Shimada,Atsushi, Nyitrai,Miklós, Vetter,Ingrid R., Kühlmann,Dorothee, Bugyi,Beáta, Narumiya,Shuh, ...
Shimada, Atsushi, Nyitrai, Miklós, Vetter, Ingrid R., Kühlmann, Dorothee, Bugyi, Beáta, Narumiya, Shuh, ...
Tsiavaliaris,Georgios, Fujita-Becker,Setsuko, Batra,Renu, Levitsky,Dmitrii I., Kull,F. Jon, Geeves,Michael A., ...
Dominant-negative inhibition is a powerful genetic tool for the characterization of gene function in vivo, based on the specific impairment of a gene product by the coexpression of a mutant version...
Pearson,David S., Holtermann,Georg, Ellison,Patricia, Cremo,Christine, Geeves,Michael A.
Reactions involving proteins frequently involve large changes in volume, which allows the equilibrium position to be perturbed by changes in pressure. Rapid changes in pressure can thus be used to...
Pressure jump studies of ADP release from smooth/skeletal muscle S1 (2002)
Pearson,David S., Holtermann,Georg, Ellison,Patricia, Cremo,Christine, Geeves,Michael A.
Toxoplasma gondii myosin A and its light chain: a fast, single-headed, plusend directed motor (2002)
Herm-Götz,Angelika, Weiss,Stefan, Stratmann,Rolf, Fujita-Becker,Setsuko, Ruff,Christine, Meyhöfer,Edgar, ...
Successful host cell invasion is a prerequisite for survival of the obligate intracellular apicomplexan parasites and establishment of infection. Toxoplasma gondii penetrates host cells by an active...
Toxoplasma gondii myosin A and its light chain: a fast, single-headed, plusend directed motor (2002)
Herm-Götz,Angelika, Weiss,Stefan, Stratmann,Rolf, Fujita-Becker,Setsuko, Ruff,Christine, Meyhöfer,Edgar, ...
Successful host cell invasion is a prerequisite for survival of the obligate intracellular apicomplexan parasites and establishment of infection. Toxoplasma gondii penetrates host cells by an active...
Pearson, David S., Holtermann, Georg, Ellison, Patricia, Cremo, Christine, Geeves, Michael A.
Reactions involving proteins frequently involve large changes in volume, which allows the equilibrium position to be perturbed by changes in pressure. Rapid changes in pressure can thus be used to...
Pressure jump studies of ADP release from smooth/skeletal muscle S1 (2002)
Pearson, David S., Holtermann, Georg, Ellison, Patricia, Cremo, Christine, Geeves, Michael A.
Toxoplasma gondii myosin A and its light chain: a fast, single-headed, plusend directed motor (2002)
Herm-Götz, Angelika, Weiss, Stefan, Stratmann, Rolf, Fujita-Becker, Setsuko, Ruff, Christine, Meyhöfer, Edgar, ...
Successful host cell invasion is a prerequisite for survival of the obligate intracellular apicomplexan parasites and establishment of infection. Toxoplasma gondii penetrates host cells by an active...
Tsiavaliaris, Georgios, Fujita-Becker, Setsuko, Batra, Renu, Levitsky, Dmitrii I., Kull, F. Jon, Geeves, Michael A., ...
Dominant-negative inhibition is a powerful genetic tool for the characterization of gene function in vivo, based on the specific impairment of a gene product by the coexpression of a mutant version...
Toxoplasma gondii myosin A and its light chain: a fast, single-headed, plusend directed motor (2002)
Herm-Götz, Angelika, Weiss, Stefan, Stratmann, Rolf, Fujita-Becker, Setsuko, Ruff, Christine, Meyhöfer, Edgar, ...
Successful host cell invasion is a prerequisite for survival of the obligate intracellular apicomplexan parasites and establishment of infection. Toxoplasma gondii penetrates host cells by an active...
Stabilization of the actomyosin complex by negative charges on myosin (2000)
Furch,Marcus, Remmel,Bettina, Geeves,Michael A., Manstein,Dietmar J.
Sequence comparisons of members of the myosin superfamily show a high degree of charge conservation in a surface exposed helix (Dictyostelium discoideum myosin II heavy chain residues S510 to K546)....
