ChIP on SNP-chip for genome-wide analysis of human histone H4 hyperacetylation (2007)
McCann, Jennifer A, Muro, Enrique M, Palmer, Claire, Palidwor, Gareth, Porter, Christopher J, Andrade-Navarro, Miguel A, ...
Abstract Background SNP microarrays are designed to genotype Single Nucleotide Polymorphisms (SNPs). These microarrays report hybridization of DNA fragments and therefore can be used for the purpose...
Gene function in early mouse embryonic stem cell differentiation (2007)
Sene, Kagnew, Porter, Christopher J, Palidwor, Gareth, Perez-Iratxeta, Carolina, Muro, Enrique M, Campbell, Pearl A, ...
Abstract Background Little is known about the genes that drive embryonic stem cell differentiation. However, such knowledge is necessary if we are to exploit the therapeutic potential of stem cells....
Gene function in early mouse embryonic stem cell differentiation (2007)
Sene, Kagnew, Porter, Christopher J, Palidwor, Gareth, Perez-Iratxeta, Carolina, Muro, Enrique M, Campbell, Pearl A, ...
Abstract Background Little is known about the genes that drive embryonic stem cell differentiation. However, such knowledge is necessary if we are to exploit the therapeutic potential of stem cells....
Patrick Seale, Jeff Ishibashi, Anthony Scimè, Michael A. Rudnicki
The gene Pax7 is shown to be a key regulator of muscle cell differentiation in specific populations of adult stem cells during muscle tissue regeneration.
Patrick Seale, Jeff Ishibashi, Anthony Scimè, Michael A. Rudnicki
CD45+:Sca1+ adult stem cells isolated from uninjured muscle do not display any myogenic potential, whereas those isolated from regenerating muscle give rise to myoblasts expressing the paired-box...
Severe cardiomyopathy in mice lacking dystrophin and MyoD
Megeney, Lynn A., Kablar, Boris, Perry, Robert L. S., Ying, Chuyan, May, Linda, Rudnicki, Michael A.
The mdx mouse, a mouse model of Duchenne muscular dystrophy, carries a loss-of-function mutation in dystrophin, a component of the membrane-associated dystrophin–glycoprotein complex. Unlike...
Amplification of the neu/erbB-2 oncogene in a mouse model of mammary tumorigenesis
Andrechek, Eran R., Hardy, William R., Siegel, Peter M., Rudnicki, Michael A., Cardiff, Robert D., Muller, William J.
The neu (c-erbB-2, Her-2) protooncogene is amplified and overexpressed in 20–30% of human breast cancers. Although transgenic mouse models have illustrated the role of Neu in the induction of...
Sabourin, Luc A., Seale, Patrick, Wagner, Julian, Rudnicki, Michael A.
We have demonstrated that a novel Ste20-related kinase, designated SLK, mediates apoptosis and actin stress fiber dissolution through distinct domains generated by caspase 3 cleavage. Overexpression...
Male Sexual Dysfunction in Mice Bearing Targeted Mutant Alleles of the PEA3 ets Gene
Laing, Michael A., Coonrod, Scott, Hinton, Barry T., Downie, John W., Tozer, Richard, Rudnicki, Michael A., ...
PEA3, a member of the Ets family of transcriptional regulatory proteins, is expressed in a unique spatial and temporal pattern during mouse embryogenesis; its overexpression is positively correlated...
LeCouter, Jennifer E., Kablar, Boris, Hardy, W. Rodney, Ying, Chuyan, Megeney, Lynn A., May, Linda L., ...
To investigate the function of the Rb-related p107 gene, a null mutation in p107 was introduced into the germ line of mice and bred into a BALB/cJ genetic background. Mice lacking p107 were viable...
ErbB2 Is Required for Muscle Spindle and Myoblast Cell Survival
Andrechek, Eran R., Hardy, William R., Girgis-Gabardo, Adele A., Perry, Robert L. S., Butler, Richard, Graham, Frank L., ...
Signaling mediated by ErbB2 is thought to play a critical role in numerous developmental processes. However, due to the embryonic lethality associated with the germ line inactivation of erbB2, its...
