Structure and Expression of Genes for Flavivirus Immunogens. (2002)
Fournier,Maurille J., Mason,Thomas L.
Banks of cloned Japanese encephalitis virus cDNA and monoclonal antibodies specific to virus structural proteins are being used to characterize the structure and expression of the flavivirus genome....
Structure and Expression of Genes for Flavivirus Immunogens. (2002)
Fournier,Maurille J., Mason,Thomas L.
The Japanese encephalitis virus (JEV) genome is being cloned for detailed structure-function studies. Full-length cDNA transcripts have been obtained via reverse transcription of the 12 kb plus...
Structure and Expression of Genes for Flavivirus Immunogens. (1998)
Fournier, Maurille J., Mason, Thomas L.
Banks of cloned cDNA and specific antibodies are being used to characterize the structure and expression of the genomes of two flaviviruses, the Japanese encephalitis virus (JEV) and the dengue-type...
Structure and Expression of Genes for Flavivirus Immunogens. (1998)
Fournier, Maurille J., Mason, Thomas L.
Progress during the reporting period included: mapping of antibody binding sites for the envelope (E) and non-structural-one (NS1) proteins specified by the Japanese encephalitis (JE) and dengue-one...
Structure and Expression of Genes for Flavivirus Immunogens. (1998)
Fournier, Maurille J., Mason, Thomas L.
Progress during the reporting period included: (i) expression of a DEN- virus neutralizing epitope in E. coli (ii) expression of DEN- structural proteins and 80% of the NS protein by a recombinant...
Structure and Expression of Genes for Flavivirus Immunogens. (1998)
Fournier, Maurille J., Mason, Thomas L.
Progress during the report period included: (1) Extension of the nucleotide sequence analysis of the Japanese encephalitis (JE) and dengue serotype 1 (DEN-1) genetic material; (2) expressed in E....
Structure and Expression of Genes for Flavivirus Immunogens. (1998)
Fournier, Maurille J., Mason, Thomas L.
Progress during the reporting period included: (1) Immunization of mice with dengue serotype 1 (DEN-1) structural proteins and a modified form of NS1 expressed in insect cells using a recombinant...
Structure and Expression of Genes for Flavivirus Immunogens. (1998)
Fournier, Maurille J., Mason, Thomas L.
Progress during the report period included: (1) extension of the DEN-1 sequence analysis; (2) napping of binding sites for anti-DEN-l/JE monoclonal antibodies; (3) development of nonradioactive...
Structure and Expression of Genes for Flavivirus Immunogens. (1998)
Fournier, Maurille J., Mason, Thomas L.
As the first step toward expression of full-length copies of the DEN-1 E and PrM proteins in E. coli, cDNA copies of these proteins were amplified by PCR technology and cloned into T7 RNA polymerase...
Biomolecular Materials (Special Report) (1998)
Tirrell, Jane G., Fournier, Maurille J., Mason, Thomas L.
The second half of the 20th century has witnessed a productive interplay between chemistry and materials science - in fields as varied as polymers, catalysis, interface science, ceramics, and...
Liang, Wen-Qing, Fournier, Maurille J.
Active 18S and 25S ribosomal RNAs were produced in trans in yeast, from plasmids containing RNA polymerase II transcription signals and rDNA fragments with unique hybridization tags. Analyses were...
Samarsky, Dmitry A., Ferbeyre, Gerardo, Bertrand, Edouard, Singer, Robert H., Cedergren, Robert, Fournier, Maurille J.
A hammerhead ribozyme has been localized to the yeast nucleolus by using the U3 small nucleolar RNA as a carrier. The hybrid small nucleolar RNA:ribozyme, designated a “snorbozyme,” is...
Point Mutations in Yeast CBF5 Can Abolish In Vivo Pseudouridylation of rRNA
Zebarjadian, Yeganeh, King, Tom, Fournier, Maurille J., Clarke, Louise, Carbon, John
In budding yeast (Saccharomyces cerevisiae), the majority of box H/ACA small nucleolar RNPs (snoRNPs) have been shown to direct site-specific pseudouridylation of rRNA. Among the known protein...
King, Thomas H., Decatur, Wayne A., Bertrand, Edouard, Maxwell, E. Stuart, Fournier, Maurille J.
Biogenesis of small nucleolar RNA-protein complexes (snoRNPs) consists of synthesis of the snoRNA and protein components, snoRNP assembly, and localization to the nucleolus. Recently, two...
Functional Mapping of the U3 Small Nucleolar RNA from the Yeast Saccharomyces cerevisiae
Samarsky, Dmitry A., Fournier, Maurille J.
The U3 small nucleolar RNA participates in early events of eukaryotic pre-rRNA cleavage and is essential for formation of 18S rRNA. Using an in vivo system, we have developed a functional map of the...
Mawn, Mary V., Fournier, Maurille J., Tirrell, David A., Mason, Thomas L.
We have constructed synthetic coding sequences for the expression of poly(α,l-glutamic acid) (PLGA) as fusion proteins with dihydrofolate reductase (DHFR) in Escherichia coli. These PLGA coding...