Stabilization of the actomyosin complex by negative charges on myosin (2000)
Furch, Marcus, Remmel, Bettina, Geeves, Michael A., Manstein, Dietmar J.
Sequence comparisons of members of the myosin superfamily show a high degree of charge conservation in a surface exposed helix (Dictyostelium discoideum myosin II heavy chain residues S510 to K546)....
Role of the salt-bridge between switch-1 and switch-2 of Dictyostelium myosin (1999)
Furch,Marcus, Fujita-Becker,Setsuko, Geeves,Michael A., Holmes,Kenneth C., Manstein,Dietmar J.
Motifs N2 and N3, also referred to as switch-1 and switch-2, form part of the active site of molecular motors such as myosins and kinesins. In the case of myosin, N3 is thought to act as a...
Batra,Renu, Geeves,Michael A., Manstein,Dietmar J.
Three conserved glycine residues in the reactive thiol region of Dictyostelium discoideummyosin II were replaced by alanine residues. The resulting mutants G680A, G684A, and G691A were expressed in...
Structural Mechanism of Muscle Contraction (1999)
Geeves,Michael A., Holmes,Kenneth C.
X-ray crystallography shows the myosin cross-bridge to exist in two conformations, the beginning and end of the power stroke. A long lever-arm undergoes a 60 degrees to 70 degrees rotation between...
Batra, Renu, Geeves, Michael A., Manstein, Dietmar J.
Three conserved glycine residues in the reactive thiol region of Dictyostelium discoideummyosin II were replaced by alanine residues. The resulting mutants G680A, G684A, and G691A were expressed in...
Role of the salt-bridge between switch-1 and switch-2 of Dictyostelium myosin (1999)
Furch, Marcus, Fujita-Becker, Setsuko, Geeves, Michael A., Holmes, Kenneth C., Manstein, Dietmar J.
Motifs N2 and N3, also referred to as switch-1 and switch-2, form part of the active site of molecular motors such as myosins and kinesins. In the case of myosin, N3 is thought to act as a...
Structural Mechanism of Muscle Contraction (1999)
Geeves, Michael A., Holmes, Kenneth C.
X-ray crystallography shows the myosin cross-bridge to exist in two conformations, the beginning and end of the power stroke. A long lever-arm undergoes a 60 degrees to 70 degrees rotation between...
Kinetic Characterization of myosin head fragments with long-lived myosin ATP states (1998)
Friedman,A. L., Geeves,Michael A., Manstein,Dietmar J., Spudich,J. A.
Furch,Marcus, Geeves,Michael A., Manstein,Dietmar J.
The effects of mutations in an actin-binding surface loop of myosin (loop 2) are described. Part of loop 2, the segment between myosin residues 618 and 622, was replaced with sequences enlarged by...
Levitsky,D. I., Ponomarev,M. A., Geeves,Michael A., Shnyrov,V. L., Manstein,Dietmar J.
The thermal unfolding of two recombinant fragments of the head of Dictyostelium discoideum myosin II was studied by differential scanning calorimetry. These fragments M754 and M761 correspond to the...
Furch, Marcus, Geeves, Michael A., Manstein, Dietmar J.
The effects of mutations in an actin-binding surface loop of myosin (loop 2) are described. Part of loop 2, the segment between myosin residues 618 and 622, was replaced with sequences enlarged by...
Kinetic Characterization of myosin head fragments with long-lived myosin ATP states (1998)
Friedman, A. L., Geeves, Michael A., Manstein, Dietmar J., Spudich, J. A.
Levitsky, D. I., Ponomarev, M. A., Geeves, Michael A., Shnyrov, V. L., Manstein, Dietmar J.
The thermal unfolding of two recombinant fragments of the head of Dictyostelium discoideum myosin II was studied by differential scanning calorimetry. These fragments M754 and M761 correspond to the...
Myosin motors with artificial lever arms (1996)
Anson,Michael, Geeves,Michael A., Kurzawa,Susanne E., Manstein,Dietmar J.
Myosin motors with artificial lever arms (1996)
Anson, Michael, Geeves, Michael A., Kurzawa, Susanne E., Manstein, Dietmar J.
Kinetic characterization of the catalytic domain of Dictyostelium discoideum myosin (1995)
Woodward,S. K., Geeves,Michael A., Manstein,Dietmar J.
