Yee, Janet, Tang, Anita, Lau, Wei-Ling, Ritter, Heather, Delport, Dewald, Page, Melissa, ...
Abstract Background Giardia intestinalis is a protist found in freshwaters worldwide, and is the most common cause of parasitic diarrhea in humans. The phylogenetic position of this parasite is still...
Benno H. Ter Kuile, Ivan Hrdý, Lidya B. Sánchez, Miklós Müller
Two isoforms of α-glucosidase were purified from the parasitic protist Trichomonas vaginalis. Both consisted of 103 kDa subunits, but differed in pH optimum and substrate specificity. Isoform 1 had...
Hashimoto, Tetsuo, Sánchez, Lidya B., Shirakura, Tetsurou, Müller, Miklós, Hasegawa, Masami
Nuclear-coded valyl-tRNA synthetase (ValRS) of eukaryotes is regarded of mitochondrial origin. Complete ValRS sequences obtained by us from two amitochondriate protists, the diplomonad, Giardia...
Convergent evolution of Trichomonas vaginalis lactate dehydrogenase from malate dehydrogenase
Wu, Gang, Fiser, András, Ter Kuile, Benno, Šali, Andrej, Müller, Miklós
Lactate dehydrogenase (LDH) is present in the amitochondriate parasitic protist Trichomonas vaginalis and some but not all other trichomonad species. The derived amino acid sequence of T. vaginalis...
Müller, Miklós, Lee, Jennifer A., Gordon, Paul, Gaasterland, Terry, Sensen, Christoph W.
Inorganic pyrophosphate-dependent phosphofructokinase (PPi-PFK) of the amitochondriate eukaryote Mastigamoeba balamuthi was sequenced and showed about 60% identity to PPi-PFKs from two eubacteria,...
Bapteste, Eric, Brinkmann, Henner, Lee, Jennifer A., Moore, Dorothy V., Sensen, Christoph W., Gordon, Paul, ...
The phylogenetic relationships of amoebae are poorly resolved. To address this difficult question, we have sequenced 1,280 expressed sequence tags from Mastigamoeba balamuthi and assembled a large...
Assay Conditions and the Demonstration of Nitroimidazole Resistance in Tritrichomonas foetus
Meingassner, Josef G., Mieth, Hubert, Czok, Rudolf, Lindmark, Donald G., Müller, Miklós
Tritrichomonas foetus KV1, a nitroimidazole-susceptible strain, and KV1/M100, its nitroimidazole-resistant daughter strain, differed markedly in their in vivo susceptibility to metronidazole. In...
Absence of Strand Breaks in Deoxyribonucleic Acid Treated with Metronidazole
LaRusso, Nicholas F., Tomasz, Maria, Kaplan, David, Müller, Miklós
The deoxyribonucleic acid (DNA)-degrading potential of metronidazole was evaluated in vitro by three techniques: determination of melting curve, measurement of viscosity, and centrifugation in...
Antitrichomonad Action, Mutagenicity, and Reduction of Metronidazole and Other Nitroimidazoles
Lindmark, Donald G., Müller, Miklós
Twelve 4- and 5-nitroimidazole derivatives, including metronidazole and two of its metabolites, tinidazole, dimetridazole, and nimorazole, were tested for antitrichomonad action on Tritrichomonas...
Sutak, Robert, Dolezal, Pavel, Fiumera, Heather L., Hrdy, Ivan, Dancis, Andrew, Delgadillo-Correa, Maria, ...
Mitochondria are the site of assembly of FeS centers of mitochondrial and cytosolic FeS proteins. Various microaerophilic or anaerobic unicellular eukaryotes lack typical mitochondria...
Hashimoto, Tetsuo, Sánchez, Lidya B., Shirakura, Tetsurou, Müller, Miklós, Hasegawa, Masami
Nuclear-coded valyl-tRNA synthetase (ValRS) of eukaryotes is regarded of mitochondrial origin. Complete ValRS sequences obtained by us from two amitochondriate protists, the diplomonad, Giardia...
Convergent evolution of Trichomonas vaginalis lactate dehydrogenase from malate dehydrogenase
Wu, Gang, Fiser, András, Ter Kuile, Benno, Šali, Andrej, Müller, Miklós
Lactate dehydrogenase (LDH) is present in the amitochondriate parasitic protist Trichomonas vaginalis and some but not all other trichomonad species. The derived amino acid sequence of T. vaginalis...
Müller, Miklós, Lee, Jennifer A., Gordon, Paul, Gaasterland, Terry, Sensen, Christoph W.
Inorganic pyrophosphate-dependent phosphofructokinase (PPi-PFK) of the amitochondriate eukaryote Mastigamoeba balamuthi was sequenced and showed about 60% identity to PPi-PFKs from two eubacteria,...
Bapteste, Eric, Brinkmann, Henner, Lee, Jennifer A., Moore, Dorothy V., Sensen, Christoph W., Gordon, Paul, ...
The phylogenetic relationships of amoebae are poorly resolved. To address this difficult question, we have sequenced 1,280 expressed sequence tags from Mastigamoeba balamuthi and assembled a large...
Assay Conditions and the Demonstration of Nitroimidazole Resistance in Tritrichomonas foetus
Meingassner, Josef G., Mieth, Hubert, Czok, Rudolf, Lindmark, Donald G., Müller, Miklós
Tritrichomonas foetus KV1, a nitroimidazole-susceptible strain, and KV1/M100, its nitroimidazole-resistant daughter strain, differed markedly in their in vivo susceptibility to metronidazole. In...
Absence of Strand Breaks in Deoxyribonucleic Acid Treated with Metronidazole
LaRusso, Nicholas F., Tomasz, Maria, Kaplan, David, Müller, Miklós
The deoxyribonucleic acid (DNA)-degrading potential of metronidazole was evaluated in vitro by three techniques: determination of melting curve, measurement of viscosity, and centrifugation in...
Antitrichomonad Action, Mutagenicity, and Reduction of Metronidazole and Other Nitroimidazoles
Lindmark, Donald G., Müller, Miklós
Twelve 4- and 5-nitroimidazole derivatives, including metronidazole and two of its metabolites, tinidazole, dimetridazole, and nimorazole, were tested for antitrichomonad action on Tritrichomonas...
Sutak, Robert, Dolezal, Pavel, Fiumera, Heather L., Hrdy, Ivan, Dancis, Andrew, Delgadillo-Correa, Maria, ...
Mitochondria are the site of assembly of FeS centers of mitochondrial and cytosolic FeS proteins. Various microaerophilic or anaerobic unicellular eukaryotes lack typical mitochondria...
Core histone genes of Giardia intestinalis: genomic organization, promoter structure, and expression
Yee, Janet, Tang, Anita, Lau, Wei-Ling, Ritter, Heather, Delport, Dewald, Page, Melissa, ...
SECRETION OF ACID HYDROLASES AND ITS INTRACELLULAR SOURCE IN TETRAHYMENA PYRIFORMIS
Axenic Tetrahymena pyriformis, syngen 1, mating type II cells were grown in Cox's defined medium. When washed and transferred into nonnutrient dilute salt solution or resuspended in the defined...
To determine the localization of several enzymes in Tritrichomonas foetus, the axenic KV-1 strain was grown in Diamond's medium with bovine serum, homogenized in 0.25 M sucrose, and subjected to...