Analysis of cell-based RNAi screens (2006)
Boutros, Michael, Brás, Lígia P, Huber, Wolfgang
Abstract RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical...
Minimizing the risk of reporting false positives in large-scale RNAi screens (2006)
Echeverri, Christophe J, Beachy, Philip A, Baum, Buzz, Boutros, Michael, Buchholz, Frank, Chanda, Sumit K, ...
Large-scale RNA interference (RNAi)-based analyses, very much as other 'omic' approaches, have inherent rates of false positives and negatives. The variability in the standards of care applied to...
Choe, Sung E, Boutros, Michael, Michelson, Alan M, Church, George M, Halfon, Marc S
Abstract Background As more methods are developed to analyze RNA-profiling data, assessing their performance using control datasets becomes increasingly important. Results We present a 'spike-in'...
Genome-wide RNAi analysis of growth and viability in Drosophila cells (2004)
Boutros,Michael, Kiger,Amy A., Armknecht,Susan, Kerr,Kim, Hild,Marc, Koch,Britta, ...
A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded...
Genome-wide RNAi analysis of growth and viability in Drosophila cells (2004)
Boutros, Michael, Kiger, Amy A., Armknecht, Susan, Kerr, Kim, Hild, Marc, Koch, Britta, ...
A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded...
Wilson, Angus C., Boutros, Michael, Johnson, Kristina M., Herr, Winship
When herpes simplex virus infects permissive cells, the viral regulatory protein VP16 forms a specific complex with HCF-1, a preexisting nuclear protein involved in cell proliferation. The majority...
E-RNAi: a web application to design optimized RNAi constructs
Arziman, Zeynep, Horn, Thomas, Boutros, Michael
RNA interference (RNAi) has become a powerful genetic approach to systematically dissect gene function on a genome-wide scale. Owing to the penetrance and efficiency of RNAi in invertebrates, model...
FlyRNAi: the Drosophila RNAi screening center database
Flockhart, Ian, Booker, Matthew, Kiger, Amy, Boutros, Michael, Armknecht, Susan, Ramadan, Nadire, ...
RNA interference (RNAi) has become a powerful tool for genetic screening in Drosophila. At the Drosophila RNAi Screening Center (DRSC), we are using a library of over 21 000 double-stranded RNAs...
Preferred analysis methods for Affymetrix GeneChips revealed by a wholly defined control dataset
Choe, Sung E, Boutros, Michael, Michelson, Alan M, Church, George M, Halfon, Marc S
A 'spike-in' experiment for Affymetrix GeneChips is described that provides a defined dataset of 3,860 RNA species. A 'best route' combination of analysis methods is presented which allows detection...
Gesellchen, Viola, Kuttenkeuler, David, Steckel, Michael, Pelte, Nadège, Boutros, Michael
Innate immunity in vertebrates and invertebrates is of central importance as a biological programme for host defence against pathogenic challenges. To find novel components of the Drosophila immune...
Wilson, Angus C., Boutros, Michael, Johnson, Kristina M., Herr, Winship
When herpes simplex virus infects permissive cells, the viral regulatory protein VP16 forms a specific complex with HCF-1, a preexisting nuclear protein involved in cell proliferation. The majority...
Preferred analysis methods for Affymetrix GeneChips revealed by a wholly defined control dataset
Choe, Sung E, Boutros, Michael, Michelson, Alan M, Church, George M, Halfon, Marc S
A 'spike-in' experiment for Affymetrix GeneChips is described that provides a defined dataset of 3,860 RNA species. A 'best route' combination of analysis methods is presented which allows detection...
E-RNAi: a web application to design optimized RNAi constructs
Arziman, Zeynep, Horn, Thomas, Boutros, Michael
RNA interference (RNAi) has become a powerful genetic approach to systematically dissect gene function on a genome-wide scale. Owing to the penetrance and efficiency of RNAi in invertebrates, model...
FlyRNAi: the Drosophila RNAi screening center database
Flockhart, Ian, Booker, Matthew, Kiger, Amy, Boutros, Michael, Armknecht, Susan, Ramadan, Nadire, ...
RNA interference (RNAi) has become a powerful tool for genetic screening in Drosophila. At the Drosophila RNAi Screening Center (DRSC), we are using a library of over 21 000 double-stranded RNAs...
Gesellchen, Viola, Kuttenkeuler, David, Steckel, Michael, Pelte, Nadège, Boutros, Michael
Innate immunity in vertebrates and invertebrates is of central importance as a biological programme for host defence against pathogenic challenges. To find novel components of the Drosophila immune...
Analysis of cell-based RNAi screens
Boutros, Michael, Brás, Lígia P, Huber, Wolfgang
cellHTS is a new method for the analysis and documentation of RNAi screens.
GenomeRNAi: a database for cell-based RNAi phenotypes
Horn, Thomas, Arziman, Zeynep, Berger, Juerg, Boutros, Michael
RNA interference (RNAi) has emerged as a powerful tool to generate loss-of-function phenotypes in a variety of organisms. Combined with the sequence information of almost completely annotated...
Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing
Eulalio, Ana, Rehwinkel, Jan, Stricker, Mona, Huntzinger, Eric, Yang, Schu-Fee, Doerks, Tobias, ...
microRNAs (miRNAs) silence gene expression by suppressing protein production and/or by promoting mRNA decay. To elucidate how silencing is accomplished, we screened an RNA interference library for...