Paul A. Lefebvre

Molecular Map of the Chlamydomonas reinhardtii Nuclear Genome

Kathir, Pushpa, LaVoie, Matthew, Brazelton, William J., Haas, Nancy A., Lefebvre, Paul A., Silflow, Carolyn D.

We have prepared a molecular map of the Chlamydomonas reinhardtii genome anchored to the genetic map. The map consists of 264 markers, including sequence-tagged sites (STS), scored by use of PCR and...

PF15p Is the Chlamydomonas Homologue of the Katanin p80 Subunit and Is Required for Assembly of Flagellar Central Microtubules

Dymek, Erin E., Lefebvre, Paul A., Smith, Elizabeth F.

Numerous studies have indicated that the central apparatus plays a significant role in regulating flagellar motility, yet little is known about how the central pair of microtubules or their...

Regulation of Flagellar Assembly by Glycogen Synthase Kinase 3 in Chlamydomonas reinhardtii

Wilson, Nedra F., Lefebvre, Paul A.

Chlamydomonas reinhardtii controls flagellar assembly such that flagella are of an equal and predetermined length. Previous studies demonstrated that lithium, an inhibitor of glycogen synthase kinase...

The LF1 Gene of Chlamydomonas reinhardtii Encodes a Novel Protein Required for Flagellar Length Control

Nguyen, Rachel L., Tam, Lai-Wa, Lefebvre, Paul A.

Flagellar length is tightly regulated in the biflagellate alga Chlamydomonas reinhardtii. Several genes required for control of flagellar length have been identified, including LF1, a gene required...

Molecular Map of the Chlamydomonas reinhardtii Nuclear Genome

Kathir, Pushpa, LaVoie, Matthew, Brazelton, William J., Haas, Nancy A., Lefebvre, Paul A., Silflow, Carolyn D.

We have prepared a molecular map of the Chlamydomonas reinhardtii genome anchored to the genetic map. The map consists of 264 markers, including sequence-tagged sites (STS), scored by use of PCR and...

PF15p Is the Chlamydomonas Homologue of the Katanin p80 Subunit and Is Required for Assembly of Flagellar Central Microtubules

Dymek, Erin E., Lefebvre, Paul A., Smith, Elizabeth F.

Numerous studies have indicated that the central apparatus plays a significant role in regulating flagellar motility, yet little is known about how the central pair of microtubules or their...

Regulation of Flagellar Assembly by Glycogen Synthase Kinase 3 in Chlamydomonas reinhardtii

Wilson, Nedra F., Lefebvre, Paul A.

Chlamydomonas reinhardtii controls flagellar assembly such that flagella are of an equal and predetermined length. Previous studies demonstrated that lithium, an inhibitor of glycogen synthase kinase...

The LF1 Gene of Chlamydomonas reinhardtii Encodes a Novel Protein Required for Flagellar Length Control

Nguyen, Rachel L., Tam, Lai-Wa, Lefebvre, Paul A.

Flagellar length is tightly regulated in the biflagellate alga Chlamydomonas reinhardtii. Several genes required for control of flagellar length have been identified, including LF1, a gene required...

A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas

Tam, Lai-Wa, Wilson, Nedra F., Lefebvre, Paul A.

Little is known about how cells regulate the size of their organelles. In this study, we find that proper flagellar length control in Chlamydomonas reinhardtii requires the activity of a new member...

The Uni2 Phosphoprotein is a Cell Cycle–regulated Component of the Basal Body Maturation Pathway in Chlamydomonas reinhardtii

Piasecki, Brian P., LaVoie, Matthew, Tam, Lai-Wa, Lefebvre, Paul A., Silflow, Carolyn D.

Mutations in the UNI2 locus in Chlamydomonas reinhardtii result in a “uniflagellar” phenotype in which flagellar assembly occurs preferentially from the older basal body and ultrastructural...

Bld10p, a novel protein essential for basal body assembly in Chlamydomonas: localization to the cartwheel, the first ninefold symmetrical structure appearing during assembly

Matsuura, Kumi, Lefebvre, Paul A., Kamiya, Ritsu, Hirono, Masafumi

How centrioles and basal bodies assemble is a long-standing puzzle in cell biology. To address this problem, we analyzed a novel basal body-defective Chlamydomonas reinhardtii mutant isolated from a...

Defective flagellar assembly and length regulation in LF3 null mutants in Chlamydomonas

Tam, Lai-Wa, Dentler, William L., Lefebvre, Paul A.

Four long-flagella (LF) genes are important for flagellar length control in Chlamydomonas reinhardtii. Here, we characterize two new null lf3 mutants whose phenotypes are different from previously...

The Vfl1 Protein in Chlamydomonas Localizes in a Rotationally Asymmetric Pattern at the Distal Ends of the Basal Bodies

Silflow, Carolyn D., LaVoie, Matthew, Tam, Lai-Wa, Tousey, Susan, Sanders, Mark, Wu, Wei-chien, ...

In the unicellular alga Chlamydomonas, two anterior flagella are positioned with 180° rotational symmetry, such that the flagella beat with the effective strokes in opposite directions (Hoops, H.J.,...

Nitrate Signaling by the Regulatory Gene NIT2 in Chlamydomonas[W]

Camargo, Antonio, Llamas, Ángel, Schnell, Rogene A., Higuera, José J., González-Ballester, David, Lefebvre, Paul A., ...

Positive signaling by nitrate in its assimilation pathway has been studied in Chlamydomonas reinhardtii. Among >34,000 lines generated by plasmid insertion, 10 mutants were unable to activate nitrate...