Schattner, Peter, Decatur, Wayne A., Davis, Carrie A., Ares, Manuel, Fournier, Maurille J., Lowe, Todd M.
One of the largest families of small RNAs in eukaryotes is the H/ACA small nucleolar RNAs (snoRNAs), most of which guide RNA pseudouridine formation. So far, an effective computational method...
Interference probing of rRNA with snoRNPs: A novel approach for functional mapping of RNA in vivo
LIU, BEN, FOURNIER, MAURILLE J.
Synthesis of eukaryotic ribosomal RNAs (rRNAs) includes methylation of scores of nucleotides at the 2′-O-ribose position (Nm) by small nucleolar RNP complexes (snoRNPs). Sequence specificity is...
Liang, Wen-Qing, Fournier, Maurille J.
Active 18S and 25S ribosomal RNAs were produced in trans in yeast, from plasmids containing RNA polymerase II transcription signals and rDNA fragments with unique hybridization tags. Analyses were...
Samarsky, Dmitry A., Ferbeyre, Gerardo, Bertrand, Edouard, Singer, Robert H., Cedergren, Robert, Fournier, Maurille J.
A hammerhead ribozyme has been localized to the yeast nucleolus by using the U3 small nucleolar RNA as a carrier. The hybrid small nucleolar RNA:ribozyme, designated a “snorbozyme,” is...
Point Mutations in Yeast CBF5 Can Abolish In Vivo Pseudouridylation of rRNA
Zebarjadian, Yeganeh, King, Tom, Fournier, Maurille J., Clarke, Louise, Carbon, John
In budding yeast (Saccharomyces cerevisiae), the majority of box H/ACA small nucleolar RNPs (snoRNPs) have been shown to direct site-specific pseudouridylation of rRNA. Among the known protein...
King, Thomas H., Decatur, Wayne A., Bertrand, Edouard, Maxwell, E. Stuart, Fournier, Maurille J.
Biogenesis of small nucleolar RNA-protein complexes (snoRNPs) consists of synthesis of the snoRNA and protein components, snoRNP assembly, and localization to the nucleolus. Recently, two...
Functional Mapping of the U3 Small Nucleolar RNA from the Yeast Saccharomyces cerevisiae
Samarsky, Dmitry A., Fournier, Maurille J.
The U3 small nucleolar RNA participates in early events of eukaryotic pre-rRNA cleavage and is essential for formation of 18S rRNA. Using an in vivo system, we have developed a functional map of the...
Mawn, Mary V., Fournier, Maurille J., Tirrell, David A., Mason, Thomas L.
We have constructed synthetic coding sequences for the expression of poly(α,l-glutamic acid) (PLGA) as fusion proteins with dihydrofolate reductase (DHFR) in Escherichia coli. These PLGA coding...
Schattner, Peter, Decatur, Wayne A., Davis, Carrie A., Ares, Manuel, Fournier, Maurille J., Lowe, Todd M.
One of the largest families of small RNAs in eukaryotes is the H/ACA small nucleolar RNAs (snoRNAs), most of which guide RNA pseudouridine formation. So far, an effective computational method...
Interference probing of rRNA with snoRNPs: A novel approach for functional mapping of RNA in vivo
LIU, BEN, FOURNIER, MAURILLE J.
Synthesis of eukaryotic ribosomal RNAs (rRNAs) includes methylation of scores of nucleotides at the 2′-O-ribose position (Nm) by small nucleolar RNP complexes (snoRNPs). Sequence specificity is...
The Helicase Has1p Is Required for snoRNA Release from Pre-rRNA▿
Liang, Xue-hai, Fournier, Maurille J.
Synthesis of rRNA in eukaryotes involves the action of a large population of snoRNA-protein complexes (snoRNPs), which create modified nucleotides and participate in cleavage of pre-rRNA. The snoRNPs...
A systematic, ligation-based approach to study RNA modifications
Saikia, Mridusmita, Dai, Qing, Decatur, Wayne A., Fournier, Maurille J., Piccirilli, Joseph A., Pan, Tao
Over 100 different chemical types of modifications have been identified in thousands of sites in tRNAs, rRNAs, mRNAs, small nuclear RNAs, and other RNAs. Some modifications are highly conserved,...
New bioinformatic tools for analysis of nucleotide modifications in eukaryotic rRNA
Piekna-Przybylska, Dorota, Decatur, Wayne A., Fournier, Maurille J.
This report presents a valuable new bioinformatics package for research on rRNA nucleotide modifications in the ribosome, especially those created by small nucleolar RNA:protein complexes (snoRNPs)....
The 3D rRNA modification maps database: with interactive tools for ribosome analysis
Piekna-Przybylska, Dorota, Decatur, Wayne A., Fournier, Maurille J.
The 3D rRNA modification maps database is the first general resource of information about the locations of modified nucleotides within the 3D structure of the full ribosome, with mRNA and tRNAs in...