Rubins, Kathleen H, Hensley, Lisa E, Wahl-Jensen, Victoria, Daddario DiCaprio, Kathleen M, Young, Howard A, Reed, Douglas S, ...
Abstract Background Infection with Ebola virus (EBOV) causes a fulminant and often fatal hemorrhagic fever. In order to improve our understanding of EBOV pathogenesis and EBOV-host interactions, we...
Neutralizing Antibody Fails to Impact the Course of Ebola Virus Infection in Monkeys (2007)
Wendelien B. Oswald, Thomas W. Geisbert, Kelly J. Davis, Joan B. Geisbert, Nancy J. Sullivan, Peter B. Jahrling, ...
Prophylaxis with high doses of neutralizing antibody typically offers protection against challenge with viruses producing acute infections. In this study, we have investigated the ability of the...
Nancy J. Sullivan, Thomas W. Geisbert, Joan B. Geisbert, Devon J. Shedlock, Ling Xu, Laurie Lamoreaux, ...
A simplified Ebola vaccine that consists of a modified GP protein (which is well-tolerated by human cells even at high concentrations) in a replication-defective adenoviral vector protects macaques.
Nancy J. Sullivan, Thomas W. Geisbert, Joan B. Geisbert, Devon J. Shedlock, Ling Xu, Laurie Lamoreaux, ...
BackgroundEbola virus causes a hemorrhagic fever syndrome that is associated with high mortality in humans. In the absence of effective therapies for Ebola virus infection, the development of a...
Cynomolgus Macaque as an Animal Model for Severe Acute Respiratory Syndrome (2006)
James V. Lawler, Timothy P. Endy, Lisa E. Hensley, Aura Garrison, Elizabeth A. Fritz, May Lesar, ...
Jason Paragas and colleagues report the first findings of measurable clinical disease in nonhuman primates infected with the virus that causes SARS, SARS-CoV.
Cynomolgus Macaque as an Animal Model for Severe Acute Respiratory Syndrome (2006)
James V. Lawler, Timothy P. Endy, Lisa E. Hensley, Aura Garrison, Elizabeth A. Fritz, May Lesar, ...
BackgroundThe emergence of severe acute respiratory syndrome (SARS) in 2002 and 2003 affected global health and caused major economic disruption. Adequate animal models are required to study the...
Masstag polymerase chain reaction for differential diagnosis of viral hemorrhagic fevers (2006)
Palacios, Gustavo, Briese, Thomas, Kapoor, Vishal, Jabado, Omar, Liu, Zhiqiang, Venter, Marietjie, ...
Viral hemorrhagic fevers are associated with high rates of illness and death. Although therapeutic options are limited, early differential diagnosis has implications for containment and may aid in...
Development of a New Vaccine for the Prevention of Lassa Fever (2005)
Thomas W. Geisbert, Steven Jones, Elizabeth A. Fritz, Amy C. Shurtleff, Joan B. Geisbert, Ryan Liebscher, ...
A replication-competent vaccine based on attenuated recombinant vesicular stomatitis virus vector protects non- human primates.
Development of a New Vaccine for the Prevention of Lassa Fever (2005)
Thomas W. Geisbert, Steven Jones, Elizabeth A. Fritz, Amy C. Shurtleff, Joan B. Geisbert, Ryan Liebscher, ...
Background Recent importation of Lassa fever into Germany, the Netherlands, the United Kingdom, and the United States by travelers on commercial airlines from Africa underscores the public health...
Interferon-Beta 1a and SARS Coronavirus Replication (2004)
Hensley, Lisa E ., Fritz, Elizabeth A., Jahrling, Peter B., Karp, Christopher L., Huggins, John W.
A global outbreak of severe acute respiratory syndrome (SARS) caused by a novel coronavirus began in March 2003. The rapid emergence of SARS and the substantial illness and death it caused have made...
Receptor Interaction between Eastern Equine Encephalitis Virus and Chick Embryo Fibroblasts. (2002)
Marker,Stephen C., Connelly,Donald, Jahrling,Peter B.
