Matthias J. Schnell

Recombinant rabies virus as potential live-viral vaccines for HIV-1

Schnell, Matthias J., Foley, Heather D., Siler, Catherine A., McGettigan, James P., Dietzschold, Bernhard, Pomerantz, Roger J.

Recombinant, replication-competent rabies virus (RV) vaccine strain-based vectors were developed expressing HIV type I (HIV-1) envelope glycoprotein (gp160) from both a laboratory-adapted...

A recombinant rabies virus expressing vesicular stomatitis virus glycoprotein fails to protect against rabies virus infection

Foley, Heather D., McGettigan, James P., Siler, Catherine A., Dietzschold, Bernhard, Schnell, Matthias J.

To investigate the importance of the rabies virus (RV) glycoprotein (G) in protection against rabies, we constructed a recombinant RV (rRV) in which the RV G ecto- and transmembrane domains were...

Generation of mucosal cytotoxic T cells against soluble protein by tissue-specific environmental and costimulatory signals

Kim, Sung-Kwon, Reed, Douglas S., Olson, Sara, Schnell, Matthias J., Rose, John K., Morton, Phillip A., ...

We compared peripheral and mucosal primary CD8 T cell responses to inflammatory and noninflammatory forms of antigen in a T cell-adoptive transfer system. Immunization with the soluble antigen,...

Rhabdoviruses and the Cellular Ubiquitin-Proteasome System: a Budding Interaction

Harty, Ronald N., Brown, Melissa E., McGettigan, James P., Wang, Guangli, Jayakar, Himangi R., Huibregtse, Jon M., ...

The matrix (M) proteins of vesicular stomatitis virus (VSV) and rabies virus (RV) play a key role in both assembly and budding of progeny virions. A PPPY motif (PY motif or late-budding domain) is...

Overexpression of Cytochrome c by a Recombinant Rabies Virus Attenuates Pathogenicity and Enhances Antiviral Immunity

Pulmanausahakul, Rojjanaporn, Faber, Milosz, Morimoto, Kinjiro, Spitsin, Sergei, Weihe, Eberhard, Hooper, D. Craig, ...

The pathogenicity of individual rabies virus strains appears to correlate inversely with the extent of apoptotic cell death they induce and with the expression of rabies virus glycoprotein, a major...

Expression and Immunogenicity of Human Immunodeficiency Virus Type 1 Gag Expressed by a Replication-Competent Rhabdovirus-Based Vaccine Vector

McGettigan, James P., Sarma, Satyam, Orenstein, Jan M., Pomerantz, Roger J., Schnell, Matthias J.

A replication-competent rhabdovirus-based vector expressing human immunodeficiency virus type 1 (HIV-1) Gag protein was characterized on human cell lines and analyzed for the induction of a cellular...

Rhabdovirus-Based Vectors with Human Immunodeficiency Virus Type 1 (HIV-1) Envelopes Display HIV-1-Like Tropism and Target Human Dendritic Cells

Foley, Heather D., Otero, Miguel, Orenstein, Jan M., Pomerantz, Roger J., Schnell, Matthias J.

We describe replication-competent, vaccine strain-based rabies viruses (RVs) that lack their own single glycoprotein and express, instead, a chimeric RV-human immunodeficiency virus type 1 (HIV-1)...

Overexpression of the Rabies Virus Glycoprotein Results in Enhancement of Apoptosis and Antiviral Immune Response

Faber, Milosz, Pulmanausahakul, Rojjanaporn, Hodawadekar, Suchita S., Spitsin, Sergei, McGettigan, James P., Schnell, Matthias J., ...

A recombinant rabies virus (RV) carrying two identical glycoprotein (G) genes (SPBNGA-GA) was constructed and used to determine the effect of RV G overexpression on cell viability and immunity....

Second-Generation Rabies Virus-Based Vaccine Vectors Expressing Human Immunodeficiency Virus Type 1 Gag Have Greatly Reduced Pathogenicity but Are Highly Immunogenic

McGettigan, James P., Pomerantz, Roger J., Siler, Catherine A., McKenna, Philip M., Foley, Heather D., Dietzschold, B., ...

