Scientific Commons: Paula M. Pitha

The interferon regulatory factor, IRF5, is a central mediator of toll-like receptor 7 signaling (2005)

Schoenemeyer, Annett, Barnes, Betsy J., Mancl, Margo E., Latz, Eicke, Goutagny, Nadege, Pitha, Paula M., ...

Interferon regulatory factors (IRFs) are critical components of virus-induced immune activation and type I interferon regulation. IRF3 and IRF7 are activated in response to a variety of viruses or...

LPS-TLR4 signaling to IRF-3/7 and NF-kappaB involves the toll adapters TRAM and TRIF (2003)

Fitzgerald, Katherine A., Rowe, Daniel C., Barnes, Betsy J., Caffrey, Daniel R., Visintin, Alberto, Latz, Eicke, ...

Toll-IL-1-resistance (TIR) domain-containing adaptor-inducing IFN-beta (TRIF)-related adaptor molecule (TRAM) is the fourth TIR domain-containing adaptor protein to be described that participates in...

Functional Analysis of Human Herpesvirus 8-Encoded Viral Interferon Regulatory Factor 1 and Its Association with Cellular Interferon Regulatory Factors and p300

Burýšek, Ladislav, Yeow, Wen-Shuz, Lubyová, Barbora, Kellum, Merrill, Schafer, Susan L., Huang, Yao Qi, ...

Human herpesvirus 8/Kaposi sarcoma-associated virus (HHV-8/KSHV) contains, in addition to genes required for viral replication, a unique set of nonstructural genes which may be part of viral mimicry...

Infection with Human Immunodeficiency Virus Type 1 Upregulates DNA Methyltransferase, Resulting in De Novo Methylation of the Gamma Interferon (IFN-γ) Promoter and Subsequent Downregulation of IFN-γ Production

Mikovits, Judy A., Young, Howard A., Vertino, Paula, Issa, Jean-Pierre J., Pitha, Paula M., Turcoski-Corrales, Susan, ...

The immune response to pathogens is regulated by a delicate balance of cytokines. The dysregulation of cytokine gene expression, including interleukin-12, tumor necrosis factor alpha, and gamma...

Binding of Human Immunodeficiency Virus Type 1 to CD4 and CXCR4 Receptors Differentially Regulates Expression of Inflammatory Genes and Activates the MEK/ERK Signaling Pathway

Popik, Waldemar, Hesselgesser, Joseph E., Pitha, Paula M.

We have previously shown that binding of human immunodeficiency virus type 1 (HIV-1) virions to CD4 receptors stimulates association of Lck with Raf-1 and results in the activation of Raf-1 kinase in...

Virus-Dependent Phosphorylation of the IRF-3 Transcription Factor Regulates Nuclear Translocation, Transactivation Potential, and Proteasome-Mediated Degradation

Lin, Rongtuan, Heylbroeck, Christophe, Pitha, Paula M., Hiscott, John

The interferon regulatory factors (IRF) consist of a growing family of related transcription proteins first identified as regulators of the alpha beta interferon (IFN-α/β) gene promoters, as well...

Inhibition of CD3/CD28-Mediated Activation of the MEK/ERK Signaling Pathway Represses Replication of X4 but Not R5 Human Immunodeficiency Virus Type 1 in Peripheral Blood CD4+ T Lymphocytes

Popik, Waldemar, Pitha, Paula M.

Binding of human immunodeficiency virus type 1 (HIV-1) to CD4 receptors induces multiple cellular signaling pathways, including the MEK/ERK cascade. While the interaction of X4 HIV-1 with CXCR4 does...

Characterization of a Novel Human Herpesvirus 8-Encoded Protein, vIRF-3, That Shows Homology to Viral and Cellular Interferon Regulatory Factors

Lubyova, Barbora, Pitha, Paula M.

The genome of the human herpesvirus 8 (HHV-8) contains a cluster of open reading frames (ORFs) encoding proteins with homology to the cellular transcription factors of the interferon regulatory...