The myosin head consists of a globular motor or catalytic domain that contains both the catalytic and actin binding sites, and a neck region which consists of a 8.5 nm alpha-helix that emerges from...
Kinetic characterization of the catalytic domain of Dictyostelium discoideum myosin (1995)
Woodward, S. K., Geeves, Michael A., Manstein, Dietmar J.
The myosin head consists of a globular motor or catalytic domain that contains both the catalytic and actin binding sites, and a neck region which consists of a 8.5 nm alpha-helix that emerges from...
Toxoplasma gondii myosin A and its light chain: a fast, single-headed, plus-end-directed motor
Herm-Götz, Angelika, Weiss, Stefan, Stratmann, Rolf, Fujita-Becker, Setsuko, Ruff, Christine, Meyhöfer, Edgar, ...
Successful host cell invasion is a prerequisite for survival of the obligate intracellular apicomplexan parasites and establishment of infection. Toxoplasma gondii penetrates host cells by an active...
Myo1c is designed for the adaptation response in the inner ear
Batters, Christopher, Arthur, Christopher P, Lin, Abel, Porter, Jessica, Geeves, Michael A, Milligan, Ronald A, ...
The molecular motor, Myo1c, a member of the myosin family, is widely expressed in vertebrate tissues. Its presence at strategic places in the stereocilia of the hair cells in the inner ear and...
Nyitrai, Miklós, Szent-Györgyi, Andrew G, Geeves, Michael A
Analysis of the kinetics of ATP and ADP binding to scallop (Argopecten irradians) heavy meromyosin (HMM) showed that the only calcium-dependent process is the rate of ADP release. At physiological...
Pearson, David S, Holtermann, Georg, Ellison, Patricia, Cremo, Christine, Geeves, Michael A
Reactions involving proteins frequently involve large changes in volume, which allows the equilibrium position to be perturbed by changes in pressure. Rapid changes in pressure can thus be used to...
Nyitrai, Miklos, Szent-Györgyi, Andrew G, Geeves, Michael A
We recently proposed a co-operative model for the influence of calcium and ADP on scallop ( Argopecten irradians ) muscle heavy meromyosin (scHMM), in which scHMM exists in two conformations...
What kinesin does at roadblocks: the coordination mechanism for molecular walking
Crevel, Isabelle M-TC, Nyitrai, Miklós, Alonso, María C, Weiss, Stefan, Geeves, Michael A, Cross, Robert A
Competing models for the coordination of processive stepping in kinesin can be tested by introducing a roadblock to prevent lead head attachment. We used T93N, an irreversibly binding mutant monomer,...
Kremneva, Elena, Boussouf, Sabrina, Nikolaeva, Olga, Maytum, Robin, Geeves, Michael A., Levitsky, Dmitrii I.
Differential scanning calorimetry was used to investigate the thermal unfolding of native α-tropomyosin (Tm), wild-type α-Tm expressed in Escherichia coli and the wild-type α-Tm carrying either of...
Tsiavaliaris, Georgios, Fujita-Becker, Setsuko, Batra, Renu, Levitsky, Dmitrii I., Kull, F. Jon, Geeves, Michael A., ...
Dominant-negative inhibition is a powerful genetic tool for the characterization of gene function in vivo, based on the specific impairment of a gene product by the coexpression of a mutant version...
Tsiavaliaris, Georgios, Fujita-Becker, Setsuko, Batra, Renu, Levitsky, Dmitrii I., Kull, F. Jon, Geeves, Michael A., ...
Cryptococcus neoformans Senses CO2 through the Carbonic Anhydrase Can2 and the Adenylyl Cyclase Cac1
Mogensen, Estelle Gewiss, Janbon, Guilhem, Chaloupka, James, Steegborn, Clemens, Fu, Man Shun, Moyrand, Frédérique, ...
Cryptococcus neoformans, a fungal pathogen of humans, causes fatal meningitis in immunocompromised patients. Its virulence is mainly determined by the elaboration of a polysaccharide capsule...
Toxoplasma gondii myosin A and its light chain: a fast, single-headed, plus-end-directed motor
Herm-Götz, Angelika, Weiss, Stefan, Stratmann, Rolf, Fujita-Becker, Setsuko, Ruff, Christine, Meyhöfer, Edgar, ...
