Scientific Commons: Paul Britton

DetectiV: visualization, normalization and significance testing for pathogen-detection microarray data (2007)

Watson, Michael, Dukes, Juliet, Abu-Median, Abu-Bakr, King, Donald P, Britton, Paul

Abstract DNA microarrays offer the possibility of testing for the presence of thousands of micro-organisms in a single experiment. However, there is a lack of reliable bioinformatics tools for the...

cis-Acting Sequences Required for Coronavirus Infectious Bronchitis Virus Defective-RNA Replication and Packaging

Dalton, Kevin, Casais, Rosa, Shaw, Kathy, Stirrups, Kathleen, Evans, Sharon, Britton, Paul, ...

The parts of the RNA genome of infectious bronchitis virus (IBV) required for replication and packaging of the RNA were investigated using deletion mutagenesis of a defective RNA (D-RNA) CD-61 (6.1...

The Coronavirus Infectious Bronchitis Virus Nucleoprotein Localizes to the Nucleolus

Hiscox, Julian A., Wurm, Torsten, Wilson, Louise, Britton, Paul, Cavanagh, David, Brooks, Gavin

The coronavirus nucleoprotein (N) has been reported to be involved in various aspects of virus replication. We examined by confocal microscopy the subcellular localization of the avian infectious...

Localization to the Nucleolus Is a Common Feature of Coronavirus Nucleoproteins, and the Protein May Disrupt Host Cell Division

Wurm, Torsten, Chen, Hongying, Hodgson, Teri, Britton, Paul, Brooks, Gavin, Hiscox, Julian A.

The subcellular localization of transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV) (group I and group II coronaviruses, respectively) nucleoproteins (N proteins) were examined...

Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus

Casais, Rosa, Thiel, Volker, Siddell, Stuart G., Cavanagh, David, Britton, Paul

Major advances in the study of the molecular biology of RNA viruses have resulted from the ability to generate and manipulate full-length genomic cDNAs of the viral genomes with the subsequent...

Interaction of the Coronavirus Nucleoprotein with Nucleolar Antigens and the Host Cell

Chen, Hongying, Wurm, Torsten, Britton, Paul, Brooks, Gavin, Hiscox, Julian A.

Coronavirus nucleoproteins (N proteins) localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N...

In Vitro and In Ovo Expression of Chicken Gamma Interferon by a Defective RNA of Avian Coronavirus Infectious Bronchitis Virus

Hackney, Karen, Cavanagh, Dave, Kaiser, Pete, Britton, Paul

Coronavirus defective RNAs (D-RNAs) have been used for site-directed mutagenesis of coronavirus genomes and for expression of heterologous genes. D-RNA CD-61 derived from the avian coronavirus...

Recombinant Avian Infectious Bronchitis Virus Expressing a Heterologous Spike Gene Demonstrates that the Spike Protein Is a Determinant of Cell Tropism

Casais, Rosa, Dove, Brian, Cavanagh, David, Britton, Paul

A recombinant infectious bronchitis virus (IBV), BeauR-M41(S), was generated using our reverse genetics system (R. Casais, V. Thiel, S. G. Siddell, D. Cavanagh, and P. Britton, J. Virol....

Presence of an Encephalomyocarditis Virus Internal Ribosome Entry Site Sequence in Avian Infectious Bronchitis Virus Defective RNAs Abolishes Rescue by Helper Virus

Dove, Brian, Cavanagh, David, Britton, Paul

Avian infectious bronchitis virus (IBV) defective RNAs (D-RNAs) have been used for the expression of heterologous genes in a helper-virus-dependent expression system. The heterologous genes were...

Recombinant Infectious Bronchitis Coronavirus Beaudette with the Spike Protein Gene of the Pathogenic M41 Strain Remains Attenuated but Induces Protective Immunity

Hodgson, Teri, Casais, Rosa, Dove, Brian, Britton, Paul, Cavanagh, Dave

We have replaced the ectodomain of the spike (S) protein of the Beaudette strain (Beau-R; apathogenic for Gallus domesticus chickens) of avian infectious bronchitis coronavirus (IBV) with that from...

Gene 5 of the Avian Coronavirus Infectious Bronchitis Virus Is Not Essential for Replication

Casais, Rosa, Davies, Marc, Cavanagh, David, Britton, Paul

The avian coronavirus Infectious bronchitis virus (IBV), like other coronaviruses, expresses several small nonstructural (ns) proteins in addition to those from gene 1 (replicase) and the structural...

Neither the RNA nor the Proteins of Open Reading Frames 3a and 3b of the Coronavirus Infectious Bronchitis Virus Are Essential for Replication

Hodgson, Teri, Britton, Paul, Cavanagh, Dave

Gene 3 of infectious bronchitis virus is tricistronic; open reading frames (ORFs) 3a and 3b encode two small nonstructural (ns) proteins, 3a and 3b, of unknown function, and a third, structural...