The attachment of eastern equine encephalitis virus to chick embryo fibroblasts was studied at 0 C. The binding specifically responsible for initiating infection was studied in the initial...
Jahrling,Peter B., Heisey,Gregory B., Hesse,Richard A.
In the present study, using guinea pigs, six of six virulent strains of Venezuelan encephalitis virus (VEE) were cleared inefficiently, while three of six nonlethal or benign virus strains were...
Beisel,William R., Jahrling,Peter B., Levitt,Neil H., Powanda,Michael C., Mapes,Carol A.
Contents: The current status of USAMRIID and its research activities; Relation of virion surface charges to virulence of arboviruses; Virus replication in peripheral human leukocytes; Systemic...
Enterotoxic Shock in Rhesus Monkeys: The Role of Selected Blood-Borne Factors. (2002)
Pettit,George W., Yamada,Tadataka, Wing,David A., Jahrling,Peter B.
Staphylococcal enterotoxin B, a protein exotoxin from Staphylococcus aureus, produced progressive hypotension and shock when injected (1 mg/kg, intravenous) into rhesus monkeys. Plasma levels of...
Effect of Ribavirin on Protein and RNA Synthesis in VEE Virus-Infected Cells. (2002)
Canonico,Peter G., Little,James S., Jahrling,Peter B., Stephen,Edward L.
Ribavirin is a nucleoside analogue with antiviral activity against the etiologic agents of such militarily important diseases as Lassa fever, Bolivian and Argentinian hemorrhagic fevers, Rift Valley...
Mechanism of Action of Ribavirin: An Antiviral Drug of Military Importance, (2002)
Canonico,Peter G., Little,James S., Jahrling,Peter B., Stephen,Edward L.
Ribavirin (1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide) is a nucleoside analogue having broad spectrum antiviral activity against both DNA and RNA viruses (18). A variety of specific effects...
Neufeld,Harold A., Pace,Judith G., Kaminski,Mitchell V., George,David T., Jahrling,Peter B.
Supersedes report dated 2 Jul 79, AD-A072 139.
Pathogenesis of Lassa Virus Infection in Guinea Pigs, (2002)
Jahrling,Peter B., Smith,Sheilda, Hesse,Richard A., Rhoderick,Joan B.
Pathology of Lassa Virus Infection in the Rhesus Monkey, (2002)
Callis,Robert T., Jahrling,Peter B., DePaoli,Alexander
Supersedes report dated 12 Feb 81, AD-A095 994.
Opsonization of Alphaviruses in Hamsters, (1998)
Jahrling,Peter B., Hesse,Richard A., Anderson,Arthur O., Gangemi,J. David
Cosgriff,Thomas M., Jahrling,Peter B., Chen,James P., Hodgson,Loreen A., Lewis,Richard M.
Significant coagulation abnormalities were associated with experimental infection of strain 13 guinea pigs with Pichinde virus, an arenavirus related to the virulent human pathogens Junin, Machupo,...
Rosato, Robert R., Macasaet, Francisco F., Jahrling, Peter B.
Enzyme-linked immunosorbent assays (ELISAs) were developed for detection of immunoglobulin G (IgG) and IgM antibodies to Venezuelan equine encephalomyelitis (VEE) virus in vaccinated and naturally...
Immune Serum Increases Arenavirus Replication in Monocytes, (1998)
Lewis, Richard M., Cosgriff, Thomas M., Griffin, Beverly Y., Rhoderick, Joan, Jahrling, Peter B.
The U937 monocytic cell line was used to determine whether antibodies could facilitate infection and replication of the arenaviruses, Pichinde virus (PV) and Lassa fever virus (LFV). When high...
Filovirus Pathogenesis in Nonhuman Primates (1998)
Geisbert, Thomas W., Jahrling, Peter B., Larsen, Tom, Davis, Kelly J., Hensley, Lisa
Infections with Ebola and Marburg viruses cause severe and fatal hemorrhagic disease in humans and nonhuman primates. While progress to define the mechanisms of filoviral pathogenesis has been made...
