Michael L. Whitfield

Universal Reference RNA as a standard for microarray experiments (2004)

Novoradovskaya, Natalia, Whitfield, Michael L, Basehore, Lee S, Novoradovsky, Alexey, Pesich, Robert, Usary, Jerry, ...

Abstract Background Obtaining reliable and reproducible two-color microarray gene expression data is critically important for understanding the biological significance of perturbations made on a...

Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis (2002)

Zhao, Hongjuan, Hastie, Trevor, Whitfield, Michael L, Børresen-Dale, Anne-Lise, Jeffrey, Stefanie S

Abstract Background T7 based linear amplification of RNA is used to obtain sufficient antisense RNA for microarray expression profiling. We optimized and systematically evaluated the fidelity and...

Stem-Loop Binding Protein, the Protein That Binds the 3′ End of Histone mRNA, Is Cell Cycle Regulated by Both Translational and Posttranslational Mechanisms

Whitfield, Michael L., Zheng, Lian-Xing, Baldwin, Amy, Ohta, Tomohiko, Hurt, Myra M., Marzluff, William F.

The expression of the replication-dependent histone mRNAs is tightly regulated during the cell cycle. As cells progress from G1 to S phase, histone mRNA levels increase 35-fold, and they decrease...

Identification of Genes Periodically Expressed in the Human Cell Cycle and Their Expression in TumorsD⃞

Whitfield, Michael L., Sherlock, Gavin, Saldanha, Alok J., Murray, John I., Ball, Catherine A., Alexander, Karen E., ...

The genome-wide program of gene expression during the cell division cycle in a human cancer cell line (HeLa) was characterized using cDNA microarrays. Transcripts of >850 genes showed periodic...

Systemic and cell type-specific gene expression patterns in scleroderma skin

Whitfield, Michael L., Finlay, Deborah R., Murray, John Isaac, Troyanskaya, Olga G., Chi, Jen-Tsan, Pergamenschikov, Alexander, ...

We used DNA microarrays representing >12,000 human genes to characterize gene expression patterns in skin biopsies from individuals with a diagnosis of systemic sclerosis with diffuse scleroderma. We...

Diverse Effects of Methylseleninic Acid on the Transcriptional Program of Human Prostate Cancer Cells

Zhao, Hongjuan, Whitfield, Michael L., Xu, Tong, Botstein, David, Brooks, James D.

Methylseleninic acid (MSA) has been shown to have potent anticancer activity and is an excellent compound for studying the anticancer effects of selenium in vitro. To gain insights into the effects...

Diverse and Specific Gene Expression Responses to Stresses in Cultured Human CellsD⃞

Murray, John Isaac, Whitfield, Michael L., Trinklein, Nathan D., Myers, Richard M., Brown, Patrick O., Botstein, David

We used cDNA microarrays in a systematic study of the gene expression responses of HeLa cells and primary human lung fibroblasts to heat shock, endoplasmic reticulum stress, oxidative stress, and...

SLBP is associated with histone mRNA on polyribosomes as a component of the histone mRNP

Whitfield, Michael L., Kaygun, Handan, Erkmann, Judith A., Dominski, Zbigniew, Marzluff, William F.

The stem–loop binding protein (SLBP) binds the 3′ end of histone mRNA and is present both in nucleus, and in the cytoplasm on the polyribosomes. SLBP participates in the processing of the histone...

A Conserved Myc Protein Domain, MBIV, Regulates DNA Binding, Apoptosis, Transformation, and G2 Arrest†

Cowling, Victoria H., Chandriani, Sanjay, Whitfield, Michael L., Cole, Michael D.

The myc family of oncogenes is well conserved throughout evolution. Here we present the characterization of a domain conserved in c-, N-, and L-Myc from fish to humans, N-Myc317-337, designated Myc...

Stem-Loop Binding Protein, the Protein That Binds the 3′ End of Histone mRNA, Is Cell Cycle Regulated by Both Translational and Posttranslational Mechanisms

Whitfield, Michael L., Zheng, Lian-Xing, Baldwin, Amy, Ohta, Tomohiko, Hurt, Myra M., Marzluff, William F.

The expression of the replication-dependent histone mRNAs is tightly regulated during the cell cycle. As cells progress from G1 to S phase, histone mRNA levels increase 35-fold, and they decrease...

Identification of Genes Periodically Expressed in the Human Cell Cycle and Their Expression in TumorsD⃞

Whitfield, Michael L., Sherlock, Gavin, Saldanha, Alok J., Murray, John I., Ball, Catherine A., Alexander, Karen E., ...

The genome-wide program of gene expression during the cell division cycle in a human cancer cell line (HeLa) was characterized using cDNA microarrays. Transcripts of >850 genes showed periodic...

Systemic and cell type-specific gene expression patterns in scleroderma skin

Whitfield, Michael L., Finlay, Deborah R., Murray, John Isaac, Troyanskaya, Olga G., Chi, Jen-Tsan, Pergamenschikov, Alexander, ...

We used DNA microarrays representing >12,000 human genes to characterize gene expression patterns in skin biopsies from individuals with a diagnosis of systemic sclerosis with diffuse scleroderma. We...

Diverse Effects of Methylseleninic Acid on the Transcriptional Program of Human Prostate Cancer Cells

Zhao, Hongjuan, Whitfield, Michael L., Xu, Tong, Botstein, David, Brooks, James D.

Methylseleninic acid (MSA) has been shown to have potent anticancer activity and is an excellent compound for studying the anticancer effects of selenium in vitro. To gain insights into the effects...

Diverse and Specific Gene Expression Responses to Stresses in Cultured Human CellsD⃞

Murray, John Isaac, Whitfield, Michael L., Trinklein, Nathan D., Myers, Richard M., Brown, Patrick O., Botstein, David

We used cDNA microarrays in a systematic study of the gene expression responses of HeLa cells and primary human lung fibroblasts to heat shock, endoplasmic reticulum stress, oxidative stress, and...

SLBP is associated with histone mRNA on polyribosomes as a component of the histone mRNP

Whitfield, Michael L., Kaygun, Handan, Erkmann, Judith A., Dominski, Zbigniew, Marzluff, William F.

The stem–loop binding protein (SLBP) binds the 3′ end of histone mRNA and is present both in nucleus, and in the cytoplasm on the polyribosomes. SLBP participates in the processing of the histone...

A Conserved Myc Protein Domain, MBIV, Regulates DNA Binding, Apoptosis, Transformation, and G2 Arrest†

Cowling, Victoria H., Chandriani, Sanjay, Whitfield, Michael L., Cole, Michael D.

The myc family of oncogenes is well conserved throughout evolution. Here we present the characterization of a domain conserved in c-, N-, and L-Myc from fish to humans, N-Myc317-337, designated Myc...

Genome-wide analysis of mRNAs bound to the histone stem–loop binding protein

Pendergrass, Sarah A., Marzluff, William F., Whitfield, Michael L.

The replication-dependent histone mRNAs are cell-cycle-regulated and expressed only during S phase. In contrast to all other eukaryotic mRNAs, the histone mRNAs end in a highly conserved...