Seale, Patrick, Ishibashi, Jeff, Scimè, Anthony, Rudnicki, Michael A
CD45+:Sca1+ adult stem cells isolated from uninjured muscle do not display any myogenic potential, whereas those isolated from regenerating muscle give rise to myoblasts expressing the paired-box...
Pocket Protein Complexes Are Recruited to Distinct Targets in Quiescent and Proliferating Cells
Balciunaite, Egle, Spektor, Alexander, Lents, Nathan H., Cam, Hugh, Te Riele, Hein, Scime, Anthony, ...
Biochemical and genetic studies have determined that retinoblastoma protein (pRB) tumor suppressor family members have overlapping functions. However, these studies have largely failed to distinguish...
MyoD Synergizes with the E-Protein HEBβ To Induce Myogenic Differentiation
Parker, Maura H., Perry, Robert L. S., Fauteux, Mélanie C., Berkes, Charlotte A., Rudnicki, Michael A.
The MyoD family of basic helix-loop-helix transcription factors function as heterodimers with members of the E-protein family to induce myogenic gene activation. The E-protein HEB is alternatively...
Severe cardiomyopathy in mice lacking dystrophin and MyoD
Megeney, Lynn A., Kablar, Boris, Perry, Robert L. S., Ying, Chuyan, May, Linda, Rudnicki, Michael A.
The mdx mouse, a mouse model of Duchenne muscular dystrophy, carries a loss-of-function mutation in dystrophin, a component of the membrane-associated dystrophin–glycoprotein complex. Unlike...
Amplification of the neu/erbB-2 oncogene in a mouse model of mammary tumorigenesis
Andrechek, Eran R., Hardy, William R., Siegel, Peter M., Rudnicki, Michael A., Cardiff, Robert D., Muller, William J.
The neu (c-erbB-2, Her-2) protooncogene is amplified and overexpressed in 20–30% of human breast cancers. Although transgenic mouse models have illustrated the role of Neu in the induction of...
Sabourin, Luc A., Seale, Patrick, Wagner, Julian, Rudnicki, Michael A.
We have demonstrated that a novel Ste20-related kinase, designated SLK, mediates apoptosis and actin stress fiber dissolution through distinct domains generated by caspase 3 cleavage. Overexpression...
Male Sexual Dysfunction in Mice Bearing Targeted Mutant Alleles of the PEA3 ets Gene
Laing, Michael A., Coonrod, Scott, Hinton, Barry T., Downie, John W., Tozer, Richard, Rudnicki, Michael A., ...
PEA3, a member of the Ets family of transcriptional regulatory proteins, is expressed in a unique spatial and temporal pattern during mouse embryogenesis; its overexpression is positively correlated...
LeCouter, Jennifer E., Kablar, Boris, Hardy, W. Rodney, Ying, Chuyan, Megeney, Lynn A., May, Linda L., ...
To investigate the function of the Rb-related p107 gene, a null mutation in p107 was introduced into the germ line of mice and bred into a BALB/cJ genetic background. Mice lacking p107 were viable...
ErbB2 Is Required for Muscle Spindle and Myoblast Cell Survival
Andrechek, Eran R., Hardy, William R., Girgis-Gabardo, Adele A., Perry, Robert L. S., Butler, Richard, Graham, Frank L., ...
Signaling mediated by ErbB2 is thought to play a critical role in numerous developmental processes. However, due to the embryonic lethality associated with the germ line inactivation of erbB2, its...
Seale, Patrick, Ishibashi, Jeff, Scimè, Anthony, Rudnicki, Michael A
CD45+:Sca1+ adult stem cells isolated from uninjured muscle do not display any myogenic potential, whereas those isolated from regenerating muscle give rise to myoblasts expressing the paired-box...
Pocket Protein Complexes Are Recruited to Distinct Targets in Quiescent and Proliferating Cells
Balciunaite, Egle, Spektor, Alexander, Lents, Nathan H., Cam, Hugh, Te Riele, Hein, Scime, Anthony, ...