Rabies virus (RV) vaccine strain-based vectors show great promise as vaccines against other viral diseases such as human immunodeficiency virus type 1 (HIV-1) infection and hepatitis C, but a low...

Functional Human Immunodeficiency Virus Type 1 (HIV-1) Gag-Pol or HIV-1 Gag-Pol and Env Expressed from a Single Rhabdovirus-Based Vaccine Vector Genome

McGettigan, James P., Naper, Kristin, Orenstein, Jan, Koser, Martin, McKenna, Philip M., Schnell, Matthias J.

Recombinant rabies virus (RV) vaccine strain-based vectors have been successfully developed as vaccines against other viral diseases (J. P. McGettigan et al., J. Virol. 75:4430-4434, 2001; McGettigan...

Covalently Linked Human Immunodeficiency Virus Type 1 gp120/gp41 Is Stably Anchored in Rhabdovirus Particles and Exposes Critical Neutralizing Epitopes

McKenna, Philip M., Pomerantz, Roger J., Dietzschold, Bernhard, McGettigan, James P., Schnell, Matthias J.

Rabies virus (RV) vaccine strain-based vectors show significant promise as potential live-attenuated vaccines against human immunodeficiency virus type 1 (HIV-1). Here we describe a new RV construct...

Budding of PPxY-Containing Rhabdoviruses Is Not Dependent on Host Proteins TGS101 and VPS4A

Irie, Takashi, Licata, Jillian M., McGettigan, James P., Schnell, Matthias J., Harty, Ronald N.

Viral matrix proteins of several enveloped RNA viruses play important roles in virus assembly and budding and are by themselves able to bud from the cell surface in the form of lipid-enveloped,...

Rabies virus nucleoprotein as a carrier for foreign antigens

Koser, Martin L., McGettigan, James P., Tan, Gene S., Smith, Mary Ellen, Koprowski, Hilary, Dietzschold, Bernhard, ...

Rabies virus (RV) nucleoprotein (N) tightly encapsidates the genomic and antigenomic RNA of RV to form the viral ribonucleoprotein (RNP) complex. Antigens, such as N, presented in a highly organized...

Identification of viral genomic elements responsible for rabies virus neuroinvasiveness

Faber, Milosz, Pulmanausahakul, Rojjanaporn, Nagao, Kazuhiko, Prosniak, Mikhail, Rice, Amy B., Koprowski, Hilary, ...

Attenuated tissue culture-adapted and natural street rabies virus (RV) strains differ greatly in their neuroinvasiveness. To identify the elements responsible for the ability of an RV to enter the...

Immunogenicity Study of Glycoprotein-Deficient Rabies Virus Expressing Simian/Human Immunodeficiency Virus SHIV89.6P Envelope in a Rhesus Macaque

McKenna, Philip M., Aye, Pyone Pyone, Dietzschold, Bernhard, Montefiori, David C., Martin, Louis N., Marx, Preston A., ...

Rabies virus (RV) has recently been developed as a novel vaccine candidate for human immunodeficiency virus type 1 (HIV-1). The RV glycoprotein (G) can be functionally replaced by HIV-1 envelope...

A Single Amino Acid Change in Rabies Virus Glycoprotein Increases Virus Spread and Enhances Virus Pathogenicity

Faber, Milosz, Faber, Marie-Luise, Papaneri, Amy, Bette, Michael, Weihe, Eberhard, Dietzschold, Bernhard, ...

Several rabies virus (RV) vaccine strains containing an aspartic acid (Asp) or glutamic acid (Glu) instead of an arginine (Arg) at position 333 of the RV glycoprotein (G) are apathogenic for...

Overexpression of Tumor Necrosis Factor Alpha by a Recombinant Rabies Virus Attenuates Replication in Neurons and Prevents Lethal Infection in Mice

Faber, Milosz, Bette, Michael, Preuss, Mirjam A. R., Pulmanausahakul, Rojjanaporn, Rehnelt, Jennifer, Schnell, Matthias J., ...