Towards a Vaccine Against Ebola Virus (1998)
Geisbert, Thomas W., Jahrling, Peter B.
Ebola virus infection causes hemorrhagic fever with high mortality rates in humans and nonhuman primates. Currently, there are no vaccines or therapies approved for human use. Outbreaks of Ebola...
Jahrling, Peter B., Hensley, Lisa E., Martinez, Mark J., LeDuc, James W., Rubins, Kathleen H.
Smallpox virus (variola) poses a significant threat as an agent of bioterrorism. To mitigate this risk, antiviral drugs and an improved vaccine are urgently needed. Satisfactory demonstration of...
Geisbert, Thomas W., Hensley, Lisa E., Jahrling, Peter B., Larsen, Tom, Geisbert, Joan B.
Infection with the Ebola virus induces overexpression of the procoagulant tissue factor in primate monocytes and macrophages, suggesting that inhibition of the tissue-factor pathway could ameliorate...
Jones, Steven M., Feldmann, Heinz, Stroher, Ute, Geisbert, Joan B., Fernando, Lisa, Grolla, Allen, ...
Vaccines and therapies are urgently needed to address public health needs stemming from emerging pathogens and biological threat agents such as the filoviruses Ebola virus (EBOV) and Marburg virus...
Daddario-DiCaprio, Kathleen M., Geisbert, Thomas W., Stroher, Ute, Geisbert, Joan B., Grolla, Allen, Fritz, Elizabeth A., ...
Effective countermeasures are urgently needed to prevent and treat infections caused by highly pathogenic and biological threat agents such as Marburg virus (MARV). We aimed to test the efficacy of a...
MassTag Polymerase Chain Reaction for Differential Diagnosis of Viral Hemorrhagic Fevers (1998)
Palacios, Gustavo, Briese, Thomas, Kapoor, Vishal, Jabado, Omar, Liu, Zhiqiang, Venter, Marietjie, ...
Viral hemorrhagic fevers are associated with high rates of illness and death. Although therapeutic options are limited, early differential diagnosis has implications for containment and may aid in...
Ebola Virus Can Be Effectively Neutralized by Antibody Produced in Natural Human Infection
Maruyama, Toshiaki, Rodriguez, Luis L., Jahrling, Peter B., Sanchez, Anthony, Khan, Ali S., Nichol, Stuart T., ...
The activity of antibodies against filoviruses is poorly understood but has important consequences for vaccine design and passive prophylaxis. To investigate this activity, a panel of recombinant...
Geisbert, Tom W., Maruyama, Toshiaki, Jahrling, Peter B., Burton, Dennis R.
A neutralizing human monoclonal antibody, KZ52, protects guinea pigs from lethal Ebola Zaire virus challenge. Administration before or up to 1 h after challenge resulted in dose-dependent protection...
Real-Time PCR Assay To Detect Smallpox Virus
Sofi Ibrahim, M., Kulesh, David A., Saleh, Sharron S., Damon, Inger K., Esposito, Joseph J., Schmaljohn, Alan L., ...
We developed a highly sensitive and specific assay for the rapid detection of smallpox virus DNA on both the Smart Cycler and LightCycler platforms. The assay is based on TaqMan chemistry with the...
Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus
Yount, Boyd, Curtis, Kristopher M., Fritz, Elizabeth A., Hensley, Lisa E., Jahrling, Peter B., Prentice, Erik, ...
A previously undescribed coronavirus (CoV) is the etiologic agent responsible for severe acute respiratory syndrome (SARS). Using a panel of contiguous cDNAs that span the entire genome, we have...
Radioimmunoassay for Quantitation of Antibodies to Alphaviruses with Staphylococcal Protein A
Jahrling, Peter B., Hesse, Richard A., Metzger, Joseph F.
A radioimmunoassay (RIA) procedure is described for measuring antibodies to alphaviruses in human and other mammalian sera. The test employed protein Abearing Staphylococcus aureus as a solid-phase...
