Lundin, Victor F., Srayko, Martin, Hyman, Anthony A., Leroux, Michel R.
The efficient folding of actin and tubulin in vitro and in Saccharomyces cerevisiae is known to require the molecular chaperones prefoldin and CCT, yet little is known about the functions of these...
Stirling, Peter C., Srayko, Martin, Takhar, Karam S., Pozniakovsky, Andrei, Hyman, Anthony A., Leroux, Michel R.
The Chaperonin Containing Tcp1 (CCT) maintains cellular protein folding homeostasis in the eukaryotic cytosol by assisting the biogenesis of many proteins, including actins, tubulins, and regulators...
Chen, Nansheng, Mah, Allan, Blacque, Oliver E, Chu, Jeffrey, Phgora, Kiran, Bakhoum, Mathieu W, ...
Abstract Background The recent availability of genome sequences of multiple related Caenorhabditis species has made it possible to identify, using comparative genomics, similarly transcribed genes in...
Fändrich, Marcus, Tito, Mark A., Leroux, Michel R., Rostom, Adam A., Hartl, F. Ulrich, Dobson, Christopher M., ...
We have analyzed a newly described archaeal GimC/prefoldin homologue, termed MtGimC, by using nanoflow electrospray coupled with time-of-flight MS. The molecular weight of the complex from...
Lundin, Victor F., Stirling, Peter C., Gomez-Reino, Juan, Mwenifumbo, Jill C., Obst, Jennifer M., Valpuesta, José M., ...
Prefoldin (PFD) is a jellyfish-shaped molecular chaperone that has been proposed to play a general role in de novo protein folding in archaea and is known to assist the biogenesis of actins,...
Blacque, Oliver E., Reardon, Michael J., Li, Chunmei, McCarthy, Jonathan, Mahjoub, Moe R., Ansley, Stephen J., ...
Bardet-Biedl syndrome (BBS) is a genetically heterogeneous developmental disorder whose molecular basis is largely unknown. Here, we show that mutations in the Caenorhabditis elegans bbs-7 and bbs-8...
Getting a grip on non-native proteins
Stirling, Peter C., Lundin, Victor F., Leroux, Michel R.
It is an underappreciated fact that non-native polypeptides are prevalent in the cellular environment. Native proteins have the folded structure, assembled state and cellular localization required...
Fändrich, Marcus, Tito, Mark A., Leroux, Michel R., Rostom, Adam A., Hartl, F. Ulrich, Dobson, Christopher M., ...
We have analyzed a newly described archaeal GimC/prefoldin homologue, termed MtGimC, by using nanoflow electrospray coupled with time-of-flight MS. The molecular weight of the complex from...
Lundin, Victor F., Stirling, Peter C., Gomez-Reino, Juan, Mwenifumbo, Jill C., Obst, Jennifer M., Valpuesta, José M., ...
Prefoldin (PFD) is a jellyfish-shaped molecular chaperone that has been proposed to play a general role in de novo protein folding in archaea and is known to assist the biogenesis of actins,...
Blacque, Oliver E., Reardon, Michael J., Li, Chunmei, McCarthy, Jonathan, Mahjoub, Moe R., Ansley, Stephen J., ...
Bardet-Biedl syndrome (BBS) is a genetically heterogeneous developmental disorder whose molecular basis is largely unknown. Here, we show that mutations in the Caenorhabditis elegans bbs-7 and bbs-8...
Getting a grip on non-native proteins
Stirling, Peter C., Lundin, Victor F., Leroux, Michel R.
It is an underappreciated fact that non-native polypeptides are prevalent in the cellular environment. Native proteins have the folded structure, assembled state and cellular localization required...
Efimenko, Evgeni, Blacque, Oliver E., Ou, Guangshuo, Haycraft, Courtney J., Yoder, Bradley K., Scholey, Jonathan M., ...
The intraflagellar transport (IFT) machinery required to build functional cilia consists of a multisubunit complex whose molecular composition, organization, and function are poorly understood. Here,...
The WD Repeat-containing Protein IFTA-1 Is Required for Retrograde Intraflagellar Transport
Blacque, Oliver E., Li, Chunmei, Inglis, Peter N., Esmail, Muneer A., Ou, Guangshuo, Mah, Allan K., ...
The assembly and maintenance of cilia require intraflagellar transport (IFT), a microtubule-dependent bidirectional motility of multisubunit protein complexes along ciliary axonemes. Defects in IFT...
Chen, Nansheng, Mah, Allan, Blacque, Oliver E, Chu, Jeffrey, Phgora, Kiran, Bakhoum, Mathieu W, ...
Comparative genomic analysis of three nematode species identifies 93 genes that encode putative components of the ciliated neurons in C. elegans and are subject to the same regulatory control.
Ou, Guangshuo, Koga, Makato, Blacque, Oliver E., Murayama, Takashi, Ohshima, Yasumi, Schafer, Jenny C., ...
Sensory cilium biogenesis within Caenorhabditis elegans neurons depends on the kinesin-2–dependent intraflagellar transport (IFT) of ciliary precursors associated with IFT particles to the axoneme...
Stirling, Peter C., Srayko, Martin, Takhar, Karam S., Pozniakovsky, Andrei, Hyman, Anthony A., Leroux, Michel R.
The C haperonin Containing Tcp1 (CCT) maintains cellular protein folding homeostasis in the eukaryotic cytosol by assisting the biogenesis of many proteins, including actins, tubulins, and regulators...
Pan, Xiaoyu, Ou, Guangshuo, Civelekoglu-Scholey, Gul, Blacque, Oliver E., Endres, Nicholas F., Tao, Li, ...
The assembly and function of cilia on Caenorhabditis elegans neurons depends on the action of two kinesin-2 motors, heterotrimeric kinesin-II and homodimeric OSM-3–kinesin, which cooperate to move...
An Essential Role for DYF-11/MIP-T3 in Assembling Functional Intraflagellar Transport Complexes
Li, Chunmei, Inglis, Peter N., Leitch, Carmen C., Efimenko, Evgeni, Zaghloul, Norann A., Mok, Calvin A., ...
MIP-T3 is a human protein found previously to associate with microtubules and the kinesin-interacting neuronal protein DISC1 (Disrupted-in-Schizophrenia 1), but whose cellular function(s) remains...
Loss of Bardet–Biedl syndrome proteins causes defects in peripheral sensory innervation and function
Tan, Perciliz L., Barr, Travis, Inglis, Peter N., Mitsuma, Norimasa, Huang, Susan M., Garcia-Gonzalez, Miguel A., ...
Reception and interpretation of environmental stimuli is critical for the survival of all organisms. Here, we show that the ablation of BBS1 and BBS4, two genes mutated in Bardet–Biedl syndrome and...