The effect of tumor necrosis factor alpha (TNF-α) on rabies virus (RV) infection of the mouse central nervous system (CNS) was studied, using recombinant RV engineered to express either soluble...

A single immunization with a rhabdovirus-based vector expressing severe acute respiratory syndrome coronavirus (SARS-CoV) S protein results in the production of high levels of SARS-CoV-neutralizing antibodies

Faber, Milosz, Lamirande, Elaine W., Roberts, Anjeanette, Rice, Amy B., Koprowski, Hilary, Dietzschold, Bernhard, ...

Foreign viral proteins expressed by rabies virus (RV) have been shown to induce potent humoral and cellular immune responses in immunized animals. In addition, highly attenuated and, therefore, very...

Both Viral Transcription and Replication Are Reduced when the Rabies Virus Nucleoprotein Is Not Phosphorylated

Wu, Xianfu, Gong, Xiaoming, Foley, Heather D., Schnell, Matthias J., Fu, Zhen F.

Rabies virus nucleoprotein (N) plays vital roles in regulation of viral RNA transcription and replication by encapsidation of the nascent genomic RNA. Rabies virus N is phosphorylated, and previous...

Immunogenicity of Cytopathic and Noncytopathic Viral Vectors

Plesa, Gabriela, McKenna, Philip M., Schnell, Matthias J., Eisenlohr, Laurence C.

The impact of cytolytic versus noncytolytic viral infections on host responses is not well understood, due to limitations of the systems that have been used to address this issue. Using paired...

Recombinant rabies virus as potential live-viral vaccines for HIV-1

Schnell, Matthias J., Foley, Heather D., Siler, Catherine A., McGettigan, James P., Dietzschold, Bernhard, Pomerantz, Roger J.

Recombinant, replication-competent rabies virus (RV) vaccine strain-based vectors were developed expressing HIV type I (HIV-1) envelope glycoprotein (gp160) from both a laboratory-adapted...

A recombinant rabies virus expressing vesicular stomatitis virus glycoprotein fails to protect against rabies virus infection

Foley, Heather D., McGettigan, James P., Siler, Catherine A., Dietzschold, Bernhard, Schnell, Matthias J.

To investigate the importance of the rabies virus (RV) glycoprotein (G) in protection against rabies, we constructed a recombinant RV (rRV) in which the RV G ecto- and transmembrane domains were...

Generation of mucosal cytotoxic T cells against soluble protein by tissue-specific environmental and costimulatory signals

Kim, Sung-Kwon, Reed, Douglas S., Olson, Sara, Schnell, Matthias J., Rose, John K., Morton, Phillip A., ...

We compared peripheral and mucosal primary CD8 T cell responses to inflammatory and noninflammatory forms of antigen in a T cell-adoptive transfer system. Immunization with the soluble antigen,...

Rhabdoviruses and the Cellular Ubiquitin-Proteasome System: a Budding Interaction

Harty, Ronald N., Brown, Melissa E., McGettigan, James P., Wang, Guangli, Jayakar, Himangi R., Huibregtse, Jon M., ...

The matrix (M) proteins of vesicular stomatitis virus (VSV) and rabies virus (RV) play a key role in both assembly and budding of progeny virions. A PPPY motif (PY motif or late-budding domain) is...

Overexpression of Cytochrome c by a Recombinant Rabies Virus Attenuates Pathogenicity and Enhances Antiviral Immunity

Pulmanausahakul, Rojjanaporn, Faber, Milosz, Morimoto, Kinjiro, Spitsin, Sergei, Weihe, Eberhard, Hooper, D. Craig, ...

The pathogenicity of individual rabies virus strains appears to correlate inversely with the extent of apoptotic cell death they induce and with the expression of rabies virus glycoprotein, a major...

Expression and Immunogenicity of Human Immunodeficiency Virus Type 1 Gag Expressed by a Replication-Competent Rhabdovirus-Based Vaccine Vector

McGettigan, James P., Sarma, Satyam, Orenstein, Jan M., Pomerantz, Roger J., Schnell, Matthias J.