Kulesh, David A., Baker, Robert O., Loveless, Bonnie M., Norwood, David, Zwiers, Susan H., Mucker, Eric, ...
We designed, optimized, and extensively tested several sensitive and specific real-time PCR assays for rapid detection of both smallpox and pan-orthopox virus DNAs. The assays are based on TaqMan...
Jahrling, Peter B., Dendy, Earl, Eddy, Gerald A.
Splenectomy or pretreatment of adult hamsters with cyclophosphamide (Cytoxan) increased the lethality of the TC-83 vaccine strain of Venezuelan encephalitis virus (VEE), inoculated subcutaneously,...
Jahrling, Peter B., Scherer, William F.
A hamster-virulent strain of Venezuelan encephalitis virus (BeAn8, subtype III) and a benign strain (BeAr35645, subtype IV) each yielded 100 clones with virulence properties like those of the...
Growth Curves and Clearance Rates of Virulent and Benign Venezuelan Encephalitis Viruses in Hamsters
Jahrling, Peter B., Scherer, William F.
Four virulent strains of Venezuelan encephalitis virus attained higher concentrations of infectious virus in bloods of adult hamsters than two benign strains when given subcutaneously. One benign...
Rubins, Kathleen H., Hensley, Lisa E., Jahrling, Peter B., Whitney, Adeline R., Geisbert, Thomas W., Huggins, John W., ...
Smallpox has played an unparalleled role in human history and remains a significant potential threat to public health. Despite the historical significance of this disease, we know little about the...
Jahrling, Peter B., Hensley, Lisa E., Martinez, Mark J., LeDuc, James W., Rubins, Kathleen H., Relman, David A., ...
Smallpox virus (variola) poses a significant threat as an agent of bioterrorism. To mitigate this risk, antiviral drugs and an improved vaccine are urgently needed. Satisfactory demonstration of...
Development of a New Vaccine for the Prevention of Lassa Fever
Geisbert, Thomas W, Jones, Steven, Fritz, Elizabeth A, Shurtleff, Amy C, Geisbert, Joan B, Liebscher, Ryan, ...
A replication-competent vaccine based on attenuated recombinant vesicular stomatitis virus vector protects non- human primates.
McCarthy, James P., Bodroghy, Robert S., Jahrling, Peter B., Sobocinski, Philip Z.
Previous studies have shown that stimulation of the oxidative metabolism in polymorphonuclear leukocytes (PMN) by in vitro phagocytosis of various microorganisms results in photon emission, termed...
Cynomolgus Macaque as an Animal Model for Severe Acute Respiratory Syndrome
Lawler, James V, Endy, Timothy P, Hensley, Lisa E, Garrison, Aura, Fritz, Elizabeth A, Lesar, May, ...
Jason Paragas and colleagues report the first findings of measurable clinical disease in nonhuman primates infected with the virus that causes SARS, SARS-CoV.
Sullivan, Nancy J, Geisbert, Thomas W, Geisbert, Joan B, Shedlock, Devon J, Xu, Ling, Lamoreaux, Laurie, ...
A simplified Ebola vaccine that consists of a modified GP protein (which is well-tolerated by human cells even at high concentrations) in a replication-defective adenoviral vector protects macaques.
Ebola Virus Can Be Effectively Neutralized by Antibody Produced in Natural Human Infection
Maruyama, Toshiaki, Rodriguez, Luis L., Jahrling, Peter B., Sanchez, Anthony, Khan, Ali S., Nichol, Stuart T., ...
The activity of antibodies against filoviruses is poorly understood but has important consequences for vaccine design and passive prophylaxis. To investigate this activity, a panel of recombinant...
Geisbert, Tom W., Maruyama, Toshiaki, Jahrling, Peter B., Burton, Dennis R.
A neutralizing human monoclonal antibody, KZ52, protects guinea pigs from lethal Ebola Zaire virus challenge. Administration before or up to 1 h after challenge resulted in dose-dependent protection...