Biochemical and genetic studies have determined that retinoblastoma protein (pRB) tumor suppressor family members have overlapping functions. However, these studies have largely failed to distinguish...
MyoD Synergizes with the E-Protein HEBβ To Induce Myogenic Differentiation
Parker, Maura H., Perry, Robert L. S., Fauteux, Mélanie C., Berkes, Charlotte A., Rudnicki, Michael A.
The MyoD family of basic helix-loop-helix transcription factors function as heterodimers with members of the E-protein family to induce myogenic gene activation. The E-protein HEB is alternatively...
Gene function in early mouse embryonic stem cell differentiation
Sene, Kagnew Hailesellasse, Porter, Christopher J, Palidwor, Gareth, Perez-Iratxeta, Carolina, Muro, Enrique M, Campbell, Pearl A, ...
Oct4 Targets Regulatory Nodes to Modulate Stem Cell Function
Campbell, Pearl A., Perez-Iratxeta, Carolina, Andrade-Navarro, Miguel A., Rudnicki, Michael A.
Stem cells are characterized by two defining features, the ability to self-renew and to differentiate into highly specialized cell types. The POU homeodomain transcription factor Oct4 (Pou5f1) is an...
Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis
Kuang, Shihuan, Chargé, Sophie B., Seale, Patrick, Huh, Michael, Rudnicki, Michael A.
We assessed viable Pax7−/− mice in 129Sv/J background and observed reduced growth and marked muscle wasting together with a complete absence of functional satellite cells. Acute injury resulted...
ChIP on SNP-chip for genome-wide analysis of human histone H4 hyperacetylation
McCann, Jennifer A, Muro, Enrique M, Palmer, Claire, Palidwor, Gareth, Porter, Christopher J, Andrade-Navarro, Miguel A, ...
MyoD induces myogenic differentiation through cooperation of its NH2- and COOH-terminal regions
Ishibashi, Jeff, Perry, Robert L., Asakura, Atsushi, Rudnicki, Michael A.
MyoD and Myf5 are basic helix-loop-helix transcription factors that play key but redundant roles in specifying myogenic progenitors during embryogenesis. However, there are functional differences...
Huh, Michael S., Parker, Maura H., Scimè, Anthony, Parks, Robin, Rudnicki, Michael A.
To investigate the requirement for pRb in myogenic differentiation, a floxed Rb allele was deleted either in proliferating myoblasts or after differentiation. Myf5-Cre mice, lacking pRb in myoblasts,...
Asakura, Atsushi, Hirai, Hiroyuki, Kablar, Boris, Morita, Shigeru, Ishibashi, Jeff, Piras, Bryan A., ...
MyoD is a myogenic master transcription factor that plays an essential role in muscle satellite cell (muscle stem cell) differentiation. To further investigate the function of MyoD in satellite...
Sabourin, Luc A., Girgis-Gabardo, Adele, Seale, Patrick, Asakura, Atsushi, Rudnicki, Michael A.
To gain insight into the regeneration deficit of MyoD−/− muscle, we investigated the growth and differentiation of cultured MyoD−/− myogenic cells. Primary MyoD−/− myogenic cells...
Myogenic specification of side population cells in skeletal muscle
Asakura, Atsushi, Seale, Patrick, Girgis-Gabardo, Adele, Rudnicki, Michael A.
Skeletal muscle contains myogenic progenitors called satellite cells and muscle-derived stem cells that have been suggested to be pluripotent. We further investigated the differentiation potential of...
Megf10 regulates the progression of the satellite cell myogenic program
Holterman, Chet E., Le Grand, Fabien, Kuang, Shihuan, Seale, Patrick, Rudnicki, Michael A.
We identify here the multiple epidermal growth factor repeat transmembrane protein Megf10 as a quiescent satellite cell marker that is also expressed in skeletal myoblasts but not in differentiated...
Bakkar, Nadine, Wang, Jingxin, Ladner, Katherine J., Wang, Huating, Dahlman, Jason M., Carathers, Micheal, ...
Nuclear factor κB (NF-κB) is involved in multiple skeletal muscle disorders, but how it functions in differentiation remains elusive given that both anti- and promyogenic activities have been...