A replication-competent rhabdovirus-based vector expressing human immunodeficiency virus type 1 (HIV-1) Gag protein was characterized on human cell lines and analyzed for the induction of a cellular...

Rhabdovirus-Based Vectors with Human Immunodeficiency Virus Type 1 (HIV-1) Envelopes Display HIV-1-Like Tropism and Target Human Dendritic Cells

Foley, Heather D., Otero, Miguel, Orenstein, Jan M., Pomerantz, Roger J., Schnell, Matthias J.

We describe replication-competent, vaccine strain-based rabies viruses (RVs) that lack their own single glycoprotein and express, instead, a chimeric RV-human immunodeficiency virus type 1 (HIV-1)...

Overexpression of the Rabies Virus Glycoprotein Results in Enhancement of Apoptosis and Antiviral Immune Response

Faber, Milosz, Pulmanausahakul, Rojjanaporn, Hodawadekar, Suchita S., Spitsin, Sergei, McGettigan, James P., Schnell, Matthias J., ...

A recombinant rabies virus (RV) carrying two identical glycoprotein (G) genes (SPBNGA-GA) was constructed and used to determine the effect of RV G overexpression on cell viability and immunity....

Second-Generation Rabies Virus-Based Vaccine Vectors Expressing Human Immunodeficiency Virus Type 1 Gag Have Greatly Reduced Pathogenicity but Are Highly Immunogenic

McGettigan, James P., Pomerantz, Roger J., Siler, Catherine A., McKenna, Philip M., Foley, Heather D., Dietzschold, B., ...

Rabies virus (RV) vaccine strain-based vectors show great promise as vaccines against other viral diseases such as human immunodeficiency virus type 1 (HIV-1) infection and hepatitis C, but a low...

Both Viral Transcription and Replication Are Reduced when the Rabies Virus Nucleoprotein Is Not Phosphorylated

Wu, Xianfu, Gong, Xiaoming, Foley, Heather D., Schnell, Matthias J., Fu, Zhen F.

Rabies virus nucleoprotein (N) plays vital roles in regulation of viral RNA transcription and replication by encapsidation of the nascent genomic RNA. Rabies virus N is phosphorylated, and previous...

Functional Human Immunodeficiency Virus Type 1 (HIV-1) Gag-Pol or HIV-1 Gag-Pol and Env Expressed from a Single Rhabdovirus-Based Vaccine Vector Genome

McGettigan, James P., Naper, Kristin, Orenstein, Jan, Koser, Martin, McKenna, Philip M., Schnell, Matthias J.

Recombinant rabies virus (RV) vaccine strain-based vectors have been successfully developed as vaccines against other viral diseases (J. P. McGettigan et al., J. Virol. 75:4430-4434, 2001; McGettigan...

Covalently Linked Human Immunodeficiency Virus Type 1 gp120/gp41 Is Stably Anchored in Rhabdovirus Particles and Exposes Critical Neutralizing Epitopes

McKenna, Philip M., Pomerantz, Roger J., Dietzschold, Bernhard, McGettigan, James P., Schnell, Matthias J.

Rabies virus (RV) vaccine strain-based vectors show significant promise as potential live-attenuated vaccines against human immunodeficiency virus type 1 (HIV-1). Here we describe a new RV construct...

Budding of PPxY-Containing Rhabdoviruses Is Not Dependent on Host Proteins TGS101 and VPS4A

Irie, Takashi, Licata, Jillian M., McGettigan, James P., Schnell, Matthias J., Harty, Ronald N.

Viral matrix proteins of several enveloped RNA viruses play important roles in virus assembly and budding and are by themselves able to bud from the cell surface in the form of lipid-enveloped,...

Rabies virus nucleoprotein as a carrier for foreign antigens

Koser, Martin L., McGettigan, James P., Tan, Gene S., Smith, Mary Ellen, Koprowski, Hilary, Dietzschold, Bernhard, ...