Successful host cell invasion is a prerequisite for survival of the obligate intracellular apicomplexan parasites and establishment of infection. Toxoplasma gondii penetrates host cells by an active...
Myo1c is designed for the adaptation response in the inner ear
Batters, Christopher, Arthur, Christopher P, Lin, Abel, Porter, Jessica, Geeves, Michael A, Milligan, Ronald A, ...
The molecular motor, Myo1c, a member of the myosin family, is widely expressed in vertebrate tissues. Its presence at strategic places in the stereocilia of the hair cells in the inner ear and...
Nyitrai, Miklós, Szent-Györgyi, Andrew G, Geeves, Michael A
Analysis of the kinetics of ATP and ADP binding to scallop (Argopecten irradians) heavy meromyosin (HMM) showed that the only calcium-dependent process is the rate of ADP release. At physiological...
Pearson, David S, Holtermann, Georg, Ellison, Patricia, Cremo, Christine, Geeves, Michael A
Reactions involving proteins frequently involve large changes in volume, which allows the equilibrium position to be perturbed by changes in pressure. Rapid changes in pressure can thus be used to...
Nyitrai, Miklos, Szent-Györgyi, Andrew G, Geeves, Michael A
We recently proposed a co-operative model for the influence of calcium and ADP on scallop ( Argopecten irradians ) muscle heavy meromyosin (scHMM), in which scHMM exists in two conformations...
What kinesin does at roadblocks: the coordination mechanism for molecular walking
Crevel, Isabelle M-TC, Nyitrai, Miklós, Alonso, María C, Weiss, Stefan, Geeves, Michael A, Cross, Robert A
Competing models for the coordination of processive stepping in kinesin can be tested by introducing a roadblock to prevent lead head attachment. We used T93N, an irreversibly binding mutant monomer,...
Kremneva, Elena, Boussouf, Sabrina, Nikolaeva, Olga, Maytum, Robin, Geeves, Michael A., Levitsky, Dmitrii I.
Differential scanning calorimetry was used to investigate the thermal unfolding of native α-tropomyosin (Tm), wild-type α-Tm expressed in Escherichia coli and the wild-type α-Tm carrying either of...
Tsiavaliaris, Georgios, Fujita-Becker, Setsuko, Batra, Renu, Levitsky, Dmitrii I., Kull, F. Jon, Geeves, Michael A., ...
Dominant-negative inhibition is a powerful genetic tool for the characterization of gene function in vivo, based on the specific impairment of a gene product by the coexpression of a mutant version...
Tsiavaliaris, Georgios, Fujita-Becker, Setsuko, Batra, Renu, Levitsky, Dmitrii I., Kull, F. Jon, Geeves, Michael A., ...
Cryptococcus neoformans Senses CO2 through the Carbonic Anhydrase Can2 and the Adenylyl Cyclase Cac1
Mogensen, Estelle Gewiss, Janbon, Guilhem, Chaloupka, James, Steegborn, Clemens, Fu, Man Shun, Moyrand, Frédérique, ...
Cryptococcus neoformans, a fungal pathogen of humans, causes fatal meningitis in immunocompromised patients. Its virulence is mainly determined by the elaboration of a polysaccharide capsule...
Adenosine diphosphate and strain sensitivity in myosin motors.
Nyitrai, Miklós, Geeves, Michael A
The release of adenosine diphosphate (ADP) from the actomyosin cross-bridge plays an important role in the adenosine-triphosphate-driven cross-bridge cycle. In fast contracting muscle fibres, the...
Reversible movement of switch 1 loop of myosin determines actin interaction
Kintses, Bálint, Gyimesi, Máté, Pearson, David S, Geeves, Michael A, Zeng, Wei, Bagshaw, Clive R, ...
The conserved switch 1 loop of P-loop NTPases is implicated as a central element that transmits information between the nucleotide-binding pocket and the binding site of the partner proteins. Recent...
Calcium sensitivity of the cross-bridge cycle of Myo1c, the adaptation motor in the inner ear
Adamek, Nancy, Coluccio, Lynne M., Geeves, Michael A.
The class I myosin Myo1c is a mediator of adaptation of mechanoelectrical transduction in the stereocilia of the inner ear. Adaptation, which is strongly affected by Ca2+, permits hair cells under...