchard, Julja, Imakura, Maki, Martin, Melissa, Palmieri, Anthony, ...
RNA interference technology allows the systematic genetic analysis of the molecular alterations in cancer cells and how these alterations affect response to therapies. Here we used small interfering...
Dai, Xudong, Souza, Angus T. De, Dai, Hongyue, Lewis, David L, Lee, Chang-kyu, Spencer, Andy G, ...
Uncovering pathways underlying drug-induced toxicity is a fundamental objective in the field of toxicogenomics. Developing mechanism-based toxicity biomarkers requires the identification of such...
Transcripts Targeted by the MicroRNA-16 Family Cooperatively Regulate Cell Cycle Progression▿ †
Linsley, Peter S., Schelter, Janell, Burchard, Julja, Kibukawa, Miho, Martin, Melissa M., Bartz, Steven R., ...
microRNAs (miRNAs) are abundant, ∼21-nucleotide, noncoding regulatory RNAs. Each miRNA may regulate hundreds of mRNA targets, but the identities of these targets and the processes they regulate are...
Abrams, Judith R., Kelley, Susan L., Hayes, Elizabeth, Kikuchi, Toyoko, Brown, Michael J., Kang, Sewon, ...
Efficient T cell activation is dependent on the intimate contact between antigen-presenting cells (APCs) and T cells. The engagement of the B7 family of molecules on APCs with CD28 and CD152...
CD80 Costimulation Is Essential for the Induction of Airway Eosinophilia
Harris, Nicola, Peach, Robert, Naemura, Joe, Linsley, Peter S., Le Gros, Graham, Ronchese, Franca
CD80 and CD86 (B7-1 and B7-2) are the ligands on antigen-presenting cells (APCs) which bind CD28 and deliver the costimulatory signals necessary for T cell activation. The reasons for the existence...
MicroRNAs in the miR-106b Family Regulate p21/CDKN1A and Promote Cell Cycle Progression▿ †
Ivanovska, Irena, Ball, Alexey S., Diaz, Robert L., Magnus, Jill F., Kibukawa, Miho, Schelter, Janell M., ...
microRNAs in the miR-106b family are overexpressed in multiple tumor types and are correlated with the expression of genes that regulate the cell cycle. Consistent with these observations, miR-106b...