Rabies virus (RV) nucleoprotein (N) tightly encapsidates the genomic and antigenomic RNA of RV to form the viral ribonucleoprotein (RNP) complex. Antigens, such as N, presented in a highly organized...

Identification of viral genomic elements responsible for rabies virus neuroinvasiveness

Faber, Milosz, Pulmanausahakul, Rojjanaporn, Nagao, Kazuhiko, Prosniak, Mikhail, Rice, Amy B., Koprowski, Hilary, ...

Attenuated tissue culture-adapted and natural street rabies virus (RV) strains differ greatly in their neuroinvasiveness. To identify the elements responsible for the ability of an RV to enter the...

Immunogenicity Study of Glycoprotein-Deficient Rabies Virus Expressing Simian/Human Immunodeficiency Virus SHIV89.6P Envelope in a Rhesus Macaque

McKenna, Philip M., Aye, Pyone Pyone, Dietzschold, Bernhard, Montefiori, David C., Martin, Louis N., Marx, Preston A., ...

Rabies virus (RV) has recently been developed as a novel vaccine candidate for human immunodeficiency virus type 1 (HIV-1). The RV glycoprotein (G) can be functionally replaced by HIV-1 envelope...

A Single Amino Acid Change in Rabies Virus Glycoprotein Increases Virus Spread and Enhances Virus Pathogenicity

Faber, Milosz, Faber, Marie-Luise, Papaneri, Amy, Bette, Michael, Weihe, Eberhard, Dietzschold, Bernhard, ...

Several rabies virus (RV) vaccine strains containing an aspartic acid (Asp) or glutamic acid (Glu) instead of an arginine (Arg) at position 333 of the RV glycoprotein (G) are apathogenic for...

Overexpression of Tumor Necrosis Factor Alpha by a Recombinant Rabies Virus Attenuates Replication in Neurons and Prevents Lethal Infection in Mice

Faber, Milosz, Bette, Michael, Preuss, Mirjam A. R., Pulmanausahakul, Rojjanaporn, Rehnelt, Jennifer, Schnell, Matthias J., ...

The effect of tumor necrosis factor alpha (TNF-α) on rabies virus (RV) infection of the mouse central nervous system (CNS) was studied, using recombinant RV engineered to express either soluble...

Immunogenicity of Cytopathic and Noncytopathic Viral Vectors

Plesa, Gabriela, McKenna, Philip M., Schnell, Matthias J., Eisenlohr, Laurence C.

The impact of cytolytic versus noncytolytic viral infections on host responses is not well understood, due to limitations of the systems that have been used to address this issue. Using paired...

The dynein light chain 8 binding motif of rabies virus phosphoprotein promotes efficient viral transcription

Tan, Gene S., Preuss, Mirjam A. R., Williams, John C., Schnell, Matthias J.

Recent studies indicate that the interaction between rabies virus (RV) phosphoprotein and the dynein light chain 8 (LC8) is essential for RV pathogenesis. Through its association with the dynein...

Dominance of a Nonpathogenic Glycoprotein Gene over a Pathogenic Glycoprotein Gene in Rabies Virus▿

Faber, Milosz, Faber, Marie-Luise, Li, Jianwei, Preuss, Mirjam A. R., Schnell, Matthias J., Dietzschold, Bernhard

The nonpathogenic phenotype of the live rabies virus (RV) vaccine SPBNGAN is determined by an Arg→Glu exchange at position 333 in the glycoprotein, designated GAN. We recently showed that after...

The Glycoprotein and the Matrix Protein of Rabies Virus Affect Pathogenicity by Regulating Viral Replication and Facilitating Cell-to-Cell Spread▿

Pulmanausahakul, Rojjanaporn, Li, Jianwei, Schnell, Matthias J., Dietzschold, Bernhard

While the glycoprotein (G) of rabies virus (RV) is known to play a predominant role in the pathogenesis of rabies, the function of the RV matrix protein (M) in RV pathogenicity is not completely...