Latently Expressed Human Herpesvirus 8-Encoded Interferon Regulatory Factor 2 Inhibits Double-Stranded RNA-Activated Protein Kinase

Buryýšek, Ladislav, Pitha, Paula M.

Human herpesvirus 8 (HHV-8; Kaposi's sarcoma herpesvirus) encodes four open reading frames with homology to cellular proteins of interferon regulatory factor (IRF) family. Three of them, viral IRF-1...

Downregulation of IRF-3 Levels by Ribozyme Modulates the Profile of IFNA Subtypes Expressed in Infected Human Cells

Yeow, Wen-Shuz, Au, Wei-Chun, Lowther, William J., Pitha, Paula M.

As an early response to viral infection, cells express a number of cellular genes that play a role in innate immunity, including alpha/beta interferons (IFN). IFN-α/β are encoded by a single IFNB...

Essential Role of Interferon Regulatory Factor 3 in Direct Activation of RANTES Chemokine Transcription

Lin, Rongtuan, Heylbroeck, Christophe, Genin, Pierre, Pitha, Paula M., Hiscott, John

Localized and systemic cytokine production in virus-infected cells play an important role in the outcome of viral infection and pathogenicity. Activation of the interferon regulatory factors (IRF) in...

Multiple Regulatory Domains of IRF-5 Control Activation, Cellular Localization, and Induction of Chemokines That Mediate Recruitment of T Lymphocytes

Barnes, Betsy J., Kellum, Merrill J., Field, Ann E., Pitha, Paula M.

Transcription factors of the interferon regulatory factor (IRF) family have been identified as critical mediators of early inflammatory gene transcription in infected cells. We recently determined...

Regulation of DNA-Raised Immune Responses by Cotransfected Interferon Regulatory Factors

Sasaki, Shin, Amara, Rama Rao, Yeow, Wen-Shuz, Pitha, Paula M., Robinson, Harriet L.

Interferon regulatory factor 1 (IRF-1), IRF-3, and IRF-7 have been tested as genetic adjuvants for influenza virus hemagglutinin (HA) and nucleoprotein vaccine DNAs. Cotransfection of HA with IRF-3...

Molecular basis for the immunostimulatory activity of guanine nucleoside analogs: Activation of Toll-like receptor 7

Lee, Jongdae, Chuang, Tsung-Hsien, Redecke, Vanessa, She, Liping, Pitha, Paula M., Carson, Dennis A., ...

Certain C8-substituted and N7, C8-disubstituted guanine ribonucleosides comprise a class of small molecules with immunostimulatory activity. In a variety of animal models, these agents stimulate both...

Pitha, Paula M.

Inhibition of Murine Leukemia Virus Replication by Poly(vinyluracil) and Poly(vinyladenine)

Pitha, Paula M., Teich, Natalie M., Lowy, Douglas R., Pitha, Josef

Poly(1-vinyluracil) and poly(9-vinyladenine), as well as the corresponding polynucleotides poly(uridylate) and poly(adenylate), inhibit acute murine leukemia virus infection in mouse-embryo cells,...

Innate antiviral response targets HIV-1 release by the induction of ubiquitin-like protein ISG15

Okumura, Atsushi, Lu, Gengshi, Pitha-Rowe, Ian, Pitha, Paula M.

The goal of this study was to elucidate the molecular mechanism by which type I IFN inhibits assembly and release of HIV-1 virions. Our study revealed that the IFN-induced ubiquitin-like protein...

Rearrangement of Genes Located on Homologous Chromosomal Segments in Mouse and Man: The Location of Genes for Alpha-and Beta-Interferon, Alpha-1 Acid Glycoprotein-1 and -2, and Aminolevulinate Dehydratase on Mouse Chromosome 4

Nadeau, Joseph H., Berger, Franklin G., Kelley, Kevin A., Pitha, Paula M., Sidman, Charles L., Worrall, Neil

Gene mapping studies to determine the order of alpha- and beta-interferon (Ifa, Ifb), aminolevulinate dehydratase (Lv), and alpha-1 acid glycoprotein-1 and -2 ( Orm-1, Orm-2) relative to each other...