Real-Time PCR Assay To Detect Smallpox Virus
Sofi Ibrahim, M., Kulesh, David A., Saleh, Sharron S., Damon, Inger K., Esposito, Joseph J., Schmaljohn, Alan L., ...
We developed a highly sensitive and specific assay for the rapid detection of smallpox virus DNA on both the Smart Cycler and LightCycler platforms. The assay is based on TaqMan chemistry with the...
Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus
Yount, Boyd, Curtis, Kristopher M., Fritz, Elizabeth A., Hensley, Lisa E., Jahrling, Peter B., Prentice, Erik, ...
A previously undescribed coronavirus (CoV) is the etiologic agent responsible for severe acute respiratory syndrome (SARS). Using a panel of contiguous cDNAs that span the entire genome, we have...
Radioimmunoassay for Quantitation of Antibodies to Alphaviruses with Staphylococcal Protein A
Jahrling, Peter B., Hesse, Richard A., Metzger, Joseph F.
A radioimmunoassay (RIA) procedure is described for measuring antibodies to alphaviruses in human and other mammalian sera. The test employed protein Abearing Staphylococcus aureus as a solid-phase...
Kulesh, David A., Baker, Robert O., Loveless, Bonnie M., Norwood, David, Zwiers, Susan H., Mucker, Eric, ...
We designed, optimized, and extensively tested several sensitive and specific real-time PCR assays for rapid detection of both smallpox and pan-orthopox virus DNAs. The assays are based on TaqMan...
Jahrling, Peter B., Dendy, Earl, Eddy, Gerald A.
Splenectomy or pretreatment of adult hamsters with cyclophosphamide (Cytoxan) increased the lethality of the TC-83 vaccine strain of Venezuelan encephalitis virus (VEE), inoculated subcutaneously,...
Jahrling, Peter B., Scherer, William F.
A hamster-virulent strain of Venezuelan encephalitis virus (BeAn8, subtype III) and a benign strain (BeAr35645, subtype IV) each yielded 100 clones with virulence properties like those of the...
Growth Curves and Clearance Rates of Virulent and Benign Venezuelan Encephalitis Viruses in Hamsters
Jahrling, Peter B., Scherer, William F.
Four virulent strains of Venezuelan encephalitis virus attained higher concentrations of infectious virus in bloods of adult hamsters than two benign strains when given subcutaneously. One benign...
Rubins, Kathleen H., Hensley, Lisa E., Jahrling, Peter B., Whitney, Adeline R., Geisbert, Thomas W., Huggins, John W., ...
Smallpox has played an unparalleled role in human history and remains a significant potential threat to public health. Despite the historical significance of this disease, we know little about the...
Jahrling, Peter B., Hensley, Lisa E., Martinez, Mark J., LeDuc, James W., Rubins, Kathleen H., Relman, David A., ...
Smallpox virus (variola) poses a significant threat as an agent of bioterrorism. To mitigate this risk, antiviral drugs and an improved vaccine are urgently needed. Satisfactory demonstration of...
McCarthy, James P., Bodroghy, Robert S., Jahrling, Peter B., Sobocinski, Philip Z.
Previous studies have shown that stimulation of the oxidative metabolism in polymorphonuclear leukocytes (PMN) by in vitro phagocytosis of various microorganisms results in photon emission, termed...
Development of a New Vaccine for the Prevention of Lassa Fever
Geisbert, Thomas W, Jones, Steven, Fritz, Elizabeth A, Shurtleff, Amy C, Geisbert, Joan B, Liebscher, Ryan, ...
A replication-competent vaccine based on attenuated recombinant vesicular stomatitis virus vector protects non- human primates.
Cynomolgus Macaque as an Animal Model for Severe Acute Respiratory Syndrome
Lawler, James V, Endy, Timothy P, Hensley, Lisa E, Garrison, Aura, Fritz, Elizabeth A, Lesar, May, ...
Jason Paragas and colleagues report the first findings of measurable clinical disease in nonhuman primates infected with the virus that causes SARS, SARS-CoV.